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Rhizogenesis in in vitro shoot cultures of passion fruit (Passiflora edulis f. flavicarpa Deg.) is affected by ethylene precursor and by inhibitors
47-54.Views:290The effects of the ethylene precursor ACC and two inhibitors, AgNO3 and AVG, on root formation were tested in in vitro shoots of passion fruit (Passiflora Midis f.flavicalpa Deg.). The organogenic response was assessed on the basis of percentage of shoot-forming. roots, root number and length. The time course of ethylene production was also monitored. ACC inhibited root formation by delaying root emergence and increasine, callus formation at the basis of the shoots. In addition, ACC caused a marked increase in ethylene production, coupled to leaf chlorosis and senescence with lower rooting frequencies, number and length of roots. IAA supplementation increased ethylene production. Both ethylene inhibitors, AgNO3 and AVG, at appropriate concentrations reduced callus formation at the basis of shoots. AVG increased the number of roots per shoot, but drastically reduced length of differentiated roots. Regarding to leaf pigments, ACC promoted a marked reduction on carotenoids and total chlorophyll, whereas AVG and AgNO3 delayed explant senescence and pigments degradation, not differing from IAA supplemented and non-supplemented control treatments. The results confirm previous reports on the beneficial effects of ethylene inhibitors on in vitro rooting and suggest its reliability to be used as an alternative approach to evaluate sensitivity of Passiflora species to ethylene.
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Effects of salinity stress induced by hot spring water on tomato growth, yield and fruit quality under hydroponic cultivation in Japan
23-28.Views:312The objective of this research was to test hot spring water as a source of salt to improve tomato quality under the hydroponic system. This research was conducted at Yamagata University, in Japan from February to July 2017. Salt stress was induced using salts of hot spring wastewater collected from Yupoka Onsen (Tsuruoka, Japan). The treatments were EC 2, 4, 8 12, and 16 mS/cm which were arranged in a Randomized Complete Block Design (RCBD) with five replications. Tomato plants were grown at EC 2 until flowering and then subjected to different EC concentrations until harvesting. The data were collected on plant growth parameters and fruit quality. Fruits were harvested at the red stage until the 5th truss. The results showed that fruits' Soluble Solids Content, organic acid, Nitrate contents and Sugar: Acid ratio increased significantly at EC 16 mS/cm and in the upper trusses compared to EC 2 and in the lower trusses. In contrast, fruit weight significantly decreased at EC 16 mS/cm and in upper trusses. Leaf thickness, size and SPAD, and specific leaf weight significantly declined at EC 16 mS/cm and upper leaves compared to EC 2 and in lower leaves. Plant height started to decline significantly after three weeks of treatment at EC 16 compared to EC 2.
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Bioactive phenols in leaves of Forsythia species
57-59.Views:403Nowadays a number of lignans (arctigenin, matairesinol, pinoresinol and phillygenin) have come to the fore in research due to their various biological activities. In this paper the accumulation of these constituents in leaf extracts of Forsythia plants (F. intermedia, F. ovata 'Robusta’ and `Tetragold', F. suspensa, F. viridissima) was quantified using a new isolation method, supercritical CO2 fluid extraction. The total phenolic and flavonoid contents, the antioxidant capacity and the aglycone lignan profile were determined in leaf extracts of Forsythia species. Within the phenols, the flavonoids were only present in small quantities, but the amount of aglycone lignans was extremely high. F. ovata `Robusta' had the highest total lignan content (103.8 mg/g) of all the Forsythia species. The main lignan in this species is arctigenin, which normally makes up about 60% of the total lignan content, but in the case of F. ovata `Robusta' this value was 96.1%. Since this arctigenin content is outstanding compared to that of other Forsythia species, it could be promising to develop a fermentation technology for the production of this natural compound.
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Effect of nitrogen dressings to Jonathan apple trees in a long-term experiment
128-130.Views:295During the last three decades, diverse effects of nitrogen application on the performance of apple trees were studied in field and pot experiments at the Experimental Station of University of Horticulture and Food Industry. The basic experiment, using different rates of nitrogen in kg/ha (check, N-50; N-100; N-200; N-400; and N-800), was carried out for a period of 13 years, thus including almost the whole bearing period of Jonathan apple trees on M.9 rootstocks.
The need of nitrogen in apple orchards on M.9 rootstock and soils with moderate humus content can be decreased considerably. Nitrogen application significantly increased leaf nitrogen and magnesium, but depressed leaf phosphorus and potassium content. With increasing doses of nitrogen fruit nitrogen content significantly increased and parallelly phosphorus and potassium content decreased. Even the lowest rate of nitrogen application decreased the red colouration of fruits. A direct negative correlation between nitrogen fertilzation and fruit firmness was not proved. No close and significant correlation between fruit quality parameters and the nitrogen content in leaves, sampled at different dates, was revealed.
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Genetic engineering of apple (Malus domestica Borkh.) for resistance to fungal diseases using g2ps1 gene from Gerbera hybrida (Asteraceae)
15-12.Views:420In the present study, g2ps1 gene from Gerbera hybrida coding for 2-pyrone synthase which contribute for fungal and insect resistance was used. The aim was to work out an efficient approach of genetic transformation for apple cvs. ‘Golden Delicious’, ‘Royal Gala’ and ‘MM111’, ‘M26’ rootstocks for improving their fungal resistance using genetic engineering techniques. Adventitious shoot formation from leaf pieces of apples studied was achieved using middle leaf segments taken from the youngest leaves from in vitro-grown plants.
Optimum conditions for ‚direct’ shoot organogenesis resulted in high regeneration efficiency of 0%, 95%, 92%, 94% in the studied apples respectively. Putative transgenic shoots could be obtained on MS media with B5 Vitamins, 5.0 mg l-1 BAP, or 2.0 mg l-1 TDZ with 0.2 mg l-1 NAA in the presence of the selection agent “PPT” at 3.0-5.0 mgl-1. Shoot multiplication of transgenic shoots was achieved on: MS + B5 vitamins + 1.0 mg l-1 BAP + 0.3 mg l-1 IBA, 0.2 mg l-1 GA3+1.0 g/l MES+ 30 g/l sucrose + 7.0 g/l Agar, with the selection agent PPT at 5.0 mg l-1 and were subcultured every 4 weeks in order to get sufficient material to confirm transformation of the putative shoots obtained. Six, seven, one and six transgenic clones of the apples studied respectively have been obtained and confirmed by selection on the media containing the selection agent “PPT” and by PCR analysis using the suitable primers in all clones obtained for the presence of the selection” bar gene (447 bp) and the gene-of- interest “g2PS1” (1244 bp), with transformation efficiency of 0.4%, 0.6%, 0.1% and 0.3% respectively. These transgenic clones were multiplied further in vitro in the presence of the selection agent ‘PPT’ and rooted in vitro. Rooted transgenic plantlets were successfully acclimatized and are being kept under-containment conditions according to the biosafety by-law in Syria to evaluate their performance for fungal resistance . -
Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia
46-52.Views:347The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha-1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.
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Effect of boron nutrition on nutrient uptake and fruit quality of tart cherry in Eastern Hungary
45-48.Views:738Effect of the foliar boron (B) application on nutrient uptake and fruit quality of tart cherry (Prunus cerasus L.) was investigated from 2008 to 2009 on mature four favourite Hungarian tart cherry cultivars like ‘Oblacsinszka’, ‘Érdi bôtermő’, ‘Újfehértói fürtös’ and ‘Petri’. Tart cherry trees grown in Eastern Hungary (Újfehértó) on an acidic sandy soil with low B content. Trees were treated with B by foliar application (0.5% B) at full bloom and untreated with B served as a control. B sprays strongly affected on B content of inflorescences. However, B sprays had no consistent effect on summer leaf B status. In our experiment stronger year effect was observed at leaf B status than flower B status. The effectiveness of early spring boron applications are limited and mostly affected the flower B status only. Moreover, treatments had inconsistent effect on studied fruit inner parameters. Monosaccharides, vitamin C and organic acid contents of sour cherry were stronger affected by cultivars than applied treatments.
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Inter-incompatibility of self- incompatible apricots and their varietal properties
79-81.Views:1120There are four apricot varieties grown in Hungary derived from local selections known to bear fruits of giant (60 - 100 g) size: Ceglédi óriás, Nagykőrösi óriás, Szegedi mammut and Ligeti óriás. Being morphologically similar, they seem to be closely related to each other. The detailed study of the morphology (of leaves and fruits) and phenology (of blooming and ripening dates) as well as the fertility relations was aimed to find out the degree of kinship between the varieties in question.
It was stated that the value of morphological traits is variable from the taxonomic point of view. The most important signs of common origin were the time of blooming and the leaf size. Less valuable are the date of m:iurity and the size of fruit because of their variability. In the literature Satin') & Nyeki (1991) published the first proof of inter-incompatible relation between apricot varieties. This should be considered as an argument of close genetic relation between those "giant" varieties of apricots.
The first three varieties. Ceglédi óriás, Nagykőrösi óriás and Szegedi mammut are closer related in blooming and ripening date, as well as in size of fruit to each other than the variety Ligeti óriás.
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Impact of boron foliar fertilization on annual fluctuation of B in sweet cherry leaves and fruit quality
27-30.Views:1017The goal of the study was to examine response of sweet cherries (Prunus avium L.) to boron (B) fertilization. The experiment was conducted during 2005-2007 in West Hungary on mature cv. `Germersdorfi 3' grafted on Prunus mahaleb rootstock.
Sweet cherry trees planted on a calcareous chernozem soil. Trees were foliar-fertilized with B. Foliar B sprays were performed: (1) in the spring, at the stage of white bud, beginning of flowering (B1), and (2) repeated 5 weeks after full bloom (B2). In each of spring spray treatments, B was applied at a rate of 0.15 kg ha-I. Trees untreated with B served as a control.
The results showed that B fertilization had effect on B concentration in leaf tissues, mostly after ripening. B was present significantly higher amount in leaf in treated samples after ripening.
Mean fruit weight was slightly increased by B fertilization. Fruit sensitivity to cracking was not influenced by B fertilization. Nevertheless, from our data it can be conclude that the sensitivity of fruit to cracking is improved when the fruit is riper, the fruit density and fruit weight are higher. The soluble solids varied between 15.0 and 15.9% according to the treatments. Our results for the monosaccharides investigated varied between 5.1 and 7.2 as glucose and fructose as well. Galactose and sucrose was detected very small amount in the unprocessed cherries. Applied B treatments increased sugar contents but decreased organic acid contents in sweet cherry fruits.
It is concluded that under conditions of this experiment, B fertilization can be recommended in sweet cherry culture to improve fruit quality and their appearance.
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Impact of different growing media on propagation of bougainvillea (Bougainvillea spp.) through hardwood stem cuttings in Chitwan, Nepal
37-43.Views:671An experiment was conducted in Rampur, Chitwan, Nepal, in a Randomized Complete Block Design (RCBD) with five treatments: T1 (sand:soil, 1:1) [control], T2 (sand:cocopeat:perlite, 1:1:1), T3 (perlite:sand, 1:1), T4 (sand:cocopeat, 1:1), and T5 (peat moss:perlite, 1:1). Each treatment was replicated four times with a total of 300 planted cuttings. The key parameters evaluated were sprouting percentage, shoot growth (length, leaf number, and branch number), and root development (length, number, fresh, and dry biomass). Analysis of data was done using R-Studio, and Duncan’s Multiple Range Test (DMRT) was performed at a 5% significance level. The results revealed that peat moss:perlite (1:1) demonstrated the highest shoot growth with shoot length of 6.00, 14.38 and 28.78 cm at 30, 60, and 90 Days After Planting (DAP) respectively. Leaf number were 6.25, 13.12, and 19.57, while branch numbers were 1.76, 2.00, and 2.18 at the same intervals., Additionally, the fresh root weight was recorded 8.74 g. Sand:cocopeat:perlite (1:1:1) recorded the highest sprouting percentage (81.66%) and root length (17.11 cm), while sand:cocopeat (1:1) achieved the highest root number (18.37) and dry root weight (0.99 g). Perlite:sand (1:1) exhibited moderate performance with sprouting percentage 69.99%, and root length 12.56 cm. In contrast, control (sand:soil) showed the least favorable results with the lowest sprouting percentage (58.33%), the fewest number of roots (7.81), and the minimum root length (9.73). This study concludes that peat moss:perlite (1:1) is the most effective growing medium for bougainvillea propagation, offering a practical solution to enhance rooting success and growth performance. Sand:cocopeat (1:1) with maximum dry weight of root and maximum number of roots emerged as a reliable alternative, proving the effectiveness of the medium.
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Fruit drop: II. Biological background of flower and fruit drop
103-108.Views:968The most important components of fruit drop are: the rootstock, the combination of polliniser varieties, the conditions depending of nutrition, the extent and timing of the administration of fertilisers, the moments of water stress and the timing of agrotechnical interventions. Further adversities may appear as flushes of heat and drought, the rainy spring weather during the blooming period as well as the excessive hot, cool or windy weather impairing pollination, moreover, the appearance of diseases and pests all influence the fate of flowers of growing and become ripe fruits. As generally maintained, dry springs are causing severe fruit drop.
In analysing the endogenous and environmental causes of drop of the generative organs (flowers and fruits), the model of leaf abscission has been used, as a study of the excised, well defined abscission zone (AZ) seemed to be an adequate approach to the question. Comparing the effects active in the abscission of fruit with those of the excised leaf stem differences are observed as well as analogies between the anatomy and the accumulation of ethylene in the respective abscission tissues.
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Evaluation of fruit tree transpiration with heat flux sensor
11-13.Views:368One of the biggest professional challenges of the following years is to develop the water resource management for apple trees. In order to know the water consumption of plants, some traditional and modern methods could useful. In our research the sap flow measurement of the plants were carried out. We have analyzed the transpiration properties of the trees in the investigation period. In order to compare the transpiration of the trees, the leaf areas were scanned and evaluated. Beside these measurements, soil water properties were determined by tensiometers, thus it makes possible to observe the soil-plant-atmosphere system.
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Role of organosilicone surfactant in apple scab control under scab conducive weather conditions
23-25.Views:680Fungicides of integrated fruit production (dithianon, captan, and diclofluanid) and an organosilicone surfactant were compared in spray schedules from green tip until summer in order to control apple scab caused by Venturia inaequalis and to evaluate their phytotoxicity on fruit. Sixteen sprays of 1.8 kg ha-1captan, 0.41 ha-1 dithianon, and 1.8 kg ha-1 diclolfluanid significantly (P=0.05) reduced the incidence of leaf or fruit scab compared to unsprayed products. All fungicides applied with organosilicone at 0.1% resulted in lower incidence of scab on young and older leaves as well as on harvested fruit, but these were not statistically always better than fungicides applied alone. In case of diclofluanid, the fungicide applied with organosilicone at 0.1% resulted in significantly lower (P = 0.001) incidence of scab on young and older leaves. Diclofluanid applied with organosilicone at 0.1% gave the best scab control on leaf and fruit. Treatments applied with fungicides alone had no significant effect on plant phytotoxicity compared to untreated control. All fungicides applied with organosilicone at 0.1% increased (P = 0.05) fruit damage compared to untreated control or fungicide applied alone, though these were not always significantly different. In case of percentage of fruit russet, treatment of dithianon 0.4 1/ha + 0.1% organosilicone significantly increased fruit russet, while fruit russet index significantly increased in the treatment of diclofluanid 1.8 kg/ha + 0.1% organosilicone compared to untreated control. In sum, application of surfactants can help to increase efficacy of scab fungicides; and consequently, to reduce the risk of fungus infection under high scab disease pressure. This fact may also be helpful in fungicide resistance management and reduced-spray programs with accurate scab warning systems.
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Changing of carbohydrates by inoculation of Pseudomonas savastanoi pv. phaseolicola oil bean lines with different resistance
82-85.Views:556The Pseudomonas savastanoi pv. phaseolicola (PS) is one of the most significant stressors of bean (Phaseolus vulgaris L.). Chemical and agrotechnical treatments have minor importance, so breeding has great part in the protection against this pathogen. Most of the cultivars are susceptible to PS. The genetic background of resistance in the plant is a complex system. Leaf resistance is a monogenic system, but there are some modifier genes. The pathogen species can be divided into different races.
To understand the functioning of this resistance gene, experiments were carried out using bean varieties with different genotypes and near isogenic lines of bean. Eight lines were tested. Our main objective was to test bean lines with PS with high virulence.
The experiment was made in greenhouse and on field. The virulent bacterium strain has been isolated in Hungary.
The changes of carbohydrates were tested after infection. In homeostasis the level of carbohydrates (especially glucose and fructose) were higher in susceptible lines. In case of artificial and natural infection the decrease of glucose were more significant in susceptible lines than in resistant lines. In the leaf samples from systemic chlorosis the level of this carbohydrate increased.
These changes are connected with the level of resistance, but more experiments are needed to verify this assumption.
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Effect of different flower thinning techniques on annual fluctuation of macro and micronutrients in sweet cherries
87-90.Views:874The seasonal dynamic of macro- and micronutrients uptake of a sweet cherry cultivar cv. 'Katalin' (Prunus avium L.) was studied according to apply different flower thinning techniques. Beside control treatment, three thinning treatments were performed: (I) thinning for I flower/inflorescence, (2) thinning for 2 flower/inflorescence, (3) thinning for 3 flower/inflorescence. Soil examination was carried out to establish the growing conditions of orchard site. Moreover, for studying the temporal dynamic of nutrient uptake plant analytical examination was performed four times per year based on leaf collecting according to the phenological phases. It was found that the macro- and micronutrients contents of leaves were showed significant differences between treatments before ripening, at ripening and after ripening stage. It was found that thinning has influence on mineral composition of leaf. Flower removal unbalanced the equilibrium of generative and vegetative processes. The applied manual flower thinning treatment resulted improving vegetative processes like nutrient uptake and storage.
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Prevalence of banana diseases and post-harvest losses in Kenya, and biocontrol potential of arbuscular mycorrhizal fungi against Fusarium wilt
62-73.Views:775The demand for bananas (Musa spp.), which is ranked as the most important fruit crop in Kenya has been on the rise owing to both their dietary contribution and income generation. Meeting this demand has however been hampered by losses during production or post-harvest. This study assessed banana disease and post-harvest losses in leading producing counties in Kenya namely; Kisii, Nyamira and Embu. The study also assessed the efficacy of Rhizophagus irregularis in controlling Fusarium oxysporum f.sp. cubense. Structured questionnaires were used to collect data on post-harvest losses. Disease scoring tables, charts and photos were used to confirm observed symptoms and hence, disease occurrence and severity. AMF biocontrol efficacy experiment was conducted using tissue culture bananas grown in the greenhouse. The study revealed that most smallholder farmers were unaware of the causes or the prevalence of post-harvest losses. The findings also revealed a significant difference (p<0.05) in the severity of banana diseases across various cultivars from the three counties. The AMF treated bananas showed a significant difference (p<0.05) in plant height, total leaf area and chlorosis in comparison to other treatments. The study also revealed a reduction of Fusarium’s pathogenic effects including chlorosis, reduced leaf surface area and eventual necrosis.
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Effect of water supply on nutrient transport in grapevine varieties
64-68.Views:264The effect of water supply on availability of macro nutrient elements (N, P, K) by the plants in the soil and their transport in the plants were examined. In a field experiment two grapevine varieties characterized by higher (White Riesling B 7) and lower (Kövidinka K 8) water requirement were compared on the basis of N, P, K concentrations of leaf blades, petioles and berries. A different water supply of the vineyard was achived by striped coverage of the soil with plastic foil to exclude rainfalls from the beginning of May to ripening. Humidity of the soil decreased as the vegetative phase advanced. Soil cover resulted in 25-30% decrease of the water content in the soil at flowering but this difference gradually disappeared till veraison (i.e. the start of intense growth of the berries). The water consumption in the White Riesling B 7 plantation was more intensive. Mobility and availability of N, P, K in the soil was restricted by water exclusion (i.e. plastic soil cover) at flowering. Nitrogen was slightly affected, whereas P and K were in a higher extent. Comparing the transport of nutrient elements in the two varieties, leaf blades of Kövidinka K 8 contained less N and more P and K than White Riesling B 7 at flowering and more N and P and less K at ripening. Water deficiency inhibited K accumulation in the berries of White Riesling B 7, while this effect did not appear in Kövidinka K 8.Water exclusion decreased the yield of White Riesling B 7 already at lower bud loading, the yield of Kövidinka K 8 was affected only at higher bud loading. The higher yields of the treatments in Kövidinka K 8 plantation support the superior performance of this variety under the hot and dry climate of the Hungarian Great Plain.
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Incidence of two leaf fungal diseases in two plum training systems
15-17.Views:1155In a two-year study, we aimed to determine the susceptibility of four plum cultivars to two fungal pathogens of plum (Stigmina carpophila and Polystigma rubrum) in two training systems with tree spacings of 4 x 1.5m and 6 x 3m. Results showed that shothole symptoms were not detected on cvs ’Bluefre’ and ’Stanley’ in August, 2016. Disease incidence was above 50% in the case of ’Čačanska lepotica’ in both training systems in 2016. There were no significant difference between the two training systems. Shot hole incidence was lower in the 6 x 3m spacings compared to the 4 x 1.5m spacings on cv ’President’ in 2016. Cultivar ’Čačanska lepotica’ showed the highest incidence of Stigmina carpophila in the 4 x 1.5m spacing in 2017. Disease incidence of Stigmina carpophila was significantly lower in the 6 x 3m spacing compared to the 4 x 1.5m spacing. Shothole incidences on cv ’President’ were similar to the values in 2016 ranging from 40% to 60%. Leaf disease incidence was higher in the 4 x 1.5m spacing compared to the 6 x 3m plot. Low disease incidence (below 10%) was observed on cv ’Stanley’ in 2017 and only in the 4 x 1.5m spacing. There were no visible symptoms of blackhorn dotty in 2016 due to inadequate weather conditions for the Polystigma rubrum fungus. However, all the four cultivars were infected by Polystigma rubrum in 2017. The most susceptible cultivar was cv ’Čačanska lepotica’ with the highest disease incidence in the 4 x 1.5m spacing. Disease incidence of this cultivar was lower in the 6 x 3m spacing which was significantly less than in the 4 x 1.5m spacing. The least susceptible cultivar was ’Bluefre’ and symptoms were observed only in the spacing of 4 x 1.5m. The disease incidence of cv ’President’ was similarly low to cv ’Stanley’ in both spacings.
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Effect of various growing media on growth and yield of lettuce in hydroponics system at Lalitpur, Nepal
41-47.Views:171Hydroponic farming, particularly using the nutrient film technique, is gaining traction as a sustainable approach for cultivating leafy vegetables such as lettuce. However, the selection of appropriate growing media remains critical for optimizing plant growth and yield in a soilless system. A completely randomized design (CRD) was used in this study to assess the impact of six distinct growing media (sponge made up of cellulose, perlite, saw dust, sand, cocopeat, and rice husk) on the growth and yield of lettuce under NFT conditions at Mutha Agro Pvt. Ltd., Lalitpur, Nepal. Analysis of variance (ANOVA) in RStudio (v4.4.1) was used to examine the data, and Tukey HSD test was used to differentiate treatment means at a 5% significance level. Lettuce grown in sponge media exhibited the highest performance in terms of number of leaves (11), longest leaf length (17.55 cm), greatest leaf width (9.43cm), plant height (35.81 cm), root length (12.51 cm), canopy diameter (36.07 cm), and stem diameter (0.62 cm). The highest fresh root weight (24.67 g) and moisture loss (98.7%) were obtained by perlite. The highest yield was recorded in sponge (146.45 g), followed by perlite (139.65 g), while the lowest was observed in rice husk (117.83 g). Overall, sponge made up of cellulose proved to be the most effective growing medium for hydroponic lettuce cultivation using NFT, followed by perlite. These findings can help guide growers in selecting suitable substrates to improve yield and quality in hydroponic production systems.
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The in vitro and in vivo anatomical structure of leaves of Prunus x Davidopersica ‘Piroska' and Sorbus rotundifolia L. ‘Bükk szépe'
92-95.Views:523Immature in vitro leaves showed similar structure of the mesophyll tissue to the immature field-grown (in vivo) leaves of Prunus x davidopersica `Piroska'. Mature leaf anatomical characteristics of in vitro plantlets differ from the field-grown plants. The mesophyll tissue of in vitro plantlets were thinner than the in vivo plants and consisted of only one layer palisade parenchyma, the shape of the cells and the structure of spongy parenchyma basically differed from the field-grown plants. In the case of Sorbus rotundifolia similar anatomical differences were found both in vitro and in vivo as in the case of Prunus x davidopersica `Piroska'.
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Effect of frost damage on leaf macronutrient status of eight apple cultivars in integrated apple orchard in Eastern-Hungary
37-40.Views:5372The year of 2007 was critical for fruit growers in the region of Easter-Hungary. Several orchards were suffered frost damages. Significant frost damage was also happened in the orchard of Tedej Rt., which caused total fruit failure in the orchard. Our study was conducted in the integrated apple orchard (Malus domestics Borkh.) established on a lowland chemozem soil in East-Hungary, to investigate how frost changes the mineral content of different apple species. Leaf samples were collected 100 days after full bloom both in 2006 and 2007. The concentrations of nitrogen, phosphorous, potassium, calcium and magnesium were measured in leaves. The absence of fruits caused a smaller accumulation of nitrogen and magnesium in leaves. Falling down of flowers and fruit sets hindered the translocation of the uptaken phosphorous, potassium and calcium towards fruit sets. Due to fruit failure the vegetative processes became dominant. Leaves larger amount of phosphorous, potassium and calcium stored. Besides the absolute element content, the ratio of the different elements was also determined. Majority of calculated ratios were removed from optimal values due to frost. Both absolute content of nutrients and their ratios pointed out that the frost damage significantly affected the uptake of nutrients and their storing processes.
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Effect of straw and plastic mulches on growth and yield of zero tillage potato (Solanum tuberosum L.) in the Coastal Khulna Region of Bangladesh
23-30.Views:324A randomized complete block design experiment was undertaken at the field laboratory of the Agrotechnology Discipline, Khulna University, Khulna, from November 2021 to March 2022 to ascertain the impact of various mulch treatments on zero tillage potato cultivation. The experiment employed BARI Alu 78 as the planting material and utilized three replications with five distinct mulch treatments. These mulch treatments were identified as follows: Straw mulch (T1), Mulch film (T2), Black polythene (T3), White polythene (T4), and Blue polythene (T5). The metrics that were recorded included the number of sprouts, plant height, number of leaves per plant, leaf width, leaf length, number of tubers, tuber length, tuber breadth, number of defective tubers, weight of defective tubers, and tuber weight. Different mulching techniques significantly influenced the overall crop yield and the underlying factors that influenced the results. The mulch film treatment resulted in the highest yield, with a production of 34.76 tons per hectare, followed by black polythene (28.11 tons per hectare), straw mulch (23.74 tons per hectare), white polythene (20.44 tons per hectare), and blue polythene (18.66 tons per hectare). The economic analysis revealed that mulch film had a high benefit cost ratio along with high input and output. Black polythene came in second place when the benefit cost ratio was compared to that of mulch film and the rest of the three treatments. Combining zero tillage with mulch film or black polythene demonstrates significant potential for becoming a cost-effective practice in the coastal region of Bangladesh.
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Effect of a nanotechnology-based foliar fertilizer on the yield and fruit quality in an apple orchard
29-32.Views:1227Nutrient management is a determining element of the technology in fruit production. Significance of foliar fertilization has been increased continuously over the last years, as it can improve directly the vegetative and generative performance of the trees. In this study we aimed to evaluate the effect of a nanotechnology-based foliar fertilizer (Bistep) with 1, 3, and 5 l/ha dosages on the yield and fruit quality parameters in an apple orchard during 2016 and 2018. According to our results, crop load increased with 29% in the third year of the experiment with the application of 5 l/ha Bistep treatment compared to the control treatments. Fruit weight was also improved in each year, as values of fruit weight in all treatments were higher than the control one (3.0-13.0% growth). Fruit surface color increased with 2-18% due to the foliar fertilizer. During the three years, leaf length was 9.5-9.9 mm on the control trees, as 9.8-10.4 mm was measured on the fertilized ones. In conclusion, yield and fruit quality can be improved in apple orchard using a nanotechnology-based foliar fertilizer.
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The evaluation of grape vine decline pathogens in the experimental field of the Georgikon Faculty of Agriculture in Cserszegtomaj
19-22.Views:321Vine decline causes important economic loss in viticulture, especially in longer view. Several causal pathogen were described lately, however little is known about the etiology or epidemiology of these pathogens on grapevine rootstock. It is well known that these diseases affect grafted and rooted grapevines and are not specific to any scion-rootstock combinations. Our aim was to determine what pathogens are presents in the experimental field, especially causal agents of the rootstock decline. Different grapevine rootstocks and scion varieties were tested in our trial. Isolations were made from the wood tissue and pathogenity tests were done with isolated Cylindrocarpon destructans. The possibility of infection during the propagation process was studied as well. Most commonly Cylindrocarpon sp. and Phomopsis sp. species were identified from the examined varieties. Cylindrocarpon destructans was able to spread to apical (shoot) and basal (root) direction from the point of infection with uneven speed. Callus development is not inhibited by the fungi causing the leaf symptom of the vine decline. Shoot development is reduced if unhealthy parts are grafted to each other.
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Changes of the characteristics of Satureja hortensis L. herb during flowering period
56-60.Views:264In this study the changes of the characteristics of Satureja hortensis herb were investigated during flowering period, in case of savories of 4 different origins. The change of drying ratio, leaf/stem ratio, essential oil content, the ratio of several essential oil components (carvacrol, y-terpinene, p-cymol, a-pinene, p-pinene and fi-caryophyllene) were studied from bud formation till seed ripening.
