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International Journal of Horticultural Science

Vol 30 (2024)
Articles

Prevalence of banana diseases and post-harvest losses in Kenya, and biocontrol potential of arbuscular mycorrhizal fungi against Fusarium wiltngi Against Fusarium wilt

Published:
2024-07-16
DOI Linkhttps://doi.org/10.31421/ijhs/30/2024/13781
Authors
H. K. Kamore,
E. M. Njeru,
S. B. Nchore,
R. O. Ombori,
J. M. Muthini,
J. Kimiti
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Keywords
Arbuscular mycorrhizal fungi Musa spp Post-harvest losses Fusarium wilt Rhizophagus irregularis Sigatoka.
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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

How To Cite
Selected Style: APA
Kamore, H. K., Njeru, E. M., Nchore, S. B. ., Ombori, R. O., Muthini, J. M., & Kimiti, J. (2024). Prevalence of banana diseases and post-harvest losses in Kenya, and biocontrol potential of arbuscular mycorrhizal fungi against Fusarium wiltngi Against Fusarium wilt. International Journal of Horticultural Science, 30(1), 62-73. https://doi.org/10.31421/ijhs/30/2024/13781
Abstract

The demand for bananas (Musa spp.), which is ranked as the most important fruit crop in Kenya has been on the rise owing to both their dietary contribution and income generation. Meeting this demand has however been hampered by losses during production or post-harvest. This study assessed banana disease and post-harvest losses in leading producing counties in Kenya namely; Kisii, Nyamira and Embu. The study also assessed the efficacy of Rhizophagus irregularis in controlling Fusarium oxysporum f.sp. cubense. Structured questionnaires were used to collect data on post-harvest losses. Disease scoring tables, charts and photos were used to confirm observed symptoms and hence, disease occurrence and severity. AMF biocontrol efficacy experiment was conducted using tissue culture bananas grown in the greenhouse. The study revealed that most smallholder farmers were unaware of the causes or the prevalence of post-harvest losses. The findings also revealed a significant difference (p<0.05) in the severity of banana diseases across various cultivars from the three counties. The AMF treated bananas showed a significant difference (p<0.05) in plant height, total leaf area and chlorosis in comparison to other treatments. The study also revealed a reduction of Fusarium’s pathogenic effects including chlorosis, reduced leaf surface area and eventual necrosis.

References
  1. Aloui, A., Dumas-Gaudot, E., Daher, Z., van Tuinen, D., Aschi-Smit, S., Morandi, D. (2012): Influence of arbuscular mycorrhizal colonisation on cadmium induced Medicago truncatula root isoflavonoid accumulation. Plant physiology and biochemistry, 60, 233-239. https://doi.org/10.1016/j.plaphy.2012.08.014
  2. Aung, K., Jiang, Y., He, S. Y. (2018): The role of water in plant–microbe interactions. The Plant Journal, 93(4), 771-780. https://doi.org/10.1111/tpj.13795
  3. Beneduzi, A., Ambrosini, A., Passaglia, L. M. (2012): Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genetics and molecular biology, 35, 1044-1051. https://doi.org/10.1590/S1415-47572012000600020
  4. Biruma, M., Pillay, M., Tripathi, L. (2007): Banana Xanthomonas wilt: A review of the disease, management strategies and future research directions. African Journal of Biotechnology. http://dx.doi.org/10.4314/ajb.v6i8.56989.
  5. Blomme, G., Dita, M., Jacobsen, K. S., Pérez Vicente, L., Molina, A., Ocimati, W., Prior, P. (2017): Bacterial diseases of bananas and enset: current state of knowledge and integrated approaches toward sustainable management. Frontiers in plant science, 1290. https://doi.org/10.3389/fpls.2017.01290
  6. Boutaj, H., Meddich, A., Roche, J., Mouzeyar, S., El Modafar, C. (2022): The effects of mycorrhizal fungi on vascular wilt diseases. Crop Protection, 155, 105938. https://doi.org/10.1016/j.cropro.2022.105938
  7. Breen, J., Bellgard, M. (2010): Germin-like proteins (GLPs) in cereal genomes: gene clustering and dynamic roles in plant defense. Functional & Integrative Genomics, 10(4), 463–476. https://doi.org/10.1007/s10142-010-0184-1.
  8. Bubici, G., Kaushal, M., Prigigallo, M. I., Gómez-Lama Cabanás, C., Mercado-Blanco, J. (2019): Biological control agents against Fusarium wilt of banana. Frontiers in microbiology, 10, 616. https://doi.org/10.3389/fmicb.2019.00616
  9. Campos, J. C., Manrique-Silupú, J., Dorneanu, B., Ipanaqué, W., Arellano-García, H. (2022): A smart decision framework for the prediction of thrips incidence in organic banana crops. Ecological Modelling, 473, 110147. https://doi.org/10.1016/j.ecolmodel.2022.110147
  10. Carlier, J., Robert, S., Roussel, V., Chilin-Charles, Y., Lubin-Adjanoh, N., Gilabert, A., Abadie, C. (2021). Central American and Caribbean population history of the Pseudocercospora fijiensis fungus responsible for the latest worldwide pandemics on banana. Fungal Genetics and Biology, 148, 103528. https://doi.org/10.1016/j.fgb.2021.103528
  11. Caron, M., Fortin, J. A., Richard, C. (1986): Influence of substrate on the interaction of Glomus intraradices and Fusarium oxysporum f.sp. radicis-lycopersici on tomatoes. Plant and Soil, 87, 233-239. https://doi.org/10.1007/BF02181862
  12. Chowdhury, S., Basu, A., Kundu, S. (2017): Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame. Frontiers in Plant Science, 8. https://doi.org/10.3389/fpls.2017.00410.
  13. Chu, X. T., Fu, J. J., Sun, Y. F., Xu, Y. M., Miao, Y. J., Xu, Y. F., Hu, T. M. (2016): Effect of arbuscular mycorrhizal fungi inoculation on cold stress-induced oxidative damage in leaves of Elymus nutans Griseb. South African Journal of Botany, 104, 21-29. https://doi.org/10.1016/j.sajb.2015.10.001
  14. Declerck, S., Plenchette, C., Strullu, D. G. (1995): Mycorrhizal dependency of banana (Musa acuminata, AAA group) cultivar. Plant and Soil, 176, 183-187. https://doi.org/10.1007/BF00017688
  15. Declerck, S., Risède, J. M., Rufyikiri, G., Delvaux, B. (2002): Effects of arbuscular mycorrhizal fungi on severity of root rot of bananas caused by Cylindrocladium spathiphylli. Plant Pathology, 51(1), 109-115. https://doi.org/10.1046/j.0032-0862.2001.656.x
  16. Deltour, P., França, S. C., Pereira, O. L., Cardoso, I., De Neve, S., Debode, J., Höfte, M. (2017): Disease suppressiveness to Fusarium wilt of banana in an agroforestry system: influence of soil characteristics and plant community. Agriculture, ecosystems & environment, 239, 173-181. https://doi.org/10.1016/j.agee.2017.01.018
  17. Dita, M., Barquero, M., Heck, D., Mizubuti, E. S., Staver, C. P. (2018): Fusarium wilt of banana: current knowledge on epidemiology and research needs toward sustainable disease management. Frontiers in plant science, 9, 1468. https://doi.org/10.3389/fpls.2018.01468
  18. Durán, P., Viscardi, S., Acuña, J. J., Cornejo, P., Azcón, R., Mora, M. D. L. L. (2018): Endophytic selenobacteria and arbuscular mycorrhizal fungus for Selenium biofortification and Gaeumannomyces graminis biocontrol. Journal of soil science and plant nutrition, 18(4), 1021-1035. http://dx.doi.org/10.4067/S0718-95162018005002902
  19. Ebimieowei, E., Wabiye, Y. H. (2011): Control of black Sigatoka disease: Challenges and prospects. African Journal of Agricultural Research, 6(3), 508-514. https://doi.10.5897/AJAR10.223
  20. Elsen, A., Van der Veken, L., De Waele, D. (2007): AMF-Induced bioprotection against migratory plant-parasitic nematodes in banana. In III International Symposium on Banana: ISHS-ProMusa Symposium on Recent Advances in Banana Crop Protection for Sustainable 828 (91-100). https://doi.org/10.17660/ActaHortic.2009.828.8
  21. FAO (2017): Food and Agriculture Organization. 2017. FAOSTAT Database. Retrieved from http://faostat3.fao.org/.
  22. FAO (2017): The future of food and agriculture–trends and challenges. Rome: Food and Agriculture Organization of the United Nations; 2017. Retrieved from http://faostat3.fao.org/food/agriculture–trends/challenges.
  23. FAO (2023): EST: Banana facts. Retrieved from https://www.fao.org/economic/est/est-commodities/ oilcrops/bananas/bananafacts/en/#.ZBQtL3ZBzIV.
  24. Fuller, D. Q., Boivin, N., Hoogervorst, T., Allaby, R. (2015): Across the Indian Ocean: the prehistoric movement of plants and animals. Antiquity, 85(328), 544-558. https://doi.org/10.1017/S0003598X00067934
  25. George, M., Cherian, K. A., Mathew, D. (2022): Symptomatology of Sigatoka leaf spot disease in banana landraces and identification of its pathogen as Mycosphaerella eumusae. Journal of the Saudi Society of Agricultural Sciences, 21(4), 278-287. https://doi.org/10.1016/j.jssas.2021.09.004
  26. Gomes, S., Douhan, G. W., Lehner, M. S., Bibiano, J., Mizubuti, E. S. G. (2018): Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil. Plant Pathology, 67(2), 295–302. https://doi.org/10.1111/ppa.12752.
  27. Graham, J. H., Menge, J. A. (1981): Influence of vesicular-arbuscular mycorrhizae and soil-phosphorus on take-all disease of wheat. Phytopathology. 71 (8), 877-877. https://doi.org/0031-949X/82/01009504
  28. Gryndler, M., Šmilauer, P., Püschel, D., Bukovská, P., Hršelová, H., Hujslová, M., Jansa, J. (2018): Appropriate non-mycorrhizal controls in arbuscular mycorrhiza research: a microbiome perspective. Mycorrhiza, 28(5), 435-450. https://doi.org/10.1007/s00572-018-0844-x
  29. Gul, N., Khanzada, A. M., Khanzada, M. A., Rajput, A. Q., Shah, G. S., Sahito, O. M. (2018). Responses of banana cigar end rot pathogen to chemical fungicides. Plant Protection, 2(2), 35-44. https://doi.org/10.33804/pp.003.02.3145
  30. Guo, G., Wang, B., Ma, W., Li, X., Zhu, C., Ming, J., Zeng, H. (2013): Biocontrol of Fusarium wilt of banana: Key influence factors and strategies. African Journal of Microbiology Research, 7(41), 4835-4843. https://doi.org/10.5897/AJMR2012.2392
  31. Harrier, L. A., Watson, C. A. (2004): The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil‐borne pathogens in organic and/or other sustainable farming systems. Pest Management Science: formerly Pesticide Science, 60(2), 149-157. https://doi.org/10.1002/ps.820
  32. Hassan, K. (2010): Postharvest handling of fruits and vegetables. Department of Horticulture, Bangladesh Agricultural University, Mymensingh, 2202, 2-3. Retrieved from https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=640986a73533ca3e4640869e0521c093b43335c6
  33. Henderson, J., Pattemore, J. A., Porchun, S. C., Hayden, H. L., Van Brunschot, S., Grice, K. R. E., Peterson, R. A., Thomas-Hall, S. R., Aitken, E. A. B. (2006): Black Sigatoka disease: new technologies to strengthen eradication strategies in Australia. Australasian Plant Pathology, 35(2), 181. https://doi.org/10.1071/ap06017.
  34. Ilyas, M. B., Ghazanfar, M. U., Khan, M. A., Khan, C. A., Bhatti, M. A. R. (2007): Post harvest losses in apple and banana during transport and storage. Pakistan Journal of Agricultural Sciences, 44(3), 534-539. https://api.semanticscholar.org/CorpusID:86183413
  35. Jaizme-Vega, M. C., Sosa Hernández, B., Hernández Hernán, J. M. (1997): Interaction of arbuscular mycorrhizal fungi and the soil pathogen Fusarium oxysporum f. sp. cubense on the first stages of micropropagated Grande Naine banana. 490, 285-296. https://doi.org/10.17660/ActaHortic.1998.490.28
  36. Jaizme-Vega, M., Rodríguez-Romero, A. S., Hermoso, C. M., Declerck, S. (2003): Growth of micropropagated bananas colonized by root-organ culture produced arbuscular mycorrhizal fungi entrapped in Ca-alginate beads. Plant and Soil, 254, 329-335. https://doi.org/10.1023/A:1025523632413
  37. Kang, I. J., Kim, K. S., Beattie, G. A., Chung, H., Heu, S., Hwang, I. (2021): Characterization of Xanthomonas citri pv. glycines population genetics and virulence in a national survey of bacterial pustule disease in Korea. The Plant Pathology Journal, 37(6), 652. https://doi.org/10.5423/PPJ.FT.11.2021.0164
  38. Kumar, K. S., Bhowmik, D., Duraivel, S., & Umadevi, M. (2012): Traditional and medicinal uses of banana. Journal of Pharmacognosy and Phytochemistry, 1(3), 51-63. https://dx.doi.org/10.22271/phyto-2278-4136
  39. Li, C., Chen, S., Zuo, C., Sun, Q., Ye, Q., Yi, G., Huang, B. (2011): The use of GFP-transformed isolates to study infection of banana with Fusarium oxysporum f. sp. cubense race 4. European Journal of Plant Pathology, 131(2), 327–340. https://doi.org/10.1007/s10658-011-9811-5.
  40. Li, C., Yang, J., Li, W., Sun, J., Peng, M. (2017): Direct Root Penetration and Rhizome Vascular Colonization by Fusarium oxysporum f. sp. cubense are the Key Steps in the Successful Infection of Brazil Cavendish. Plant Disease, 101(12), 2073–2078. https://doi.org/10.1094/pdis-04-17-0467-re.
  41. Linderman, R. G. (2000): Effects of Mycorrhizas on Plant Tolerance to Diseases: Mycorrhiza-disease interactions. Arbuscular mycorrhizas: Physiology and function, 345-365. https://doi.org/10.1007/978-94-017-0776-3_15
  42. Meyer, J. R., Linderman, R. G. (1986): Response of subterranean clover to dual inoculation with vesicular-arbuscular mycorrhizal fungi and a plant growth-promoting bacterium, Pseudomonas putida. Soil biology and biochemistry, 18(2), 185-190. https://doi.org/10.1016/0038-0717(86)90025-8
  43. Mohammed, AA., C. Mak, K.W. Liew, Y.W. Ho. (1999): Early evaluation of banana plants at nursery stage for fusarium wilt tolerance. AGRIS - International System for Agricultural Science and Technology. Retrieved from; https://agris.fao.org/search/en/providers/122430/records/6472377ae17b74d2224ede7
  44. Mohandas, S., Manjula, R., Rawal, R. D., Lakshmikantha, H. C., Chakraborty, S., Ramachandra, Y. L. (2010): Evaluation of arbuscular mycorrhiza and other biocontrol agents in managing Fusarium oxysporum f. sp. cubense infection in banana cv. Neypoovan. Biocontrol Science and Technology, 20(2), 165–181. https://doi.org/10.1080/09583150903438439.
  45. Molla, M. M., Islam, M. N., Nasrin, T. A. A., Salam, M. A., Hoque, M. A. (2012): Survey on postharvest practices and losses of banana in selected areas of Bangladesh. Bangladesh Journal of Agriculture, 37(1), 27-35. https://doi.org/10.3329/bjar.v35i3.6451
  46. Momanyi, K. M., Muthomi, J. W., Kimenju, J. W. (2021): Fusarium Wilt of Banana in Kisii County, Kenya. Current Agriculture Research Journal, 9(1), 16–26. https://doi.org/10.12944/carj.9.1.03.
  47. Morandi, D., Bailey, J. A., Gianinazzi-Pearson, V. (1984): Isoflavonoid accumulation in soybean roots infected with vesicular-arbuscular mycorrhizal fungi. Physiological Plant Pathology, 24(3), 357-364. https://doi.org/10.1016/0048-4059(84)90009-2
  48. Muthee, A. I., Gichimu, B. M., Nthakanio, P. N. (2019): Analysis of banana production practices and constraints in Embu County, Kenya. Asian Journal of Agriculture and Rural Development, 9(1), 123-132. https://doi.org/10.18488/journal.1005/2019.9.1/1005.1.123.132
  49. Mwangi, M., Nakato, V. (2009): Key factors responsible for the Xanthomonas wilt epidemic on banana in East and Central Africa. Acta Horticulturae, 828, 395-404. https://doi.org/10.17660/actahortic.2009.828.41
  50. Nakato, V., Mahuku, G., Coutinho, T. (2017): Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade. Molecular Plant Pathology, 19(3), 525–536. https://doi.org/10.1111/mpp.12578.
  51. Nayak, A. K., Nahar, S., Dinesh, K. (2018): An economic study of post-harvest losses of banana in Durg district of Chhattisgarh. International Research Journal of Agricultural Economics and Statistics, 9(1), 82-89. https://doi.org/10.15740/has/irjaes/9.1/82-89
  52. Ocimati, W., Were, E., Groot, J. C., Tittonell, P., Nakato, G. V., Blomme, G. (2018): Risks posed by intercrops and weeds as alternative hosts to Xanthomonas campestris pv. musacearum in banana fields. Frontiers in plant science, 9, 1471. https://doi.org/10.3389/fpls.2018.01471
  53. Olowe, O. M., Olawuyi, O. J., Sobowale, A. A., Odebode, A. C. (2018): Role of arbuscular mycorrhizal fungi as biocontrol agents against Fusarium verticillioides causing ear rot of Zea mays L. (Maize). Current Plant Biology, 15, 30–37. https://doi.org/10.1016/j.cpb.2018.11.005.
  54. Onyambu, M. M., Onyango, B. O., Muraya, M. M., Ong’au, P. M., Ogolla, F. O. (2021): Prevalence of banana Xanthomonas wilt in Nithi, Tharaka-Nithi County in Kenya. Journal of Bioscience and Agriculture Research, 27(02), 2267–2277. https://doi.org/10.18801/jbar.270221.276.
  55. Ploetz, R. C. (2015): Management of Fusarium wilt of banana: A review with special reference to tropical race 4. Crop Protection, 73, 7–15. https://doi.org/10.1016/j.cropro.2015.01.007.
  56. Ploetz, R. C., Evans, E. A. (2015): The future of global banana production. Horticultural Reviews: 43, 311-352. https://doi.org/10.1002/9781119107781.ch06
  57. Ploetz, R. C., Kema, G. H., Ma, L. J. (2015): Impact of diseases on export and smallholder production of banana. Annual review of phytopathology, 53, 269-288. https://doi.org/10.1146/annurev-phyto-080614-120305
  58. Purwati, R. D., Hidayah, N. (2008): Inoculation methods and conidial densities of Fusarium oxysporum f. sp. cubense in Abaca. HAYATI Journal of Biosciences, 15(1), 1-7. https://doi.org/10.4308/hjb.15.1.1
  59. Rajapaksha, L., Gunathilake, D. C., Pathirana, S. M., Fernando, T. (2021): Reducing post-harvest losses in fruits and vegetables for ensuring food security—Case of Sri Lanka. Food Process Technologies, 9(1), 7-16. https://doi.org/10.15406/mojfpt.2021.09.00255
  60. Raza, W., Yousaf, S., Rajer, F. U. (2016): Plant growth promoting activity of volatile organic compounds produced by biocontrol strains. Science Letters, 4(1), 40-43. https://doi.org/10.1038/slet24856
  61. Rodríguez-Romero, A. S., Guerra, M. S. P., del Carmen Jaizme-Vega, M. (2005): Effect of arbuscular mycorrhizal fungi and rhizobacteria on banana growth and nutrition. Agronomy for sustainable development, 25(3), 395-399. https://doi.org/10.1051/agro:2005039
  62. Rufyikiri, G., Declerck, S., Dufey, J. E., Delvaux, B. (2000): Arbuscular mycorrhizal fungi might alleviate aluminium toxicity in banana plants. New phytologist, 148(2), 343-352. https://doi.org/10.1046/j.1469-8137.2000.00761.x
  63. Saha, C. K., Ahamed, M. K., Hosen, M. S., Nandi, R., Kabir, M. (2021): Post-harvest Losses of Banana in Fresh Produce Marketing Chain in Tangail District of Bangladesh. Journal of the Bangladesh Agricultural University, 19(3), 389-397. https://doi.org/10.5455/jbau.74902
  64. Schouteden, N., De Waele, D., Panis, B., Vos, C. M. (2015): Arbuscular Mycorrhizal Fungi for the Biocontrol of Plant-Parasitic Nematodes: A Review of the Mechanisms Involved. Frontiers in Microbiology, 6. https://doi.org/10.3389/fmicb.2015.01280.
  65. Sharma, A. K., Johri, B. N., Gianinazzi, S. (1992): Vesicular-arbuscular mycorrhizae in relation to plant disease. World journal of microbiology and biotechnology, 8, 559-563. https://doi.org/10.1007/bf01238788
  66. Siamak, S. B., Zheng, S. (2018): Banana Fusarium wilt (Fusarium oxysporum f. sp. cubense) control and resistance, in the context of developing wilt-resistant bananas within sustainable production systems. Horticultural Plant Journal, 4(5), 208-218. https://doi.org/10.1016/j.hpj.2018.08.001
  67. Snedecor, G., Cochran, W. (1989): Statistical Methods, Iowa State University Press, Iowa City, IA, USA, 8th edition. https://doi.org/10.1037/13268-000
  68. Sugianti, C., Imaizumi, T., Thammawong, M., Nakano, K. (2022): Recent Post-harvest Technologies in the Banana Supply Chain. Reviews in Agricultural Science, 10, 123-137. https://doi.org/10.7831/ras.10.0_123
  69. Thangavelu, R., Mustaffa, M. M. (2010): First report of corm rot disease caused by Sclerotium rolfsii in banana. Australasian Plant Disease Notes. https://doi.org/10.1071/dn10012.
  70. Tinzaara, W., Mutambuka, M., Oyesigye, E., Blomme, G., Dita, M., Gold, C. S., Karamura, E. (2021): Banana wilt diseases: current status and future research strategies for their management. International Journal of Pest Management, 1-20. https://doi.org/10.1080/09670874.2021.1992685
  71. Tooker, J. F., Frank, S. D. (2012): Genotypically diverse cultivar mixtures for insect pest management and increased crop yields. Journal of Applied Ecology, 49(5), 974-985. https://doi.org/10.1111/j.1365-2664.2012.02173.x
  72. Tripathi, L., Mwaka, H., Tripathi, J. N., Tushemereirwe, W. K. (2010): Expression of sweet pepper Hrap gene in banana enhances resistance to Xanthomonas campestris pv. musacearum. Molecular Plant Pathology, 11(6), 721-731. https://doi.org/10.1111/j.1364-3703.2010.00639.x
  73. Tripathi, L., Mwangi, M., Abele, S., Aritua, V., Tushemereirwe, W. K., Bandyopadhyay, R. (2009): Xanthomonas Wilt: A Threat to Banana Production in East and Central Africa. Plant Disease, 93(5), 440–451. https://doi.org/10.1094/pdis-93-5-0440.
  74. Ugarte Fajardo, J., Bayona Andrade, O., Criollo Bonilla, R., Cevallos‐Cevallos, J., Mariduena‐Zavala, M., Ochoa Donoso, D., Vicente Villardon, J. L. (2020): Early detection of black Sigatoka in banana leaves using hyperspectral images. Applications in plant sciences, 8(8), e11383. https://doi.org/10.1002/aps3.11383
  75. Uwamahoro, F., Berlin, A., Bylund, H., Bucagu, C., Yuen, J. (2019): Management strategies for banana Xanthomonas wilt in Rwanda include mixing indigenous and improved cultivars. Agronomy for Sustainable Development, 39, 1-11. https://doi.org/10.1007/s13593-019-0569-z
  76. Viljoen, A., Mostert, D., Chiconela, T., Beukes, I., Fraser, C., Dwyer, J., Molina, A. B. (2020): Occurrence and spread of the banana fungus Fusarium oxysporum f. sp. cubense TR4 in Mozambique. South African Journal of Science, 116(11-12), 1-11. https://doi.org/10.17159/sajs.2020/8608
  77. Wachira, P., Mwaniki, S. W., Ndiritu, M. M., Kimenju, J., Gathaara, V. (2013): Incidence of pests and diseases affecting banana in a commercial banana production setting in Kenya. https://www.semanticscholar.org/paper/incidence-of-pests-and-diseases-affecting-banana-in-WachiraMwaniki/36034b127b86 33a3c13bce85f2b7660b46da2f6f.
  78. Wahome, C. N., Maingi, J. M., Ombori, O., Kimiti, J. M., Njeru, E. M. (2021): Banana production trends, cultivar diversity, and tissue culture technologies uptake in Kenya. International Journal of Agronomy, 2021, 1–11. https://doi.org/10.1155/2021/6634046
  79. Waweru, B. W., Losenge, T., Kahangi, E. M., Dubois, T., Coyne, D. (2013): Potential biological control of lesion nematodes on banana using Kenyan strains of endophytic Fusarium oxysporum. Nematology, 15(1), 101-107. https://doi.org/10.1163/156854112x645606
  80. Weng, W., Yan, J., Zhou, M., Yao, X., Gao, A., Ma, C., Cheng, J., Ruan, J. (2022): Roles of Arbuscular mycorrhizal fungi as a biocontrol agent in the control of plant diseases. Microorganisms, 10(7), 1266. https://doi.org/10.3390/microorganisms10071266.
  81. Widijanto, H., Muliawati, E. S., Favreta, V. F., Hidayanto, M. (2021): The Growth Response of Banana cv. Barangan (Musa acuminata L.) from Tissue Culture with Organic Manure and Arbuscular Mycorrhizal Fungi. Indian Journal of Agricultural Research, 55(5), 597-602. https://doi.org/10.18805/ijare.a-633
  82. Xue, L., Cui, H., Buer, B., Vijayakumar, V., Delaux, P. M., Junkermann, S., Bucher, M. (2015): Network of GRAS transcription factors involved in the control of arbuscule development in Lotus japonicus. Plant Physiology, 167(3), 854-871. https://doi.org/10.1104/pp.114.255430
  83. Youssef, K., Mustafa, Z. M. M., Kamel, M. A. M., Mounir, G. A. (2020): Cigar end rot of banana caused by Musicillium theobromae and its control in Egypt. Archives of Phytopathology and Plant Protection, 53(3-4), 162–177. https://doi.org/10.1080/03235408.2020.1735139.