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Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
75-79.Views:254Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.
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Tree growth and inner characteristics of fruits in pear cultivars
29-32.Views:238Summary The aim of study was to investigate growth (crown growth and trunk diameter) and fruit characteristics [dry matter content (%), pectin (%), total acid (%) contents] of 10 pear cultivars ('Móri császár', 'Nyári Kálmán', 'Mogyoródi óriás', 'Fehérvári körte', 'Szegfa körte', 'Piroska', 'Mézes körte'). The crown development of the cv. 'Fehérvári körte' can be regarded as outstanding among the examined cultivars. The growth of the cv. 'Mogyoródi óriás' was low, this cultivar showed the poorest growth vigour. The width of the crown in several cultivars developed at a similar rate as the height of the crown. Outstanding values were shown by cvs. 'Fehervári körte' and 'Móri császár'. The development of trunk diameter was the highest for cvs. 'Piroska' and 'Hóka', and large growth of trunk diameter can be seen on cv. 'Fehervári körte'. Cvs. 'Mogyoródi óriás' and 'Mezes körte'showed weak growth. Outstanding dry matter content of fruits was measured on cvs. 'Fehervári körte' and 'Mézes körte'. The total acid content of fruits of cvs. 'Mézes körte' and 'Fehérvári körte'' was significantly different from the total acid content of cv. 'Mogyoródi óriás'. Pectin content was low in fruits of cvs. 'Mogyoródi óriás' and 'Fehérvári körte', while cv. 'Mézes körte' contained significantly more pectin. Vitamin C content we found was rather high in cvs. 'Mézes körte' and 'Fehérvári körte'.
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Identification of plant taxons by isoelectric focusing
65-67.Views:458Differences were demonstrated in esterasei coenzyme pattern of some essential oil producing plants belonging to the Apiaceae family — fennel (Foeniculum vulgare Mill.), angelica (Angelica archangelica L.), lovage (Levisticum officinale Koch.), dill (Anethum graveolens L.), coriander (Coriandrum sativum L.), anise (Pimpinella anisum L.), caraway (Carum carvi L.) — as well as differences between two varieties of fennel seed by using isoelectric focusing. That method provides quality control in essential oil plants and is suitable to describe isoenzyme pattern characteristic for taxons.
Based on our findings, isoelectric focusing seems to be suitable for identification and differentiation of different plant samples, providing an easy tool for further processing as well as for breeding.
Our further aim is to apply that method to differentiate among samples belonging to the same species according to their value of inner content.
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Preliminary data on attractiveness of phenylacetaldehyde-based lures on economically important plant bug pests (Hemiptera: Miridae)
87-94Views:739Several plant bug species (Miridae) are important pests of crops and vegetables, thus monitoring them is of essential importance for effective pest control. During the current, preliminary study synthetic plant volatile combinations were tested in field conditions in Hungary in alfalfa fields. Beside semiochemical baited traps, sweep-netting was also performed. In the experiments three plant bug species were found in higher numbers: Adelphocoris lineolatus, Lygus rugulipennis and L. pratensis. As a novel, interesting finding L. pratensis was attracted to phenylacetaldehyde baited traps. For all species, both males and females were trapped in all combinations. Sweep-netting and semiochemical baited traps showed different efficacy in case of the three species, as sweep-netting catches were highly biased for A. lineolatus, which indicates the higher efficacy of this method as compared to the tested semiochemical-baited traps. On the other hand, semiochemical baited showed better performance for L. rugulipennis and L. pratensis. For these species none of the tested combinations performed better than phenylacetaldehyde baited traps. The potential implication of results in view of monitoring are discussed.
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Effects of different cytokinins on the shoot regeneration from apple leaves of 'Royal Gala' and 'M.26'
69-75.Views:448The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron, or 6-benzylaminopurine, or meta-topolin, or zeatin, or kinetin, or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg 1-1. Effects of 'these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN). Organogenetic index (0I) calculated from these data was used for the evaluation of efficacy of cytokinins. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, 01: 7.5) was achieved in `Royal Gala' by using TDZ in concentration of 0.5 mg 1-1 (2.271,1M). There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins, namely N9-ribosides applied in less concentration than nonribosides have the same or best regeneration effects except for 6-benzylaminopurine riboside. However, similar relationship could not be detected in the case of 'M.26'. SN was the highest (3.22) using 6.5 mg 1-1 (18.2011M) 6-benzylaminopurine riboside or 8.0 mg 1-1 (21.44 µM) meta-topolin riboside (3.18). SN was not significantly lower (3.12) by using 2.0 mg 1-1 (9.08 1M) TDZ, however, OI was about half as big (0.63 compared to 1.29 or 1.74 with 6-benzylaminopurine riboside or meta-topolin riboside, respectively). 'Royal Gala' had higher organogenetic ability, than `M.26': 3.5-fold higher shoot number per explant and more than 4-fold higher organogenetic index was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26' and if explants of 'Royal Gala' were further cultured with 3 weeks, SN increased from 11.08 to 24.42 on TDZ-containing regeneration medium, which might suggest higher organogenetic ability, too.
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The role and current state of gene reservation of medicinal and aromatic plants in Hungary an overview
19-21.Views:381As it well known the decrease of biodiversity is a large problem all over the world. In case of medicinal plants, where the huge majority of drugs are collected from natural ecosystems, the sustainable, utilization of the populations and reservation of their gene-pools has an increased interest.
In Hungary, the major background of 'in situ' reservation of medicinal plant species, their natural plant associations and ecosystems is the official protection by law. Successful examples are known for the controlled utilization of medicinal and aromatic plant species grown in protected areas. Assuring the naturally occuring high degree of biological and chemical diversity of species is a special task in this field: only maintenance of valuable intraspecific races can form the real genetic basis of natural biologically active compounds.
Maintenance of chemotaxonomical gardens and gene bank collections (seed banks, tissue banks) as 'ex situ' methods of reservation is carried out on an extended range in Hungary. As the required information on storage and maintenance of many medicinal and aromatic plant species is yet missing, exhaustive research is carried out at both genebanks in Hungary, which are specialized for medicinal plant reservation (RIMAP- Budakalász, SZIU- Budapest).
Beside the static conservation methods, 'quasi-production systems' are intended to assure an up-to-date and economically possible way of dynamic reservation with sustainable utilization.
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Irrigation management of a peach orchard
19-24.Views:886The research field was at Siófok, in Hungary, which is situated in the South East side of Lake Balaton. The physical characteristic of the soil is sandy loam and loam and the peach orchard is irrigated. Mainly Sweet Lady (early ripening), Red Heaven (medium ripening) and Weinberger (early ripening) species were installed. In order to achieve the optimal developement level of trees and maximal yield amount and fruit diameter (Sweet Lady 60–75 mm, Red Heaven 60–70 mm, Veinberger 50–60 mm) continous water and nutrient supply is required. The irrigation modeling was set by CROPWAT 8.0 based on the climatic, crop and soil data inputs of the last 10 years. Based on the results, large amount of water is needed for optimal growth of fruit trees, particularly in the summer months, in case of active ground cover (+) and bare soil (–) as well. The irrigation requirement of a tree was found maximum 4 l/hour in certain cases. This irrigation intensity can be achieved – calculated with 12-hour operating time – by using continuous water NAAN Tif drip tube with 2 l/h flux on 3 atm pressure with 16 mm pipe diameter. If lower irrigation intensity is required irrigation can be controlled by the decreased the operation time.
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Study on the micronutrient content of soil and leaf of an organic apple orchard in Eastern Hungary
7-11.Views:784The aim of our study was to investigate the relationship between micronutrient content of soil and leaf in an organic apple orchard. The experiment was carried out at the Experimental Site of the University of Debrecen, Centre of Agricultural Sciences at Pallag in an apple cultivar collection in Eastern Hungary in 2002-2004. The soil sampling was carried out twice per year, at the beginning and the end of the vegetation period in 2002, 2003 and 2004, respectively. The micronutrient content of leaf of seven apple cultivars (Jonagold, Mutsu, Idared, Red Elstar, Egri piros, Reka, Remo) was studied according to phenological phases of apple (April — September) to investigate the dynamism of plant nutrient uptake.
It was found that results of leaf analysis correlated with the obtained results of soil. According to the data of July, manganese and copper content of leaf is in the 'favourable' but zinc is the 'low' range of nutrient supply category. Moreover, there were significant differences in the micronutrient content of leaf among cultivars. From our results it is evident that more reliable data were obtained for fruit nutrition by determining of micronutrient content of soil and plant and calculate their ratios as well.
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Evaluation of supercritical plant extracts on volatile and non volatile biologically active lipophil components
78-83.Views:262Authors dealt more than ten years with the analysis of supercritical extracts. For extraction (SFE) carbon dioxide was used as supercritical solvent. Fractionation of extracts was carried out by releasing the separations pressure at two stages. The extracts were collected as separate samples successively in time.
The traditional extractions were carried out with steam distillation or by using n-hexane and ethanol in Soxhlet apparatus. For the analysis of volatile compounds GC, GC-MS; of non volatile compounds TLC-densitometry and spectroscopic methods were used.
The following general characteristics were established comparing the composition of steam distillated oils with that of volatile SFE fractions. The SFE fractions were richer in monoterpene-esters and poorer in alcohols than the essential oils prepared by traditional way (clary sage, lavandel). Regarding the distributi,n of the monoterpene and sesquiterpene compounds, the SFE fractions contained sesquiterpene hydrocarbon in higher percentage than the distillated oils (e.g. 13-caryophyllene in Salvia fruticosa, (3-caryophyllene, ymuurolene, y-cadinene in Ochnum basilicum). Further the proportion of sesquiterpenes increased in SFE fractions collected successively in time.Significant difference was remarkable in respect of the optical rotationability of lovage oil and SFE fraction which was probably caused by the different ratio between the two ligustilid enantiomers. It was verified in some cases that a part of mono- and sesquiterpenes were present originally in a bounded form (glycosides) in plants. Therefore they appeared in essential oil fractions only after previous acidic treatment (Thymus, Origanum species). During the supercritical extraction the azulenogene sesquiterpene lactones did not transform to azulenes (in chamomile, yarrow), but the non volatile SFE fractions of some Asteraceae plant contained sesquiterpene--lacton of unchanged structure in high quantity (e.g. cnicin in blessed thistle, parthenolide in feverfew). Authors obtained also SFE fractions which were rich in triterpenoids and phytosterols (marigold, common dandelion).
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New sweet cherry cultivars in intensive plantings
13-16.Views:963The study took place in the largest sweet cherry plantation in West Hungary. The purpose has been the identification of those varieties, which will be suitable for intense cultivation, early fruiting and excellent fruit quality, moreover, the selection of the optimal phytotechnical procedures. At the same time, scion-rootstock combinations have been tested also from the point of view of growing intensity and fruiting in high-density plantation. The dense planting is induced to start fruiting early and yield regularly by special methods.Yielding was stimulated by maintaining the balance of vegetative-generative growth by binding the shoots, by summer pruning, by cuts on the trunk and root pruning. Best experiences have been found in yield and quality in the following varieties: Canada Giant, Carmen, Firm Red, Giant Red, Katalin, Kordia, Regina. Dense planting has been feasible also on vigorous rootstock, like P. mahaleb. Dwarfing rootstocks like P-HL-A, Gisela 6, accelerate the formation of flower buds and yielding earlier with fruits of adequate size. ‘Firm Red’ and ‘Giant Red’ excelled with their large fruit (>27 mm diameter) in all combinations, thus being promising under Hungarian conditions.
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Microsatellite based identification of grapevine cultivars traditional in Hungary and in the Carpathian Basin
71-73.Views:306Grapevine cultivars and clones traditional in Hungary and in the Carpathian Basin are maintained in the genetic collection of the Corvinus University of Budapest. Mostly ancient varieties and clones were genotyped using microsatellite loci. The investigated 6 loci were sufficient to distinguish all cultivars. Some clones could also be separated based mostly on the variable VVS2 loci. For 17 out of the 31 investigated cultivars this is the first report on characterization of the polymorphism of the allele lengths by microsatellite markers on loci: VVS2, VVMD7, VVMD27, vrZAG62.
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Old Hungarian grapevine cultivars and their relations characterized with microsatellite markers
27-31.Views:508Thirty-one old Hungarian grapevine (Vitis vinifera L.) cultivars were investigated on 7 microsatellite loci to characterize them, to separate the cultivars from synonym names, and to confirm parent-offspring connections. Conculta (group of cultivars or bud sports) members, such as `Goher' and Tajor' representatives, were studied to find a suitable locus for the separation. Synonyms, conculta members, subcultivars and clones of Turmine, which was the most important cultivar of Tokaj, were also analyzed to separate the members of the different taxonomic levels. Pedigree of 'Kiralyleanyka' was examined to find the missing ancestor, because the parent-offspring connection between the natural hybrid and `Koverszolo' is questionable.
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Irrigation modeling in a pear orchard
75-79.Views:875The pear has large water requirement, therefore the planting of high density and grass covered pear orchards are needed irrigation
conditions in Hungary. Drip irrigation spread in the orchards is due to the 90–95% of water use efficiency. One of the key role of irrigation is the
proper determination of evapotranspiration and crop coefficients. As there is a considerable lack of information for different crops or fruits the
Penman-Monteith method is used for the estimation of evapotranspiration, using CROPWAT 8.0. The research field was the genetic collection of
pear at Újfehértó, in Hungary, which is situated in Nyírség meso-region. Our aim was to establish drip irrigation at this site. Based on the results
of CROPWAT irrigation model the mean amount of the total gross irrigation is between 230–270 mm, within 3 irrigation interval regarding
climatic and rainfall data of the last 10 years. In 2009, due to heavy drought, the total gross irrigation was 355,4mm/year on sandy soil calculating
with 45% total available water depletion in 5 irrigation interval. The sizing of the irrigation system was set to the maximum 0.55 l/s/ha, which is
6.3 l/tree/h. 6.3 l/tree/h can be carried out with a drip emitter having 16 mm wing lines diameter, 4 l/h water flow at 3 atm pressure. -
Optimization of RNA isolation from stone fruits at different ripening stages
101-104.Views:483This study was conducted to select the most appropriate RNA isolation method that can be used successfully in case of stone fruits. The changing pattern of gene expression during the ripening process of stone fruits may elucidate the molecular background of several phenotypical or phytochemical alterations present among different genotypes. Our laboratory aims to study the expression of genes encoding for enzymes that catalyze crucial steps in the flavonoid biosynthesis pathway. RNA isolation from fruit mesocarp is a challanging task due to high levels of sugars and polyphenolics accumulating during fruit development. Therefore, at first, the optimal techniques eligible for RNA isolation from fruit tissues at different ripening stages must be selected. Our study compares three different RNA isolation protocols and describes their potential applicability according to different fruit species and ripening stages.
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Economics of apple-storage I: Comparative time series analysis of apple producer prices in Germany and Hungary
75-79.Views:454Based on standard econometric methods the article analyses the time series of fresh apple producer prices in domestic markets of Germany and Hungary. In Germany, as a consequence of high storage capacities the quantity offered in different parts of the season is relatively stable, that’s why only a rather limited price increasing can be detected. In Hungary, as a consequence of the limited storage capacity this fluctuation is much more important. The modern methods of time series analysis (ARIMA models, stepwise regression) can be efficiently applied for forecasting of price movements.
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Sensory analysis as a supporting method for marjoram breeding
73-77.Views:320Marjoram is one of the most important spices in Hungary, which is utilized both in phytotherapy as well as in alimentary industry. Organoleptic quality of the drugs of two Hungarian varieties of Majorana hortensis /'Francia' and 'Magyar'/ were investigated by two different methods of sensory analysis. The chemical composition of the material was checked by GC analysis of the distilled essential oil. Although their main compounds and proportions showed similar patterns, both the human and instrumental sensory tests based on the complex odour and aroma of the drug, proved a significant differentiation of the two varieties.
On the basis of our results, sensory evaluation may be a useful tool in the practice in determination of complex aromatic values of marjoram, as a spice. At the same time it seems to be a suitable method in promotion of breeding efforts.
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Evaluation of the growing and fruit bearing characteristics of the ‘Lapins’ sweet cherry cultivar grafted on rootstocks with different vigor
15-18.Views:707Sweet cherry is currently considered as a fruit with high interests. The amount of the produced yield is well saleable in the world and also in Hungary year by year, moreover often there is a shortage with the high quality fruits. Researches with the sweet cherry production focus on the intensity growing all over the world, namely the realization of producing with small trees ensuring high quality and quantity. In our examinations ’Lapins’ sweet cherry cultivar was evaluated grafted on rootstocks with different vigor (Gisela 5, Gisela 6, Colt) at the University of Debrecen, Pallag Experimental Station. According to our results trees grafted on Colt rootstock can be described with very low yields due to the excessive high vigor. Trees with Gisela 5 and Gisela 6 rootstocks showed excessive high productivity, as size of the fruits did not reach the required values.
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Antioxidant capacity and total polyphenolic content in quince (Cydonia oblonga Mill.) fruit
33-35.Views:969Fruits of twelve quince (Cydonia oblonga Mill.) commercial cultivars and selections were compared in the ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) of intact and peeled fruits. The antioxidant capacity and total phenolic content ranged between 5.99 and 63.10 mmol AA/100 g FW, and 3.92 and 12.83 g GA/100 g FW, respectively. These ranges cover an almost 8-times variation among the tested genotypes in case of antioxidant capacity and also declares two-times variation for total polyphenolic content. Cultivars ‘Aromate’ and ‘Bereczki’ possessed the lowest antioxidant capacity and ‘Román portugál’ and ‘Bereczki’ had the lowest polyphenolic contents. In contrast, cultivars ‘Champion’ and ‘Konstantinápolyi’ possessed the highest antioxidant capacity and ‘Mezôtúri’ and ‘De Husi’ had the highest polyphenolic contents. The Pearson’s coeffi cient was relatively moderate but signifi cant (r=0.51) indicating a weak interdependence between FRAP and TPC values of quince fruits. Our results led us to the conclusion that quince might be considered as a valuable source of antioxidants and polyphenolics.
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Consumer preference for apples: the role of attributes influencing the choice and consumption
37-43.Views:1068Consumers consider good quality fruits to be those that look good, are firm and offer good flavour and nutritive value. Nowadays, consumers are, however, increasingly interested in food qualities which cannot be discovered by looking, tasting or smelling the products but their roles are not yet really cleared up. Therefore the objective of this study was to explore the importance of selected kinds of attributes (taste, size, colour, cultivar, origin and price) in fluencing the choice of apples of customers. In accordance with several authors, fruit qualities (taste, size and colour) seemed to be the major attributics influencing the choice of apple independently of people's age and gender. However, the size of fruit had lower importance with increased age. Generally, females gave higher importance rating for most attributes than did male. The price was getting more important for consumer's choice with age which can be connected with their socioeconomic situation. The origin of fruit and the cultivar did not have important influence on consumer's choice. Authors also investigated the preference of consumers for six selected apple varieties ('Jonagold', 'ldared', 'Royal Gala', 'Golden Reinders', 'Braeburn' and 'Granny Smith'). and pointed out the role of the origin in fruit quality and in choice of apple in the case of 'Granny Smith' cultivar. Apple fruit samples from Austria, Argentina, Chile, Hungary and South Africa were involved in this study. Authors evaluated fruit quality parameters of above cultivars and compared them to consumer preference. Consumer preference usually represented the quality attributes of the fruit well. It is also concluded that degree of liking of apple cultivars varies through gender and age. Children and young consumers preferred 'Royal Gala', 'Granny Smith and 'Braeburn' mostly. In spite of 'Idared' is one of the cultivars grown on the largest area in Hungary, the lowest preference ratings were given for it in both gender categories. Middle-aged consumers (between 25 and 50 years or age) preferred crispy apples with red or blemished skin color ('Royal Gala' and 'Jonagold'). 'Jonagold' and 'Idared' were the most preferred cultivars for the consumers above the age of 50 likely because of their relatively low price, as price plays a significant influencing role in the purchase of these consumers. The relatively expensive and soury 'Granny Smith' appeared not really preferred by this age group. In spite of the significant differences in instrumentally measured fruit quality parameters among 'Granny Smith' fruit samples from different countries, consumers did not give significantly different preference rating scores for those.
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Evaluation of essential oils by gas-chromatography and a new method: "electronic nose"
85-89.Views:275The chemical quality of the essential oils is determined by their composition as well as by the complex aroma features. For the evaluation of odour and aroma, sensory tests are practised, especially for applications such as aromatherapy, food industry or perfumery. In the recent studies we investigated eight Mentha origins (populations of Mentha iperita, M. spicata and M. arvensis) of our genebank collection using a new and effective instrumental sensory evaluation method parallelly with the usual GC analysis.
The results show that the examined mint clones possess different essential oil accumulation levels and special flavour characteristics, too. The GC analysis of the essential oil revealed characteristic differences at both inter- and intraspecific level in the proportion of mentol, menthon, carvon, limonene, menthyl acetate and in two not identified monoterpenes.
In the sensory tests the special complex aroma enables a reliable distinction with a single exception among the populations by the help of the "electronic nose" equipment. The distinction among the samples based on the sensor signals of the instrument — evaluated by multivariate methods — shows a close relation with the detected monoterpene components of the essential oil.
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Incidence of two leaf fungal diseases in two plum training systems
15-17.Views:1158In a two-year study, we aimed to determine the susceptibility of four plum cultivars to two fungal pathogens of plum (Stigmina carpophila and Polystigma rubrum) in two training systems with tree spacings of 4 x 1.5m and 6 x 3m. Results showed that shothole symptoms were not detected on cvs ’Bluefre’ and ’Stanley’ in August, 2016. Disease incidence was above 50% in the case of ’Čačanska lepotica’ in both training systems in 2016. There were no significant difference between the two training systems. Shot hole incidence was lower in the 6 x 3m spacings compared to the 4 x 1.5m spacings on cv ’President’ in 2016. Cultivar ’Čačanska lepotica’ showed the highest incidence of Stigmina carpophila in the 4 x 1.5m spacing in 2017. Disease incidence of Stigmina carpophila was significantly lower in the 6 x 3m spacing compared to the 4 x 1.5m spacing. Shothole incidences on cv ’President’ were similar to the values in 2016 ranging from 40% to 60%. Leaf disease incidence was higher in the 4 x 1.5m spacing compared to the 6 x 3m plot. Low disease incidence (below 10%) was observed on cv ’Stanley’ in 2017 and only in the 4 x 1.5m spacing. There were no visible symptoms of blackhorn dotty in 2016 due to inadequate weather conditions for the Polystigma rubrum fungus. However, all the four cultivars were infected by Polystigma rubrum in 2017. The most susceptible cultivar was cv ’Čačanska lepotica’ with the highest disease incidence in the 4 x 1.5m spacing. Disease incidence of this cultivar was lower in the 6 x 3m spacing which was significantly less than in the 4 x 1.5m spacing. The least susceptible cultivar was ’Bluefre’ and symptoms were observed only in the spacing of 4 x 1.5m. The disease incidence of cv ’President’ was similarly low to cv ’Stanley’ in both spacings.
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Simultaneousness of reproductive organs of sour cherry cultivars adaptable to Iranian climatic condition
25-31.Views:779Observations were made on simultaneousness of pollen shedding and stigma viability ratio, to clarify the phenology of reproductive organs of nine economically important sour cherry (Prunus cerasus L.) cultivars (‘Érdi bôtermô‘, ‘Debreceni bőtermő‘, ‘Csengődi‘, ‘Kántorjánosi 3‘, ‘Újfehértói fürtös‘, ‘Petri‘, ‘Eva‘, ‘Oblascinszka‘, and ‘Pándy 279’). The main aim was investigation the possible influence of environmental cues in timing and development of phenophases and also responses of different cultivars to seasonal cues. Likely effects of climatic changes on phenological development patterns were also considered. Results showed important aspects of the reproductive biology of sour cherry flowers. The amplitude of the phenophase “beginning of blossoming” between cultivars did not exceed 6 days. There is a maximum 3 day difference in the blooming length of different directions in each cultivar. ‘Pándy 279‘ showed high variability when the position of flowers changed on the tree shoots. ‘Újfehértói fürtös‘ was stable in all four directions of the tree. The pollen shedding period and stigma viability ratio were synchronous. Pollen shedding phenomenon simultaneously occurred with the secretary activities of stigmas in all examined cultivars. Distribution of pollen shedding over the secretary activity of stigmas is almost good. In all five cultivars maximum pollen shedding occurred when the temperature was high during the day. In general the results of this experiment and our previous studies clearly demonstrate that the Pándy groups not recommended for plantation in new orchards of Iran.
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Phyl-Gold: a product to diminish russeting of 'Golden Delicious' apples
131-133.Views:294Producing high quality apples in mature trees of the cv. 'Golden Delicious' is rather difficult because of the russeting of it's fruits, especially in seasons of high relative humidity and/or wetness.
Earlier experiments proved the possibility to decrease russeting by treatments of GA4.7 during a period after petal fall.
Phyl-Gold, a product of Phylaxia Co. (ai. 10 g1-1 GA4.7) was applied to inhibit russet formation in fruit skin of 'Golden Delicious' apples. Four consecutive sprays were carried out (with 10 ppm a.i.) in weekly intervals, starting at petal fall and tank-mixed with the current pesticides of the plant protection program.
Due to the GA4.7 application the russet formation decreased at a rate of economical importance. As for the side-effects of treatments, fruit set was reduced slightly in trees treated, however, there was no consequent influence on return bloom.
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Effect of N, P, K and Mg fertilizers on some vegetative and generative parameters of a sweet cherry cultivar
27-30.Views:920This two-year-study was aimed to provide results on the effect of nitrogen, phosphorus, potassium and magnesium fertilizer treatments (control, NP, NPK, NPKMg) on vegetative and generative features of the sweet cherry cultivar ‘Carmen’. Examinations were performed in an orchard planted in 2012 on Prunus mahaleb rootstock with spacing of 5 x 2.5 m. All treatments improved the vegetative features of the sweet cherry trees in both years of 2016 and 2017. Fertilizer treated trees increased trunk cross section area (TCSA) with 51.3-63.1%, while control trees showed 48.3% trunk growth increase. Yields of control trees were lower in both years (5.9-7.2 kg/tree), than that of the fertilized trees (7.8-11.3 kg/tree). Treatments also increased the phosphorus (16-22%), magnesium (12-20%) and potassium content (3.5-18%) of the fruits compared to control treatments.
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:396The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
