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Comparative study of heirloom tomato varieties
31-33.Views:428The study examined the health status, growth type, berry morphology, berry quality, and gastronomical value of 60 heirloom tomato lines. In the middle of June, the level of virus infection was medium in the plant stock according to the assessments. 21% of the lines did not show symptoms of virus infection. During the vegetation period, the major fungal diseases were Alternaria by the middle of August and Phytophthora from the beginning of September. In spite of the fact that no fungicides were applied, 13% of the lines were healthy and 36% showed medium infection at the end of August. Half of the lines had a strong, 23% of the lines had a medium growth vigour. The majority (74%) of the lines showed strong or medium lateral shoot growth and only 7% of them proved to be less prone to branching. In spite of the rapid growth, 20% of the lines had a weak main stem, the majority of the lines had a main stem medium strength. The different shapes occurred in different colours, in addition to the traditional red colour (53%), there were orange, yellow, wine-red (blackish), lilac, green, almost white or multicoloured varieties. Unfortunately, most of the lines were prone to longitudinal or circular cracking of the berries. Based on the first experiences, all tested variety types had more favourable utilization characteristics then the traditional tomatoes. Varieties with a thick flesh and harmonic acid/sugar ratio such as some of the ox-heart varieties are especially valuable. For drying, the elongated types proved to be the best. The hollow tomato is the most special type which can be utilized most diversely. It is especially suitable for making white tomato soup and, “Concasse" due to its easily removable placenta rich in jelly materials and its thick flesh. In addition, it can be used for preparing stuffed tomato.
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Hazardous element content and consumption risk of 9 apricot cultivars
61-65.Views:950The heavy metals pollution is one of the problems that arise due to the increased uses of fertilizers and other chemicals to meet the higher demands of food production for human consumption. In order to assess possible health risk of apricot (Prunus armeniaca L.) consumption, levels of Arsenic, Cadmium, Mercury and Lead were determined in fresh and dried samples of "Jumbo cot", "Tom cot", "Gold strike", "Gold bar", "Bergeron", "Bergarouge", "Sweet cot", "Yellow cot" and "Zebra" apricot cultivars. Wet digestion of samples with concentrate HNO3 – H2O2 digester mixture and inductively coupled plasma–atomic emission spectroscopy was used. Highest content of As, Cd, Hg and Pb among all cultivars, were 0.5, 0.04, 1.5 and 0.5mg/kg of dried apricot samples. Fresh fruit samples also contain 0.2, 0.016, 0.6 and 0.2 mg/kg of Arsenic, Cadmium, Mercury and Lead respectively. Daily intake of metals, hazard quotient and health risk index to reveal health risk possibility of dried and fresh fruits consumption were calculate and compared.
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Model experiments for establishment of in vitro culture by micrografting in apple
47-49.Views:466Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips survived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.
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Adaption of temperate climate horticultural plants in tropical and subtropical developing countries (Review Article)
7-11.Views:712Adaption of temperate climate horticultural plants in tropical and subtropical developing countries (Review Article)
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Investigation of flowering dynamics of the basil (Ocimum basilicum L.) and its consequences in production
74-80.Views:470The flowering dynamics of Ocimum basilicum L., (a common population maintained at the UHFI) was studied with the aim to create an exact and practically applicable method for definition of phenological phases.
According to our observation the development of individual flowers of the basil can be characterized by 8 distinguishable phases, which must be considered for description of the actual phenological stage of a spike.
An accurate model was created for the unambiguous description of flowering process of different flowers within the spike and for the individual plant as a whole. The new flowering index formula is calculated from the number of flowers weighed by their phenological phases.
The time dependence of flowering is presented by functions fitted to values of the flowering index. The results reflected different patterns of the main inflorescence, the inflorescences formed on the side shoots in the first or in the second half of the flowering period. However, for description of the flowering process of the whole plant, a sigmoid function proved to be the appropriate model.
The accumulation process of the essential oil could be characterized by the flowering index values. They showed close correlation (r=0.964) at high probability.
The new method assures an exact definition of phenological phases in basil. Its application seems to be optimal during breeding procedures, seed production and even in production of high quality drugs, according to the requirements of the Good Agricultural Practice (Guidelines for GAP, 1998).
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Changes of the characteristics of Satureja hortensis L. herb during flowering period
56-60.Views:264In this study the changes of the characteristics of Satureja hortensis herb were investigated during flowering period, in case of savories of 4 different origins. The change of drying ratio, leaf/stem ratio, essential oil content, the ratio of several essential oil components (carvacrol, y-terpinene, p-cymol, a-pinene, p-pinene and fi-caryophyllene) were studied from bud formation till seed ripening.
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Specialities of the vegetation start and level of primary fruit set affect fruit quality
17-22.Views:596Thinning is a h ighly crucial point of the apple production technology. According to results of numerous studies the earliest thinning is deemed to have the best amending effects. There can be considerable difference between trees of the same cul tivar and age in a plantation in respect to their flowering, in the numbers of fruits set and also in their canopy volume. Thus it can be crucial -just l ike in the case of pruning- to establish a fruit thinni ng su i ted for specif ic characteristics of actual trees. This experiment was established to examine how does primary fruit set (fruit load before thinning) and further on specific (fruit/TCS cm2) and absolute (fruit/tree) fruit-load of the trees affect quality attri butes at harvest. Our experiment was establ ished in a plantation on medium-tight loamy soil in north-east of Hungary. Harvest date was determined w ith the joint observation of the calendar date, starch-index, flesh firmness, background colour and fruit weight. Three fruit-load levels were established based on local experience and on data of several years, I Ot/ha lower and higher besides the advised optimum yield in the same orchard with slender-spindle shaped 'Gala must' cultivar standing on M9 rootstock in 1m x 3,8 m spacing. In the establishment of the 15, 25 and 35t/ha fruit-load levels on 20-20 apple trees total number of fruits set was counted at each tree. After this number of apples due to be removed was defined using an objective index on the basis of trunk cross sections (fruit/TCScm2) (Lafer, 1999). The following attributes were measured: weight (g), flesh firmness (lb/cm2) total soluble solid content (Brix %) and total titrate-able acid content (g/J).From the data on sugar and acid content quality index (Pomona value) was determined (Thialult, 1970). We could ascertain, that in an orchard, of the same aged but in concern to trunk cross sections somewhat different trees besides the specific index (apple/TCS cm2 the absolute fruit load (fruit/tree) can also be an important data, that has considerable effect on the internal quality. Secondly we could observe, that higher level of fruit load before thinning (primary fruit set) negatively affects quality index of the apples irrespective of the specific fruit load level (fruit/cm2 TCS) set later. Results underlines necessity of the earliest chemical thinn ing.
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The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon
46-49.Views:371Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.
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Evaluation of lifespan values of six tree taxa in city streets
25-27.Views:750The aim of this study was to evaluate lifespan values of trees in tree-row system in the streets of Debrecen in two years (2009 and 2017). Six selected taxa (Pyrus calleryana, Acer tataricum, Sorbus intermedia, Magnolia kobus, Acer platanoides and Crataegus x lavalleei) were estimated for the following lifespan parameters: i) trunk diameter (cm), ii) tree crown size (m), iii) trunk status (in 0-5 grades), iv) tree crown status (in 0-5 grades), and v) estimated tree viability (in 0-5 grades). Our results showed that the largest were achieved for Pyrus calleryana and the lowest for Acer tataricum. The largest tree crown diameters were achieved for Acer platanoides and the lowest Magnolia kobus. The best trunk statuses by 2017 were achieved for Pyrus calleryana and the worst Crataegus x lavalleei. The best tree crown statuses by 2017 were achieved for Pyrus calleryana and the worst for Acer tataricum. The best estimated tree viability status was achieved for two taxa (Pyrus calleryana and Acer platanoides). Overall tree values were 2.73 times higher in 2017 compared to 2009. In summary, this study demonstrated the role of appropriate choice of tree taxa for a tree-row system under city street conditions.
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The Hungarian peach production risks
85-89.Views:319Hungary possesses very good agroecological conditions even in a European comparison. Agriculture and food production is highly important despite its decreasing relative economic significance, since providing high quality and affordable food for the population is a basic social necessity and one of the decisive factors in quality of life. The development of vegetable and fruit production is of great importance both from a healthy nutrition as well as from a comparative advantage (cheap labour, expertise, production traditions, favourable climate) point of view.Vegetable and fruit consumption per capita is lower than in developed countries, which is a decisive indicator of healthy nutrition, but improvement is continuous and desired. Appropriate quality and quantity in production is a requirement to meet customers’ needs in a highly competitive market from one year to the next. Hungary can only be competitive if production results are improved while risks are reduced. Fruit production is a sector with high capital requirements, risk assessment is very important from a production, investment as well as from a national economy point of view. Our examinations will focus on production risk, which is present in the annual fluctuation of average yields, especially as a result of extreme weather factors.
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Susceptibility of sour cherry cultivars to isolates of Monilia laxa (Ehrenbergh) Saccardo et Voglino
83-87.Views:494In this study, the susceptibility of 7 commercially important sour cherry cultivars to Monilinia laxa was studied. Artificial inoculation was made with M. laxa isolates, which were isolated from different woody plants. Artificial inoculation was prepared in the laboratory and in the field. In laboratory, flowers of sour cherries while in the field, the two-year old twigs were inoculated in 2006 and 2007. According to results of stigmata inoculation, there were infection ability differences among the isolates originated from five different stone fruit host. Cultivars could be sorted into two susceptibility groups. In the field, twig inoculation in 2007 was made at blossom period and in 2007 at harvest. Seven sour cherry cultivars were inoculated with 8-day-old mycelial culture of M. laxa originated from sour cherry and almond. The agressivity and pathogenicity of the two isolates were measured by the degree of floem death: Results showed that year and phenological stage considerably influenced the degree of symptoms caused by the fungus. After artificial inoculation, tissue death progression was studied by fluorescent microscope. According to results, sour cherry cultivars were sorted into disease susceptibility groups. Susceptibility orders were identical to results on stigmata inoculation.
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New in vitro micrografting method for apple by sticking
79-83.Views:529The requirements for in vitro micrografting in apple are described. In vitro multiplicated shoots of cv. Royal Gala were the sources of rootstocks and scions after different pre-treatment, respectively. Oxidative browning of cut surfaces could be inhibited by the use of antioxidant mixture during grafting process. Scion base cut in v-shape was stuck by 1% agar-agar solution into the vertical slit of rootstock. There was no any displacement and the rate of fused and further developed grafts was 95 percent. Agar-agar between the rootstock and scion made the transport of different materials possible and hold the graft units together until the fusion took place. Fusion was proved also by histological studies. Some of in vitro micrografts were planted and acclimatisated and the survival was 100 percent.
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The effect of Ferbanat L nano-fertilizer on the growing of Petunia x grandiflora ’Musica Blue’
107-109.Views:449During our experiment, the effect of Ferbanat L concentrations were examined by Petunia x grandiflora ‘Musica Blue’ production. The leaf and shoot length, number of flower buds, diameter of flowers, and the date of appearing the first buds were measured. The solution of Ferbanat L in 0.1 % concentration was the most effective on shoot length (21.7 cm). Remarkable increase was observed by the other treatment groups as well comparing to the control group (5.6 cm), the plants treated with solution of Ferbanat L 0.2% (16.5 cm) and 0.3% (14.4 cm) had significant effect as well. The nano-fertilizer had not positive effect on the other examined parameters as leaf and flower size, number of flower buds or chlorophyll content. The culture period shortened five days.
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In vitro multiplication and hardening of grapevine plants in aeriated media
15-18.Views:378In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.
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Fire blight in Hungary between 1996 and 2003
67-70.Views:209Shoot blight of pome fruits caused by Erwinia amylovora, i.e. fire blight, is present in numerous countries of Europe. The disease must have entered into Hungary in the middle of the 1990's and it was first noted and, respectively, identified in 1996 (Bacs-Kiskun county). The losses caused by the pathogen appeared — in orchards and scattered sites of production — in four counties, namely Bacs-Kiskun, Baranya, Bekes and Csongrad at the beginning. From June 1996, a process of eliminating infected parts started in the course of a large action performed under the control of the Department of Plant Protection and Agro-Environmental Economy of the Ministry of Agriculture, under the direction of the plant protection inspectors of the then existing Stations of Plant Health and Soil Conservation. The 'operation' against the disease commenced by cutting back out the infected parts of the canopy and, grubbing them out, respectively. As for the spread of the pathogen (1996-1998) it could be observed that the disease entered into Hungary from the south, south-east and then it also spread into the middle part of the country. As a result of adequate official action and efforts as well as of adequate chemical and antibiotic treatments, moreover because of the introduction of more modern technologies of plant cultivation and those of plant protection it can be reported on that the pathogen hardly appears or does not occur at all on the northern, north-western part of the country. The infection also appears mainly on the parts east of the Danube. Cultivars less susceptible or non-susceptible to the disease are planted in recently established orchards what is also a considerable factor in respect of preventing spread of the pathogen.
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The effects of ACS (1-aminocyclopropane-l-carboxylate synthase) gene down regulation on ethylene production and fruit softening in transgenic apple
65-70.Views:242A detailed examination of the production of ethylene and other ripening parameters during storage period has been undertaken in transgenic apple fruits, where the ethylene biosynthesis was inhibited by antisense ACS (l-aminocyclopropane-l-carboxylate synthase) gene. Data indicate down regulation of ethylene production, softening and spoilage in some transgenic lines. In some cases ethylene production was inhibited for over 90 percent, considerable reduction of softening and spoilage was observed probably due to the reduced activity of cell wall degradable enzymes. ACS activity was also monitored during ripening. The fruits of the best transgenic lines could be stored for minimum 4-5 months longer under 5 °C cold room storage conditions and one month longer at normal room temperature. This molecular approach can provide an alternative way to replace the commonly used and costly atmospheric storage of fruits.
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Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation
53-56.Views:286The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.
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Seed certification and EU conform legislative harmonization in Hungary
151-152.Views:201Seed certification and EU conform legislative harmonization in Hungary
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Work quality assessment of a cherry sorting machine
57-62.Views:976The conditions of the cultivation of marketable cherries are diverse. Choosing the production site on the basis of the climatic conditions, selecting the most appropriate variety taking into account the region and the purpose of the production, utilization of the optimal production method, to ensure the required water and nutrients supply, frost and hail protection techniques, modern technology in crop protection, and professional performance of harvesting and handling, to name just a few of the most important issues. The objective of present study is to determine the qualitative characteristics of the UNITEC cherry sorting machine.
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Epiphytic microbiota of apple in integrated and organic growing
85-89.Views:739The surface microbial contamination is of great interest in case of fruits, since they are they potential sources of spoilage or foodborne diseases. The aim of this work was to compare the epiphytic microbiota of food safety concern of different apple cultivars as a function of cultivation methods (organic versus integrated), production year and place. Investigating 47 samples it was found that the average microbial contamination of the apple surfaces was within a certain range,, practically independent of cultivar, growing area and year. The frequency distribution of the surface bacterial, mould and yeast counts did not show significant differences between growing technologies or varieties. The principal component analysis the samples ranked the apple samples into seven groups on the basis of their microbial contamination level, and the discriminant analysis proved the goodness of grouping. The grouping was independent from cultivars and growing methods. No foodborne pathogen Salmonella spp. and Listeria spp. were found on the surfaces of apples.
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Effects of irregular cropping on nutrient uptake and status of apple trees
41-44.Views:816Produce of harmonic nutrient balance and status of trees is basic task of growers for qualified fruit growing. The role of crop loaded is essential to grow the productivity of trees without nutrient disorder or other nutritional problems. To reduce the frequency of biennial bearing there are some ways (site and species selection, crop regulation, irrigation, nutrition etc.) from them one is the best easy way the proper site and species oriented nutrition. Sometimes the responsible for irregular cropping is the insufficient fertilization. Observed irregular cropping at two apple cultivars (Malus domestica Borkh., cv. ‘Summerred’ and cv. ‘Mutsu’) in an integrated apple orchard at Hajdúnánás-Tedej, in 2007 resulted a chance to determine nutritional status of “bearing” and “non bearing” trees and investigate the effects of irregular cropping on nutrient uptake and possibilities of correction. Our results pointed out that irregularity is connection on nutrition status of trees. Irregular cropping results in insufficient nutrient uptake and disharmony of nutrients. For this reason, the establishment of nutrient status of “non bearing” trees is could permit a chance to correct and improve the nutrient supply of trees which help to breakdown the vicious circle of irregularity.
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The role of insurance in the damager mitigation of fruit orchards
117-122.Views:300Hungary faced many natural disasters in 2007. Due to the estimation of the Hungarian Chamber of Agriculture, the amount of natural disasters that occurred in Spring 2007 (frost, hailstorm and drought in April and May) is about 100 billion HUF. Frost and drought caused damage on about 250 thousand hectares of arable land. Currently, there are four insurance companies dealing with agricultural insurances in Hungary. Their income was nearly 20 billion HUF in 2003, whereas it barely exceeded 6.6 billion HUF in 2004. The reason behind the significant decrease of insurance fee is the state provision made in 2004 stopping fee subsidisation which originally started in 1996, enabling farmers to require a 25-30% reimbursement of the amount paid for insurance. Launching a state subsidisation again would greatly increase the number of agricultural insurances. The law about the national agricultural mitigation system passed last Autumn. This provision declares that the mitigation of agricultural damage that cannot be insured on a business-like basis is based on the common risk-taking by the state and the farmers. The introduction of this system is explained by the fact that the mitigation of damages through disaster can only be carried out if those affected also take part in it, according to EU rules.
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Volatile constituents of Nepeta cataria L., N. glechoma Benth. and N. parviflora M. Bieb. from Hungary
47-50.Views:237In the temperate zone live about 150 species of the Nepeta genus. Our investigations covered the examinations of the volatile oil containing species of the genus endemic in Hungary, Nepeta cataria and Nepeta parviflora. Latter is a relict of the ancient steppe-flora and endemic in Hungary as well. Phytochemical examination of the volatile oil containing plant material has also been carried out. Catnip growing in the Botanical Garden of PTE Department of Botany contained 0,67% volatile oil in May and 0,14% in November. Chemical character of the volatile oils were measured by gas chromatography/mass spectrometry and citronellol, citral-A, citral-B and geraniol components were identified. The composition of the oil of November samples shifted towards citronellol (65%). In both samples insecticide and repellent activity bearing compounds (+)-cis-p-menthane-3,8-diol, and (—)-trans-p-menthane-3,8-diol in 2-2.5 and 4-4.5% amount have been found. The catnip sample deriving from Germany contained a small amount of anetol, citronellol, neral, geraniol and geranial (6-13%), and possibly two isomers of nepetalactone in 23-31%.
The Nepeta parviflora endemic in the Nagyvolgy valley near Nagykaracsony consisted of the same compounds in the investigated years (1998-2000). Its limonene, methyl chavicol, b-cariophyllene, b-selinene, b-cubebene, davanone, germacrene-D constituents have been identified. In the year 2000 different GC % of these compounds were detected in the different organs of the plants.
The closely related species Nepeta cataria var. citriodora contained 83% citral, and the N. glechoma (= Glechoma hederacea) contained 41% a-cubebene, 20% patchoulenol, 7,7% spathulenol respectively. These compounds were identified by gas chromatography and gas chromatography / mass spectrometry.
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Seasonal root development of Cabernet sauvignon grafted on different rootstocks
61-63.Views:342The minirhizotron system gives opportunity to study the root development without disturbing the soil and root. We have found certified differences in root development during the year 2013 among the rootstocks grafted on ‘Cabernet sauvignon’. The number of roots varied according to the rootstocks in different depth of soil layers and also varied the development of ripeness of the root system. We conclude that root system development is affected by soil physical and chemical properties, but differences according to the rootstock genotype on the similar type of soil exist.
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The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'
77-83.Views:362The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.
