Vol 11 No 1 (2005)
Cikkek

Model experiments for establishment of in vitro culture by micrografting in apple

Published March 14, 2005
J. Dobránszky
Research Centre of University of Debrecen, H-4401 Nyíregyháza, P.O.Box 12., Hungary
E. Jámbor-Benczúr
Corvinus University, Department of Floriculture and Dendrology, H-1118 Budapest, Villányi út 35-43 ., Hungary
I. Hudák
Research Centre of University of Debrecen, H-4401 Nyíregyháza, P.O.Box 12., Hungary
K. Magyar-Tábori
Research Centre of University of Debrecen, H-4401 Nyíregyháza, P.O.Box 12., Hungary
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How to Cite

APA

Dobránszky, J., Jámbor-Benczúr, E., Hudák, I., & Magyar-Tábori, K. (2005). Model experiments for establishment of in vitro culture by micrografting in apple. International Journal of Horticultural Science, 11(1), 47-49. https://doi.org/10.31421/IJHS/11/1/557

Abstract

Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips survived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.

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