Published March 14, 2005
The knowledge of tissue culture deserves attention in respect of understanding the development of universal biology. This study intends to contribute to the past of the plant tissue culture by such data of the history of science which have been unprocessed so far. It seems that the life-work of the Hungarian biologist, Dr. Ottó Orsós is a missin...g and essential link between those early plant hormone researchers and the representatives of the pioneers of tissue culture schools who have contributed substantially to the development of the modern in vitro plant morphogenesis and plant cell biology. Orsós cultured kohlrabi tuber cubes on White culture medium in a sterile manner. This way, he could efficiently direct the in vitro morphogenesis of the kohlrabi, the regeneration of its shoot and root, and the formation and steps to subculture of pure callus tissues in 1938. He supported the correctness of its statements by means of detailed anatomical examinations. Orsós successfully rooted and aclimatized complete regenerated plants. We may as well call the above system — in remembrance of the creators of the original concept — "Haberlandt-Orsós model". Between the publishing of his main paper in 1938 and 2003, a period of 65 years has lapsed. On the occasion of this anniversary, we bow before this forgotten pioneer.
The scope of apple growing and marketing has been determined by the economic and political systems in the recent decades. One may follow the booms and collapses in the Hungarian apple industry. Re-establishment of the market economy gives new chances for recovery in the fruit section as well. Reconstruction of the apple industry has started with ...private ownership of lands. In the present work we characterise the still existing apple farms inherited from the previous system, present the newly established orchards in detail, deal with the present state of apple commerce and finally, we try to predict the near future of Hungarian apple industry.
The impact of location, row direction, plant density and rootstock on the sunburn damage of apple cultivars
The effect of row direction (N-S, E-W), plant density (conventional and intensive) and rootstock (M9, MM106 and crab apple) on the sensitivity of 41 apple cultivars to sunburn injury was studied at 6 locations (Derecske, Kálmánáaza, Nagykutas, Nagylapos, Siófok, Tornyospálca). During the observations the cultivars had rootstock-specific prop...erties in respect of sunburn-susceptibility. Accordingly, the injury was decreased in the order: M9, MM106 and seedling rootstocks. The differences in sensitivity depend on the canopy of trees, caused finally by the growing vigour of rootstocks. Accordingly, the highest value of sunburn injury was observed on M9 rootstock, because this rootstock has a dwarfing effect upon the scion cultivars. Thus, vegetative area of these trees grow very slowly and the foliage is not enough compact to protect the fruit from solar radiation. The size and density of the foliage increased in the order: M9, MM 106 and crab apple seedling as rootstock. Moreover, relationships were demonstrated between the diameter of upper part of the crown, the leaf area, the number of fruits per tree and the injury of sunburn. Authors categorized the cultivars in respect of values of sunburn incidence: 1. „Not sensitive", II. „Moderately sensitive" and HI. „Strongly sensitive" categories were constituted. Generally, Topaz and Gala cultivars showed low damage (or were free from symptoms), in contrast to this, Golden mutants suffered relatively much. The most sensitive cultivar was Jonica on all the three rootstocks. We searched for relationship between the fruit quality parameters and the frequency of sunburn. Significant correlation was found in the cases of fruit size and the extent of cover-colour. The latter is interpreted with the fact that the best coloured fruits are found on the periphery of crown as a consequence of more intense irradiation.
Effect of intensity of bee visitation and the foraging behaviour of honeybees on the fruit set and yield of apple (Malus domestica Borkh.)
Based on the results of our experiments, both the relative and the effective intensity of bee visitation were rather different depending on the cultivars as well as the time of the day. While it varied between relatively extreme values (40-80%) in the morning for the varieties examined, there were much smaller differences between the intensity of ...bee visitation at the afternoon, because the relative bee visitation attained 70-90% at each cultivar. These results showed that the differences arising from intensity of bee visitation of different cultivars should be taken into consideration more carefully in the morning in orchard planning and in estimating the number of honeybee colonies required. The results showed that the greatest percentage of fruit set and the highest number of viable seeds per fruit were measured on branches of those cultivars that were most frequently visited by pure pollen gatherer bees as well as by bees collecting both nectar and pollen (mixed behaviour). The effect of pure pollen gatherers and of bees with mixed behaviour was highly significant from the statistical point of view on the fruit set and the number of viable seeds per fruit. Those bees that were sucking nectar only from apple flowers did not proved to be effective pollinators at all. Relationship between their number and the fruit set as well as the number of viable seed per fruit were not significant because the coefficient of correlation was close to nil. The ratio of side worker nectar gatherers was negatively correlated with the fruit set and the seed content of fruits of apple cultivars examined at both of our experimental sites, at Mosonmagyaróvár and Feketeerdő as well. The presence of side worker nectar gatherers resulted in higher decrease of fruit set and seed content of fruits at Feketeerd6 than at Mosonmagyaróvár, especially in the morning. The effect of flower visiting intensity by other pollinating insects was found to be fairly variable according to the time of the day. In the morning they had no effect on the fruit set as well as on the seed content of fruits either at Mosonmagyaróvár or at Feketeerdo. However, at the afternoon, when their intensity was greater, the correlation was a bit stronger.
Effects of aromatic cytokinins on structural characteristics of leaves and their post- effects on subsequent shoot regeneration from in vitro apple leaves of 'Royal Gala'
The effects of different aromatic cytokinins applied in different concentrations and combinations were investigated on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves were studied. Great differences in the anatomical structure of leaves could be detected originating from media contai...ning different types and concentrations of aromatic cytokinins. The number of regenerated shoots per explant and the organogenetic index were used for the evaluation of the post-effect of aromatic cytokinins on shoot regeneration. The histological structure of leaves used for regeneration and their regeneration response showed a good correlation. When the pre-treatment caused a juvenile-like or less-differentiated structure, the number of regenerated shoots per explant increased and often vitrification also decreased and consequently the organogenetic index also increased. A strong interaction between cytokinin-content (type and concentration) of the pre-treatment medium and that of the regeneration medium could also be detected.
Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips sur...vived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.
Some important growing characteristics of apple and apricot cultivars in two fruit growing regions in Romania
The aim of this study was to determine some important growing characteristics of 5 resistant apple (Rajka, Rubinola, Topaz, Otawa and Goldstar) and 7 apricot (NJA 19, Goldrich, Harcot, Venus, Comando, Olimp, Cea mai buna de Ungaria) cultivars in two fruit growing regions in Romania from 2000 to 2003. Height of the apple trees (4-year-old tree) ran...ged between 1.96 m (cv. Topaz) and 2.39 m (cv. Rubinola). Diameter of the crown ranged from 0.96 m (cv. Goldstar) to 1.12 cm (cv. Rajka). The trunk diameter ranged from 3.5 cm (cv. Goldstar) to 5.0 cm (cv. Rajka). The surface of the trunk section was the lowest for cv. Goldstar (9.7 cm') and the largest for cv. Rajka (19.6 cm). Height of the apricot trees (4 years old tree) ranged between 2.69 m (cv. Venus) and 3.38 m (cv. NJA - 19). Diameter of the crown ranged from 2.59 m (cv. Comandor) to 2.77 m (cv. Cea mai buna de Ungaria). The trunk diameter ranged from 9.54 cm (cv. Goldrich) to 13.30 cm (cv. NJA - 19). Length of annual branches was the lowest for cv. Goldrich (45.1 cm) and the highest for cv. NJA - 19 (83.8 cm). Bud swelling of apricot trees began on 8 March for cv. NJA - 19 and ended on 11 March for the control cultivar (Cea mai buna de Ungaria). The blooming started on 16 March for cv. NJA - 19 and 27 Mach for the control cultivar. Duration of fruit growth was 89 days for cv. NJA - 19 and 128 days for cv. Comandor. When the temperature decreased to 1.5 °C (in 2001), percentage of viable pollen grains ranged between 48.86 % (cv. Olimp) and 91.57 % (cv. Venus). The germinating grains ranged from 31 % (cv. Olimp) to 90 % (cv. Harcot). Free pollination was the lowest for cv. NJA - 19 (29 %) and the highest for cv. Harcot (41%), while self-pollination ranged between 6 (cv. Olimp) and 11 % (cvs. Comondor and Harcot). Apple yield ranged from 16.65 t/ha (cv. Otawa) to 24.35 t/ha (cv. Rajka) and the differences varied from 4.45 t/ha to - 3.25 t/ha compared to the control varieties. Apricot yield ranged from 11.47 kg/ tree or 9.53 t/ha (cv. Cea mai buna de Ungaria), to 38.83 kg/tree or 27.34 t/ha (cv. Olimp) and the differences varied from 3 t/ha to 17 t/ha compared to the control varieties. Apple fruit weight ranged from 162 g (cv. Otawa) to 222 g (cv. Goldstar) and apricot fruit weight from 42.52 g (cv. Goldrich) to 68.38 g (cv. Comandor). Color, taste and aroma were very specific to cultivars.
Effect of pruning on disease incidence of apple scab and powdery mildew in integrated and organic apple production
In a three-year study, the effect of two winter pruning treatments (strong and weak) on apple scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) was evaluated on six apple cultivars (`Rewena', `Elstar', 'Liberty', 'Gala', 'Pilot' and `Jonica') in two environmentally-benign apple production systems (integrated and organic). Res...ults on apple scab showed that compared to the integrated production system, the pathogen caused significantly more infection on the leaves of cvs. `Elstar', 'Gala' and `Jonica' in the organic production system. The effect of pruning treatments on apple scab was mainly detectable on the cultivars susceptible to the disease such as cvs. 'Gala' and `Jonica'. The weak pruning correlated with a higher level of apple scab infection than the strong pruning, especially in the organic production system. Primary mildew infection was very low in both protection systems (below 2 %), therefore, effect of pruning on primary mildew infection could not be analysed. Low secondary mildew incidence was detected in the organically treated orchard part; however, incidence of powdery mildew was significantly higher (P> 0.05) on cvs. 'Elstar', 'Pilot' and `Jonica' in the integrated orchard part compared to cvs. 'Gala', 'Rewena' and 'Liberty'. The highest incidence of mildew was on cv. 'Elstar'. Pruning had very low effect on incidence of secondary mildew infection on leaves.
Pollen tube growth in sweet cherry (Prunus avium L.) styles following fully compatible, half compatible and incompatible pollinations
In vivo as well as in vitro pollen tube growth studies along the style were performed, each with two pairs of sweet cherry cultivar combinations by means of fluorescence microscopy. In vivo studies showed that the percentage of pollen tubes penetrating the middle and basal section of the style was higher in the fully compatible 'Margit' x 'Alex' c...ombination than in the half compatible `Germersdorfi 3' x `Alex' cross. The year effect was significant at P=0.] probability level. All pollen tubes in vitro stopped at the upper third of the style in the incompatible 'Vera' x 'Van' cross, whereas in the half compatible 'Alex' x 'Van' 50% of the pollen tubes penetrated to the lower third of the style. By in vitro fluorescence microscopy, it was possible to distinguish half compatible combinations from incompatible ones. Results obtained by in vivo technique only were much ambiguous.
Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis
Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of som...e Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S1S2) and `Harcoe (S1S4) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.
The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings o...f C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification. The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data. Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).
In the present study histology of the leaves of strawberry (Fragaria ananassa Duch.) variety Elsanta was the objective, which has been performed with the beginning of seedling stage, cotyledons, primary leaves and later true leaves, first cataphyll of the runner shoot as well as the bracteoles of the inflorescence. Structures of the leaf blade, th...e upper and lower epidermis, the petiole have been also observed. The leaf blade of cotyledons already contains a typical palisade as well as spongy parenchyma tissues, i.e. being bifacial showing a structure similar to that of the true leaf. However, the petiole displays differences from the true leaf. There are a narrow (4-5 layer) primary cortex and a tiny central cylinder. Primary leaves bear already hairs on the adaxial surface and the transporting tissue-bundles are recognised in cross sections having a "V" shape. The first true leaf composed by three leaflets is of a simple structure showing characters reminding of cotyledons and primary leaves. Leaves of intermediate size continue to grow, whereas their inner anatomy changes dramatically. In the central region of the leaflets, near to the main vein, a second palisade parenchyma appears, further on, transporting tissue bundles are branching in the petiole. Collenchyma tissues enhance the stiffness and elasticity of the petiole. Older true leaves develop thick collenchyma tissues around the transporting bundles being represented by increasing numbers. The doubled palisade parenchyma layers of the leaf blades are generally observed. The cataphylls of the runners have a more simple structure, their mesophyll is homogenous, no palisade parenchyma appears. It is evident that leaves grown at successive developmental stages are different not only in their morphological but also anatomical structure. There is a gradual change according to the developmental stage of the leaves.
Efficacy of a popular synthetic detergent, Surf excel in some important vegetable crops viz. Okra or lady finger (Abelmoschus esculentus L.), chilli or red pepper (Capsicum annuum L.) and tomato (Lycopersicon esculentum Mill) was evaluated for inducing male sterility and hybrid seed production. Foliar sprays with aqueous solutions of Surf excel (1....0 and 1.5% w/v) in these crops induced complete pollen sterility. The treated plants showed a delay in flowering, a reduction in the number of flowers and fruits/plant, number of seeds/fruit resulting in a reduction in yield/plant. However, the male sterility thus induced was successfully exploited for hybrid seed production.
The present paper evaluates the result of irrigation experiments carried out on snap beans sown in spring and summer and grown with and without irrigation. The experiments were run over the course of 12 years. In the average of 12 years, the yield was 2.8t ha-I for spring sown and 1.9 t ha-I in summer-sown plants without irrigation. The lowest lev...el of profitable production, the 5.5t ha-I was reached twice in the case of spring sowing and only once in the case of summer sowing. Profitable yield production can be ensured only with regular irrigation and thus the yield may be increased by 4-5 times. In four of the twelve years we determined the canopy surface temperature of snap bean stands with and without irrigation. A Raynger II infrared remote thermometer determined the canopy surface temperature every day at 13.00 hours. The canopy temperature can well characterize the water supply of plant stands. This parameter may be used for describing the degree of drought and the water turnover of plant stands with different water supply. The positive values of foliage-air temperature differences (SDD) numerically express the degree of drought and the water supply of the crops. The results indicated that a 1 °C higher SDD value may cause 90-130 kg/ha yield loss.
Allyl isothiocyanate baited traps to monitor cabbage flea beetles (Phyllotretra spp., Coleoptera: Chrysomelidae)
A new trapping concept has been proposed based on a volatile compound, allyl isothyocianate, known to be attractant to some of these insects for a long time. (l) The first question was whether this compound is effectively attractive to all flea beetle species attacking cabbage under our conditions Field experiments were made at different localiti...es with non-sticky baited traps early and late spring. Eleven Phyllotreta species attacking cabbages were captured at baited traps most of them were first observed at this bait. So the bait has proved to be sufficient for use for trapping purposes effectively. Based on these findings a second question arose whether the captured samples reflected the specific composition of natural flea beetle populations at trapping localities To reply the question field samples were taken at four different kinds of cabbage crops and at a fallow ground in the close vicinity by a manual sampler device suitable to detect the local composition of flea beetles and trapping was made parallel with baited and unbaited traps from early spring to early autumn. No significant differences were found between the specific structures of Phyllotreta assemblages sampled with the different methods applied. This means baited traps reflected the specific composition of local Phyllotreta populations fairly well. Thirdly, the most effective trap design was searched for. Some sticky and non-sticky trap designs which had been developed to capture other insects were compared. The tested sticky and funnel trap designs baited with allyl isothiocyanate captured large numbers of flea beetles attacking cabbages. Results showed that non-sticky funnel traps were more effective than sticky delta traps. Accordingly, non-sticky funnel trap designs can advantageously be used and could possibly be recommended in plant protection practice to monitor flea beetles attacking cabbages as their catching capacity is considerably greater than that of the delta type and additionally captured beetles are much cleaner, more intact and consequently their identification is much easier.
Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA
Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-li...fe has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.
The aims of our work were to answer the following questions: 1. Diquat dibromide at different concentrations is a contact or systemic herbicide 2. if translocation occurs what is the extent and direction of it. 3. by what concentration it is translocated in hydroponics. It has been stated that diquat dibromide at different dilutions (40-5%) had sy...stemic effect in Robinia pseudo-acacia in the fields. Its translocation has been occurred apically first, and later basipetally. The extent of translocation depended on the concentration. In hydroponics diquat dibromide has crytallized on the leaf surface of Galinsoga parvif lora and all concentrations (40-0.078%) proved lethal. Recognition and application of systemic characteristics of diquat dibromide is reported here for the first time. Improvement of application method is in the focus of our future work.
There has not been any research done on the musts from organic grapes. As a result of the special technologies in organic farming we can expect bio-musts to have a different quantitative and qualitative composition of nitrogen compounds from that of traditional musts. In our present essay we deal with the tests of nitrogenous compounds (assimilabl...e nitrogen content, aminoacid, biogenic amine content) in musts from Hungary's bio-wine cellars. Our results show that the change in procedures has resulted in neither quantitative nor qualitative changes in the nitrogenous compounds of bio-musts.
There has been a growing tendency in organic farming in recent years, in which organic grape growing and the production of biowine are also significant. The literature on bio-wine is rather limited, that is why we consider it really important to contribute to the data. As a result of the special conditions in organic farming we can expect bio-wi...nes to have a different composition of nitrogen compounds from that of traditional wines. Biogenic amines could thus specially characterize bio-wines. In our research we compared the biogenic amine content of wines produced using organic methods and of those where traditional procedures were used. Our measurements have proved that it is not possible to clearly distinguish bio-wines from wines produced with traditional methods on the basis of amine content.