Vol 5 No 3-4 (1999)
Cikkek

The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon

Published September 13, 1999
Z. Qiu
University of Department of Horticulture and Food Industry Genetics and Plant Breeding, H-1118 Budapest, Ménesi út 44, Hungary
Cs. Bársony
University of Department of Horticulture and Food Industry Genetics and Plant Breeding, H-1118 Budapest, Ménesi út 44, Hungary
Gy. Bisztray
University of Department of Horticulture and Food Industry Genetics and Plant Breeding, H-1118 Budapest, Ménesi út 44, Hungary
I. Velich
University of Department of Horticulture and Food Industry Genetics and Plant Breeding, H-1118 Budapest, Ménesi út 44, Hungary
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APA

Qiu, Z., Bársony, C., Bisztray, G., & Velich, I. (1999). The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon. International Journal of Horticultural Science, 5(3-4), 46-49. https://doi.org/10.31421/IJHS/5/3-4/42

Abstract

Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.

 

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