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Genetic transformation of bean callus via Agrobacterium- mediated DNA transfer
49-53.Views:130Callus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus co-cultivated with A. tumefaciens can be transformed to get heibicide Finale (glufosinate-ammonium) resistant GUS positive tissues. Southern blot analysis of transformed calli showed integration of gusA marker gene carried by a binary vector. Transformed calli were selected on herbicide containing media. Data of molecular analysis (Southern blotting) confirmed the insertion of gusA gene in the genome of herbicide resistant calli with bar gene. There are three evidences that calli are stable transformants: (1) herbicide resistance, (2) GUS activity which is indicative since the coding region containing an intron, (3) the results of Southern hybridization technique.
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Comparison of weed management methods in organic carrot
55-58.Views:15114 combinations of mechanical and also physical (thermal) weed management techniques are compared for organic growing of carrot. Crop of our weed management research is carrot because of its difficulties in weed management (long growing period, poor weed tolerance) and because carrot needs to be important product of organic farming. Herbicide treatment is used as control — cultivator, brush hoe, hand hoeing and hand weeding are mechanical control tools and flame weeder is used for thermal control. Measured parameters are the weed cover, cover of the crop and dry mass of them. Result of the two appraisable years shows contradictory results, which contradiction can be justifiable with different weather conditions of these two vegetation periods. In 2000 brush hoe was significantly the best in interrows but in the year 2001 cultivator combined with hand weeding in rows seemed to show the best result. We can see in this example that agriculture and weed management depends very much on the weather of the year, so that is why it is so difficult to develop a method, which can be generally used for organic weed control of carrot.
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The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon
46-49.Views:184Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.
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Translocation of diquat dibromide
105-107.Views:146The aims of our work were to answer the following questions: 1. Diquat dibromide at different concentrations is a contact or systemic herbicide? 2. if translocation occurs what is the extent and direction of it. 3. by what concentration it is translocated in hydroponics. It has been stated that diquat dibromide at different dilutions (40-5%) had systemic effect in Robinia pseudo-acacia in the fields. Its translocation has been occurred apically first, and later basipetally. The extent of translocation depended on the concentration. In hydroponics diquat dibromide has crytallized on the leaf surface of Galinsoga parvif lora and all concentrations (40-0.078%) proved lethal. Recognition and application of systemic characteristics of diquat dibromide is reported here for the first time. Improvement of application method is in the focus of our future work.