Vol 6 No 4 (2000)
Cikkek

Callus induction on standard type Cymbidium cultivars

Published August 23, 2000
L. Jánvári
Szent István University, Faculty of Horticultural Science, Department of Genetics and Horticultural Plant Breeding, H-1118 Budapest, Ménesi út 44. Hungary
Gy. Bisztray
Szent István University, Faculty of Horticultural Science, Department of Genetics and Horticultural Plant Breeding, H-1118 Budapest, Ménesi út 44. Hungary
T. Füzesi
Plantlab; H-9722 Perenye, Béke u 140.
I. Velich
Szent István University, Faculty of Horticultural Science, Department of Genetics and Horticultural Plant Breeding, H-1118 Budapest, Ménesi út 44. Hungary
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How to Cite

APA

Jánvári, L., Bisztray, G., Füzesi, T., & Velich, I. (2000). Callus induction on standard type Cymbidium cultivars. International Journal of Horticultural Science, 6(4), 108-110. https://doi.org/10.31421/IJHS/6/4/236

Abstract

Tissue cultured Cymbidium PLBs (protocormlike body) were used as starting material to induce embryogenic callus which could serve as objects of genetic transformation. We obtained callus using two methods. The first method was culturing the PLB segments for one month in liquid MS medium in the presence of 0.5 mg/1 benzyladenine and 0.05 mg/1 naphtylacetic acid followed by cultivation on the same composition solid medium with 0.5 g/l activated charcoal for an additional month. Callus formation was observed on 30% of the explants. The second way was to propagate the PLB segments on solid MS medium supplemented with 1 mg/1 thidiazuron. In these cultures we also observed callus formation on 20% of the explants.

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