Vol 5 No 1-2 (1999)
Cikkek

Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.)

Published May 24, 1999
E. Eissa Ahmed
University of Horticulture Dept. of Genetics and Plant Breeding, H-1775 Budapest, P. 0. Box 95, Hungary
Gy. Bisztray
University of Horticulture Dept. of Genetics and Plant Breeding, H-1775 Budapest, P. 0. Box 95, Hungary
I. Velich
University of Horticulture Dept. of Genetics and Plant Breeding, H-1775 Budapest, P. 0. Box 95, Hungary
View:
pdf
How to Cite

APA

Eissa Ahmed, E., Bisztray, G., & Velich, I. (1999). Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.). International Journal of Horticultural Science, 5(1-2), 57-60. https://doi.org/10.31421/IJHS/5/1-2/24

Abstract

Dry seeds from two cultivars of common bean (Phaseolus vulgaris L.) were germinated on sterile cotton and sterile deionized distilled water. Cotyledonary node tissue of seedlings were cultured on Murashige and Skoog(MS)-based media supplemented with different combination of N6-benzyl-aminopurine (BAP) and indole-3-acetic acid (IAA), and benzyladenine (BA) and a-naphthaleneacetic acid (NAA). The results revealed that the regeneration percent and the average number of buds and shoots per explant were influenced by the type of explants and exogeneously added hormones. Multiple shoot induction on dry bean cotyledonary node that contain 4-5 mm from cotyledons and hypocotyl on a medium containing full concentration of MS inorganic salts supplemented with 0.5mg/1 BA and 0.1mg/1 NAA was feasible and the method can be applied in transformation experiments.

 

Downloads

Download data is not yet available.