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• Interactive effect of sulphur and lead on the growth of muskmelon (Cucumis melo L.) seedling

  72-76.

  A. El. B. Ragab

  Views:

  124

  The effect of sulphur and lead on growth of muskmelon (Cucmis melo L.) sweet ananas was measured under plastic tunnel. Lead (Pb) was applied at the rate of 0, 75, 300, 600 and 1200 pg/g of soil in factorial combination with treatments of 0, 48, 80, 112 and 224 mg S/kg soils. Muskmelon growth was reduced by increasing Pb concentration and/or by removing S from the growth medium. Lead concentration in plant tissues increased linearly with increasing Pb concentration in the growth medium. Increasing S concentration in solution reduced the effects of the 75-1200 pg Pb /g soil treatments. Increasing S concentration in soil increased shoot and root growth at high levels of Pb. Increased levels of S in the growth medium decreased Pb concentration in shoot and root tissues and increased growth of muskmelon. The reduction in Pb toxicity at high S supply may be due to reduced root absorption of Pb.

   

• The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon

  46-49.

  Z. Qiu

  Cs. Bársony

  Gy. Bisztray

  I. Velich

  Views:

  184

  Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.

   

• Shoot induction and plant regeneration from cotyledon segments of the muskmelon variety "hógolyó"

  61-64.

  Cs. Bársony

  Gy. Bisztray

  E. Bába

  E. Velich

  Views:

  151

  Cotyledonary segments of the casaba type muskmelon variety "Hógolyó" were used to induce organogenesis. Fifty different hormone combinations were applied to enhance the induction of shoot formation on the edge of the segments. The phases of organogenesis were followed with light- and scanning electron microscope. Shoot induction was achieved with high frequency. The shoots were transferred to hormone free media for root induction. The rooted plantlets were planted out to soil.

  NAA was feasible and the method can be applied in transformation experiments.