Search
Search Results
-
Practical experiences of a designing and operating a pilot aquaponic system
27-32Views:237Aquaponics is the combination of fish farming (aquaculture) and the soilless cultivation of plants (hydroponics). The aquaponics system is an artificial, recirculating ecosystem, in which bacterial processes convert the waste materials in the water used for fish rearing into plant nutrients, and therefore with the generated heat it is suitable for culturing economically valuable plants, and thus it mitigates the nutrient laden and quantity of the intensive fish producing systems’ effluent water.
The primary goal of our 12 separate unit’s aquaponics system was to gain experience. We would like to find the right plant species, which are fit for that medium, and their crop can be sold. Besides the plants, our attention focused on the fish. Two fish species were included in the experiments, the common carp and barramundi. It was difficult to create them a perfect living space, besides a constantly changing conditions temperature. Apart the above mentioned we had a problem with the number of individuals per tank, the deformity of the fish body and the too high volume of pH (we registered continuous values above 8.4). We get by carps 4.7 grams of weight gain during 15 weeks, because of the bad conditions.
The main problems at the plants are caused by aphesis and protection against sunburn. Even so we have got the multiples of field yields for each plant species. At salad has grown twice of field yields, tomatoes one and half, kohlrabi than 3.5 times more. The causes of multiple yields are the continuous balanced water and the nutrient uptake of plants. Each plant species fit for cultivating in aquaponics and their crops are delicious, chemical -free, safe and marketable. The plants should be more concentrated. After the experiment, it has been determinated that the carp is suitable for aquaponics, but greater weight gain could be achieved with optimal selection of size of rearing units.
-
The effects of agrotechnological factors on winter wheat yield in humid cropyear
162-167Views:77The effects of crop rotation, nutrien supply and crop protection technologies, as well as the appearance of the main ear- and leafdiseases
(powdery mildew, helminthosporium leaf spot, leaf rust, fusarium) were studied on the crop yields of winter wheat variety MV
Pálma during the 2009/2010 crop year. The experiments were conducted in triculture (pea – wheat – corn) and biculture (wheat – corn), at
five nutrition levels, with the use of three crop protection technologies (extensive, conventional and intensive) at the Látókép Research Site of
the University of Debrecen, Centre of Agricultural Sciences. Our results proved that the appearance of leaf- and ear-diseases were
significant in the wheat cultures during the 2009/2010 crop year, because of the rainy, warmer than usual weather, the lodging, and the huge
vegetative mass developed. The most severe infections by the four examined diseases after pea and corn pre-crops were observed at
extensive crop protection levels, when fertilizers were used at the highest dose.
Following corn pre-crop, in the case of all the three crop protection technologies the maximum rate of wheat yield results were achieved
at N150+PK level. The highest yield was reached at intensive crop protection level (6079 kg ha-1). In triculture, in case of all the three crop
protection technologies the maximum yields were achieved at N50+PK level; in extensive technology 5041 kg·ha-1 yield, in conventional
technology 6190 kg ha-1 yield was realised, while in the intensive technological model the yield was 7228 kg ha-1.
The relationship between yield and fertilizer amounts, the rate of pathogen contaminations, crop protection technologies and pre-crops
was defined with correlation analysis in case of different crop rotations during the 2009/2010 crop year. Based on the results of the
experiment, we found that in stands after corn pre-crop strong positive correlation was established between the crop protection level and the
crop yield (0.543), the nutrient levels and the emergence of the four examined pathogens, and between the nutrient levels and the yield
(0.639). Extremly strong positive correlation was observed between crop protection and yield (0.843) in triculture. Strong positive
correlation was detected between the nutrient levels and the presence of the four examined pathogens, as well as between nutrient and
lodging (0.688). Strong negative correlation was between the crop protection level and the four examined diseases both in biculture and
triculture. -
Comparison of the alveographic qualification and the baking quality of winter wheat
294-302Views:129On the basis of the operative French regulations, we qualified the alveographic qualification and the valorigraphic qualification respectively for each of the 10-14 winter wheat varieties grown each year between 2002-2005, and used in a field experiment at the Karcag Research Institute of the University of Debrecen. The field experiment at the Látókép Research Institute of the University of Debrecen allowed us to examine the alveographic and valorigraphic values of the samples of GK Öthalom and Fatima 2 winter wheat varieties taken from an 8 year period (1997-1999 and 2001-2005).
Throughout the experiments on samples from the Karcag Research Institute and Látókép Research Institute of the University of Debrecen, we tried to find whether the alveographic W value or the P/L value provides the safer method for the qualification of wheat samples, instead of the qualification commonly used in Hungary. We found that in terms of the samples from the Karcag Research Institute, the baking quality and the W value in each of the examined years, while in terms of the Látókép Research Institute, most of the years showed a close positive correlation, contrary to the close negative relationship between the baking quality and the P/L value. The correlation index varied depending on the year, the variety, and fertilizer doses, which require further examinations. -
Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:143Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
-
Woolly cupgrass (Eriochloa villosa /Thunb./ Kunth), a recently occured invasive weed in Trans-Tisza Region and a trial for control in maize
53-57Views:241To the effective control of invasive weeds are essential to prevent establish, if has already happened obstacle to massive accumulation, and promoting the efficient and rapid eradication, if it is possible. The Woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) belongs to weeds which “hard to control” especially in corn. One of the difficulties of effective control is the prolonged emergence causing avoidance of several individuals the contact with pre-emergent herbicides. Another problem arises due to the intensive use of post-emergence herbicide products with short duration of action. To optimalize of timing of treatment is essential for successful control of later emerging weeds. The recently established Woolly cupgrass in Hungary shows resistance or reduced susceptibility to substantial portion of herbicides used in corn. The data collected from small-plot trials demonstrates that application of sulfonylurea or selective monoctyledonous herbicides can be effective against the Woolly cupgrass.
-
Evaluation of decreasing moisture content of different maize genotypes
147-151Views:225An experiment was conducted to evaluate the decrease in grain moisture content in three maize hybrids in Debrecen in 2017. Armagnac, Sushi and Loupiac were the examined hybrids in this study. The culture medium and temperature conditions were applied uniformly for all three hybrids. According to the results obtained from the ratio of moisture content of seeds per day, the Armagnac variety in the intensive drying down phase loses more time and moisture content, so it can be concluded that the produced dry matter is more than in the case of other varieties. Armagnac requires more time to achieve yield, while the Sushi and Loupiac hybrids produce less harvest. Regarding the slope of the regression line, the rate of loss of moisture in the grain has been negatively correlated with the amount of “b” in the three examined hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97% in the Loupiac, 95% and in the Sushi 90% of the total dynamic value of moisture motion.
-
Effects of fermented and supplemented chicken manure on the nutrient management aspects of an apple orchard
117-123Views:75AIt is a huge challenge for farmers worldwide to successfully increase the organic matter content of their soils and improve their water balance at the same time. Therefore, the main aim of the study is to develop and test organic-based nutrient composite materials that can be successfully used by farmers to increase soil organic matter content, improve water management parameters and implement water-efficient technologies. The study was performed in the orchard of the Institute of Horticultural Science of the University of Debrecen in Hungary (Debrecen-Pallag). The experiment was set up in a ten-year-old apple (Malus domestica ‘Pinova’) orchard. In the trial, fermented poultry manure and superabsorbent polymers (SAP) were used at different doses to study their effects on soil properties and fruit quality. Applied composite materials increased the nitrate and organic nitrogen content of the soil. Treatments did not affect the sugar content of the fruits but significantly and positively affected the individual fruit weight and the titratable acidity of the fruits.
-
The importance of selenium in the living world and in the scientific research
278-286Views:137Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Susceptibility of stem infected sweet corn hybrids to common smut disease
55-57Views:370The common smut of maize (corn smut, Ustilago maydis /DC/ Corda) can cause large economic losses in susceptible sweet corn hybrids as well. The protection against this pathogen is fundamentally based on prevention. Many methods to control corn smut have been recommended or evaluated, including crop rotation, sanitation, seed treatments, modification of fertility, and biological control. In spite of these frequently mentioned control strategies, the host resistance seems to be the only effective method to manage common smut in those areas where Ustilago maydis is prevalent.
-
Effect of Rootstocks on Blooming Capacity and Productivity of AppleCultivars
14-20Views:98The experiment with three different rootstock cultivars was set up in a commercial apple orchard at Nagykutas, which is situated in the western part of Hungary. The aim of our two-year-study was to determine the effect three different growing rootstock cultivars (M9, MM106 and seedling) on the flowering and productivity of 33 apple cultivars. Our observations included the following measurements: the date of the beginning and the end of flowering, flower density, fruit density, fruit numbers per tree and tree productivity. According to our results, it was found that the different growing rootstocks have a great determining effect on the above measurements. Our results showed that the flowering period was similar for all cultivars on the three different rootstocks. However, the flowering and the fruit setting decreased in the order M9, MM106 and seedling rootstocks. In contrast, the fruit number per tree followed, in decreasing order, MM106, seedling and M9 rootstocks.
-
Nest-site preference of Hooded Crow (Corvus cornix L.) in Debrecen, Hungary
13-17Views:117In the past decade, the population of the Hooded Crow has shown significant growth in Debrecen, Hungary. The aim of this study was to become acquainted with the nest-site selection behavior of Hooded Crows in urban spaces. While our research revealed that the Hooded Crow is not particular when it comes to selecting among tree species for nesting, we did notice differences regarding nesting height. Hooded Crows living in the city build their nests higher up than those living outside Debrecen’s city limits. We also discovered a slight, insignificant difference between nesting heights and tree species, which is probably due to the different characteristics of the given tree species. As for nesting heights, we found that in typical urban habitats there were no relevant differences. However, when we compared these habitats in pairs, it came to light that nesting heights -when comparing solitary trees - wood segments and tree rows - wooded segments- did show significant differences, which can be explained by the various conditions provided by the habitats mentioned.
In summary, the following results emerged from our research:
1. The Hooded Crow prefers approximately the same nesting heights in all kinds of habitats, urban environment and tree species.
2. The nesting height does not significantly depend on the habitat itself or on the tree species. -
Examination of the American grapevine leafhopper (Scaphoideus titanus Ball) in Debrecen and Micske (Misca, West Romania)
77-81Views:133Grapevine flavescence dorée (FD) was detected first in Hungary in 2013 in Zala County (South-West-Hungary). The disease is a serious danger for grapevine growing and grapevine propagating production. In 2014, the pathogen has been found in several new places in Hungary, viz. in Vas and Fejér Counties, and it was also detected in the former location in Zala County. The american grapevine leafhopper (Scaphoideus titanus) is the main vector of the disease. This pest was detected first in Hungary in 2006 and then it has spread all over the country. Since we have not detailed distribution data of this pest in surroundings of Debrecen, therefore we made observations in this region in 2014. The presence of the pest was confirmed by yellow sticky cards in two locations in Debrecen and another site in West Romania near to Hungarian border. We found that S. titanus is present in each sampled sites that cause serious potential danger for the appearance and spread of Grapevine flavescence dorée (FD) in this region.
-
Preliminary estimation of the efficacy of Fusarium sporotrichioides Sherb. as biological control agent against common milkweed (Asclepias syriaca L.)
201-204Views:248A study of fungi responsible for severe leaf spots of common milkweed (Asclepias syriaca L.) in the Hajdúság region (East Hungary), Fusarium sporotrichioides and Alternaria alternata were isolated from infested leaf tissues. F. sporotrichioides was the most virulent fungus in pathogenicity tests conducted on healthy leaves of common milkweed plants. Inoculation of common milkweed (A. syriaca) in different growth stages with F. sporotrichioides yielded similar symptoms as the original ones. Spray mixtures containing 1.0×106 conidia/ml gave effective control when common milkweed plants were sprayed until runoff occurred. Laboratory (wet chamber) and field experiments showed that asexual spores of the fungal pathogen, F. sporotrichioides, exhibited bioherbicidal activity against common milkweed (A. syriaca).
More efficient control efficacy was observable on elder plants (at flowering stage) than younger ones. These results initiate that this fungus may be a biocontrol agent for controlling this invasive weed but should clarify its hosts because it could infect cultivated plants as well.
-
Ragweed components in honey
65-68Views:155The aim of this research was to prove that the syrup containing ragweed used for feeding the bees was transferred to the honey. To reach this goal we developed a method to analyse the volatile components of the samples. We applied two sample preparation methods. The first one was SPME (Solid Phase Micro Extraction) preparation from the headspace of all of the samples. In the second case only the raw leaves of the ragweed were extracted with n-hexane and dichloromethane to complete the SPME results. The measurements were performed on GC-MS equipment. Chromatograms and data evolution showed that the components of the ragweed were also present in honey so they were transferred by bees.
-
Chromatometric comparison of Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) feathers
19-22Views:176Chromatometric examination of the plumage of birds is a poorly researched topic. We have approached this issue in primarily aspect of differences in plumage of species. Moulted feathers sample collection method has been increasingly used. Reliable identification of feathers becomes an increasingly important issue, hence need for an exact measurement-based methodology. Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) primary, secondary and tail feathers were studied. Chromatometric parameters of feathers were measured in CIELAB color system and then statistical analysis (Independent samples t-test, Descriptive Statistics, Discriminant Analysis) was performed to compare the two species. Instrumental measurements has been confirmed the high similarity between colors of the two species, however species specific differences were also found. Lightless (L*) value were significantly characteristic of particular species, while the red/green (a*) and yellow/blue (b*) value had lower Predictive Power. We identified feathers and the variables which useable to separate the two species and determined the associated Confidence Intervals of these values. Our results may draw attention to a new potential direction for exact identification of the moulted feathers during sample collection.
-
Application of AFLP-Method in Plant Sample Identification
207-213Views:62One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.
-
Effect of the storage temperature onto the quality of the semen of rams of different breeds
75-79Views:114Semen of 10 Tsigai,3 Prolific Merino and 7 Barbados Blackbelly rams was taken in January, out of the season. Split samples of the diluted semen samples were kept at 23, v.s. 8oC and their motilitywas evaluated daily for 3 days by subjective microscopic investigation. The ratio of motile spermatozoa was strongly decreased during the first day, later the motility rate was sinking more slowly. It could be stated by multivariance analysis that the motility value of diluted semen of Prolific Merino rams was higher and showed a slower reduction as compared to the other two breeds. The decrease of the motility rates was slower at 8oC than at 23oC.
-
Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
35-40Views:148Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food, seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.
-
Characterization of two rust fungi related to biological control concept in Hungary
195-199Views:274Weeds cause serious problems in agriculture on a global scale. These plants reduce yield and the quality of crops by competing for water, nutrients and sunlight. The improper or excessive usage of herbicides have led to development of resistance in some weed species while contaminating the environment; therefore, biological control has an increasing role as an alternative method for controlling special weed species.
The aim of this study is to make a brief review of biological control of weeds by pathogens and to characterize two rust fungi (Puccinia lagenophorae and Puccinia xanthii) which are broadly examined recently in a biological control concept and have been found on their hosts, such as common groundsel (Senecio vulgaris L.) and common cocklebur (Xanthium strumarium L.), two common and difficult to manage weeds both in horticultural and agricultural lands also in Hungary.
-
The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:173Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
-
Examination of feral pigeon populations at animal breeding farms
52-56Views:81In the course of our examination, we assessed biometric data that determine gender, we evaluated sexual activity, measured the nourishment and necropsies of two feral pigeon populations feeding and nesting at animal breeding farms. The changes in the body weight of the different genders were significant on both farms (p<0.05).
The body weight and the crop volume of the birds were not significantly different at the examined farms.
On both farms, the elemental food source of the birds was the fodder and corn kernels given to cattle, and was supplemented by that of the pigeons living at the Debrecen farm with food from surrounding farms and with other seeds (pea, millet, rye).
By the examination of the genitals, the birds collected from the Nyírbátor farm showed sexual activity from the beginning of spring, and in the summertime decreasing activity is characteristic, while the pigeons from the Debrecen farm are only active from the summer months. In the course of the examinations in the Veterinary Institute of Debrecen, the birds proved to be free of parasites and the results of the Newcastle disease analysis were negative as well. The bacteriologic analyses showed a different proportion of E.coli, Pasteurella multicoda, haemolysing Staphylococcus bacteria at the two cattle farms. During the examinations for the statement of Salmonellas, this causative agent was stated only in case of birds that spend nights and nested in the town, which allude to human origin. We demonstrated Eimeria oocysts during the parasitological examination of the fecal samples in 5 cases at the Debrecen, and in 1 case at the Nyírbátor farm. -
Examination of the interval between litters (IBL) of different genotype HLW sows using survival analysis
13-17Views:157In this study our aim was to find out if there is a difference between the genotypes determined for the previously identified mutations of seven genes of the Hungarian Large White in terms of the time spent in production. We identified the previously determined alleles of the seven genes (BF, EGF, ESR, FSHβ, H2AFZ, LEP, PRLR) related to proliferation that were and performed the survival analysis between breeds indicating the risk of culling and the time spent in production on the given farm. Based on the results of survival analysis by Log-rank test, Breslow (Generalized Wilcoxon) and Tarone-Ware test we concluded that they indicated a significant difference in case of the genes BF (Breslow and Tarone-Ware tests) the EGF (Log-rank and Tarone-Ware tests) and ESR (Log rank test) based on which the curves of the survival of the certain genes varied form one another significantly.
-
Distribution of the American grapevine leafhopper (Scaphoideus titanus ball 1932) in west Romania
127-130Views:154The vector of Grapevine Flavescens Dorée phytoplasma, the American grapevine leafhopper (Scaphoideus titanus) has been in Europe since 1924. In Romania, the first populations were detected in 2009 in the central, eastern and southern part of the country. Later, the leafhopper was found also in West Romania in 2014. In 2015 and 2017, altogether, 14 sampling sites were studied in two vine regions of this area. The Scaphoideus titanus could be detected in 10 of them with relatively small abundances. During the studies, the yellow sticky traps proved more effective methods than sweep netting. To prevent vineyards from disease, we should do everything against the vector from prevention to chemical protection.
-
Introduction of DNA-based Methods to Agriculture Through Molecular Taxonomic Examination of Poa Species
139-142Views:83biological methods, one among them is AFLP that is well applicable for taxonomic research. Bluegrass species, that are important components of meadow associations, thus their thorough knowledge is necessary in maintaining biodiversity, were examined with bringing this method to perfection.
Taxonomic relationship of the members of Poa pratensis aggregation is a controversial issue. Present study aimes to identify the members of this group, with a developed AFLP method through molecular taxonomic examination of Poa species in meadows nearby Debrecen, revealing their genetical distances. Species of the aggregation show a great genetic variability, but their genetic proximity approves the use of the term aggregation. Results established wider geographical investigation of three species of the aggregation. The distinctness of the species based on their morphological features was confirmed according to their genetical basis as well. The method overall turned out to be appropriate for the taxonomic research of bluegrass species like Poa pratensis and its aggregation. -
Identification and biological examination of some inland Monilinia species
27-29Views:113The aim of this study was to identify and biologically analyse some Monilinia species from Hungary. 146 M. fructigena and 28 M. laxa species out 174 infectious fruit from all over the country were used for the study. For further study 10 isolates were used and apple fruit was inoculated with them according to Koch postulate. 1–2 mm ochre exogen stromas were observbed on infectious plant parts and growing signs on culture of all isolates were identical to M. fructigena. To affirm classical identification, isolates with molecular biological method were also prepared using PCR reaction. Control isolates of M. laxa, M. fructigena and M. fructicola were used. The size of PCR product showed that all isolates had a 415 bp band which was typical of M. fructigena. Results support the previous observation that M. fructigena and M. laxa species occure all over Hungary.