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Ragweed components in honey
65-68Views:155The aim of this research was to prove that the syrup containing ragweed used for feeding the bees was transferred to the honey. To reach this goal we developed a method to analyse the volatile components of the samples. We applied two sample preparation methods. The first one was SPME (Solid Phase Micro Extraction) preparation from the headspace of all of the samples. In the second case only the raw leaves of the ragweed were extracted with n-hexane and dichloromethane to complete the SPME results. The measurements were performed on GC-MS equipment. Chromatograms and data evolution showed that the components of the ragweed were also present in honey so they were transferred by bees.
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Changes of legal forms at professional football ventures
141-146Views:142Since the ’90s professional football has been going through an unprecedented economic growth. The football ventures are becoming more and more middle-sized companies. In football there are organizational changes, which are reflected in changes of legal forms of professional football clubs, and in using of modern controlling, planning, risk and financial management. In this paper we primarily wish to show the specificities of legal forms in German professional football, because we believe the German model is the best example for the future development of football around the world, and we believe this would be most advantageous towards our future development at home.
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The marigold (Calendula officinalis L.) drug essential oil agents change under different fertilization settings in small plot trial
57-60Views:158During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization setting in small-plot trial. We measured SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) we examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.
Based on the results, it was concluded, the essential oil agents' percentage breakdowns significantly depending on the cropping technologies. Besides that it is possible, based on Pearson's correlation test the marigold essential oil agents relationship can also be a major factor.
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The examination of the marigold’s (Calendula officinalis L.) nutrient requirement in small-plot trial
61-66Views:150During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization treatments in small-plot trial. We measured the harvested marigold's drug's raw and dry weight on a weekly basis from July 6th until August 17th. We were using SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) to examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.
It was concluded, based on the results, the N30P40K60 fertilization setting is ideal in terms of the quantity of the marigold drug. Both the raw and the dry weights' measurements of the case, this fertilization setting have the most important effect on the herb's yield. The analysis of variance didn't show significant differences between the plots with different fertilization settings. We discovered relationship between the drying loss and the increasing quantities of nutrients. We think it may be possible the Alpha-thujon's and Alpha-cadinol's production and the drying loss's data are connected, which appear to confirm the N15P20K30 treatment's data.
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HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:240Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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Determination of conversion rate values to characterise the ethanol production of the Kluyveromyces marxianus CBS712 fungus branch
23-27Views:70The ethanol production of the Kluyveromyces marxianus CBS712 strain was investigated among different kind of condition. We defined the conversion rate in order to know the efficiency of the ethanol production. To determine this value, it is crucial to characterize the residual glucose concentracio. We chose two method to determine the amount of residual glucose. The first method is the thin layer chromatography (TLC) and the second method is the method of the glucose-oxidase enzyme. We found that the TLC method is reliable than the other method. The conversion rates were determined from these values and the ethanol values. The maximal ethanol production of the characterized Kluyveromyces marxianus CBS712 strain (5.6% (v/v) ethanol at 45 °C, 55.3% conversion rate) is comparable to those strains which are applied in industrial ethanol production nowadays.
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Effect of compost/water ratio on some main parameter of compost solutions
117-121Views:185Composting is an efficient technology for the utilisation of by-products and waste. It is also suitable for treating raw materials to convert fertilisers that are not recommended for application without pre-processing. Such is the case with poultry manure, which is very important to pre-treat due to its hazardous properties. An increasingly common form of compost is compost tea, which is made by soaking compost in water. In our experiment, we made compost tea from a composted and granulated poultry manure product. Three mixing ratios were used (1/5, 1/10, 1/10) and compost teas were set for three different extraction times (24–48–72 hours). After elimination of the experiment, the pH, EC, and nitrate and ammonium contents of the samples were measured. The aim of this study was to determine whether there is a difference between each mixing ratio and different extraction times. Based on our results, it can be said that the fluctuation of pH values during the experiment was low. Regardless of the mixing ratio and extraction time, the samples were in the slightly acidic range. The electrical conductivity, nitrate and ammonium content follow a similar trend, the values decrease significantly with the increase of the mixing ratio. Similar tendency was observed at the incubation time. The concentrations of both EC and nitrogen forms increase with increasing incubation time, in most cases significantly.
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The importance of selenium in the living world and in the scientific research
278-286Views:137Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Examination of the effect of pelleted poultry manure products on a sunflower test plant in a laboratory model experiment
83-88Views:166Sunflower (Helianthus annuus L.) is one of the most important oil plants in Hungary, with a sown area of more than 627.000 ha in 2019. Sunflower cultivation is primarily important for use in the oil industry, but its role in feed and beekeeping cannot be neglected either. Sunflower adapts well to different production areas in terms of soil demand, yet, as important fact, it must be grown on soils with poorer conditions with intensive nutrient replenishment. From the agri-environmental point of view, in addition to the use of pelleted poultry manure products, which are also new to the consumer market, is of paramount importance. Their advantages include a wide range of nutrients as well as a soil structure improving effect.
In our research, we investigated the starter effect of pelleted poultry manure products applied at different doses (200 kg ha-1 and 400 kg ha-1) on sandy soil with humus using a sunflower plant. The aim of our research to investigate the effect of using pelleted fertilizer products as starter fertilizers on sunflower plant germination, and investigate the effect of poultry manure products on soils.
Based on our results, it can be stated that the pigment content (chlorophyll and carotenoid content) was positively influenced by the higher dose treatments (400 kg ha-1), thus increasing the photosynthetically active leaf area. Compared to the control, the carotenoid content in the test plants of the treatments increased, and the differences of this treatment proved to be significant (p<0.05). Our experiments supported the beneficial effect of pelleted poultry manure-based products in the soil-plant system.
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Characterisation of a thermotolerant yeast, Kluyveromyces marxianus CBS712
7-13Views:97Fermentation at high temperature with application of thermotolerant microorganisms is a technological advantage in bioethanol production. Among the yeasts, K. marxianus has outstanding thermotolarance. The industrial application of the IMB3 strain occurs usually at 45C. The final aim of our project is the genetic modification of the K. marxianus CBS712 strain in order to achieve ethanol production at higher temperature than the currently applied. This requires the characterization of the CBS712 strain, with special attention to the determination of the temperature limit of its growth and the amount of the ethanol produced. The temperature limit of growth was 48C in YPD medium. Elevation of the temperature above 45C led to an exponential drop of the cell viability. Ethanol production was tested in shaking flasks, in MYFM medium, under oxigene limited conditions, applying variable concentrations of glucose (12–20%) and different temperatures (45–47 ºC). Preliminary results have revealed that the elevation of glucose concentration increased the amount of ethanol produced. The amount of ethanol (appr. 5%)+ produced at the highest glucose concentration was not different at the tested temperatures (45, 46 and 47 ºC). The observation indicates the potential in raising the thermotolerance of the strain.