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  • Lessons of a stripe rust epidemic in a wheat fungicide trial in Debrecen
    35-46
    Views:
    135

    During the 2014 year a fungicide application trial was made as a part of technology development in Debrecen (East-Hungary). Both in this trial and across in Hungary a serious stripe rust epidemic developed and caused great yield losses. The first sympotoms were observed in April on one of trial plots and during some following weeks a serious infection grown up. On the base of 13 fungicide active ingredients and dosages by two spraying applications, their efficacy could be evaluated in stripe rust control. Picoxystrobin 250 g/L acive ingredient applied in 0.6, 0.8 and 1 L/ha dosages (Acanto), picoxystrobin 200 g/L + ciproconazole 80 g/L combined active ingredients in 0.5, 0.75, and 1 L/ha dosages (Acanto Plus), epoxiconazole 83 g/L, moreover protioconazole 125 g/L + tebuconazole 125 g/L combinations gave good efficacy, respectively. The yield of well protected plots were 5 to 8 t/ha, but the losses were 40-70% when fungicides with no sufficient efficacy in stripe rust control were applied. The great infection, which was observed on different grasses late in the Autumn, 2014 might forecast a further stripe rust epidemic for the 2015 year.

  • Irén Dobos horticulturalist-teacher plant protection engineer expert awarded by Antal Gulyás medallion for crop protection" in 2015 (laudation)
    8-10
    Views:
    167

    The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors established a joined Award Committee in 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by awarding the „Antal Gulyás medallion for crop protection” which are available for outstanding teachers, researchers, and practical crop protection specialists. In 2015 dr. Irén Dobos horticulturist- teacher and plant doctor has been decorated with the „Antal Gulyás medallion for crop protection” for her “excellence in integrated plant protection practical research activity and effective knowledge transfer”.

  • László Szabó herbologist awarded by „Antal Gulyás medallion for crop protection“ in 2014 (laudation)
    5-7
    Views:
    246

    The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors (Chamber) established a joined Award Committee in 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by awarding the „Antal Gulyás medallion for crop protection” which are available for outstanding teachers, researchers, and practical crop protection specialists.

    In 2014 László Szabó herbologist has been decorated with the „Antal Gulyás medallion for crop protection” for his “excellence in applied herbology and practical research activity on herbicide applications, moreover for knowledge transfer on weed management”.

  • A morphological survey of Ustilago trichophora, a smut fungi and evaluation as bioherbicidal agent for barnyard grass (Echinochloa crus-galli)
    91-93
    Views:
    143

    Ustilago trichophora (Link) Kunze is a widespread smut fungus in all over the world. This fungus is pathogenic on species of Echinochloa genus. The subject of present research was that smut fungi occurrence on barnyard grass (Echinochloa crus-galli). Numerous barnyard grass plants with symptoms of smut galls caused by Ustilago trichophora on stems were collected from two counties, víz. Hajdú-Bihar (East-Hungary) and Jász-Nagykun-Szolnok (Middle-Hungary). The infested plants were examined in laboratory, which included the observation of the symptoms and the morphological properties of the spores and the colonies developed from sporidia. The results suggested that this fungus could be effective against barnyard grass weed so the biological control of this weed plant (Echinochloa cruss-galli) can be managed by using Ustilago trichophora biopreparates. As Ustilago trichophora can produce abundant sporidia in liquid culture, a high effectiveness control should be apply by Ustilago trichophora smut fungus as a mycoherbicide in Echinochloa weed control.

  • Mass occurrence of a Phoma-like fungus on common ragweed (Ambrosia artemisiifolia) in Hajdúság region, East Hungary
    55-60
    Views:
    199

    Common ragweed (Ambrosia artemisiifolia L.) is one of the most important, allergenic weed species in Hungary. A. artemisiifolia invades both a broad range of often disturbed areas (brownfields) and either undisturbed ones like waste lands, roadsides, riverbanks and railway tracks. In field crops it can cause considerable yield losses mainly in sunflower, maize, cereals and soybean. In Hungary many inhabitants suffer from allergy caused by Ambrosia pollen which results a serious human-health risk. The aim of the control is to prevent flowering and seed propagation of A. artemisiifolia. Until now the occurrence of numerous pathogenic fungi which attack common ragweed plants have been identified in Hungary, however there is not yet available biological weed control program because of shortage in acceptable effectiveness, and endangering cultural plant species. During our weed surveys in the region of Hajdúság (East-Hungary) we found numerous common ragweed plants showing heavy necrotic lesions on leaves and stems. The objective of this study was to identify the fungus which was isolated from diseased tissues of common ragweed (A. artemisiifolia). The identification of fungus based on morphological characters of colonies and features of conidia and chlamydospores developed on malt extract agar (MEA) plates. After examination of axenic cultures we revealed that the fungus isolated from the leaves ands stems of common ragweed was a Phoma-like species.

  • In memory of József Vörös mycologist (1929–1991) on the 25th anniversary of his death
    33-37
    Views:
    138

    Twenty five years ago died professor József Vörös, internationally recognized mycologist, phytopathologist. On the field of microscopic fungi research, especially on taxonomy of plant pathological conidial fungi (Deuteromycetes, Coelomycetes, Hyphomycetes) gained imperishable reputation during his more than 40 years research activity. As author of 17 fungus taxa and publisher of numerous mycological data J. Vörös took place in the history of mycology.

  • White rust species (Chromista, Peronosporomycetes, Albuginales, Albuginaceae) on common weeds in Hungary
    30-33
    Views:
    194

    The obligate plant parasite fungi in the family Albuginaceae are responsible for causing white rust diseases on weeds and they are rather common worldwide. Weedy plants with characteristic symptoms have been collected in 2014 and 2015 on location Hajdú-Bihar and Jász-Nagykun-Szolnok counties in Hungary. The determination of the species were based on the morphological characters both pathogens and hosts. Albugo candida was determined on shepherd’s purse (Capsella bursa-pastoris). Common purslane (Portulaca oleracea) is a host for Wilsoniana portulacae. The fungus Wilsonia bliti (syn.: Albugo bliti), the causal agent of white rust disease was found on redroot pigweed (Amaranthus retroflexus).

  • Preliminary estimation of the efficacy of Fusarium sporotrichioides Sherb. as biological control agent against common milkweed (Asclepias syriaca L.)
    201-204
    Views:
    242

    A study of fungi responsible for severe leaf spots of common milkweed (Asclepias syriaca L.) in the Hajdúság region (East Hungary), Fusarium sporotrichioides and Alternaria alternata were isolated from infested leaf tissues. F. sporotrichioides was the most virulent fungus in pathogenicity tests conducted on healthy leaves of common milkweed plants. Inoculation of common milkweed (A. syriaca) in different growth stages with F. sporotrichioides yielded similar symptoms as the original ones. Spray mixtures containing 1.0×106 conidia/ml gave effective control when common milkweed plants were sprayed until runoff occurred. Laboratory (wet chamber) and field experiments showed that asexual spores of the fungal pathogen, F. sporotrichioides, exhibited bioherbicidal activity against common milkweed (A. syriaca).

    More efficient control efficacy was observable on elder plants (at flowering stage) than younger ones. These results initiate that this fungus may be a biocontrol agent for controlling this invasive weed but should clarify its hosts because it could infect cultivated plants as well.

  • Characterization of two rust fungi related to biological control concept in Hungary
    195-199
    Views:
    271

    Weeds cause serious problems in agriculture on a global scale. These plants reduce yield and the quality of crops by competing for water, nutrients and sunlight. The improper or excessive usage of herbicides have led to development of resistance in some weed species while contaminating the environment; therefore, biological control has an increasing role as an alternative method for controlling special weed species.

    The aim of this study is to make a brief review of biological control of weeds by pathogens and to characterize two rust fungi (Puccinia lagenophorae and Puccinia xanthii) which are broadly examined recently in a biological control concept and have been found on their hosts, such as common groundsel (Senecio vulgaris L.) and common cocklebur (Xanthium strumarium L.), two common and difficult to manage weeds both in horticultural and agricultural lands also in Hungary.

  • Let's talk about 'One fungus One name'. Changes in the fungal taxonomy
    11-14
    Views:
    203

    Mycologists have recorded a few hundred thousand Latin names for fungi and these are thought to refer to 70 000 or so separate species. The use of molecular techniques in fungal taxonomy and systematics in the last 25 years has provided massive amounts of information to clarify phylogenetic relationships, encouraged grant support, and complicated the jobs of classically-trained mycologists. Taxonomists have a reputation for being traditionalists, but the community has recently embraced the modernization of the nomenclatural rules by discarding the requirement for Latin descriptions, endorsing electronic publication, and ending the dual system of nomenclature, viz. teleomorph and anamorph names, which used parallel for the sexual and asexual phases of pleomorphic species. A group of taxonomists accepted ’The Amsterdam Declaration on Fungal Nomenclature’ and its basic principle the ’One fungus – one name’ has been incorporated in the Code of Nomenclature (’Melbourne Code’) in 2011. The next, and more difficult step will be to develop community standards for sequence-based classification. As the’One fungus – One name’ theory is a brand-new issue for the Hungarian plant doctors and practical specialists, it seems reasonable to review this to promote conversations between generations in Hungarian language.

  • Dieback of apricot plantations caused by 'Ca. Phytoplasma prunorum' in Borsod-Abaúj-Zemplén county (Northern-Hungary)
    34-41
    Views:
    105

    Plant diseases caused by phytoplasmas have increasing importance in all over the world for fruit growers. Lately, phytoplasma diseases occur on many fruit varieties and responsible for serious losses both in quality and quantity of fruit production. In the long-run these diseases cause destruction of fruit trees. The apricot phytoplasma disease (Ca. Phytoplasma prunorum) was first reported in Europe in 1924 from France. In 1992 the disease has also been identified in Hungary. On the base of growers' signals serious damages of "Candidatus Phytoplasma prunorum" Seemüller and Schneider, 2004 (formerly: European stone fruit yellows phytoplasma) could be observed in different stone fruit plantations in the famous apricot-growing area nearby Gönc town, Northern-Hungary. Field examinations have been begun in 2009 in several stone fruit plantations in Borsod-Abaúj-Zemplén County mainly in Gönc region which is one of the most important apricot growing regions in Hungary, named “Gönc Apricot Growing Area”. Our goals were to diagnose the occurrence of Ca. Phytoplasma prunorum on stone fruits (especially on apricot) in the North-Hungarian growing areas by visual diagnostics and confirm data by laboratory PCR-based examinations. All the 28 collected samples were tested in laboratory trials and at 13 samples from apricot, peach, sour cherry and wild plum were confirmed the presence of phytoplasma (ESFY). On the base of observations it seems evident that the notable losses caused by "Ca. Phytoplasma prunorum" is a new plant health problem to manage for fruit growers, especially apricot producers in Hungary. 

  • Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
    188-190
    Views:
    117

    Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.

  • An unusual occurrence of Taphrina deformans on apricot trees in Hungary
    86-92
    Views:
    100

    In 2011 a very rare occurence of leaf curl (leaf blaster) disease symptoms of apricot (pathogen: Taphrina deformans) was observed in different apricot plantations in Eastern-Hungary (Debrecen-Józsa, Hajdú-Bihar county) and Northern-Hungary (Bekecs district, Borsod-Abaúj-Zemplén county). The pathogen is common on peach and nectarine resulting serious symptomps and considerable damages in almost every year, although in this year infections of peaches were very low close to the locations. Although there are some references that apricot and even almond are hosts of the fungus but in Hungarian circumstances the infestation on apricot counts unique and rare. Observations were made on symptoms, microscopic photos and measures were taken on exoasci, ascospores and blastospores, respectively. As far as we know this is the first description of Taphrina deformans fungus on apricot in Hungary.

  • Dr. Oszkár Tóth awarded by „Antal Gulyás medallion for crop protection“ in 2011 (laudation)
    11-15
    Views:
    72

    The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors (Chamber) established a joined Award Committee in September of 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by awarding the „Antal Gulyás medallion for crop protection” which are available for outstanding teachers, researchers, and practical crop protection specialists. The biography of late Antal Gulyás distinguished professor of plant pathology is available in a separate article of this issue. 
    The first person to be decorated with the „Antal Gulyás medallion for crop protection” is Dr Oszkár Tóth retired reader of plant pathology for his excellence in teaching crop protection. Dr Oszkár Tóth had been involved in teaching - by delivering both lectures and practical lessons - crop protection, namely plant pathology in the legal predecessor institute of Debrecen University for more than 30 years where generations of crop protection specialists were encouraged to get a thorough knowledge in plant pathology. The one-time students of the splendid teacher welcome and congratulate to the award, moreover wish him peaceful and happy years.

  • The effect of dissolved oxygen on common carp (Cyprinus carpio) and basil (Ocimum basilicum) in the aquaponics system
    89-96
    Views:
    248

    Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum basilicum).

    Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.

    In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
    2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments. 

  • Phylogenetic analysis of Phoma species
    100-107
    Views:
    92

    The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasites, and saprophyte fungal species. Up to now, the characterization of Phoma species and other taxa of Phoma has been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
    In this study, we tried to find molecular markers which can be used as phylogenetics markers in the molecular based classification in the Phoma genus.
    We employed a part of the translation elongation factor 1 subunit alpha (EF-1α=tef1) containing both introns and exons and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA, as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twelve different Phoma species sequences were analysed together with the closely related Ascochyta ones. The constructed phylogenetic trees, based on tef1 and ITS sequences, do not support the traditional Phoma sections based on morphological characterization. However, we managed to distinguish between the Phoma strains and Ascochyta species by comparing their tef1 sequences through parsimony analysis. We proved that a tef1 can be a useful phylogenetic marker to resolve phylogenetic relationships at species level in Phoma genus.
    Both parsimony sequence analyses confirmed that the Phyllosticta sojicola species is identical to the Phoma exigua var. exigua species as Kövics et al. (1999) claimed. However, the evolutionary distance by ITS sequences within Phoma species is too small to get well based consequences for the phylogenetic relationships of Phoma genus.
    Further investigations would be necessary to clarify whether the tef1 and ITS sequences as phylogenetic molecular markers are well suited for the classification of Phoma species.

  • ”FOR CROP PROTECTION” Establishment medallion in honour of Antal Gulyás (1884-1980)
    5-10
    Views:
    71

    The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors (Chamber) established a joined Award Committee in September of 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by award Antal Gulyás medallion for crop protection“ which are available for outstanding teachers, researchers, and practical plant protectionists. 
    The members of Committee: dr. István Szarukán, president, dr. György Kövics, secretary, dr. István Dávid member (Public Utility), dr. László Kiss, president, dr. Gábor Tarcali, secretary (Chamber). Handing over of medallion, charter and gold badge will be happened generally once a year in a special ceremony. The award was established in commemoration of Antal Gulyás, who was the first eminent professor of plant protection in Debrecen and reached outstanding achievements in the field of teaching of agronomists for more than 30 years, and research on plant pathology. He became one of the first Honoris Causa Doctors of Debrecen Agricultural University in 1978. The Committee made its decision on the first meeting that the first medallion will be award to dr. Oszkár Tóth retired reader of plant pathology for his excellence in teaching plant protection in 2011. His laudation is available in a separate article of this issue. 

  • Phylogenetic studies of Phoma species by maximum likelihood analysis
    37-46
    Views:
    104

    The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
    In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
    The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. 

  • Comparison of the technological background of aquaponic systems
    47-52
    Views:
    251

    Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.

    The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yield and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NON were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).

  • Phylogenetic studies of soybean pathogen Phoma species by Bayesian analysis
    53-61
    Views:
    119

    We carried out phylogenetic study analyzing sequences of genetic markers in the taxonomy of Phoma and Phoma-like fungi. Different species of Phoma and Phoma-like fungi occurring on soybean (Phoma pinodella, Phoma sojicola, Phyllosticta sojicola, Phoma exigua var. exigua) are difficult to identy because of their high morphological and symptomatic similarities.
    Twenty-two isolates of nine different Phoma species were obtained from reference culture collections. Seven of them were isolated from soybean, the others were collected from different hosts.
    The Phoma isolates were firstly characterised by morphologically, and then we employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1), ITS region, as well as β-tubulin partial sequences as potential genetic markers to infer
    phylogenetic relationships among different Phoma species..Finally, their ITS and tef1 sequences were sequenced and analysed by Bayesian approaches.
    According to phylogenetic trees inferred by Bayesian analysis of tef1, ITS and β-tubulin sequences, different Phoma species can be separated proving that these phylogenetic markers are well suited for phylogenetic studies of Phoma species. However, the phylogenetic tree does not support the traditional Phoma sections based on morphological characterization.
    Bayesian analyses of the three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The molecular data provide evidence for reclassification of formerly mentioned soybean pathogens.