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Application of semen evaluation techniques
5-11Views:180Laboratory methods of semen evaluation are used to select males for artificial insemination. The current review describes several techniques that have been recently used for sperm analysis. Conventional microscopic methods in combination with the objective computerassisted sperm motility and morphology analyzers and flow cytometry, allows to obtain more precise information about the membrane and functional status of spermatozoa. By using several methods to detect motility, viability, acrosomal and capacitation status besides DNA integrity sperm biology and some of the mechanism involved in sperm cry injury can be better understood. The number of possible targets related to sperm quality is increasing, and possible that some of them could enable sperm analysis for predicting freezability and fertility to be improved.
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Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
17-20Views:235The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.
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Investigation of intersexual polled goats
11-15Views:149The sex reversal may occur in all mammalian species, but is connected to a favourable trait – the polledness – only in the goat. Later abnormal sex ratio was noticed in these goat populations, in which a part of the phenotypically male individuals was sterile. These males have two X chromosomes. In goats the PIS (Polled Intersex Syndrome) mutation is responsible for the absence of horns in homozygous and heterozygous individuals. This same mutation causes a female-to-male sex reversal, but only in the homozygous polled genetic female goats. The PIS mutation inhibits the expression of the FOXL2 gene which is responsible for ovary development, and a protein encoded by this gene inhibits the activity of the Sox9 gene. The Sox9 gene stimulates the development of the cells of the testis. When the FOXL2 gene is inhibited, the Sox9 gene is activated and transforms the ovary cells into testis cells. In our article we briefly introduce the morphological and chromosome investigations of three intersex individuals we found.
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Investigation of the heat shock gene Hsp70 polymorphism in different sheep genotypes
41-45Views:126Nowadays the climate change has an increasing effect on the animals. The warming climate brings up several problems on the area of the animal husbandry, which ones are really important. From the first time the living beings have defensive mechanisms against the heat shock. In current examination we use– from Hungary and from other countries collected –samples of sheep breeds, which are living on different climate. Our fundamental assumption was, that the animals living on other climate adapted to the changes of the environment and there are differeces in their genetic background. These fixed mutations we are looking for in the HSP70 heat shock gene, but we haven’t found any polymorphism yet. We are going to involve further breeds and more individuals in the investigations.
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Age of puberty and conception rates of Hungarian Merino, (Merino x Somali) F1 and (Merino x Barbados Blackbelly) F1 ewes
15-19Views:163The aim of our examination was to detect the puberty of the Hungarian Merino and its hairsheep crossbred ewes, and also to determine their average conception rates after having ram exposed in May and June. Hormon diagnostation was used to evaluate the exact date of the very first ovulation. Ultrasound technique was used for checking the conception rates of ewes. The pregnancy detection was applied after a month of the date of taking off the ram.
We evaluated the ages of ewes at puberty. Statistical deviation and standard error were calculated. The results proved the Hungarian Merino x Somali (N=10), ((x ± s) = 173 ± 43) and also the Hungarian Merino x Barbados Blackbelly crossbred genotypes (N=7), ((x ± s) = 186 ± 19) have their puberty much earlier as compared to the fullbred Hungarian Merino ewes (N=10), ((x ± s) = 231 ± 95). We experienced the highest conception rate in the group of the (Hungarian Merino x Somali) F1 ewes after exposing the ram in the „out-of season” period of time. -
Effect of the storage temperature onto the quality of the semen of rams of different breeds
75-79Views:114Semen of 10 Tsigai,3 Prolific Merino and 7 Barbados Blackbelly rams was taken in January, out of the season. Split samples of the diluted semen samples were kept at 23, v.s. 8oC and their motilitywas evaluated daily for 3 days by subjective microscopic investigation. The ratio of motile spermatozoa was strongly decreased during the first day, later the motility rate was sinking more slowly. It could be stated by multivariance analysis that the motility value of diluted semen of Prolific Merino rams was higher and showed a slower reduction as compared to the other two breeds. The decrease of the motility rates was slower at 8oC than at 23oC.
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Tenability of ram semen
63-66Views:106It could be stated that the diluted semen of Awassi rams taken in the breeding season preserved its fertilizing abilityat different temperatures for different periods of time. The motility of spermatozoa kept at 23 vs. 8oC was checked daily. The largest spread of data was observed 24 hours after taking the semen, then the motility rate of cells showed a linear decrease. Motility results of fresh and frozen-thawed samples were compared also after heat resistance test and significant differences were found between these groups. Significant individual differences were observedin the sperm motility after heat resistance test.
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Study of the rams’ jumping order
59-62Views:108The bevahiour of Babolna Tetra, Ile de France and Suffolk rams was studied at semen collection by artificial vagina. The sequence of rams coming out of the group to the place of semen collection and the number of jumpings needed to ejaculation were observed. It was investigated if there were changes in these characters at different occasions, and did they show connection to the body condition. The number of jumpings before ejaculation was different in the different breeds. Semen was taken at the first jump from 63% of the Suffolk, 56% of the Babolna Tetra and 55% of the Ile de France rams. There was no early ejaculation at the Ile de France, but it was common among the Suffolk rams. The greatest changes in the jumping order between different semen collections were observed in the Babolna Tetra breed. There was a medium correlation in the Suffolk breed between the jumping order and body condition in the autumn and spring, which was tight and significant in winter.
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Potential use of bamboo in the phytoremediation in of heavy metals: A review
91-97Views:259There are many literature sources focusing on the phytoremediation of woody plants, but there are only few dealing with the phytoremediation of bamboo plants. Phytoremediation technology has the advantages of little disturbance to the environment and low remediation cost. Bamboo mainly exists in tropical and subtropical regions. As an energy plant, bamboo has a fast growth cycle, large biomass, simple cultivation, high economic efficiency, and convenient harvesting, which highlights the advantages of bamboo in phytoremediation. In addition, bamboo plants have good tolerance and uptake ability to heavy metals and have high application potential and development value in uptaking heavy metal contaminated soil. However, due to climate, temperature and other reasons, bamboo cannot be widely planted in most countries. Research status of remediation of heavy metal contaminated soil by bamboo plants is summarized. The feasibility of its application in heavy metal contaminated soil is discussed in this paper. Aiming at the shortcomings of existing research, bamboo plants have a prospect in the field of plant phytoremediation for the future.
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Investigation of directions of crossing in maize (Zea mays L.)
43-48Views:89In Hungary, we examined eight features of twelve direct cross hybrids over a period of three years. The twelve hybrids were derived from direct and reciprocal crosses of four parental lines. We did not find significant differences in the cases of stalk diameter and leaf number in the average of 3 years in any of the hybrids. Statistically explainable differences in primary branches were observed in hybrids UDH5 and UDH8 and also in UDH6 and UDH11. It was evident in both instances that degrees of heterosis in reciprocal crosses far exceeded those of direct ones. High tassel branch number was dominant over the low one; consequently, parents with higher tassel numbers enforced their effects during the formation of this trait in hybrids. We experienced positive correlation (r=0.67**) between plant height and main ear height. A positive correlation (r=0.89**) was also found between the tassel length of the main axis above the lowest and the upper side branch. We observed a medium correlation (r=-0.42**) between the number of primary lateral branches and plant height. The number of primary lateral branches showed the highest heterosis. These results can be utilized in practical selection and seed production.
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Storage of ram semen in gelatin supplemented extender
7-10Views:117The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.
We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05). -
Ovarian performance of Barbados Blackbelly and Dorper crossbred ewes in the mirror of body conditions
23-26Views:75The aim of examination was to detect the puberty and to follow the ovarian cycle of Barbados Blackbelly×Dorper ewes in connection with their body conditions and the effects of meteorological parameters. The blood samples had been taken from 17th of November 2008 untill 08th of June 2009 for analyzing the progesteron concentrations, establishing whether they showed perpetual ovarian performance or not. The end of examination
was timed due to releasing the ram in middle of May 2009.
The daily avarage bodyweight-growth is well featured by the bodyweight tendence. The body conditions were evaluated by ultrasound tissue examination.
During the full length of the examination the avarage daily temperature, the avarage humidity level and also the number of daily sunlighted hours were served by the Agrometeorological Observatory of the University of Debrecen.
The bodyweight tendence and the meteorological parameters were correlated to the breeding season of ewes. In conclusion the bodyweights and their tendences at the young age and the body condition parameters measured at certain anatomical areas can influence the length of the breeding season of the ewes. However the number of daily avarage sunlighted hours, the daily avarage temperature and also the humidity did not effect the cyclic ovarian perfomance of the Barbados Blackbelly×Dorper ewes in Hungary.