Current Issue

No 2 (2020)

Published December 1, 2020



Heterobeltiosis and combining ability among Egyptian cotton genotypes under well irrigated and water deficit conditions

Two field experiments were carried out during the 2014 and 2015 seasons to evaluate certain genotypes of Egyptian cotton under well irrigated and water stress conditions. The cross combination Giza 69 x Australy recorded the best values for better parent heterosis for all physiological measurements and earliness index under well irrigated a...nd deficit conditions. Of the female parents, Giza 86 was found to be a good general combiner for most yield characters under both normal and stress conditions, followed by Giza 94. Data illustrated that Giza 45 was the best general combiner for most fiber quality traits under both conditions. The cross combinations Giza 86 x Dandra and Giza 69 x Pima S6 showed significant positive SCA values for seed cotton and lint yield/plant under well irrigated and water deficit conditions. However, the combinations Giza 77 x PimaS6 and Giza 94 x 10229 recorded significant positive SCA effects for seed cotton yield/plant under stress. Whilst, the cross Giza 68 x 10229 recorded significant desirable SCA effects for most fiber quality properties. In this context, the estimates significant positive general and specific combining ability effects indicated that the epistasis and/or dominance effects for F1 hybrid in cotton could be important to a certain extent.

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Effect of plant growth promoting Rhizobacteria (PGPRS) on yield and quality of processing tomato under water deficiency

Chlorophyll fluorescence was measured of H1015 tomato hybrid with different bacterial treatments (B0–B1–B2–B3) and three irrigation treatments: regular irrigated (RI), deficit irrigated (DI) and non-irrigated conditions (I0). The aim of the experiments was to show the effects of plant growth promoting rhizobacteria on the yield, dry m...atter and vitamin C content of processing tomato during different irrigation treatments, and measuring the chlorophyll fluorescence during the ripening and development stages. According to the results, none of the bacterial treatments had a statistical effect on the quantity and quality of the tomato and on the chlorophyll fluorescence, only the irrigation. Further studies are needed.

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Deproteinized plant juice as part of circular economy: A short review and brief experimental data

As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from... the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.

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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell

The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cher...ry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model.  HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.

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Effect and interaction of crop management factors and crop year on the yield of maize (Zea mays L.)

The aim of this study was to determine the combination of treatment levels of crop management factors which can optimize and sustain maize yield under varying climatic conditions. The effect of winter wheat forecrop, three tillage systems (Mouldboard-MT, Strip-ST, Ripper-RT), two planting densities (60,000 & 80,000 plants ha-1...>), three fertilizer levels (N0-control, N80, N160 kg ha-1) with four replications in irrigated and non-irrigated treatments were evaluated over a five year period, 2015–2019. The obtained results revealed that growing season rainfall positively correlated with yield, whereas, temperature negatively correlated with yield. Impact of adverse weather on yield was less severe in biculture, irrigated plots, at lower planting density (60,000), lower fertilizer rate (N80) and in RT and ST, compared to MT. In years with favorable rainfall, yields of MT and RT were significantly (P<0.05) higher than ST. However, in a less favorable year, such as 2015, with 299 mm growing season rainfall and the lowest July rainfall (59% below mean) there was no significant difference (P>0.05) in yield among the three tillage treatments. Higher planting density (80,000), and fertilization rate (N160) in tandem with MT are treatments combination conducive for high yield under favorable climatic conditions, whereas, in years with low rainfall and high temperatures, RT and ST offer alternative to MT for optimum yield with 60,000 plants ha-1 and N80 treatment level. Crop year effect accounted for 20.7% of yield variance, fertilization 35.8%, forecrop 12.8%, plant density 3.4%, tillage 1.2% and irrigation <1%. It is conclusive that with proper selection of the appropriate levels of agrotechnological inputs the adverse effect of weather on yield can be mitigated.

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Effect of tillage practices, fertilizer treatments and crop rotation on yield of maize (Zea mays L.) hybrids

This research was conducted at the University of Debrecen Látókép Research Station and is part of an ongoing long-term polyfactorial experiment. The impact of three tillage systems (Mouldboard plowing-MT, Strip tillage-ST, Ripper tillage-RT) and two levels of fertilizer treatments (N80 kg ha-1, N160 kg ha...-1) along with a control (N0 kg ha-1) on the yield of maize hybrids (Armagnac- FAO 490 & Loupiac-FAO 380) cultivated in rotation with winter wheat was evaluated during a two-year period (2017–2018).

Amongst the three tillage treatments evaluated, ripper tillage (RT) had the highest average yield (10.14 t ha-1) followed by mouldboard tillage (MT) and strip tillage (ST) with 9.84 and 9.21 t ha-1 respectively. Yield difference between RT and MT was not significant (P>0.05), as compared to ST (P<0.05). Soil moisture content varied significantly with tillage practices and was highest in ST, followed by RT and MT (ST>RT>MT). Yield of RT was 7–9% higher than MT in monoculture plots, while MT reign superior in biculture plots (monoculture: RT>MT>ST; biculture: MT>RT>ST).

A positive interaction between tillage and fertilization was observed, with higher yield variation (CV=40.70) in the non-fertilized (N0) plots, compared to those which received the N80 (CV=19.50) and N160 kg ha-1 (CV=11.59) treatments.

Incremental yield gain from increase fertilizer dosages was significantly higher in monoculture, compared to biculture. There was no significant difference in yield between N160 and N80 in the biculture plots (12.29 vs 12.02 t ha-1). However, in monoculture plots, N160 yield was 23% higher than the N80 kg ha-1 (N160=11.74 vs N80=9.56 t ha-1).

Mean yield of maize in rotation with winter wheat was 28% (2.47 tons) higher than monoculture maize. The greatest benefit of crop rotation was observed in the control plots (N0) with an incremental yield gain of 4.39 tons ha-1 over monculture maize (9.92 vs 5.43 t ha-1).

Yield increased with higher fertilizer dosages in irrigated plots. Fertilizer application greatly increased the yield of maize and accounted for 48.9% of yield variances. The highest yield (11.92 t ha-1) was obtained with N160 kg ha-1 treatment, followed by N80 kg ha-1 (10.38 t ha-1) and N0 kg ha-1 (6.89 t ha-1) respectively.

Overall mean yield difference between the two hybrids was not statistically significant, however, yield of FAO 380 was 3.9% higher (9.06 vs. 8.72 t ha-1) than FAO 490 in monoculture plots, while in biculture plots, FAO 490 was 4.1% higher than FAO 380.

Average yield in 2018 was 13.6% (1.24 t ha-1) higher than 2017 for the same set of agrotechnical inputs, thus, highlighting the significant effect of cropyear.

Armagnac (FAO 490) cultivated in rotation with winter wheat, under ripper tillage and N80 kg ha-1 is the best combination of treatments for optimum yield.

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Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)

 In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to im...prove immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.

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The role of cultivar susceptibility and vineyard age in GTD: examples from the Carpathian Basin

Grapevine trunk diseases (GTDs) are among the most severe problems in viticulture worldwide. The exact etiology and the role of endophytic microorganisms is not known yet and there is no adequate protection or curative treatment against the disease. Hungarian wine regions are also affected by the disease, and there is restricted information... about the rate of infection nation-wide and about the susceptibility of the Hungarian cultivars.

The main objectives of our research are to measure the symptom expression and the damage caused by GTDs, to understand the epidemiology and etiology of the disease to establish a foundation of a proper disease management.

Cultivar susceptibility groups were created with the aim to allocate some Hungarian cultivars and the role of vineyard age was also examined in symptom expression.

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Examination of different fungicides against Macrophomina phaseolina in laboratory conditions

In Hungary, sunflower is the third most important arable crop, which has a lot of pathogenic fungi. One of these fungi is the Macrophomina phaseolina, which is a well-known fungus in all over the world, since this pathogen has more than 700 host plants. In Hungary, several host plants can be found as well. The M. phaseolina produces microsc...lerotia, which can survive in the soil and residues for almost 10 years. For now, there is no efficient treatment against this pathogen because of this fungus, since it is extremely resistant and cannot be destroyed easily. The only effective treatment against the fungus is genetic defence. In this study, three different fungicides were tested in vitro against the fungus. The Mirage (prochloraz) seemed to be the most effective fungicide as it completely arrested the hyphal growth. In contrast, the Amistar Xtra (azoxystrobin and ciprochonazol) has only a minor effect on the growth of M. phaseolina. Thirdly, the Retengo (pyrachlostrobin) arrested the hyhpal growth of the fungus with 71% at 100 ppm, in other words, the use of this fungicide seems promising. 

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Genetic progress in winter wheat quality and quantity parameters

Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-cro...p were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.

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Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment

The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.

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The significance of local meteorological stations in research planning

The goal of research planning is to optimize human and material resources while maximizing efficiency. If there are databases that can be used as a substitute for own data collection, effective research can be facilitated by reallocating resources. In the case of environmental studies, the knowledge of the climatic conditions of the study p...eriod is known to be a key factor in research planning process.

 In the present study, the data of our meteorological station in our research area (known as “Szamárháti Tanya”, Kesznyéten, Hungary) was compared with the measurements of meteorological stations operated by the competent water authority. Stations were taken into the study within a 10 and 20 km radius over a period of 21 months, to determine which provided more relevant data from the area.

During the evaluation of results, the relationship between the local and regional weather stations were determined, and deductions were made from the obtained results to support the decision which of the targeted investigations could be of greater benefit. 

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Flesh firmness examination of scab-resistant apple varieties in a storage experiment

Of the different physical characteristics of fruits, one of the most essential qualities is flesh firmness, as is an important parameter when selling fresh apples, processing the fruit (processing industry) and during its storage. In the scope of our research, we examined five apple varieties originating from the Derecske horticultural site... of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The five examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The two-month storage experiment was conducted at 16-17 °C, which had an aggressive effect on our stored apples. Flesh firmness, weight and diameter were measured on three dates for each of the five apple varieties. Our results were evaluated using IBM SPSS Statistics 20 software. We aimed to compare flesh firmness, weight and diameter of the five apple varieties and their changes during storage. Isaaq (86.34 N/cm2), Modí (94.06 N/cm2) and Fujion (84.90 N/cm2) had outstanding initial flesh firmness results. The Modí apple variety provided the best storage parameters.

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Angle-count sampling method for estimating forest stand volume – a practical approach

Point sampling, which is also known as angle-count sampling (ACS), can be considered an efficient way of estimating the basal area and volume of forest stands. It is possible to use it in forest management: providing more accurate estimates (precision <10%) of  site and stand characteristics needed for management planning. 20 black (Robinina pseudoacacia L.) stands were selected at final cutting age to determine the regeneration criteria based on their total volume. It was verified that at P=5% there was no difference between the main volume values of stands indicated in the relevant forest plans as well as calculated by the ACS method.


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The impact of cultivar and irrigation on yield, leaf surface temperature and SPAD readings of chili pepper

Paprika is an extremely popular and widespread plant species in Hungary. Chili peppers belongs to paprika and it can be consumed fresh or processed. The production of peppers began with the cultivation and control of some parameters for good results of paprika production. The aim of this article is to compare the water supply of four chili ...pepper hybrids ("Habanero", "Hetényi Parázs", "Unikal" and "Unijol") by means of investigation on open field and to examine how different temperatures and water stress factors influence the yield of chili peppers. The experiment area consisted of four different hybrids in three different irrigation treatments (control, deficit- and regular irrigation). The chlorophyll content was estimated with SPAD and the leaf surface temperature with Raytek MX4 TD. Our research concluded that deficit irrigation produced the highest yields for all cultivars and the lowest yields for the highest water supply.

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Effect of hail net on the water potential of an apple orchard

 Apple production has seen a decline in yields in recent years, primarily due to ecological factors such as drought, water stress, water scarcity, uneven rainfall distribution, frost damage and hail damage. Ecological factors that are harmful to the plant can be eliminated by human intervention, irrigation or the ...use of hail net. In our study, we investigated the positive effects of hail net on the development of plant water potential as the vegetation progressed, in non-irrigation area with temperature and humidity. Water potential values were determined using a field osmometer. Confirmed the positive effect of the ice protection net in the apple orchard of Early Gold and Golden Reinders. Our results were supported by statistical analyzes our results.

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Measurement of degradation on under-utilized natural turf

The role of turf serving animal husbandry is significantly declining with the decreasing number of grazing livestock in Hungary. Accordingly, the area of under-utilized or non-utilized turfs is increasing. At the University of Debrecen, Institutes for Agricultural Research and Educational Farm, Karcag Research Institute we studied four type...s of turf utilization in three repetitions on a salt meadow with Alopecurus pratensis. As a result of the performed examinations, we identified the composition of the flora structure on the investigated area and we measured carbon-dioxide circulation and soil moisture.

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Comparison of the geranium (Pelargonium) pathological results of 2016-2017

The research was carried out in a Gyenes Flower gardening between 2016 and 2017 in Kecskemét. The gardening was founded in 1978. Initially, the main plants were gerbera (Gerbera) and yucca (Yucca), later replaced by the geranium (Pelargonium) cultivation as a result of market demand. In horticulture, there are about than 80 variety geraniu...m of the standing, running, semi-trailer types and English gnawing. The Pelargonium had different sizes and colors. The study was set up in 1,000–1,000 pieces of geraniums each year. The following pathogens have damaged the geranium stock: Botrytis cinerea, Pythium debaryanum, with a rare occurrence of Alternaria porri, Phytophthora cryptogea. The greatest destruction was caused by botrytis (Botrytis cinerea). In the first experimental year, 42% of the 1,000 geraniums tested were infected with fungal diseases (30% B. cinerea, 8% P. debaryanum, 4% other fungi). In 2017, fungal infections were detected on 380 geraniums in the 1,000 tested geraniums (290 Botrytis cinerea, 70 Pythium and 20 other fungal diseases). In addition to the use of fungicides, we increased the spatial position of geraniums, early irrigation and frequent ventilation to ensure successful control. By 2017, we were able to reduce the damage caused by pathogens by 4 percent.

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Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)

 Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and y...ield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.

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Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen

The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in u...ndeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.

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