Articles

Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary

Published:
2024-06-03
Authors
View
Keywords
License

Copyright (c) 2024 by the Author(s)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How To Cite
Selected Style: APA
Zegeye, Z. B., Magyar, K. ., & Novotni-Dankó, G. (2024). Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary. Acta Agraria Debreceniensis, 1, 193-200. https://doi.org/10.34101/actaagrar/1/13797
Abstract

The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.

References
  1. Abd-Allah, S.; Abd-El Rahman, H.H.; Shoukry, M.M.; Mohamed, M.I.; Fatma M. Salman; Abedo, A.A. (2019): Some body measurements as a management tool for Shami goats raised in subtropical areas in Egypt. Bull Natl Res Cent 43, 17. https://doi.org/10.1186/s42269-019-0042-9
  2. Aké-Villanueva, J.R.; Aké-López, J.R.; Segura-Correa, J.C.; Magaña-Monforte, J.G.; Aké- Villanueva, N.Y. (2017): Factors affecting conception rate of hair ewes after laparoscopic insemination with chilled semen under tropical conditions. Small Ruminant Res. 153, 114–117. https://doi.org/10.1016/j.smallrumres.2017.06.006
  3. Al-Thuwaini, T.M. (2021): The relationship of hematological parameters with adaptation and reproduction in sheep; A review study. Iraqi Journal of Veterinary Sciences, 35 (3): 575–580. DOI:10.33899/IJVS.2020.127253.1490
  4. Alvarez, M.; Chamorro, C.A.; Kaabi, M.; Anel-López, L.; Boixo, J.C.; Anel, E.; Anel, L.; de Paz, P. (2012): Design and "in vivo" evaluation of two adapted catheters for intrauterine transcervical insemination in sheep. Anim Reprod Sci.;131(3–4):153–9. doi: 10.1016/j.anireprosci.2012.03.001. Epub 2012 Mar 10. PMID: 22483334
  5. Anakkul, N.; Suwimonteerabutr, J.; Tharasanit, T.; Panyaboriban, S.; Khunmanee, S.; Thanomsuksinchai, N.; Techakumphu1, M. (2013): “Production of Black Goat Using Laparoscopic Artificial Insemination and Embryo Transfer”. The Thai Journal of Veterinary Medicine 43 (2):259–63. https://he01.tci-thaijo.org/index.php/tjvm/article/view/9650.
  6. Anel, L.; de Paz, P.; Alvarez, M.; Chamorro, C.A.; Boixo, J.C.; Manso, A.; Gonzalez, M.; Kaabi, M.; Anel, E. (2003): Field and in vitro assay of three methods for freezing ram semen. Theriogenology 60, 1293–1308. https://doi.org/10.1016/S0093-691X(03)00140-7
  7. Anel, L.; Kaabi, M.; Abroug, B.; Alvarez, M.; Anel, E.; Boixo, J.C.; De Paz, P. (2005): Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes a field assay. Theriogenology. 63: 1235–1247. DOI: 10.1016/j.theriogenology.2004.07.001
  8. Beam, S.W.; Butler, W.R. (2019): Effects of energy balance on follicular development and first ovulation in postpartum dairy cows. Journal of reproduction and fertility. Supplement 54 411–24. DOI:10.1530/BIOSCIPROCS.4.032
  9. Bergstein-Galant, T.G.; Weiss, R.R.; Kozickl, L.E. (2018): Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep. Reproduction in Domestic Animals, v. 54, n. 2, p. 401–407. DOI: 10.1111/rda.13381
  10. Casali, R.; Pinczak, A.; Cuadro, F.; et al. (2017): Semen deposition by cervical, transcervical and intrauterine route for fixed-time artificial insemination (FTAI) in the ewe. Theriogenology, v. 103, p. 30–35. DOI: 10.1016/j.theriogenology.2017.07.021
  11. El-Badry, D.A.; Amil, Z.L.; Mona, H.S. (2014): Studies on laparoscopic intrauterine insemination of barki ewes (using different insemination doses) as compared with cervical. Assiut Veterinary Medicine Journal, v. 60, n. 142, p. 172–178. DOI: 10.21608/avmj.2014.171004
  12. Evans, G.; Maxwell, W.M.C. (1987): Salamons' artificial insemination of sheep and goats. No. Ed. 2 pp. xi + 194 pp.: (Butterworths: Sydney.Austria)
  13. Faigl, V.; Vass, N.; Jávor, A.; Kulcsár, M.; Solti, L. (2012): Artificial insemination of small ruminants-a review. Acta Veterinaria Hungarica. 60: 115–129. DOI: 10.1556/AVet.2012.010
  14. Farrag, B. (2019): Productive characteristics and reproductive responses to estrus synchronization and flushing in Abou-Delik ewes grazing in Arid Rangelands in Halaieb-Shalateen-Abouramad Triangle of Egypt. World’s Veterinary Journal, 9 (3): 201–210. https://dx.doi.org/10.36380/scil.2019.wvj26
  15. Fukui, Y.; Roberts, E. (1978): Further studies on non-surgical intrauterine technique for artificial insemination in the ewe. Theriogenology.10: 381–393. https://doi.org/10.1016/0093-691X(78)90042-0
  16. Fukui, Y.;Kohno, H.; Okabe, K.; Katsuki, S.;Yoshizawa M. (2010): Factors affecting the fertility of ewes after intrauterine insemination with frozen thawed semen during non-breeding season. Journal of Reprod Develop. 56: 460–466. DOI: 10.1262/jrd.10-015t
  17. Geenty, K.G.; Brien, F.D.; Hinch, G.N.; Dobos, R.C.; Refshauge, G.; McCaskil M.; Ball, A.J.; Behrendt, R.; Gore, K.P.; Savage, D.; Harden, S.; Hocking-Edwards, J.E.; Hart, K.; van der Werf, J.H.J. (2014): Reproductive performance in the Sheep CRC Information Nucleus using artificial insemination across different sheep production environments in southern Australia. Animal Production Science, 54(6):715–726. DOI: 10.1071/AN11323
  18. Gordon, I. (1999): Controlled Reproduction in Sheep and Goat. CAB International, ISBN:0851991157 page 450.
  19. Halbert, G.W.; Dobson, H.; Walton, J.S.; Sharpe, P.; Buckrell, B.C. (1990): Field evaluation of a technique for trans-cervical intrauterine insemination of ewes. Theriogenology 33:1231–1243. https://doi.org/10.1016/0093-691X(90)90041-Q
  20. Hristova, T.; Stoycheva, S.; Maslev, T.; Ralchev, I. (2011): The influence of the time of implementation of PMSG on some of the reproductive parameters in sheep with synchronized oestrus. Biotechnology in Animal Husbandry, 27(4), 1845–1850. DOI: 10.2298/BAH1104845H
  21. Ibarra, D.; Laborde, D.; Van Lier, E. (2000): Repeatability and relationship with field mating performance of a serving capacity pen test in rams. Small Ruminant Research, 37 (1–2): 165–169. https://doi.org/10.1016/S0921-4488(99)00135-2
  22. Jiménez-Rabadán, P.; Morrell, J.M.; Johannisson, A.; Ramón, M.; García-Álvarez, O.; Maroto-Morales, A.; Alvaro-García, P.J.; Pérez-Guzmán, M.D.; Fernández-Santos, M.R.; Garde, J.J.; Soler, A.J. (2012): Single layer centrifugation (SLC) improves sperm quality of cryopreserved Blanca-Celtibérica buck semen. Anim Reprod Sci. 2012 Dec; 136 (1–2):47–54. doi: 10.1016/j.anireprosci.2012.09.012.
  23. Kaabi, M.; Alvarez, M.; Anel, E.; Chamorro, C.A.; Boixo, J.C.; De- Paz, P.; Anel, L. (2006): Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix: a postmortem study. Theriogenology. 45. 6–13. DOI: 10.1016/j.theriogenology.2006.04.039
  24. Karagiannidis, A.; Varsakeli, S.; Karatzas, G.; Brozos, C. (2001): Effect of time of artificial insemination on fertility of progestegen and PMSG treated indigenous Greek ewes, during non-breeding season, Small Ruminant Research, 39. 67–71. DOI: 10.1016/s0921-4488(00)00170-x
  25. Killeen, l.D.; Caffery, G.J. (1982): Uterine insemination of ewes with the aid of a laparoscope. Aust. Vet. J. 59, 95. DOI: 10.1111/j.1751-0813. 1982.tb02737.x
  26. Madan, M.L. (2005): Animal biotechnology: applications and economic implications in developing countries. Rev Sci Tech. 24(1): 127–139. PMID: 16110883
  27. Magyar, K.; Veress, L.; Tasi, Zs.; Pécsi, T.; Babik, S.; Horváth, I. (1999): Zootechnical and genetic aspects of a prolific merino program Act. Vet. Hung. 47. 17–31. DOI: 10.1556/AVet.47.1999.1.2
  28. Márton, A.; Faigl, V.; Keresztes, M.; Kulcsár, M.; Nagy, S.; Fébel, H.; Novotni Dankó, G.; Magyar, K.; Husvéth, F.; Solti, L.; Cseh, S.; Huszenicza, Gy. (2009): Milk progesterone profiles, blood metabolites, metabolic hormones and pregnancy rates in Awassi ewes treated by gestagen + eCG at the early breeding season. Vet. Med. Cz, 11.: 507–516. DOI:10.17221/20/2009-VETMED.
  29. Maxwell, W.M.C. (1984): Current problems and future potential of artificial insemination programmes. In 'Reproduction in Sheep'. (Eds D. R. Lindsay and D. T. Pearce.) pp. 291–8. (Camb. Univ. Press.)
  30. Mazinani, M.; Rude; B. (2020): Population, world production and quality of sheep and goat products. Am. J. Anim. Vet. Sci., 15(4): 291–299. DOI: https://doi.org/10.3844/ajavsp.2020.291.299
  31. Moses, D.; Martínez, A.G.; Iorio, G.; Valcárcel, A.; Ham, A.; Pessi, H.; Castañoñ, R.; Marciá, A.; de las Heras, M.A. (1997): A largescale program in laparoscopic intrauterine insemination with frozen-thawed semen in Australian Merino sheep in Argentine Patagonia. Theriogenology 48, 651–657. https://doi.org/10.1016/S0093-691X(97)00281-1
  32. Nagy, P.; Solti, L.; Kulcsar, M.; Reiczigel, J.; Huszenicza, Gy.; Abavary-Mihaly, K.; Wofling, A. (1998): Progesterone determination in equine plasma using different immunoassays. Acta Veterinaria Hungarica, 46(4), 501–513. PMID: 9713151
  33. Naqvi, S.; Joshi, A.; Bag, S. (1998): Cervical penetration and trans-cervical AI of tropical sheep at natural estrus using frozen-thawed ram semen. Small Ruminant Research, 29: 329–333.
  34. Novotniné Dankó, G. (2003). Some Practical and Biotechnological Methods for Improving Reproduction Traits in Sheep. Acta Agraria Debreceniensis, (11), 15–20. https://doi.org/10.34101/actaagrar/11/3437
  35. Palacin, I.; Yániz, J.L.; Fantova-Blasco, E.M.E.; Quintín-Casorrán, F.J.; Sevilla-Mur, E. (2012): Factors affecting fertility aftercervical insemination with cooled semen in meat sheep. AnimReprod Sci. 2012 Jun; 132(3–4):139–44. doi: 10.1016/j.anireprosci.2012.05.005.
  36. Park, J.E.; Graham, J.K. (1992): Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38, 209–222. https://doi.org/10.1016/0093-691X(92)90231-F
  37. Perkins, N.R.; Hill, J.R.; Pedrana, R.G. (1996): Laparoscopic insemination of frozen-thawed semen into one or both uterine horns without regard to ovulation site in synchronized Merino ewes, Theriogenology, 46. 541–5. DOI: 10.1016/0093-691x(96)00175-6
  38. Salamon, S.; Maxwell, W.M.C. (2000): Storage of ram semen. Anim Reprod Sci. 2000 Aug 18; 62(1–3):77–111. doi: 10.1016/s0378-4320(00)00155-x.
  39. Sousa, C.J.H.; MacDougall, C.; Campbell, B.K.; McNeilly, A.S. (2001): The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenic receptor type 1 B (BMPR1B) gene. J Endocrinol, 169(2): R1-6. doi: 10.1677/joe.0.169r001.
  40. Watson, A.J.; Westhusin, M.E.; Winger, Q.A. (1999): IGF paracrine and autocrine interactions between conceptus and oviduct. J. Repr.Fert. 54 pp:303–315.
  41. Windsor, D.P. (1997): Mitochondrial function and ram sperm fertility. Reprod Fertil Dev. 9(3):279–84. doi: 10.1071/r96109.
  42. Yavarifard, R.; Hossein-Zadeh, N.G.; Shadparvar, A.A. (2015): Estimation of genetic parameters for reproductive traits in Mehraban sheep. Czech J. Anim. Sci., 60 (6): 281–288. doi: 10.17221/8242-CJAS
  43. Zegeye, Z.B.; Vass, N.; Tomano, A. (2020): Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen. Acta Agrar. Debr., 2:133–138. doi: 10.34101/ACTAAGRAR/2/7113. https://ojs.lib.unideb.hu/actaagrar/article/view/7113