No. 75 (2018)
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Articles
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Andrological examination of Hungarian Large White and Landrace boars
5-10Views:247The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.
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Investment funding programs on dairy farms in Hajdú-Bihar County
11-16Views:185The dairy farming sector needs constant modernization of its buildings, machines and technology in order to maintain its competitiveness and productivity. For the greater scale investments, these companies try to apply for state funds in addition to their own financial sources. Therefore, the investment support system plays a great role by improving the competitiveness and modernity of these farms. In the last 10 years, 7 funding programs were available for dairy farms. In this research, 25 dairy farms were interviewed in Hajdú-Bihar County. The examined farms cover more than 50% of the milk production in Hajdú-Bihar County. The survey questions focused on the investment funding program use of farms between 2007 and 2017 and renewable energy investments including all programs. The results show how many tenders were submitted, how many were successfully evaluated and completed. All farms had at least two successfully evaluated projects. A correlation was found between completed projects by farm size and completed projects. The connection of the modernity index of dairy farms and the completed projects was also shown.
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A growth model to predict body weight and body composition of broilers
17-24Views:360Models predicting the nutrient partitioning and animal performance have been developed for decades. Nowadays, growth models are used in practical animal nutrition, and they have particular importance in precision livestock farming. The aim of the present study was to introduce a broiler model and to provide examples on model application. The model predicts protein and fat deposition as well as the body weight of an individual broiler chicken from digestible nutrient intake over time. Feed intake (FI) and the digestible nutrient content of the feed are inputs as well as some animal factors like: initial BW, feed intake at 1 and 2 kg of BW, precocity and mean protein deposition. The protein and energy metabolism is represented as in the classical nutrient partitioning models. The protein deposition (PD) is driven by digestible amino acid supply and is under “genetic control”, the so-called potential PD limits the actual PD if protein is oversupplied.
The authors discuss how the model can be used to simulate the animal response upon different scenarios. Examples are given to show that the diet might be limiting if some animal trait is changed. Applicability of the model has shown through running the model by using different feed strategies (three- vs five-phase-feeding) and variations with animal factors. In conclusion, growth models are useful tools to support decision making for defining the most suitable feeds used in a broiler farm. The model presented in this paper shows a high sensibility and flexibility to test different scenarios. By challenging the model with different inputs, the animal response in terms of changes in body weight and feed conversion can be understood more by studying the shift in deposition of chemical constituents. The examples provided in the present paper shows the benefit of using mathematical models and their applicability in precision nutrition. It can be concluded that the growth model helps to apply “from desired feed to desired food” concept.
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The effect of compost made of sheep manure on the first cut of a semi-natural grassland
25-29Views:251In an experiment, two types of compost were tested on natural grassland in order to improve the productivity of a natural sward on solonetz soil. Both composts were made of on-farm produced sheep manure, but the second one was enriched in phosphorus. These fertilizers/composts are officially authorized and can be applied in organic farming. Zero application and three rates of fertilizer were tested (10 t ha-1, 20 t ha-1, 30 t ha-1) on 30 m2 experimental plots wit four replications. Dry matter, crude protein and net energy content for maintenance were measured and determined and their yields per unit area were calculated. We found that all the treated plots produced significantly higher yield than the control ones, and in some cases the yields were almost three times higher. However, the optimal compost dose varied, depending on the examined parameter. Based on the evaluation of the experiment results along with rainfall data, it was concluded that, with higher precipitation, the positive effect of the compost application was bigger. The difference between the natural and enriched compost is remarkable, but it has to be mentioned that even the natural compost showed good results; therefore, it is also effective in improving the fertility of grassland.
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Testing laboratory parameters of compost tea
31-36Views:511During the industrial production of broiler chicken, a large amount of manure is produced, of which easily contained nitrogen content (without pre-treatment) is released into the atmosphere as an air pollutant. In our experiments, we aimed to prepare compost tea, also known as water extract of compost, from pre-treated poultry manure in order to create a product can be utilized as liquid nutrient supply. The poultry manure source was the Baromfi-Coop Ltd. located in Nyírjákó, Hungary, where it was treated by composting. As a result of this pre-treatment of the poultry manure, its nutrient parameters improve and nitrogen is present in a form that is better utilized for plants. Furthermore, this product is suitable for further utilization and also can be the base material for a brand-new product. For this reason the effects of compost/water ratio, incubation time, low oxygen level, and extraction time on the parameters of the resulted product were studied in the frame of developing new soil-life enhancing microbial product, so-called compost tea.
Chemical parameters of the compost used as base material strongly determined the properties of the resulted compost tea, especially the ratio of the various nitrogen forms, their concentration and the salt content. It was found that adding water at a higher rate that means 1/40 and 1/50 mixing ratios results in more cost-effective production. In the experiment the compost tea were held under oxygen-poor conditions, therefore pH of the extractions decreased, which influenced the quality and quantity of their nutrient content.
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Influence of 17-alpha methyl testosterone on the production parameters of common carp (Cyprinus carpio L.) fry
37-43Views:274During our research we aimed at finding an answer as to what extent the different concentrations of 17-alpha methyl testosterone incorporated in the diet of common carp fries can influence the production parameters of the species, as well as how efficient their sexreversal can be with the use of this method. To this end, an aquarium experiment was conducted in the course of which four different hormone treatments were set and monitored. The fish feed was enriched with 17-alpha methyl testosterone in 50 ppm, 75 ppm, 100 ppm, 500 ppm dosages.
The obtained figures revealed that the hormone treatments had no influence on the production parameters and conservation of the common carp fries. Further on, our team is to determine the sex of the fish through the examination of gonads during autopsy when they reach the 500 g average weight.
Furthermore, a male specific test method which was supposed to be of great help in our attempt to select the sex-reversed specimens in the subsequent processes was also put to the trial. During the experiment the DNA-isolation of different sample types (muscle tissue, fin, mucus) of common carp with identified sex was successfully carried out. The extracted PCR product was examined with agarose gel. Our results indicated that the ccmf2 marker was applicable, however, the obtained figures were not reliable.
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The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:215Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
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The effect of drought and cropping system on the yield and yield components of maize (Zea mays L.)
51-53Views:141Different Cropping Systems have many advantages and ensure better crop growth and yielding. Its combination with other agronomic measures can ensure optimal crop density for maximum crop growth and photosynthesis efficiency. The aim of this study was to investigate the influence of different cropping systems on monoculture and biculture rotations [maize- wheat]. The study found that crop rotation does not have a significant effect on the grain nutrition quality, Leaf Area Index (LAI) and Normalized Difference Vegetative Index (NDVI) but has a significant effect on the Soil-Plant Analysis Development (SPAD). Yield and yield components were significantly influenced by crop rotation in this study as yield, plant height, cob weight and number of grains per row all recorded lower mean at 5% probability levels.
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Effect of different weaning time on the growth and survival ofCommon carp (Cyprinus carpio L.) larvae
55-59Views:413Within our experiment, we studied the combined effect of live food application and feed training on the production parameters of carp larvae. In addition, we examined whether there is a difference in the growth rate and survival rate of the specimens of Szeged mirror landrace reared in similar stocking density but with different treatments.
Specimens of Szeged mirror landrace from induced propagation were placed into 12 pieces of 40-liter aquaria. For the 4 different treatments, (K, A3, A6, A9), live food (Artemia salina) was fed, as well as feed training was applied.
We also sought to find an answer as to how our feeding and rearing conditions can affect the production parameters of common carp fries and what impact these conditions may have on their survival rates. At the end of the 2-week experiment we determined the individual weight gain of the carp fries, their survival rate and the biomass growth. The purpose of the study is to examine the optimum timing of shifting from live food to feed for carp larvae.
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HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:311Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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The effect of supplemental pig milk replacer on carbohydrate metabolism – a review
67-71Views:260The aim of this review is to examine the effect of milk supplementation on pigs’ carbohydrate metabolism. These parameters regulate the growth of piglets, the nutrient intake and the period of pre-weaning. Due to the increased litter size, the sow milk yield reaches an individual maximum. The individual maximum has not increased proportionally with the number of piglets born alive. The use of liquid milk supplementation may give the opportunity to keep these large litters. The examined hormones are insulin, insulin-like growth factor, growth hormone, growth hormone releasing hormone, ghrelin and leptin. This review seeks to find these hormones’ interactions.
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Microbiological status of bulk tank milk and different flavored gomolya cheeses produced by a milk producing and processing plant
73-78Views:294The microbiological quality of milk is important not only for food safety, but it can also influence the quality of dairy products. In this study, our aim was to assess the microbiological status of the bulk milk of a milk-producing farm, and some natural and flavored (garlic, dill, onion) gomolya cheeses made from pasteurized milk produced by their own processing plant. We determined the number of coliform bacteria, Escherichia coli, Staphylococcus aureus, and molds of three milk and eight cheese samples. The tests were conducted between July and September, 2017.
In bulk milk, the mean coliform count was 3.83±0.17 log10 CFU/ml; the mean E. coli count was 1.38±0.14 log10 CFU/ml; the mean mold count was 3.74±1.30 log10 CFU/ml; and the S. aureus count was <1.00 log10 CFU/ml, respectively. The mean coliform count in gomolya cheeses was 3.69±1.00 log10 CFU/g; the mean E. coli count was 2.63±0.58 log10 CFU/g; the mean S. aureus count was 3.69±1.35 log10 CFU/g and the mean mold count was 1.74±0.37 log10 CFU/g. The amount of coliforms detected in different flavored gomolya cheeses were significantly different (P<0.05). More than 10 CFU/g of E. coli was found only in the dill flavored cheeses, and S. aureus was found only in dill (3.66±1.86 log10 CFU/g) and onion (3.71±0.52 log10 CFU/g) flavored gomolya cheeses. Based on the obtained results, it was found that the amount of coliform bacteria and E. coli in bulk milk exceeded the limit set in regulation of the Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.) and the amount of S. aureus was below the limit. For gomolya cheeses, the S. aureus count exceeded the limit. The amount of coliform bacteria remained above the limit in cheeses, except for the garlic flavored gomolya cheese. In cheeses, a larger E. coli count was detected than in the bulk milk, but there is no specific limit for cheeses in the regulation. The mold count exceeded the limit specified in the regulation in cheeses, but a lower value was detected relative to milk.
The results show that, in the case of bulk milk and gomolya cheeses, certain detected quantities exceeded the limit values set forth in regulation of MoH 4/1998 (XI. 11.). The results indicate an inadequate microbiological state of the raw material and the finished products. The reasons for these are due to reduced technological hygiene or the inappropriate handling of raw material and finished products. In this study, we have summarized the results of our preliminary studies, which can provide a basis for further hygiene studies.
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Single nucleotide polymorphism analysis in meat-production related genes in broiler chickens
79-82Views:186In broiler chickens, the intensive selection for growth rate, feed efficiency, body composition (breast muscle weight) traits in the last decades was successful. To improve economically important characteristics, it is possible to use molecular markers associated with meat production traits. The aim of this study was to examine genotype polymorphisms in ROSS 308 broilers for thyroid hormone responsive Spot14α, insulinlike growth factor 1 (IGF1), IGF-binding protein 2 (IGFBP2), somatostatin (SST) and prolactin (PRL) genes. A further goal of this investigation was to study the relationship between the polymorphisms and phenotypic characteristics.
In the investigated broiler population, the frequency for CC homozygous genotype was 0.77 in Spot14α (AY568628), AA homozygous genotype was 0.80 in IGF1 (M74176), GG homozygous genotype was 0.85 in IGFBP2 (U15086), DD homozygous genotype was 0.60 in PRL (FJ663023 or FJ434669). Only the AA homozygous genotype was found in SST (X60191). Chickens with AC genotype in Spot14α, and with GG genotype in IGFBP2 had higher body weight (BW) and carcass weight (CW), compared to CC and GT genotypes. However, the differences were not significant (P>0.05). There was significant association (P<0.05) between PRL genotypes and body and carcass weight, where chicken with homozygous DD surpassed individuals with homozygous II genotypes.
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The effect of the queen's age on the Varroa mite (Varroa destructor) burden of honey bee (Apis mellifera L.) colonies
83-87Views:316An apiary trial was conducted in 2016 August to October in Szabolcs-Szatmár-Bereg County, Nyírmada to evaluate the influence of queen’s age on the Varroa destructor-burden in the treatment colonies. Sixty colonies of bees belonging to the subspecies Apis mellifera carnica pannonica in Hunor loading hives (with 10 frames in the brood chamber/deep super) were used. The colonies were treated with amitraz and the organophosphate pesticide coumaphos active ingredients. The amitraz treatment includes 6 weeks. The coumaphos treatment with Destructor 3.2% can be used for both diagnosis and treatment of Varroasis. For diagnosis, one treatment is sufficient. For control, two treatments at an interval of seven days are required. The colonies were grouped by the age of the queen: 20 colonies with one-year-old, 20 colonies with two-year-old and 20 colonies with three-year-old queen. The mite mortality of different groups was compared. The number of fallen mites was counted at the white bottom boards. The examination of spring growth of honey bee colonies has become necessary due to the judgement of efficiency of closing treatment. The data was recorded seven times between 16th March 2017 and 19th May 2017.
Data on fallen mites were subjected to one-way analysis of variance (ANOVA) and Post-Hoc Tukey-test. Statistical analysis was performed using the software of IBM SPSS (version 21.). During the first two weeks after treatments, the number of fallen mites was significantly higher in the older queen’s colonies (Year 2014). The total mite mortality after amitraz treatment in the younger queen’s colonies was lower (P<0.05) compared to the three-year-old queen’s colonies. According to Takács and Oláh (2016) although the mitemortality tendency, after the coumaphos (closing) treatment in colonies which have Year 2014 queen showed the highest rate, considering the mite-burden the colonies belongs to the average infected category. The colonial maintenance ability of three-year-old queen cannot be judged based on the influencing effect on the mite-burden. The importance of the replacement of the queen was judged by the combined effect of several factors.
During the spring-growth study (16th March–19th May) was experienced in the three-year-old queen’s colonies the number of brood frames significantly lower compared to the one- and two-year-old queen’s colonies. In the study of 17th April and 19th May each of the three queen-year-groups were varied. Therefore in the beekeeping season at different times were determined the colonial maintenance ability of queens by more factors: efficiency of closing treatment in early spring, the spring-growth of bee colonies, the time of population shift (in current study, this time was identical in each queen-year), honey production (from black locust).
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Evaluation of population parameters and biometric data of an Eurasian Collared Dove (Streptopelia decaocto Friv.) population in the Great Plain of Hungary
89-92Views:367The Eurasian Collared Dove shows different patterns in density all over Hungary. In some cases, the population has decreased to a lower level, but there are habitats where the population remained stable. Several factors were taken into consideration during the evaluation of this phenomenon. These publications state that the increased urbanization of corvids, the lack of food sources, and the changes in nesting sites caused the decrease in numbers. During our research, we intend to contrast the differences in population structure of Collared Doves that differ from each other in the density of predators presented at nesting sites. As a first step, in this paper, numerous samples (n=118) were taken from a population that is less influenced by corvid species’ nest predation. Our prediction was that the population parameters remained at the level of 1980’s, in which the population of Eurasian Collared Dove has reached its maximum, according to further publications.
During the evaluation process, our data were collected from hunting bags near Nyíregyháza. Results were compared with former publications. We stated that the population structure, such as age and gender distribution remained stable in the evaluated population. In addition, morphometric factors of the collected individuals were also measured, such as weight, wing length, 3rd primary feather, and tail length, which were compared with former surveys. During statistical tests no significant difference was revealed among population parameters, but there were significant differences between most of the morphometric data, (p>0.05).
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The applicability of 10 ml cryotubes for sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus)
93-97Views:251In our study, the comparison of 5 ml straw and 10 ml cryotube during sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus) was carried out. Three different dilution ratios (1:1, 1:4 and 1:9) were also tested using the cryotube. A significantly higher pMOT was recorded using the cryotube in comparison with the straw. VCL and STR were similar in both groups. Cryopreservation had a negative effect on pMOT and VCl using the cryotube and also the straw where, STR was not reduced significantly. An increasing tendency was observed using higher dilution of sperm during cryopreservation; however, significant difference was not recorded between the three groups. VCL and STR were similar in all groups. Cryotube was applicable for the sperm cryopreservation of the Hungarian carp landrace. However, the standardization for the freezing method specified for this volume is recommended. The different dilution ratios needed be tested also during fertilization.
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The effect of feeding different glycerol sources on the performance of lactating sows
99-103Views:189Glycerol is a by-product of the biodiesel industry and it might be a good alternative to moderate the energy deficiency of sows during the lactation period. Preliminary experiments were carried out to test the effect of a powder, solid based “food grade” glycerol source with 72.9% glycerol content (Trial 1) and a liquid “feed grade” glycerol source with 86% glycerol content (Trial 2) on the performance of lactating sows and their litters. Trial 1 was conducted with 5 Hungarian Large White×Hungarian Landrace sows/treatment (313±24.9 kg) and Trial 2 with 12–12 DanAvl (323±17.0 kg) sows and their litters/treatment. Neither the solid, powder based glycerol (Trial 1), nor the liquid glycerol source (Trial 2) had significant effect on the feed intake, reduction in live weight and back-fat thickness, and weaning-tooestrus interval (p>0.05) of lactating sows. In Trial 2, on the 14th, 21st and 27th days of lactation the milk samples were collected and it was found that 50 kg/t glycerol decreased the protein content of milk samples (p<0.05). Glycerol supplementation had no effect on dry matter, fat, lactose content of milk samples (p>0.05). In Trial 2, no significant difference was found between control and experimental sow groups in triglyceride concentration of blood samples and in the activity of liver enzymes (ALT, AST, GGT; p>0.05), but the concentration of plasma glucose and cholesterol increased tendentiously (p<0.10).
Based on our preliminary results, it can be concluded that additional dose trials are needed to perform in order to study the effect of glycerol supplement on milk production and on metabolic processes of lactating sows.
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Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
105-112Views:333Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
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