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Factors affecting the efficiency of superovulation and embryo transfer. Season and hormonal background
73-76Views:644Success of the embryo transfer programs is affected by many factors (breed, type, season, treatment). We assume, that periferical blood level of some metabolic hormones (IGF-1, thyroid hormones, leptin, insulin) affects the success of superovulation and ET. According to our results, there is a
significant difference between the in and out of season periferial blood level of IGF-1 and insulin. Furthermore, decrease in the periferic blood levels of IGF-1 and insulin is less drastic in the pregnant recipients. -
Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary
193-200Views:340The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.
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The effect of supplemental pig milk replacer on carbohydrate metabolism – a review
67-71Views:540The aim of this review is to examine the effect of milk supplementation on pigs’ carbohydrate metabolism. These parameters regulate the growth of piglets, the nutrient intake and the period of pre-weaning. Due to the increased litter size, the sow milk yield reaches an individual maximum. The individual maximum has not increased proportionally with the number of piglets born alive. The use of liquid milk supplementation may give the opportunity to keep these large litters. The examined hormones are insulin, insulin-like growth factor, growth hormone, growth hormone releasing hormone, ghrelin and leptin. This review seeks to find these hormones’ interactions.
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Single nucleotide polymorphism analysis in meat-production related genes in broiler chickens
79-82Views:307In broiler chickens, the intensive selection for growth rate, feed efficiency, body composition (breast muscle weight) traits in the last decades was successful. To improve economically important characteristics, it is possible to use molecular markers associated with meat production traits. The aim of this study was to examine genotype polymorphisms in ROSS 308 broilers for thyroid hormone responsive Spot14α, insulinlike growth factor 1 (IGF1), IGF-binding protein 2 (IGFBP2), somatostatin (SST) and prolactin (PRL) genes. A further goal of this investigation was to study the relationship between the polymorphisms and phenotypic characteristics.
In the investigated broiler population, the frequency for CC homozygous genotype was 0.77 in Spot14α (AY568628), AA homozygous genotype was 0.80 in IGF1 (M74176), GG homozygous genotype was 0.85 in IGFBP2 (U15086), DD homozygous genotype was 0.60 in PRL (FJ663023 or FJ434669). Only the AA homozygous genotype was found in SST (X60191). Chickens with AC genotype in Spot14α, and with GG genotype in IGFBP2 had higher body weight (BW) and carcass weight (CW), compared to CC and GT genotypes. However, the differences were not significant (P>0.05). There was significant association (P<0.05) between PRL genotypes and body and carcass weight, where chicken with homozygous DD surpassed individuals with homozygous II genotypes.