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Performance of sweet cherry cultivars grafted on Colt rootstock
7-10.Views:984In this paper growing characteristics and fruit bearing parameters of ‘Lapins’, ‘Kordia’, ‘Sweetheart’ and ‘Regina’ sweet cherry cultivars grafted on vigorous Colt rootstock were evaluated at the University of Debrecen, Pallag Experimental Station. Based on our data five years old trees can be described with homogenous strong growing, but very week yielding (2.1-3.1 kg/tree), as while fruit size varies between 26.2 and 27.2 mm. Producing the examined cultivars on Colt rootstock with high plant density requires higher attention and more interventions (root pruning, sawing the trunk) during the technology.
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Apple allergens as affected by cultivation technolog and variental factors
91-93.Views:1115Our objective was to study the effect of organic farming and area of cultivation on the allergen content of apple varieties by quantification of the major allergens (Mal d 1 and Mal d 2) using allergen-specific ELISA. The obtained results showed that except Idared IX. 29. most of examined varieties, when cultivated under organic farming conditions, contained lower level of Mal d1 allergen than those produced with integrated farming. The different varieties were found to vary substantially in theirMal d 1 allergen content. Furthermore, with some varieties the area of cultivation affected to some extent the content of Mal d 1 allergen.
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The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon
46-49.Views:372Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.
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Content of mineral- and sulphur compounds in Hungarian and French garlic varieties
61-65.Views:399In our days about 2 million tons of garlic are grown in the world. Garlic was used as medicine already in ancient Egypt. It contains approx 33 sulfur compounds, several enzymes, 17 amino acids and minerals. We determined the content of diallyl sulfone, boron, calcium, copper, iron, potassium, magnesium, natrium, phosphorus and zinc in fi ve garlic varieties (3 French, 2 Hungarian). We searched if there were signiffi cant differences between the varieties.
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The effect of modified bacterial virulence to host-pathogen relationship (Phaseolus vulgaris L. Pseudomonas savastanoi pv. phaseolicola)
53-56.Views:550The Pseudomonas savastanoi pv. phaseolicola is one of the most expressive biogen stressors of the bean (Phaseolus vulgaris L.) in Hungary. The chemical and agrotechnological defence is inefficient, so breeding is the only workable way. The conventional cultivars are susceptible to PS while most of the new industrial varieties have genetic resistance to the pathogen. The genetic background of resistance is, however, a complex system in the bean. Leaf resistance is a monogenic system, but this gene is not expressed in juvenile stage of the host. The pathogen species can be divided into different races. After inoculation with virulent strains, typical symptoms appeared on the leaves. To understand the details of host-pathogen relationships, there were carried out experiments using bacterial strains with altered virulence. Six transposon mutants of the PS were tested. Our main objective was to test these modified bacterial strains on bean cultivars of known genetic background. First we analysed the symptoms, and then the correlation between the symptoms and the multiplication of mutant bacteria. Three cultivars (Cherokee, Inka and Főnix) were tested.
The infection by the virulent PS isolate produced typical symptoms on the three cultivars tested. Mutant bacteria (except strain 756) did not cause any significant symptoms on the hosts. The mutant 756 induced visible symptoms on the cultivars Cherokee and Inka. On Cherokee there were small watersoaked lesions, and HR (hypersensitivity reaction) was detected on Inka, but this was restricted to some cells only (mikro HR). The rate of multiplication of the wild type strain was much higher than the multiplication of the mutants. Bacteria were detected in the cotyledons and primordial leaf, but there is not any substantial number of bacteria in leaves, except for strains 757, 1212 and 1213. The rate of multiplication of strain 756 was intermediate. These, and other experiments can help to understand the genetic background of resistance and the host-pathogen relationship in the Pseudomonas-bean pathosystem.
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The effect of storage to optical properties in case of two apple fruit varieties
33-36.Views:910The keeping quality of fruits is a very important factor in storage point of view, since it could be elongated the consumption time of fruits. In many cases the storage may be necessary, e.g. in short time it is not able to market due to the rich harvest, or all fruits could sell in markets throughout the year. The keeping quality of various fruit species is greatly different; moreover it could be observed some differences between the varieties as well. In our research, we have investigated the spectral properties of the external surface and fl esh of two ripe apple varieties – Jonagored and Granny Smith – before and after storage (about 3°C and about three weeks). The hyperspectral investigation was carried out with AvaSpec-2048 Fiber Optic Spectrometer. The experimental results represented the differences between healthy and chilling injured apple fruits. Furthermore, some indices were created to given countenance to the spectral differences.
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Antioxidant capacity, total phenolics and mineral element contents in fruits of Hungarian sour cherry cultivars
59-64.Views:1213Several epidemiological studies revealed that the consumption of antioxidant compounds and the risk of atherosclerosis, increased blood pressure or cancer are inversely proportional. Fruits of sour cherry contain a wide range of antioxidant compounds including melatonin, perillyl alcohol, ellagic acid, several flavonoids, polyphenolics, and anthocyanins. This study was carried out to survey the antioxidant power and mineral element content of seven commercial sour cherry cultivars and three cultivar candidates and to assess the influence of some external conditions on fruits' functional properties. Our analysis revealed nearly 5- and 2-fold differences between the lowest and highest antioxidant capacities and total phenolics content, respectively. Some cultivars (`Kántorjánosi' and ‘Újfehértói fürtös') and cultivar candidates (D, 'Petri' and 'Éva') showed outstanding antioxidant capacity and total phenolic content; in addition, mineral element content in fruits of the ‘Újfehértói fürtös' cultivar was also favourable. Redox parameters of fruits were influenced by the cultivation plot or fruit positions within the canopy in about half of the cultivars tested. Genetic background of cultivars forms the decisive factor in determining fruits' antioxidant capacity, although external factors may have also sizeable modifying effects. Enhanced functional properties of the fruit may also be further increased through breeding programs since considerable variation exists within the tested germplasm.
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Stress physiology of palm trees II. The effect of heavy metals and high irradiance on the photosynthesis of palm Trachycarpus fortunei
84-88.Views:285A study was carried out to analyse the individual and combined effects of heavy metal toxicity and high irradiance on the photosynthetic characteristics of young, fully expanded leaves of palm seedling Trachycarpus fortunei under laboratory conditions. Heavy metals were found to inhibit both the light and dark reactions of photosynthesis and the inhibition was more affected in the light than in the dark. Single photoinhibitory conditions caused a 60 % decrease in the electron transport activity after 120 min of light exposure which was completely reversible in the dark. In contrast, the combined effect of high light and heavy metal treatment resulted in a 90 % decrease in the activity, but no reversible recovery in the dark could be detected. This indicated that the simultaneous effect of these two stress factors led to irreversible damages of the photosynthetic machinery and as a consequence caused the general destruction of the plant.
Abbreviations and symbols: Fo: initial chlorophyll fluorescence; Fm: maximum total fluorescence; Fv: variable fluorescence; AFi: intermediate level of fluorescence induction; PSII: photosystem 2.
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Change of Thiamine concentration and amino acid composition during sparkling wine base Production
62-66.Views:281It has been established, that thiamin content added in 3 mg/I concentration to musts before fermentation resulted in the enhanced storability of sparkling wine base at lower SO2 levels. Fermentation rate is not increased by this concentration and it causes no "thiamine taste" in organoleptic evaluation.
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Determination of quality in stored pear fruits by chemical analysis and sensorial judgement
27-31.Views:823Aim of this research/project to determine the external and internal preferences of pear using descriptive sensory analysis, consumer preference. The research was performed on local consumers. Destructive measurements included fi rmness by puncture tests, soluble solids content (SSC), titrated acidity (TA). While there is a general positive trend for increasing preference with fi rmness, some consumers will prefer softer apples, and some will dislike the fi rmest pear. Sensorial judgement is able to classify the attractiveness, preference of properties specifi c for pear. Tests were performed by 13 persons on 4 pear varieties (Bosc kobak, Conference, Packham’s Triumph, Dessertnaia) checking 11 sensorial properties on a scale of 1 to 100 points. Relations of measurements and judgements were processed by correlation analysis. For analysis, the objects were furnished in 2011 from different growing sites (Csenger, Mérk and Nagykanizsa), taking from the store (in January) immediately. Among those the best notes were given to Bosc kobak and Conference coming from Mérk and to Packham’s Triumph grown at Nagykanizsa. It was stated that the success of sensorial judgement depends on the state of maturity, which is diffi cult to guarantee to be synchronous among samples of different varieties. Mature fruits are more praised as a sample of Bosc kobak taken from a chain of department store proved to be of balanced composition regarding its sugar/acid ratio (0.12) and the optimal fi rmness (5.75 N/cm2). Results of the correlation analysis suggest that the thickness of the skin is a decisive component of preference (r= 0.857), the typical pear flavour (r= 0.948), the taste as sweetness and acidity (r= 0.930 and r= 0.813). At the same time, no valuable relation could be detected between the data raised in the laboratory and the preference expressed by the sensorial tests, which should signalise that the opinion of consumers does not depend on any individual parameter obtained in the laboratory (sugar- or acid content, fi rmness) but rather on the complexity of several decisive components together (sweetness, acidity, fl avour, skin, etc.). The consumers’ preference cannot be measured objectively without the aid of adequate expertise and a prosperous surrounding, samples of optimal maturity and a thoughtfully edited judging form.
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Investigation of the Antioxidant Effect of Hungarian White Wines and Tokaj Wine Specialities
22-26.Views:507Authors investigated the antioxidant effect of wine-phenolics, expressed as total antioxidant status (TAS-value). This property may play an important role in elimination of the harmful effects of free radicals in human body.
Independently of their polyphenol composition, special quality Tokaj wines did not show extremely high TAS-values. In Tokaj Aszu wines the total antioxidant status was independent of the "puttony" number but was influenced by the length of aging time.
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Investigation of flowering dynamics of the basil (Ocimum basilicum L.) and its consequences in production
74-80.Views:471The flowering dynamics of Ocimum basilicum L., (a common population maintained at the UHFI) was studied with the aim to create an exact and practically applicable method for definition of phenological phases.
According to our observation the development of individual flowers of the basil can be characterized by 8 distinguishable phases, which must be considered for description of the actual phenological stage of a spike.
An accurate model was created for the unambiguous description of flowering process of different flowers within the spike and for the individual plant as a whole. The new flowering index formula is calculated from the number of flowers weighed by their phenological phases.
The time dependence of flowering is presented by functions fitted to values of the flowering index. The results reflected different patterns of the main inflorescence, the inflorescences formed on the side shoots in the first or in the second half of the flowering period. However, for description of the flowering process of the whole plant, a sigmoid function proved to be the appropriate model.
The accumulation process of the essential oil could be characterized by the flowering index values. They showed close correlation (r=0.964) at high probability.
The new method assures an exact definition of phenological phases in basil. Its application seems to be optimal during breeding procedures, seed production and even in production of high quality drugs, according to the requirements of the Good Agricultural Practice (Guidelines for GAP, 1998).
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Preliminary data on attractiveness of phenylacetaldehyde-based lures on economically important plant bug pests (Hemiptera: Miridae)
87-94Views:739Several plant bug species (Miridae) are important pests of crops and vegetables, thus monitoring them is of essential importance for effective pest control. During the current, preliminary study synthetic plant volatile combinations were tested in field conditions in Hungary in alfalfa fields. Beside semiochemical baited traps, sweep-netting was also performed. In the experiments three plant bug species were found in higher numbers: Adelphocoris lineolatus, Lygus rugulipennis and L. pratensis. As a novel, interesting finding L. pratensis was attracted to phenylacetaldehyde baited traps. For all species, both males and females were trapped in all combinations. Sweep-netting and semiochemical baited traps showed different efficacy in case of the three species, as sweep-netting catches were highly biased for A. lineolatus, which indicates the higher efficacy of this method as compared to the tested semiochemical-baited traps. On the other hand, semiochemical baited showed better performance for L. rugulipennis and L. pratensis. For these species none of the tested combinations performed better than phenylacetaldehyde baited traps. The potential implication of results in view of monitoring are discussed.
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Factors affecting apricot fruit antioxidant capacity and mineral element contents
95-99.Views:528Several epidemiological studies revealed that the consumption of antioxidant compounds and the risk of atherosclerosis, increased blood pressure or cancer are inversely proportional. The individual amounts and relative proportions of macro- and micro elements present in food is also of great consideration since these are involved in a wide range of physiological processes including the influence of the redox homeostasis. The antioxidant power and mineral nutrient content of fruits might be affected by several factors including genotype, ripening stage, year-effects or a wide range of environmental conditions. This study was carried out to survey the antioxidant power and mineral element content in fresh fruits of apricot and analyse some genetic and environmental factors that may have important contribution to the inner content of apricot fruits. In addition, the influencing effect of the extraction procedure used for antioxidant analyses was also tested. Our analyses indicate that a considerable fraction of antioxidant capacity is attributable to the hydrophilic antioxidants. The genetic background has crucial importance in determining apricot fruit antioxidant capacity and mineral nutrient content; however, the growing season and the ripening time of fruits may have also important effects.
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Effect of maturity stage on content, color and quality of tomato (Lycopersicon lycopersicum (L.) Karsten) fruit
41-44.Views:761Soluble solids (Brix°), carbohydrate, organic acid, lycopene, polyphenols and HMF content of indeterminate round type tomato Lemance F1 fruits were measured in six ripeness stages from mature green to deep red stage. Color of fruits was determined by CIELab system. The L*, a*, b* values were received directly and used to calculate from which the a*/b* and the chroma were calculated. The Brix', carbohydrate, lycopene and HMF content were the highest in the 6111 stake (deep red). Carbohydrate contents constitute nearly 50% of the Brix°. The mature green stage had the lowest acid content but in subsequent stages it was fundamentally unchanged. Polyphenol content changed little during fruit ripening. Lycopene content changed significantly during maturation and accumulated mainly in the deep red stage. Analyses showed that a*/b* was closely correlated with lycopene and can be used to characterize stages of maturity in fresh tomatoes.
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Knot formation by Pseudomonas syringae subsp. savastanoi on the in vitro shoots of Sorbus redliana
59-62.Views:513Two strains of Pseudomonas syringae subsp. savastanoi were isolated from Forsythia sp. and Nerium oleander in Hungary in 1997. The effects of growth regulators produced by the bacteria were studied in different experiments. The strains were co-cultured with Sorbus redliana in vitro shoots without being in contact with the plant on solid media. Further culture filtrates in different concentrations were added to the culture medium. The growth regulators presented in the agar caused knot formation on the shoots and on the leaves in both kinds of culture. There were significant differences in the cultural and physiological characters, auxin and cytokinin activity of the strains of different origin.
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Composition and storage of pear cultivars from Nagykanizsa
63-68.Views:1039The composition of five pear varieties (‘Abate Fètel’, ‘Bosc’, ‘Williams’, ‘Conference’, ‘Packham’s Triumph’) grown in Nagykanizsa was investigated in three consecutive years (2008, 2009, 2010). A storage experiment was performed in 2008. Four winter pear cultivars were kept in an ULO store for four months and their parameters measured after two and four months. The parameters tested were: size, weight, water soluble solids, titratable acidity, glucose, fructose, sucrose, water soluble pectin, total polyphenols, free radical scavenging capacity, copper and zinc content. The fruits of ‘Conference’ and ‘Bosc’ varieties were found to contain the highest sucrose and total sugar content, while ‘Abate Fétel’ had the lowest sucrose and highest glucose levels among cultivars tested. ‘Williams’ pear was the most acidic. Brix, total sugar, sucrose and water soluble pectin were decreased during storage. Titratable acidity slightly decreased in fruits of Conference pear. Polyphenols and free radical scavenging capacity did not show a significant change during storage.
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Vegetative parameters of apple cultivars in integrated and organic production systems
15-18.Views:478Success of apple production is highly influenced by the applied production system and the planted cultivar. In this paper growing characteristics of 39 apple cultivars were studied in integrated and organic production systems. These kind of parameters are less studied in the cultivar and training system examinations, although they have huge effect on the training and maintaining of canopy, on the pruning necessity, ultimately on the production costs. According to our results the thickness of the central axis of apple trees showed significant differences between the integrated and the organic systems. Axis of the trees with lower trunk thickness tapers more slightly in the integrated production system, than in the case of the trees with thicker trunk in the organic system. Thicker axis is not accompanied by thicker trunk, namely the thickness of the central leader starts to decrease stronger in the organic production system, compared to the integrated one.
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Influence of strawberry and raspberry consumption on the antioxidant status of human body
109-113.Views:479Several parameters influencing the antioxidant capacity were measured in our experiments to compare strawberry and raspberry cultivars. The analysed cultivars were: 'Honeoye', 'Elsanta', 'Onebor (strawberries) and 'Glen Ample', Fertődi zamatos', 'Mailing Expoit' (raspberries). Besides the determination of dry matter content the content of glucose, fructose, total phenol and anthocyanine, the reducing power, H-donating activity and total scavenger capacity were measured as well as the compound of mineral nutrients. Among the species and the cultivars, differences were obtained. These results call the attention to the features of cultivars and to count on the antioxidant-capacity describing values in case of consuming these fruits. In early spring and summer the consumption of strawberry and raspberry has a beneficial contribution to the antioxidant status of our body. In this respect these are more valuable than the year-long consumable apple. Accordingly, our measurements showed higher amount of sucrose, phenolic compounds and anthocyanine in raspberries, but strawberries contained more glucose and fructose and had stronger H-donating activity. It is worth to keep in mind that e.g. which cultivar contains more of certain compounds because of the strictly determined allowable dose in some diseases (e.g. diabetes).
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Growth and yield of sweet cherry trees on different roostocks
98-101.Views:467The first nine years' results of sweet cherry rootstock trial from Hungary are presented with the aim to select efficient rootstocks for the local ecological conditions. The trials were established in 1989 with 'Van' and 'Germersdorfi óriás' cultivars on the following rootstocks: mahaleb Sainte Lucie 64, Colt, MxM 14, MxM 97. All the trees were headed at 80 cm and trained to a modified Brunner-spindle system. Tree size and yield was measured every year, and the cumulative yield efficiency was calculated.
Based upon the results, mahaleb cherry SL 64 is a vigorous rootstock with good compatibility and productivity. In comparison to SL 64. the trunk cross-sectional area and canopy spread of the 'Van' trees decreased by 10-15% on rootstock Colt, while the trees of 'Germersdorfi orias' on Colt roostock grew even larger than on SL 64. Considerable 30-40 % reduction of tree size was achieved on trees grafted on M x M hybrids. Trees of `Germersdorfi óriás' showed a similar tendency without significant differences. The cumulative yield efficiency of `Van' trees after nine years was highest on rootstock MxM 14, followed by Sainte Lucie 64. Trees on Colt and MxM 97 rootstocks showed low productivity. `Germersdorfi óriás' produced the highest cumulative yield efficiency on SL 64, followed by MxM 14 and Colt and last MxM 97. Biennial bearing index of heavy cropping 'Van' trees was smaller than that of `Germersdorfi óriás'. The trees of 'Van' on MxM 97 showed higher biennial bearing index, while 'Germersdorfi orias' on different rootstocks showed similar tendency, but without significant differences.
MxM 14 and MxM 97 rootstocks reduced the crotch angle of the shoots on both sweet cherry varieties which is disadvantegous to spindle training. Slight suckering (1-3/year) of the rootstocks Colt, MxM 14 and MxM 97 were observed during the first few years.
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Role of wholesale markets in fresh fruit and vegetable distribution in Hungary
46-50.Views:239The primer function of fruit and vegetable wholesale markets — which was the physical concentration of supply and demand previously — is changing gradually as consumer habits change and logistics functions (e.g. product manipulation, redistribution without the physical presence of the product) become more important with the technical development. Mainly individual growers sell their product to retailers at the Hungarian regional producers' markets and the Budapest Wholesale market on NagykOrosi Street, which is the largest wholesale market in Hungary regarding area, produce arrival and turnover. Parallel to the establishment of Producers' Organisations (P0) their market presence should be urged in selling their produce and also in ownership — and decision-making procedure — of the markets. The produce auction system and the on-line sales experiences of New Jersey State (USA) can contribute to the investigation of development possibilities and realization of development of Hungarian wholesale markets.
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Selenium enriched vegetables as biofortification alternative for alleviating micronutrient malnutrition
75-81.Views:884There is a very difficult equation for malnutrition and over-consumption. That means malnutrition even of vitamins and/or minerals (Ca, Cu, Fe, I, Mg, Se and Zn, vitamin A) affects more than two billion people worldwide, largely due to low concentrations or poor bioavailability of the nutrients in the diet. In some developed countries in contrast, over-consumption, particularly of over-refined cerealbased foods, has contributed to the development of an epidemic of metabolic diseases. So, producing nutritious and safe foods sufficiently and sustainably is important target at the same time challenge of modern agriculture. In the past, great efforts have focused only on increasing crop yields, but enhancing the concentrations of mineral micronutrients has become an urgent task. The main daily food source is the staple crops specially in developing countries of the world, i.e., wheat, rice, cassava, beans, sweet potato or maize. These kind of plants are often deficient in some of mineral elements. Thus, the increasing of bioavailable concentration of micronutrients in edible crop tissues (via biofortification) has become a promising strategy in modern agriculture, providing more nutritious foods, to more people, with the use of fewer lands. Biofortification of these trace elements can be achieved application with agronomic process such as soil or foliar fertilization or crop breeding even conventional technic and/or genetic engineering. This review highlight progress to date and identify challenges faced in delivering biofortified vegetable crops as well as the agronomic approaches and tools to improve crop yield and micronutrient content of food crops.
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Preliminary biochemical studies on a model growing of different tobacco plant (Nicotiana tabacum L.) cultivars
83-87.Views:240The aim of our preliminary studies was to characterize the change in the activity of enzymes PPO and PDX, the concentration of total soluble phenolic compounds and soluble protein content of different tobacco cultivars measured in tobacco plants during cultivation. The results suggest that a shorter cultivation period (13-14 weeks) is more favourable for tobacco plants as protein source than for tobacco industrial use (16-17 weeks). We found some correlation between the concentration of total soluble phenolic compounds and PPO activity data. In the increasing and maximum period of total phenol content the PPO activity was high. But later because of decrease of substrate phenol content the activity of PPO also decreased. We found higher soluble protein concentrations in Virginia than in Burley varieties.
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Effect of the environmentally friendly production systems on the vegetative characteristics of apple cultivars
11-14.Views:578In this study effect of the environmentally friendly production systems on the brunch cross sectional area of apple trees trained to free spindle canopy are presented based on the data of 39 cultivars. According to our results branch thickness of the apple cultivars located in the lower, middle and upper region of the tree are more homogenous in the integrated production system compared to the organic one. Therefore the severity of the annual maintain pruning must be necessarily more moderate in the integrated growing system, than in the organic production system.
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Growing characteristics of apple cultivars and canopies
7-10.Views:717In our study we aimed to evaluate the growing characteristics of new prospective apple cultivars and their canopies. The following parameters were measured: I) thickness of the trunk and the central leader, II) ratio of the trunk thickness and the basic branches (Zahn indexes), III) number of the branches of the central leader, and iv) thickness of the branches of the central leader. The main branches of the trunk and the central leader tend to over thickening in the case of cvs ‘Red Idared’, ‘Wilton’s Red Jonaprince’ and ‘Jeromine’. The density of the branches of certain parts of the canopy was too low for cvs ‘Crimson Crisp’, ‘Wilton’s Red Jonaprince’, ‘Fuji September Wonder’ and ‘Red Idared’. The density of the branches of certain parts of the canopy was too high for cvs ‘Gala Venus Fengal’, ‘Wilton’s Red Jonaprince’ and ‘Red Cap Valtod’. Relationship was found between the tapering dynamics of the central axis and the thickness of the main branches, which was stronger in super spindle canopies than in slender spindle crown forms.
