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In vitro investigation of King Oyster Mushroom [Pleurotus eryngii (DC.: Fr.) Quel.] strains in vegetative growing phases
47-53.Views:324The king oyster mushroom ( Pleurotus eryngii) is more and more popular amongst the producers due to its excellent taste and relatively easy cultivation . In the course of our work we collected 15 king oyster mushroom strains from various habitats in Hungary in order to get a better picture about the growth of the vegetative mycelia of the species and its different strains. In the in vitro experiments we investigated the growth of the strains at various temperatures and pH. incubated them in light and darkness and in aerobic and anaerobic atmosphere. In addition to these we measured the weight of dry mycelia produced in a given time by the strains. Our results showed that the above mentioned environmental conditions resulted in a very different growth rate of the vegetative mycelia of the various P. eryngii strains. These results may provide valuable data about the vegetative phase of the cultivation.
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Comparison of light yellow fleshed pepper varieties grown on rockwool under unheated forcing conditions
108-110.Views:180Experiments are going on all over the world assisting the joint effort of researchers and practicing specialists to identify the methods which can help either in the reduction of production costs or in the increase of yields.
The task of the growers is to make profitable use of the forcing facilities and to satisfy market demands at an acceptable price by means of improving production technology and applying new scientific, technological and technical information.
For the last few years, rockwool based forcing has been gaining in importance. The subject of our scientific work was the analysis of an important question of this technological variant, the selection of the variety. Besides, we also tried to identify the most suitable pruning technology for the varieties studied.
In Hungary, the highest demand is commonly known to be for the light yellow fleshed varieties which are suitable for stuffed dishes. In the future, due to their special quality and appearance, as well as to the Hungaricum character, they could become important export goods on the European Union market. It was within this variety type that comparison between varieties already common in production (HO F1, HRF F1, Danubia F1) was carried out, trying to get an answer to the question which of the three varieties could be produced with the greatest success. Considering the quantitative and qualitative indicators, it was H6 F1 that proved the best out of the three varieties tested under unheated forcing on rockwool. It excelled the other two varieties both in quality and in average fruit weight, preserving this advantage until the end of the growing period.
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Effects of farmyard manure (FYM) and blended NPSB fertilizer rate on growth and yield of potato (Solanum tuberosum L.) in Gewata, Kaffa, southwest Ethiopia
14-22.Views:285Potato (Solanum tuberosum L.) is an essential crop for nutrition and food security and generating income to small-holder farmers in Ethiopia. Productivity of the crop is, however, limited by poor nutrient management practices and low soil fertility. In most parts of potato growing areas of Ethiopia, soil are deficient in macro (nitrogen, phosphorus and sulfur) and micro nutrients (boron) which are known to affect production and productivity of potato. A field trail was accompanied to determine the growing response of potatoes ’Belete varieties’ to combine the treatment of farmyard manure and blended NPSB (nitrogen, phosphorous, sulfur and boron) fertilizer at Gewata, southwest Ethiopia during 2019/2020 main cropping season. The variables are laid out four level of farmyard manure (0, 5, 10 and 15 t/ha) and four level of blended NPSB fertilizer (0, 50, 100, and 150 kg/ha). The treatments were organized in a completely randomized block design with three replications using a 4x4 factorial layout. SAS (version 9.4) was used for data were analyzed. The finding indicate that, combined application of FYM (farmyard manure) with NPSB significantly (p<0.01) affects plant height, average mass of tuber, number of marketable and unmarketable tuber per hill, and tuber yields per plot. It also significantly (p<0.05) influenced days to flowering, maturity, and tuber number per hill. The highest tuber weight (99.33 g), tuber number per hill (9.67), and yield (34.10 kg) per plot were recorded from the mixed fertilization of 15 t FYM with 150 kg/ha NPSB mineral nutrients. As a current finding, it can be stated that the combined use of FYM (15 t/ha) and NPSB blended fertilizer could improve potato growth and yield in the study area (150 kg/ha).
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Grapevine and apple replant disease in Hungary
57-61.Views:226Field experiment was conducted to study the replant problems of grapevine and apple. Plantings were in three different fields: on virgin soil, on apple replant soil and on vine replant soil. Each field was planted with 60 pieces of grafted vine (variety Bianca on rootstock Berl. X Rip. T.K. 5BB) and 60 pieces of grafted apple (variety Gloster on rootstock MM. 106). Fungicide (BUVICID K with 50 % captan agent, 0.5 g/1 1 soil) and nematocide (VYDATE 10 G with 10 % oxamil agent, 0.03 g/1 I soil) treatments were used in the soil in order to identify the causal factor of the problem.
Biological soil test was conducted to test 17 soil samples of II wine districts and vine growing fields in plastic pots, under shading net. No root pieces were left in the soil. Two bud-cuttings of the Bed. X Rip. T 5C rootstock varieties were used as test plants. In each case, samples were taken from the vineyard and from the virgin soil. One fourth of the soil from the vineyard was left untreated and the other three part was treated with nematocide, fungicide or heat.
The results of the field experiment suggest that there was no problem growing grapevine after apple and apple after grapevine, but both species had been inhibited growing after itself. The fungicide and nematocide treatments did not succeed in determining the casual factor of the problem. Heat treatment of replant soil (in pot test) was useful in AS and VNS soils.
Results of biological soil test suggest, that grapevine replant problem do not occur in every vineyard. In fifty percent of soils, no significant differences between the treatments for shoot length, weight of cane, length, diameter and wood:ratio of the fourth internode were observed. In one case, difference was not found in any of the measured characters. However, fruiting bodies of Roesleria pallida (Pers.)Sacc. and the mycelium of Rosellinia necatrix Prill. were observed in this sample. In other samples, there was no significant difference between the treatments, but nematode and fungus infection appeared to be involved in increased shoot growth in nematocide and fungicide treated plants (mycelium of Rosellinia necatrix was detected). In other samples, the fungus infection caused significant difference between the virgin, untreated and fungicide treated soils and infection of Rosellinia necatrix was observed.
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Industrial-scale plantlet production by seed priming and nursery tray seeding method in Sida hermaphrodita L. Rushby
71-74.Views:341Virginia mallow (Sida hermephrodita Rushby), Malvaceae family, is a perspective perennial herb able to yield a biomass crop through the last two decades. In our previous studies, we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. Following these recognition we modifi ed our technique, in such a way that we fractionated the seeds based on their fresh weight / or relative density before we carried out the treatment. When we fi ltered the fl oating seeds on the surface of water, the hot water treatment was performed considerably better on the sink seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination). From all nurseries tray methods preliminary results, it is clear that, Sida seeds, which were treated with these methods, gave the best growing rate for industrial uses.
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Macronutrient accumulation in green pepper (Capsicum annuum L.) as affected by different production technologies
13-19.Views:483Based on the experiments, an assessment was made to determine for green pepper the amount of nutrients extracted by unit weight of fruit and plant parts not meant to be consumed (foliage, stem, root), i.e. the specific nutrient requirements of pepper. A further objective was to find out to what extent nutrient accumulation in individual plant organs was influenced by differences in production technology and soil conditions.
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Agronomic evaluation of different lettuce (Lactuca sativa L.) varieties under unheated plastic tunnel
50-56.Views:497Among the leafy vegetables, lettuce (Lactuca sativa L.) is the most widely grown species in the world. Wide ranges of physical properties of the products are used to assess the degree of maturity. In this study, different lettuce varieties were grown under an unheated plastic tunnel in springtime. We evaluated the agronomic properties of different lettuce varieties, evaluated the condition of the plants, and determined the bioactive substances. Larger head weight can be achieved by the ‘King of May’ butterhead (259.31 g) and ‘Great Lakes 659’crisphead (220.40 g) genotypes. A very strong correlation (r = 0.995) was observed between the lettuce head index, and leaf index and both had a strong positive correlation (r = 0.828 and 0.760) with NDVI. The highest SPAD values were measured for cos lettuce ‘Romaine lettuce’ (44.01) and iceberg lettuce ‘Great Lakes 659’ (42.71). However, these genotypes showed the highest (9.52%; 8.74%) dry matter content, too. The red leaf variety 'Lollo Rosso' had the highest total polyphenol content (181.53 GAE/100 g FW). Among the evaluated properties, iceberg lettuce showed favorable morphology, plant condition, and good dry matter content. In addition, between the loose-leaf lettuces, the red leaf lollo type was outstanding with bioactive content.
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Ecological drought resistance and adaptability of apple varieties
113-122.Views:997For adequate yields in apple plantations, during the long growing period of the fruit primordia, one of the decisive factors is water supply. Indicators of stress are valuable signs for the diagnosis of drought and necessity of watering, i.e. planning the irrigation of plantations. The aim of the present study was to find reliable signs of water stress on apple trees and at the same time conclude on the drought tolerance of different varieties. The plantation of apple varieties grafted on various stocks and cultivated according to different systems (irrigated, non irrigated, integrate and biological) has been examined continuously by leaf analysis. Along the period of growing fruits, measurements were made in the field, then the leaf samples were analysed in the laboratory for composition of pigments, carbohydrates and antioxidants, as well as the histology of the tissues checked. Without irrigation, the mean leaf mass and the relative chlorophyll content (SPAD) of the variety Idared on M4 stocks increases beyond the values of 50 SPAD, whereas on M26 stock and integrated system, it declines. In the collection of varieties, grown according to the „integrated” system, during the development of fruits, ‘Gála’ and ‘Remo’ varieties have been affiliated to the “less susceptible” group regarding drought tolerance because of the leaf morphology, structure and content of SPAD and antioxidants. However, ‘Idared’ and ‘Jonagold’ belong to the “susceptible” group. To the same group are ranged the ‘Akane, Red Rome vanWell, Pink Lady’ varieties. Those varieties reacted to a short period of drought by increased production (content) of carbohydrates. Regarding changes of carbohydrate content ‘Greensleeves’ and ‘Ozark Gold’ varieties belong to the moderately water dependent (requiring) group. In the field, SPAD-tests facilitated the measurement of nutrient-uptake and incorporation, which is proved by the tight correlation between the data of SPAD and the increment of leaf weight (r=0.76–r=0.88), however, this depends on the variety too. SPAD is an indicator of water supply and is related with the density of stomata, cannot used for the selection of water-exigent varieties but for (drought) tolerant ones. In integrated culture, the (drought) susceptible varieties display (water-soluble) hydrophilic and lipophilic antioxidants (ACWand ACL resp.) the quantity of which may have some role in drought tolerance.
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Experimental results of the effects of Hungarian climatic conditions to German disease-resistant industrial apple varieties
53-56.Views:180In the recent years, several disease-resistant apple varieties appeared through the modern breeding technologies. These varieties can be grown with low usage of pesticides, which mean not only environment friendly fruit growing, but the production costs are also lower. In Eastern-Hungary — it is one of the main apple growing regions — a new apple growing structure started to form by the investment of the German Wink Ltd. — several resistant apple varieties were brought from Germany. 'Resistant' refers genetic resistance that usually transferred from the genome of wild apple species. But the fruit of these apple species is not only resistant to diseases, but its quality is poorer, too. In Germany the Re-apples are grown only for the processing industry. Due to climatic circumstances in Eastern Hungary, the first experiences showed better parameters during laboratory measurement, the fruits have more beautiful view, shape and inner characters than usual industrial apples.
In our paper we discuss the results of sensory (consumer) tests, carried out in Eastern Hungary and in the Budapest-region the data analysis of systematic storing experiments (refraction, flesh firmness, weight loss, etc.) and profile analysis of fresh and stored Re-apples. (In the profile analysis the ProfiSens software [4,5] has been used.)
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Above-ground dendromass of sprouted black locust energy plantations: a case study
19-21.Views:386Black locust (Robinia pseudoacacia L.) can be considered as one of the most suitable tree species for establishing energy plantations due to its favourable growing characteristics such as vigorous growing potential in the juvenile phase, excellent coppicing ability, a relatively high resistance to pests. Based on national and international test results the mean annual increment of oven-dry weight of energy plantations regenerated by coppicing generally exceeds the first cycle plantations established by seedlings.
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Studies on the drought responses of apple trees (Malus domestica Borkh.) grafted on different rootstocks
29-36.Views:1135The morphological and chemical changes in the leaves of eight apple varieties grafted on M4, M26 and MM 106 rootstocks were examined during fruit development in non-irrigated plantation. The aim of the experiment was to examine the defensive mechanisms in apple trees under the mild and serious dryness occurring during fruit development. The content of a relative chlorophyll (SPA D value) of the apple leaves on eastern side of the trees was lower than western and southern sides under drought occurring du ring fruit devclop111ent Under sustained drought from first of July to August the SPAD value, the weight of leaves were larger and the stomata density was significantly larger than in the previous periods. There was a close positive significant correlations bet ween SPAD and stoma density both in the group with less sensitive to drought (r=0,8429) and in the large water demanded group (r=0,9209) equally. The rate of increase in SPAD values and the stoma density was slower in the group with drought tolerant, than in the group required good water supply. The varieties being sensitive to water deficite such as Akane. Pink Lady and Red Rome van Well have a l ready responded intensively to short time of drought by the increasing of carbohydrate and antioxidants product ions. At 42 days of sustained drought as soon as the level of carbohydrate in the leaves decreased, the antioxidant s contents of the leaves rised suddenly in the apple varieties with moderate water requirement s as Greensleeves and ldared. Gala apple variety seemed to be able to defend the drought for longer time because there was no change in relatively high level of carbohydrates and antioxidants of their leaves under severe drought during fruit development.
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Effect of 1-MCP (1-methylcyclopropene) on the vase life of Chrysanthemum and Carnation cut flowers
29-32.Views:327The effect of 1-MCP on extending the vase life of chrysanthemum and carnation cut flowers was studied. The flowering stems of both flowers were terminated to 50 cm. in height. Then, the flowers were pre-treated with 1-MCP at 0.3, 0.5 and 0.7g/m3 for 3 hours or 6 hours. The control flowers were placed in ambient air during the treatment. After the period of treatments the flowers were aerated then put in glass vials contained tap water. The vase life determination was conducted in a vase life evaluation room at 22 ± 1°C. Fresh weight determinations of the flowers were made just before the immersion of the flowers into the glasses of water and were repeated on the day when the vase life of the control flowers was terminated. The treatment of 1-MCP at 0.5g/m3 for 6 hours was the most effective treatment of chrysanthemum and carnation cut flowers.
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Effect of Foliar Nutrition on Post-Harvest of Onion Seed under Sandy Soil and Saline Irrigation Water Conditions
85-92Views:356Foliar application has been determined to be an effective nutrients delivery strategy in vegetable and fruits. The enhancement of vegetable and fruit yields affected by foliar nutrients application has been recognized in previously conducted studies with perennial tree crops. The efficiency of foliar nutrition is dependent on soil, climate, fertilizer and the amount of nitrogen used. There is no sufficient information concerning cooperation of foliar nutrition with all nutrients form as well as the rates of these nutrients fertilization in vegetable
and fruit crops. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on inflorescences diameter, flower stalk length, number of seed stem /plant, weight of 1000 seed, germination percentage, seed yield, moisture content, catalase , peroxidase activity and malondialdehyde content of onion seeds. The plants
sprayed with union Zn, union Mn, union feer, shams k, elga 600, boron, and amino x had the highest vegetative growth parameter, germination percent and enzyme activity. The plants sprayed with union Zn, union feer, shams K, magnesium, caboron, hummer and amino X had the highest seed yield ha-1. The seeds were stored for one year to study the effect of different commercial compounds and storage temperatures on germination, moisture content and change in antioxidant enzymes activities of onion seeds during the storage period. Storage at cold temperature showed higher germination percent, moisture content and lower malondialdehyde content than storage at room temperature. The treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer and amino x had the highest germination percent. -
The effect of nitrogen supply on specific yield and fruit quality of apple (Malus domestica Borkh.)
7-21.Views:649The aim of the present study was to determine the effect of nitrogen supply on yield and fruit quality of apple cultivars and to explore the relationships between canopy density and the different fruit quality parameters.
The experiments were carried out at Kálmánháza, in Eastern Hungary in a private orchard in 2003-2004. The response of four apple cultivars ('Elstar', 'Gala Prince', 'Granny Smith', 'Idared') to different nitrogen dosages was studied. The assessed and calculated indices were: yield, fruit diameter, fruit height, shape index, fruit mass, firmness, dry matter content and colouration. The results indicated that nitrogen fertilization has a significant effect on the yield and fruit quality of apple cultivars. The calculated specific yield values were reduced by the application of nitrogen via the increase in the volume of the canopy. An opposit trend was observed for fruit diameter, fruit height and fruit weight, which increased with increasing nitrogen supply. The reduced shape index caused flattening of fruits.
However, the improvement of fruit quality via increasing nitrogen dosage is only virtual, since these dosages increase the fruit size, but firmness, dry matter content and colouration are diminished, which decreases the value of the fruits on the market.
The authors also studied the relationships between canopy density responsible for assimilation and light supply of the fruits and the different fruit quality parameters. The closest linear inversely proportional relationship was found in the case of colouration. There was a negative linear relationship between canopy density and firmness or dry matter content. The relationship between canopy density and fruit mass could be described by a quadratic polinomial function.
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Extending the vase life of Solidago canadensis cut flowers by using different chemical treatments
83-86.Views:292In order to increase the vase life as well as quality of leaves of goldenrod (Solidago canadesis), the effect of 8-hydroxyquinoline sulphate (8-HQS), silver thiosulphate (STS) and l-methylcyclopropene (l-MCP) were investigated. 8-HQS was used as a continuous treatment at 400 ppm with or without sucrose at 50 g/l. The treatment of STS was used by putting the flower bases at 0.4 mM for 6h with or without sucrose at 50 g/l. l -MCP was used at 0.5 g/m3 for 6h dry or in water. Except the treatment of l -MCP in water, the chemical treatments, which were used, led to the increase vase of life of leaves as well as to the inflorescence of cut solidago spikes compared to the control. The best treatment in this concern was 8-HQS at 400 ppm without sucrose, which resulted in longest vase life of leaves as well as inflorescences and lowest percent loss of fresh weight of initial.
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Possibility of stevia (Stevia rebaudiana B.) production in Hungary
29-32.Views:412Artificial sweeteners have harmful effect on human health, so it is great interested in stevia extract. Our experiment was aimed to show the possibility of inland production of stevia. Different plastic mulches were used (black and white) on raised bed and were compared to uncovered (control) plots for yield and state of health of plants. Furthermore we evaluated the depth of cuttings (low cutting until the 6th double leaf; normal cutting until the upper ⅓ of the plant) on the yield depending on the covering method. The plants were transplanted on 9 of May, 2014 on raised bed, 3 rows on it, with 33x25 cm spacing. According to our results, the black plastic mulch produced the highest yield, which can be explained by suppressing effect on weeds, furthermore it kept the soil warm, moist and protected the lower leaves from soil wetness. But, the white sheet mulch could not eliminate weeds around the plants. The total biomass on the black plastic sheet covered plots was the highest, nearly 1000 g pro plant by low cutting. On the control plots the fungi infection reached about 25-30%, which caused leaf falling of plants, decreasing of yield by the end of vegetation period. To summarise, stevia production is possible in Hungary, but it is important to pay attention to the balanced soil moisture and low humidity in the leaf area. It is suggested to cover the soil with plastic sheet or organic materials, such as bark and chippings.
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Appreciation of ethrel on ripening dynamic and on the content of ingredients in processing tomato (Lycopersicon lycopersicum (L.) Karsten) varieties
33-35.Views:292Tomato (Lycopersicon lycopersicum (L) Karsten) is an important crop cultivated in Hungary. Ethrel has been used to advance maturity and promote uniform ripening of processing tomato since 25-30 years in Hungary. The aims of the present study were 1) to evaluate the effects of two different ethrel concentrations on ripening rate, 2) to investigate lycopene content of different maturity stages, 3) to test the effect of ethrel on lycopene content. It is important to note that the experimental year (in July and August) was very rainy and cool. Ethrel was applied at two rates: 1500 and 3000 ppm. The results clearly indicate that Ethrel can be a useful and effective tool of maturity-enhancement, under present circumstances. Ripening concentration increased significantly by Ethrel. In spite of this, Ethrel treatments did not affect lycopene content of examined varieties significantly. The quality of tomato products are characterised by their lycopene content. Colour is highly important quality factor of food products. The range in lycopene contents from all samples evaluated was 48.7 to 113.0 mg kg-1 fresh weight. Also correlations between lycopene content and colour (a*/b*, and chroma) were investigated also.
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Composition and storage of pear cultivars from Nagykanizsa
63-68.Views:740The composition of five pear varieties (‘Abate Fètel’, ‘Bosc’, ‘Williams’, ‘Conference’, ‘Packham’s Triumph’) grown in Nagykanizsa was investigated in three consecutive years (2008, 2009, 2010). A storage experiment was performed in 2008. Four winter pear cultivars were kept in an ULO store for four months and their parameters measured after two and four months. The parameters tested were: size, weight, water soluble solids, titratable acidity, glucose, fructose, sucrose, water soluble pectin, total polyphenols, free radical scavenging capacity, copper and zinc content. The fruits of ‘Conference’ and ‘Bosc’ varieties were found to contain the highest sucrose and total sugar content, while ‘Abate Fétel’ had the lowest sucrose and highest glucose levels among cultivars tested. ‘Williams’ pear was the most acidic. Brix, total sugar, sucrose and water soluble pectin were decreased during storage. Titratable acidity slightly decreased in fruits of Conference pear. Polyphenols and free radical scavenging capacity did not show a significant change during storage.
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Comparative analysis of the production risk of major fruit species
119-122.Views:265Hungary possesses excellent agri-ecological potentials even in an international comparison. Despite their decreasing economic weight, agriculture and food production are of great significance, since the supply of the population with high quality and inexpensive food is a fundamental social demand and one of the essentials of the quality of life. The development of vegetable and food production is of great importance both from the aspect of healthy nutrition and comparative advantages (cheap workforce, professional expertise, traditions of production, climate favourable for quality). Within the Hungarian fruit production apple and sour cherry are essential branches in virtue of their significance. It is widely known, that – though to a different extent in the case of certain fruit varieties – satisfaction of the need for appropriate habitat is one of the important conditions of a decent quality and secure production. In my research, I analyse the territorial distribution, production standard and production risk of the Hungarian apple and sour cherry branches. I did not have the opportunity – because of the imperfections of the database – to compare long time series, therefore I examine the random effects affecting production by using the data of 3 years (they have the same content). I ranked and assessed each habitat and the production risks of certain fruit species compared to each other on the basis of indexes of relative deviation.
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Does foliar nutrition influence the pear fruit quality?
25-28.Views:216The influence of the foliar nutrition on the pear fruit quality (Pyrus communis L.) cv. 'Williams' was studied in years 1997 and 1998. We determined the contents of individual sugars (glucose, fructose, sucrose and sorbitol) and organic acids (malic, citric, fumaric and shikimic) by HPLC (High Performance Liquid Chromatography). The sizes of the fruits (diameter, length, weight) were measured as well as the amounts of soluble solids and titrable acids. The experiment comprised two treatments: the foliar nutrition and the control. In the treatment of foliar nutrition the trees were sprayed five times (from May 22 to July 7) with a foliar fertilizer, which contained 15% of P205, 20% of K20, 0.1% of Mn, 0.1% of B and 0.1% of Mo. The foliar nutrition influenced higher quantities of sugars (glucose, sorbitol, soluble solids) and organic acids (malic, citric) but had no effect on the contents of shikimic acid, pH juice and titrable acids. At the treatment of foliar nutrition a trend of decrease in the contents of fructose, sucrose, fumaric acid, boron, and zinc was noticed.
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Effect of pre-harvest spray of calcium nitrate, boric acid and zinc sulphate on yield and quality of Nagpur mandarin (Citrus reticulata Blanco)
23-28.Views:395An investigation was conducted on uniform, healthy, eight year old trees of Nagpur mandarin (citrus reticulata Blanco) at Fruit Research Farm, Department of Fruit Science at College of Horticulture and Forestry, Jhalawar during 16 September, 2014 to 1 March, 2015. Various doses of calcium nitrate (1.0%, 2.0% and 3.0 %), boric acid (0.2 %, 0.4 % and 0.6 %) and zinc sulphate (0.2 %, 0.4 % and 0.6 %) were sprayed before harvesting and compared with untreated ones. The results obtained indicated that the trees sprayed with T27 i.e. (calcium nitrate 3.0 % + boric acid 0.6 % + zinc sulphate 0.6 %) showed maximum increase diameter of fruit, fruit weight, fruit volume, number of fruits per plant, fruit yield per plant, estimated yield per hectare, reducing sugar, non reducing sugar, total sugar, juice per cent, sensory score and reduced peel thickness over control. Further, T24 treatment combination (calcium nitrate 3.0 % + boric acid 0.4 % + zinc sulphate 0.6 %) has also significantly increased number of segments per fruit, TSS, TSS: Acid ratio, ascorbic acid content, and reduced number of seeds per fruit and acidity percent of fruits.
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Enrichment trials with straw substrate to produce Agaricus bisporus (Lge., Sing.)
7-9.Views:196Everywhere in the world button mushroom is cultivated on heat-treated horse manure or on a compost mixture of straw, horse manure and chicken manure. In the ZKI trials continued on straw which seemed to be a simple and cheap substrate. To improve yield the N-content of the straw was increased by mixing it with wheat bran, alfalfa flour and ProMycel. Enriching agents were added to the dry heat-treated straw (100 "C) at 1, 2, 3 weight per cent. All the 3 agents increased yield as related to control. The highest yield was obtained with ProMycel.
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Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids
39-45.Views:303Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated.
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Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
69-77.Views:268The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.
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Eicosapentaenoic acid production by Phaeodactylum tricornutum under different culture condition
133-136.Views:200Phaeodactylum tricornutum UTEX 640 strain of microalgae was screened under different culture conditions for their capacity to produce eicosapentaenoic acid (EPA) the most abundant polyunsaturated fatty acid (PUFA). In our experiments, the effect of sodium chlorid, nitrogen source, phosphate, initial pH, as well as the CO2 content of the medium on production of the eicosapentaenoic acid (EPA) by P. tricornutum were investigated. The EPA content of biomass was enhanced by the low pH of the medium, with increased concentrations of B17 vitamin and nitrate, and also with decreasing concentrations of sodium chlorid. The EPA is most likely associated with polar (membran) lipids and the role of EPA appears to be involved with membran permeability in microalge. The synthesis of phospholipids, enhances the EPA content of the cells, as expected. The maximum EPA yields were observed under optimum culture condition 43 — 48 mg/g of dry cell weight.
