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Interaction impact of drought stress, nutrient-deficient water, and seed-borne pathogen (Alternaria alternata) on germination and vigor of two tomato varietiesination and vigor of two tomato varieties
48-53.Views:316Tomato (Solanum lycopersicum) is considered one of the leading vegetable plants in the world. This study evaluated the germination and vigor capabilities of ‘Marmande’ and ‘Kecskeméti Jubileum’ varieties under different conditions, including drought stress, nutrient-deficient water, and the effect of seed-borne disease caused by Alternaria alternata when prime and non-prime with salicylic acid. The experiment was conducted in the laboratory in 2023 at Agricultural and Food Sciences and Environmental Management Faculty of University of Debrecen. Results indicated that the ‘Kecskeméti Jubileum’ variety exhibited a superior strength to ‘Marmande’ in the seedling’s dry weight, seedling growth rate, and vigor index under nutrient-deficient water. Nevertheless, when applying levels of drought, the ‘Marmande’ variety had a higher viability rate of 62.5% in comparison with another variety, and the germination rate of the two tomato varieties is at 85.5% in 5% concentration but decreased progressively when exposed to a higher drought concentration of 10%. When using 3 ml of salicylic acid during germination stages, the seedling vigor index of ‘Marmande’ shows a greater index at 165 compared to ‘Kecskeméti Jubileum’, just 108 under the infection of Alternaria alternata. The results of the examination of drought stress, and the effect of Alternaria alternata, one cause of seed-borne pathogens, showed that the percentage germination and vigor ability of the ‘Marmande’ variety performed better than ‘Kecskeméti Jubileum’ under the same conditions.
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Preliminary biochemical studies on a model growing of different tobacco plant (Nicotiana tabacum L.) cultivars
83-87.Views:240The aim of our preliminary studies was to characterize the change in the activity of enzymes PPO and PDX, the concentration of total soluble phenolic compounds and soluble protein content of different tobacco cultivars measured in tobacco plants during cultivation. The results suggest that a shorter cultivation period (13-14 weeks) is more favourable for tobacco plants as protein source than for tobacco industrial use (16-17 weeks). We found some correlation between the concentration of total soluble phenolic compounds and PPO activity data. In the increasing and maximum period of total phenol content the PPO activity was high. But later because of decrease of substrate phenol content the activity of PPO also decreased. We found higher soluble protein concentrations in Virginia than in Burley varieties.
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Post-effects of cytokinins and auxin levels of proliferation media on rooting ability of in vitro apple shoots (Malus domestica Borkh.) 'Red Fuji'
26-29.Views:544Rooting ability of in vitro apple shoots of 'Red Fuji' grown on proliferation media with different hormone content were tested at three IBA levels in root induction media. Rooting percentage could be slightly increased with an increase in IBA concentration in proliferation media. The highest IBA concentration (3.0 mg 1-1) in root induction media showed strong inhibitory effect on rooting capacity of in vitro shoots. The highest rooting percentage (95%) could be achieved by shoots grown on proliferation media containing TOP or BA+KIN as cytokinins before rooting.
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Translocation of diquat dibromide
105-107.Views:258The aims of our work were to answer the following questions: 1. Diquat dibromide at different concentrations is a contact or systemic herbicide? 2. if translocation occurs what is the extent and direction of it. 3. by what concentration it is translocated in hydroponics. It has been stated that diquat dibromide at different dilutions (40-5%) had systemic effect in Robinia pseudo-acacia in the fields. Its translocation has been occurred apically first, and later basipetally. The extent of translocation depended on the concentration. In hydroponics diquat dibromide has crytallized on the leaf surface of Galinsoga parvif lora and all concentrations (40-0.078%) proved lethal. Recognition and application of systemic characteristics of diquat dibromide is reported here for the first time. Improvement of application method is in the focus of our future work.
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Path coefficient analysis of environmental factors influencing flight activity of Apis florea F. and seed yield in carrot (Daucus carota L.)
87-92.Views:222Foraging ecology of insect pollinators visiting carrot flowers (Daucus carota L.) was studied in relation to five environmental variables. The dwarf honeybee, Apis florea L., was the most abundant flower visitors and comprised more than 94% of the total flower visiting insects. Commencement of flight activity occurred when a minimum threshold of environmental variables was surpassed while the cessation was governed mainly by decline in values of light intensity and radiation. In between the commencement and cessation, the foraging population correlated significantly and positively with air temperature, light intensity, solar radiation and nectar-sugar concentration and negatively with relative humidity. Path coefficient analysis, however, revealed that direct effect of temperature was high and positive followed by light intensity and solar radiation while the direct effect of relative humidity was high and negative. The direct effect of nectar-sugar concentration was negative and negligible. Evidently, path coefficient analysis gave a more clear picture of effects than did the simple correlation analysis. Apis florea on an average visited 1.14+0.23 and 22.78+2.57 umbels and flowers/min, respectively during different hours of the day. Furthermore, the insect pollinated plots produced significantly more seeds with heavier weights than those isolated from insect visits.
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The effect of different biostimulators on morphological and biochemical parameters of micropropagated Hosta ’Gold Drop’
22-29.Views:430During in vitro multiplication of Hosta ‘Gold Drop’, 20 g l-1 sucrose, 5.5 g l-1 agar and 4 concentrations (0.1-0.8 ml l-1) of Ferbanat L, Kelpak, Pentakeep-V were added to half-strength Murashige and Skoog (MS) basal medium. As compared to the control and other biostimulators, plants with lower peroxidase activity, larger fresh weight, more, longer shoots and roots, larger leaves were developed on medium containing Kelpak. The best concentration was 0.4 ml l-1 for in vitro rooting, shoot formation, plant weight and ex vitro chlorophyll, carotenoid level, peroxidase activity. Pentakeep was the less efficient biostimulator, increasing of its concentration mostly decreased root and shoot values (furthermore, abnormal callus formation was observed, as non-wanted effect), chlorophyll content and sizes (length, width) of leaves, not only during in vitro propagation but also (as after-effect) acclimatization because of the high mortality and weakly developed survivor plants.
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Response of alternate bearing of “Badamy sefid” Pistachio cultivar to foliar application of some macro and micro elements
109-112.Views:347Response of “Badamy sefid” Pistachio trees to spraying of fertilizer was studied over two years (2006–2007). The boron and zinc fertilizer were used just on swelling time of female flower bud in compare to no fertilization (control). Also nutritional solution combined of nitrogen (400 ppm), phosphorus (P2O5) (380 ppm), potasium (K2O) (520 ppm), Fe (5 ppm), Cu (2 ppm), Zn (2 ppm), Mn (2 ppm) in 1000 liter water per hectare sprayed at first week of May, third week of June and July). The concentration of some macro and micro elements in flower bud and in leaves, also productivity (g/shoot sectional area) mean nut weight, fresh weight of nut per cm2 branch cross sectional area, blanks nuts %, non-split nut %, new shoot growth length after cessation of growth and remained flower bud (%) at the next spring were recorded for the different treatments. The results showed that boron and zinc concentration increased in the bud of sprayed trees in compare to control. Application of nutritional solution decreased the flower bud abscission, also resulted in increased vegetative growth, nut weight, and productivity of trees. In conclusion, spraying of fertilizers in the suitable time to the pistachio trees might be a useful method in decreasing flower bud abscission and mitigating the alternate bearing.
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Determination of biogenic amine content of Tokaj Wine Specialities
87-90.Views:320The knowledge of the finest composition of the Tokaj Wine Specialities is an indispensable task. The presence of the biogenic amines as an eventual combination of hygiene, technology and activity of Botrytis cinerea is a very important point of view in the future. The objective of this study was to measure the biogenic amine-content of Tokaj Wine Specialities.
According to our findings, the histamine-concentration remained lower than the prescribed end values, but ill the case of serotonine, there were significant measured values. During the fermentation period, in the first cycle there was a decrease in the concentration of biogenic amines, but with the advancement of fermentation, an increase was found.
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Cytokinins affect the stomatal conductance and CO2 exchange of in vitro apple leaves
25-28.Views:487Effects of different cytokinin supplies including two types of aromatic cytokinins, such as benzyl-adenine, and 3-hydroxybenzyladenine applied at two different concentrations (2.0 and 6.0 μM) were studied on water and gas exchange parameters in in vitro apple leaves of ‘Royal Gala’ and ‘Freedom’ scions after 3 weeks of culture. Cytokinin supply affected the stomatal conductance of water vapour, transpiration rate and the sub-stomatal CO2 concentration of leaves. Effect of cytokinin depended on its applied type and concentration, moreover on the apple scion. According to the results, the rate of CO2 exchange itself is not usable for characterization of function of photosynthetic apparatus of in vitro leaves. However, measurements of stomatal conductance of water vapour and transpiration rate seemed to be good indicators for stomatal behaviour of in vitro apple leaves.
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The effect of Regalis® (prohexadione calcium) on the reduction of fire blight (Erwinia amylovora) severity in apple trees
61-66.Views:366Due to the lack of effective and non-phytotoxic materials for control of the blossom and shoot blight phase of fire blight in pome fruit trees, two novel control strategies have emerged: shoot growth retardation by bioregulators and applying resistance inducer compounds. Prohexadione calcium (ProCa) is the active ingredient of the bioregulator Regalis® registered in several European countries. The reduction of shoot growth elongation is the most obvious effect of ProCa. Furthermore, it causes significant changes in the spectrum of flavonoids and their phenolic precursors, leading to the considerable reduction of susceptibility to fire blight.
In Poland, potted one-year-old apple trees of cvs. Gala Must grafted on M.26 and Sampion on M.9 (in 2001) as well as Gala Must on P.60 (in 2002) were treated with Regalis® at a range of concentration of 250, 150 or 150 + 100 ppm, respectively. The inoculation of shoots was made with the strain No.691 of E. amylovora (107 cfu/ml), on the 7th and 21st'day after treatments with Regalis. In Hungary, during the years of 2002 and 2003 one-year-old container grown apple trees of the cvs. Idared/M.9 and Freedom/M.9 were treated with the prohexadione-Ca, the active ingredient of Regalis® 100, 150 or 200 ppm, two weeks before inoculation with the Ea 1 strain of E. amylovora (107 cfu/m1).
In Poland, the suppression of fire blight in shoots reached up to 80%, depending on concentration and application time of Regalis®. In Hungary, the effect of prohexadione-Ca treatments, determined by the length of necrotic lesion developed, proved to be better than that of streptomycin used for comparison.
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Efficacy of selected botanical extracts against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) on tomato
31-36.Views:528Tuta absoluta Meyrick, also known as the tomato leaf miner, is a destructive pest that feeds on tomato plants, causing significant economic losses to tomato farmers globally. Traditional methods of controlling Tuta absoluta involve the use of synthetic pesticides, but these methods have drawbacks such as environmental pollution, development of resistance by the pest, and negative effects on non-target organisms. As a result, there is a growing interest in the use of botanical extracts as alternative and safer pest control options. Three bioassay experiments were conducted to test the insecticidal, antifeedant and repellency effects of botanical extracts on Tuta absoluta. The experiments were conducted as completely randomized design (CRD) with three (3) replicates. There were eleven treatments; pyrethrum, neem, warbugia, each at different concentration levels of (8%, 10%, 12%) and controls positive (Indoxacarb 150g/L) and negative (distilled water). In all the bioassay conducted, the effects of all treatments increased over time, except for distilled water control. Synthetic chemical Indoxacarb exhibited significantly stronger insecticidal properties compared to neem, warburgia and pyrethrum extracts. Application of neem at 8%, 10% and 12% and pyrethrum at 12% had higher antifeeding effect on Tuta absoluta compared to the other treatments while warburgia at 8% concentration and indoxacarb had the lowest repellency effect compared to the other plant extracts.
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Investigation of the Relationship between the SO2 Production of Different Yeast Strains and Thiamine Concentration
57-62.Views:302Our general conclusion was that the thiamine amounts of 0,6 and 3,0 mg/1 added prior to wine fermentation, resulted in higher free-S02 level in comparison to the control. Furthermore, among the yeast strains examined in our experiments, Uvaferm BC strain produced the highest free-S02 content under the conditions mentioned before. As regards the dynamics of reductone and SO, production, the concentration of the previous one was practically identical in the middle and at the end of the fermentation while the SO2 content — both free- and total — was lower at the end. Thiamine addition did not cause any difference in the organoleptic properties of wines as proved by sensory analysis.
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Impact of sodium-selenate on the growth of radish (Raphanus sativus L.) seedlings in vitro
113-115.Views:541Selenium (Se) is an essential trace element for animals, microorganisms and some other Eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although it is well known that some species among higher plants are able to accumulate selenium in their tissues, but others are not able to do so, and there is evidence that selenium is needed for the growth of algae, meanwhile the question of essentiality of Se in vascular plants is unresolved. We aimed to study the in vitro growing and to characterise some physiological properties in radish (Raphanus sativus L.) seedlings treated with 0 to 200 mg/1 sodium-selenate. The results showed that lower (2 mg/1) concentration sodium-selenate increased the biomass as well as the total antioxidant capacity of seedlings. The seedling's selenium content showed linear correlation with the sodium-selenate content of the medium.
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Dynamics of the uptake of nutrient elements from the medium of in vitro cultured apple rootstocks
50-53.Views:307Cation uptake of J-TE-F apple rootstocks propagated in vitro was studied by the analysis of culture medium. Test-plants were grown on liquid medium under different light regimes. Samples for tests were taken twice a week.
Media without plants served as controls. The analysis of those showed, that only the uptakeable iron-content changed depending upon light treatment. The concentration of all other cations was considered unaltered.
As a result of analysis, it could be established, that elements present in the media were taken up in different rates by plantlets: Cu, P and Zn were utilized totally, but only 50% of K and 20 to 40% of Ca and Mg were taken up under the light treatments applied.
The dynamics of the uptake process was also observed. It was registered that they differed in the case of some cations. So Ba was utilized at the beginning of subculture, others for example B in the later phases. Some elements disappeared unevenly so K, P but the whole quantity is taken up during subculture.
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First evaluation of fruit quality of some new and old sour cherry cultivars in Hungarian climatic condition
37-43.Views:14342Indeed, in the last decade a large number of new sour cherry cultivars with new notable flavor characteristics have been released. There is no enough information related to qualitative characteristics which consistently segregated based on their predominant sensory characteristics. The present study carried out at tow consecutive years (2008 and 2009) and examined some qualitative characters which are the main importance in the specialty cherries trade, The main objective was to determine fruits quality of Érdi bőtermő, Oblacsinszka, Debreceni bőtermő, Csengődi, Pándy 279, Éva, Kántorjánosi, Újfehértói fürtös and Petri sour cherry cultivars. Changes observed in major characteristics such as fruit detachment force, width, length, weight, peduncle length, flesh firmness, brix, citric acid, Suger/acid ratio and pit weigh.While observed no change in flesh/pit ratio and Coordinate of colour (X*, y*, x*), hue angle and chroma value, in different years. In all measured factors, there was significant difference between selected cultivars of sour cherry fruit. Debreceni bőtermő showed higher values of fruit detachment force in 2008, while had the lover of fruit detachment force in 2009. Fruit firmness of “Érdi bőtermő” were monitored at lover value in both 2-year.
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Comparative analysis of Hungarian Matricaria recutita (L.) Rausch. populations
81-85.Views:412Matricaria recutita L. is a traditional medicinal plant in Hungary and its drug is known as „Hungaricum", world-wide. Plant samples and seeds were collected from 12 different habitats of three significant geographical regions of Hungary in 2001. Morphological, production biological and chemical properties of samples were examined. In relation to the morphological characteristics, a negative correlation (r= -0.75) could be observed between the average height of the plants (height of flowering shoots) and the pH value of the soil. According to the composition of the essential oil, the populations accumulating typically chamasulene (10-20%), a-bisabolol (30-50%) or bisabolol-oxid (30-50%) could be completely distinguished. Concerning the flavonoid composition the quantity of apigenin-7-glucoside was outstanding in the populations originating from the Great Hungarian Plain, it has reached the concentration of 1.8-2.8 mg/g, while the samples collected in Transdanubia could be characterised by much lower level of apigenin-7-glucoside (around 1.5 mg/g).
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Role of wholesale markets in fresh fruit and vegetable distribution in Hungary
46-50.Views:239The primer function of fruit and vegetable wholesale markets — which was the physical concentration of supply and demand previously — is changing gradually as consumer habits change and logistics functions (e.g. product manipulation, redistribution without the physical presence of the product) become more important with the technical development. Mainly individual growers sell their product to retailers at the Hungarian regional producers' markets and the Budapest Wholesale market on NagykOrosi Street, which is the largest wholesale market in Hungary regarding area, produce arrival and turnover. Parallel to the establishment of Producers' Organisations (P0) their market presence should be urged in selling their produce and also in ownership — and decision-making procedure — of the markets. The produce auction system and the on-line sales experiences of New Jersey State (USA) can contribute to the investigation of development possibilities and realization of development of Hungarian wholesale markets.
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Development of in vitro propagation system for Atriplex halimus L.
123-129.Views:381Explants excised from adult shrubs were surface sterilized and cultured on Murashige and Skoog (MS) basal medium in the presence of plant growth regulators (PGRs) at different concentrations. A high multiplication rate of 7.2-fold was achieved every four weeks on MS medium supplemented with 4.44 μM BA, 0.49 μM IBA and 0.58 μM GA3. Rooting was achieved with 73% efficiency within 2-4 weeks on agar-gelled MS basal medium free of PGRs. Rooted plantlets were gradually acclimatized to field conditions over 5-6 weeks with 65% efficiency. For in vitro selection for salt tolerance, MS medium was supplemented with increasing concentrations of NaCl ranging between 25 and 1000 mM. This study has demonstrated that in vitro shoots could tolerate up to 600 mM NaCl with optimal growth at 200 mM, while higher concentrations of NaCl affected growth negatively. Growth and shoot number decreased with increasing NaCl concentration with all plantlets died at 1000 mM NaCl.
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Allelopathic effect of the Cladonia verticillaris lichen extracts and fumarprotocetraric acid on the early growth of germinating seeds and seedlings in Allium cepa L.
31-35.Views:280The allelopathic activity of the different extracts of the lichen Cladonia verticillaris and fumarprotocetraric acid on the early growth of A cepa (IPA 6) seedlings depends on their chemical composition and concentration, respectively. It was observed that the length of the radicle was significantly stimulated by fumarprotocetraric acid at high concentrations and by the total extract of C. verticillaris thalli, which contained a high level of fumarprotocetraric, acid confirmed by HPLC-technique. In addition, it was found, that the phosphate buffer extract, which contained high level of methyl (3-orcinol carboxilate measured by HPLC, reduced the length of the hypocotyls significantly. Under our experimental conditions there was no influence of the different types of extract and fumarprotocetraric acid on the ratio of seed germination, in relation to the control. From the study of HPLC it was found that fumarprotocetraric acid and methyl 13-orcinol carboxilate were present in all extracts at different concentrations, depending on the method of extraction.
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Analysis of the polyphenolic composition of red wines with particular respect to the resveratrol concentration
17-22.Views:353In our work we have studied red wines of some vine-districts. In the centre of interest was resveratrol. We declared, there was not significant difference between varietys in polyphenol comparison. There was significant variety in anthocyanin and colour-intensity.
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Nutrient demand of stone fruits
19-23.Views:258Effects of nitrogen, phosphorus and potassium fertilization were investigated on the change of nutrient content, vegetative and generative production of apricot, peach and sour cherry trees, as well as on frost hardiness in long term experiments. Nitrogen and phosphorus fertilization increased only the concentration of these elements in cherry leaves without effect on growth and yield. Consequent potassium effect was proved on these stone fruit species. Effect on yield appeared following the first higher crop load.
Potassium supply has positive effect on frost hardiness of apricot and sour cherry flowers and peach flower buds.
In peach, the lime content of soil decreased the yield but it could be compensated by potassium dressing to some extent. Favourable nutrient boundary values were determined for soil and foliage.
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Floral biological investigations of apple cultivars in relation to fire blight
9-14.Views:368Floral activity was studied in two apple cultivars: an Erwinia-tolerant (Treedorn') and a sensitive one (`Sampion'). Since more types of protogyny occur in apples, the period of stigma activity is different. Papillae of the exposed stigma in flowers of 'Freedom' function longer (usually more than a week) than in the delayed homogamous `Sampion'. Despite of this, cv. 'Freedom' is tolerant to Erwinia amylovora (Burr.) Winslow et al., suggesting no relationship between the floral biological type (including the exposure and longevity of stigma) and the infection by E. amylovora. According to SEM micrographs, nectary stomata in `Freedom' are already open in the flower bud, where nectar secretion starts and continues until the senescence of the stigma. However, the long period of nectar secretion does not create optimal conditions for bacterial growth, since nectar production is scant in the flowers of 'Freedom'. The surface of the nectary, its nectar-retaining capacity, and the amount and concentration of nectar may influence the susceptibility of apple cultivars. It is manifested well by the smooth nectary surface with nectary stomata rising slightly above the epidermis in flowers of cv. 'Freedom', contrasting the wrinkled, striate nectary surface with slightly sunken stomata in the flowers of 'Sampion'.
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The effect of Ferbanat L nano-fertilizer on the growing of Petunia x grandiflora ’Musica Blue’
107-109.Views:449During our experiment, the effect of Ferbanat L concentrations were examined by Petunia x grandiflora ‘Musica Blue’ production. The leaf and shoot length, number of flower buds, diameter of flowers, and the date of appearing the first buds were measured. The solution of Ferbanat L in 0.1 % concentration was the most effective on shoot length (21.7 cm). Remarkable increase was observed by the other treatment groups as well comparing to the control group (5.6 cm), the plants treated with solution of Ferbanat L 0.2% (16.5 cm) and 0.3% (14.4 cm) had significant effect as well. The nano-fertilizer had not positive effect on the other examined parameters as leaf and flower size, number of flower buds or chlorophyll content. The culture period shortened five days.
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Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.)
57-60.Views:332Dry seeds from two cultivars of common bean (Phaseolus vulgaris L.) were germinated on sterile cotton and sterile deionized distilled water. Cotyledonary node tissue of seedlings were cultured on Murashige and Skoog(MS)-based media supplemented with different combination of N6-benzyl-aminopurine (BAP) and indole-3-acetic acid (IAA), and benzyladenine (BA) and a-naphthaleneacetic acid (NAA). The results revealed that the regeneration percent and the average number of buds and shoots per explant were influenced by the type of explants and exogeneously added hormones. Multiple shoot induction on dry bean cotyledonary node that contain 4-5 mm from cotyledons and hypocotyl on a medium containing full concentration of MS inorganic salts supplemented with 0.5mg/1 BA and 0.1mg/1 NAA was feasible and the method can be applied in transformation experiments.
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Analysis of amino nitrogen content affecting fermentation and wine quality
13-16.Views:342A suitable amount of nitrogen source is needed for the optimal process of alcoholic fermentation. The professional literature mentions the complexity of nitrogen compounds exploitable for yeast-plants as an assimilable or immediately assumable nitrogen source. Former experiments proved that in case of low nitrogen concentration , yeast-plants produced a bigger quantity of hydrogen sulphide. According to the findings above, it is not indifferent when and how we complement the nitrogen content of the must. The aim of our present work was to elaborate such a quick, easily applicable method that can be used for routinish measurements in the viticultural practice.
