Vol 12 No 4 (2006)
Cikkek

Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA

Published September 26, 2006
A. Szőke
Department of Genetics and Plant Breeding, St. István University, Gödöllő H-2103, Hungary
E. Kiss
Department of Genetics and Plant Breeding, St. István University, Gödöllő H-2103, Hungary; HAS-SZIU Molecular Plant Breeding Research Group, Gödöllő H-2103, Hungary
O. Toldi
Agricultural Biotechnology Center, Gödöllő H-2103, Hungary
L. Heszky
Department of Genetics and Plant Breeding, St. István University, Gödöllő H-2103, Hungary; HAS-SZIU Molecular Plant Breeding Research Group, Gödöllő H-2103, Hungary
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How to Cite

APA

Szőke, A., Kiss, E., Toldi, O., & Heszky, L. (2006). Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA. International Journal of Horticultural Science, 12(4), 75-79. https://doi.org/10.31421/IJHS/12/4/683

Abstract

Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.

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