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Virus susceptibility and resistance of Hungarian pepper varieties
68-73.Views:305The aim of our study was to examine susceptibility or resistance of 18 pepper varieties to four viruses [tobacco mosaic
tobamovirus (TMV), sowbane mosaic sobemovirus (SoMV), NTN strain of potato Y potyvirus (PVYNTN) and cucumber mosaic cucumovirus (CMV). Out of the 18 varieties, 13 were resistant to CMV infection. Thirteen varieties were susceptible to TMV, while five ones (Dabora Fl, Brill Fl, Feherozon Synthetic, Ciklon Fl, Cecil Fl) showed only local hypersensitive reaction. All of the tested pepper varieties showed resistance to SoMV. Eight varieties (Tuba, Fehérözön Synthetic, Boni, Alba Regia, Korona, Édesalma, Cecil Fl, Star) were found to be resistant to PVYNTN. Out of the examined varieties five (Boni, Alba Regia, Korona, Édesalma, Star) were resistant to three viruses (SoMV, CMV and PVYNTN). Only one (Cecil F1) displayed complex, extreme resistance to SoMV, PVYNTN, CMV and hypersensitive reaction to TMV, therefore this hybrid is very important in pepper breeding and growing for virus resistance.
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Effect of three organic fertilizers treatments on sensory evaluations of baby spinach (Spinacia oleracea L.)
59-63.Views:540Sources of fertilizer are one of the major factors influencing baby spinach leaf texture, sweetness, bitterness and after-taste. However, the effects of fertilizer sources on baby spinach growth performance and consumer preferences are not known. A survey was carried out at the Seke Teachers’ College (SKC) community, Zimbabwe to determine the consumer preferences on the baby spinach grown on the organic fertilizer (cattle, poultry and goat manures) as well as on control inorganic fertilizers (7% N, 14% P, 7% K). The study used 32 females and 30 males as panellists for sensory evaluations. Organoleptic tests were performed for the baby spinach leaves using a panel of 62 testers. An interval line scale (16 cm long) was used to measure the liking for sweetness, colour, bitterness and after-taste. A 9-point hedonic scale was used to decide the overall preferences. Organoleptic tests showed significant differences (P<0.05) in appearance and taste between the inorganic and organic fertilizers used. The baby spinach leaves grown on organic fertilizers was preferred more compared to that grown on inorganic fertilizer (control). Therefore, it is recommended to use organic fertilizers in baby spinach production in order to satisfy consumer preferences.
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Effect of water supply on nutrient transport in grapevine varieties
64-68.Views:264The effect of water supply on availability of macro nutrient elements (N, P, K) by the plants in the soil and their transport in the plants were examined. In a field experiment two grapevine varieties characterized by higher (White Riesling B 7) and lower (Kövidinka K 8) water requirement were compared on the basis of N, P, K concentrations of leaf blades, petioles and berries. A different water supply of the vineyard was achived by striped coverage of the soil with plastic foil to exclude rainfalls from the beginning of May to ripening. Humidity of the soil decreased as the vegetative phase advanced. Soil cover resulted in 25-30% decrease of the water content in the soil at flowering but this difference gradually disappeared till veraison (i.e. the start of intense growth of the berries). The water consumption in the White Riesling B 7 plantation was more intensive. Mobility and availability of N, P, K in the soil was restricted by water exclusion (i.e. plastic soil cover) at flowering. Nitrogen was slightly affected, whereas P and K were in a higher extent. Comparing the transport of nutrient elements in the two varieties, leaf blades of Kövidinka K 8 contained less N and more P and K than White Riesling B 7 at flowering and more N and P and less K at ripening. Water deficiency inhibited K accumulation in the berries of White Riesling B 7, while this effect did not appear in Kövidinka K 8.Water exclusion decreased the yield of White Riesling B 7 already at lower bud loading, the yield of Kövidinka K 8 was affected only at higher bud loading. The higher yields of the treatments in Kövidinka K 8 plantation support the superior performance of this variety under the hot and dry climate of the Hungarian Great Plain.
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Reaction of different Capsicum genotypes to four viruses
61-64.Views:318The objective of this study was to examine the reaction of 44 Capsicum genotypes to common strain of Tobacco mosaic virus (TMV-C/U1), Obuda pepper virus (ObPV), NTN strain of Potato virus Y (PVYNTN) and legume strain of Cucumber mosaic virus (CMVU/246). Reaction (extreme resistance, hypersensitive reaction, latent susceptibility, susceptibility) of the tested Capsicum species/hybrids and breeding lines seemed to be greatly depending on hosts and viruses. Out of the breeding materials 4/99 F2 and IX-8 in to CMV-U/246, while 32.Bogyisz. type, VI-57 ii. 57/83 and V-12=19/98 to TMV-C/Ui showed extreme resistance. Two lines (V-25 F1=32/98 F1 and V-27 in F4=35/98 F4) showed hypersensitive reaction to ObPV. Latent susceptibility to PVYNTN was observed in case of all eleven tested Capsicum genotypes and in case of several lines to TMV-C/U1, ObPV and CMV-U/246. Other breeding materials proved susceptible to the mentioned viruses. Pepper genotypes showing extreme resistance and hypersensitivity could be used for resistance breeding to viruses.
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Fruit injury in organic fruit production and its relationship to brown rot caused by Monilinia spp.
7-9.Views:631In a two-year Hungarian study, the temporal progress of brown rot incidence and various injury types were studied in organic fruit orchards and the relationship between brown rot and injury types was determined. Results showed that brown rot reached an almost 20% incidence level in both years. Total injury incidence reached up to 5.3 and 19.8% in the two years. In all cases, insect injury incidence was the highest among injury types in most assessment dates. Incidence levels of other injury types (bird injury, mechanical injury and other injury) began to increase, but none of those reached levels >4%. Pearson’s correlation coefficients showed that brown rot incidence correlated significantly with the incidence of insect injury. In addition, brown rot incidence and the incidence levels of bird injuries was also significantly correlated. High injury and brown rot incidence levels suggest further improvements on organic fruit protection.
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The effect of modified bacterial virulence to host-pathogen relationship (Phaseolus vulgaris L. Pseudomonas savastanoi pv. phaseolicola)
53-56.Views:550The Pseudomonas savastanoi pv. phaseolicola is one of the most expressive biogen stressors of the bean (Phaseolus vulgaris L.) in Hungary. The chemical and agrotechnological defence is inefficient, so breeding is the only workable way. The conventional cultivars are susceptible to PS while most of the new industrial varieties have genetic resistance to the pathogen. The genetic background of resistance is, however, a complex system in the bean. Leaf resistance is a monogenic system, but this gene is not expressed in juvenile stage of the host. The pathogen species can be divided into different races. After inoculation with virulent strains, typical symptoms appeared on the leaves. To understand the details of host-pathogen relationships, there were carried out experiments using bacterial strains with altered virulence. Six transposon mutants of the PS were tested. Our main objective was to test these modified bacterial strains on bean cultivars of known genetic background. First we analysed the symptoms, and then the correlation between the symptoms and the multiplication of mutant bacteria. Three cultivars (Cherokee, Inka and Főnix) were tested.
The infection by the virulent PS isolate produced typical symptoms on the three cultivars tested. Mutant bacteria (except strain 756) did not cause any significant symptoms on the hosts. The mutant 756 induced visible symptoms on the cultivars Cherokee and Inka. On Cherokee there were small watersoaked lesions, and HR (hypersensitivity reaction) was detected on Inka, but this was restricted to some cells only (mikro HR). The rate of multiplication of the wild type strain was much higher than the multiplication of the mutants. Bacteria were detected in the cotyledons and primordial leaf, but there is not any substantial number of bacteria in leaves, except for strains 757, 1212 and 1213. The rate of multiplication of strain 756 was intermediate. These, and other experiments can help to understand the genetic background of resistance and the host-pathogen relationship in the Pseudomonas-bean pathosystem.
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Incidence of two leaf fungal diseases in two plum training systems
15-17.Views:1158In a two-year study, we aimed to determine the susceptibility of four plum cultivars to two fungal pathogens of plum (Stigmina carpophila and Polystigma rubrum) in two training systems with tree spacings of 4 x 1.5m and 6 x 3m. Results showed that shothole symptoms were not detected on cvs ’Bluefre’ and ’Stanley’ in August, 2016. Disease incidence was above 50% in the case of ’Čačanska lepotica’ in both training systems in 2016. There were no significant difference between the two training systems. Shot hole incidence was lower in the 6 x 3m spacings compared to the 4 x 1.5m spacings on cv ’President’ in 2016. Cultivar ’Čačanska lepotica’ showed the highest incidence of Stigmina carpophila in the 4 x 1.5m spacing in 2017. Disease incidence of Stigmina carpophila was significantly lower in the 6 x 3m spacing compared to the 4 x 1.5m spacing. Shothole incidences on cv ’President’ were similar to the values in 2016 ranging from 40% to 60%. Leaf disease incidence was higher in the 4 x 1.5m spacing compared to the 6 x 3m plot. Low disease incidence (below 10%) was observed on cv ’Stanley’ in 2017 and only in the 4 x 1.5m spacing. There were no visible symptoms of blackhorn dotty in 2016 due to inadequate weather conditions for the Polystigma rubrum fungus. However, all the four cultivars were infected by Polystigma rubrum in 2017. The most susceptible cultivar was cv ’Čačanska lepotica’ with the highest disease incidence in the 4 x 1.5m spacing. Disease incidence of this cultivar was lower in the 6 x 3m spacing which was significantly less than in the 4 x 1.5m spacing. The least susceptible cultivar was ’Bluefre’ and symptoms were observed only in the spacing of 4 x 1.5m. The disease incidence of cv ’President’ was similarly low to cv ’Stanley’ in both spacings.
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Spore dispersal, diurnal pattern and viability of Monilinia spp. conidia and the relationship with weather components in an organic apple orchard
17-19.Views:628In a two-year Hungarian study, spore dispersal diurnal periodicity and viability of Monilinia spp. and their relation to weather components were determined in an organic apple orchard. Conidia of Monilinia spp. were first trapped in late May in both years. Low number of conidia were trapped until end-June. Thereafter, number of conidia continuously increased until harvest. Conidia in a 24-h period showed diurnal periodicity pattern, with th highest concentration in the afternoon hours. Spore viability with FDA staining showed that viability of
conidia ranged from 45 to 70% with showing lower viability in the dry than in the wet days in both years. Temperature and relative humidity correlated positively with mean hourly conidia numbers in both years. Mean hourly rainfall was negatively but poorly correlated with conidiacatches in both years. Results were compared and discussed with previous observations. -
Leaf number and plant height of three Canna x generalis cultivars
22-24.Views:367The aim of this three-year study was to evaluate the effect of three treatments (thiophanate methyl, charcoal dust, and control) on leaf number and plant height on three Canna x generalis cultivars (City of Portland, Richard Wallace and Robert Kemp). Results showed that the 3-year means of leaf number ranged from 13.9 to 23.1 for the three cultivars. The largest leaf numbers were for cultivar Robert Camp (23.1 in 2010) and the lowest for cultivar City of Portland (13.9 in 2011). Thiophanate methyl treatment produced the highest number of leaves and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Numbers of leaves in charcoal dust treatments were larger than the control treatments but it was not significantly different. Results on plant height ranged from 69.2 to 160.5 cm for the three cultivars. The largest plant height was for cultivar Robert Kemp (160.5 cm in 2011) and the lowest for cultivar City of Portland (69.2 cm in 2011). Thiophanate methyl treatment produced the highest plant height and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Plant heights in charcoal dust treatments were larger than the control treatments but it was not significantly different.
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Identification of plant taxons by isoelectric focusing
65-67.Views:456Differences were demonstrated in esterasei coenzyme pattern of some essential oil producing plants belonging to the Apiaceae family — fennel (Foeniculum vulgare Mill.), angelica (Angelica archangelica L.), lovage (Levisticum officinale Koch.), dill (Anethum graveolens L.), coriander (Coriandrum sativum L.), anise (Pimpinella anisum L.), caraway (Carum carvi L.) — as well as differences between two varieties of fennel seed by using isoelectric focusing. That method provides quality control in essential oil plants and is suitable to describe isoenzyme pattern characteristic for taxons.
Based on our findings, isoelectric focusing seems to be suitable for identification and differentiation of different plant samples, providing an easy tool for further processing as well as for breeding.
Our further aim is to apply that method to differentiate among samples belonging to the same species according to their value of inner content.
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Preliminary results on salicylic acid treatment on brown rot caused by Monilinia laxa on Jumbo Cot fruit, Prunus armeniaca L.
37-39.Views:1201The effect of salicylic acid on reducing brown rot caused by Monilinia laxa infection in postharvest apricot fruit Prunus armeniaca L. Freshly harvest Jumpo Cot fruit were treated with water as control treatment and other group treated with salicylic acid 2 Mm as induced resistance treatment, Fruits were infected by M. laxa (1 × 10-3spores ml-1) and incubated at 25 oC for 2, 4 and 6 days. Treatment fruits with salicylic acid resulted in direct effect on mycelial growth as in the salicylic acid treatment the growth reached to 45% after 6 days of incubation while in the control treatment it reached to 100%.
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A complex system for the production of pathogen-free grapevine propagating material
59-62.Views:533The use of pathogen-free planting stock for new vineyard establishment is a key component in the maintenance and expansion of vine and quality table grape production. The success of the necessary changes in the structure of the grape industry is forced by the globalization process, the climate change, the rediscovery of autochton varieties as well as breeding of new tolerant and resistant varieties. The renewal of vineyards largely depend on the availability of planting stocks. Serbia and Hungary found a common interest in establishing pathogen-free stock materials from newly breed resistant varieties and clonal selections of varieties which are traditional in the Serbian-Hungarian border area. During a cross-border cooperation program a complex system for the production of pathogen-free grapevine propagating material was established. Using heat therapy, in vitro shoot tip culture and traditional and molecular diagnostic techniques new pathogen-free stock materials were established from 26 varieties. They have been or will be tested for the presence of most important grapevine viruses, phytoplasmas, as well as bacterial and fungal pathogens. The complex system applying green grafting for indexing on grapevine indicators can shorten the duration of the procedure from 4 years to two-three years.
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Nutritional quality, fruit shape and relationships among exotic and local Capsicum pepper genotypes in Uganda
33-39.Views:529Twenty-one hot pepper genotypes comprising of local (15) and exotic (6) types (C. annuum, C. frutescens and C. chinense) were characterized for selected fruit traits after propagation in a glasshouse at the Makerere University Agricultural Research Institute Kabanyolo in Central Uganda using a completely randomized design with three replicates. Ripe fruits were harvested and analyzed; traits evaluated were all significantly different at P<0.05 with variations in quality attributes. The genotype OHA-B305-10 had the highest ascorbic acid content (128.86 mg/100 g) and is recommended for improvement of both local and exotic genotypes targeting the fresh market. Genotypes CAP0408-12 and UG2 WE0511-22, with highest total soluble solids (16.17 ºBrix) and dry matter content (28.59%), respectively should be used in improvements for industrial use or processing to products such as chilli powder or flakes. BRS-M205-04 with highest titratable acidity (1.04%) can be used in enhancing shelf life of genotypes with low titratable acids as well as for the fresh market. In spite of the intraspecific relationships among genotypes, significant differences were observed in their quantitative traits. These genotypes will, therefore, be useful in improving the quality of hot pepper fruit in Uganda.
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Pre-scaling up evaluation of banana technology under irrigation conditions in Abergelle District, Wag-himra Zone, Ethiopia
39-44.Views:672The study was conducted to compare the improved banana technology against the local production technique to enhance demand-driven banana technology up-scaling and diffusion. Data were collected at the field and farmers' levels. Descriptive and inferential statistics, cost-benefit analysis and matrix ranking were employed for analysis. The result revealed that the average yield (38.40 ton ha-1) of improved banana technology had a significant yield advantage (47.24%) over the local practice (p<0.05). Despite the higher cost of production, its net return was by far higher than the local practice. The benefit-cost ratio also displays that 9.49 Ethiopian Birr (ETB) profit per 1.00 ETB investment in an improved technology package. The overall farmers' perceptions were laid under strongly agree and agree categories, and 95% of them believed that the improved banana technology was appropriate for their area and hence accepted with full confidence. The respective organizations working on rural livelihood improvement are therefore advised to up-scale the improved technology for the wider community based on the irrigation potential.
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The in vitro and in vivo anatomical structure of leaves of Prunus x Davidopersica ‘Piroska' and Sorbus rotundifolia L. ‘Bükk szépe'
92-95.Views:524Immature in vitro leaves showed similar structure of the mesophyll tissue to the immature field-grown (in vivo) leaves of Prunus x davidopersica `Piroska'. Mature leaf anatomical characteristics of in vitro plantlets differ from the field-grown plants. The mesophyll tissue of in vitro plantlets were thinner than the in vivo plants and consisted of only one layer palisade parenchyma, the shape of the cells and the structure of spongy parenchyma basically differed from the field-grown plants. In the case of Sorbus rotundifolia similar anatomical differences were found both in vitro and in vivo as in the case of Prunus x davidopersica `Piroska'.
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Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia
74-83.Views:356Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.
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Single and dual effects of different cytokinins on shoot multiplication of different apple scions
76-78.Views:551Shoot multiplication responses of three apple scions to different concentrations of BA and BAR as single source of cytokinins and in combination with two concentrations of KIN were studied. The effects of hormones depended on genotype, type and interactions of different cytokinins. Use of BAR significantly enhanced the shoot multiplication of cv. Jonagold (6.5 shoots per explant). The multiplication rate of cv. Jonagold could not be improved by using the combination of BAR and KIN. The best proliferation was achieved by 1.0 mg 1-1 BA combined with 1.0 mg 1-1 KIN of cv. Prima..(8.1) and of cv. Galaxy (10.4).The effect of 0.5 mg 1-1 BA along with 1.5 mg 1-1 KIN was similar on multiplication rate (10.9) of cv. Galaxy.
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Genetic engineering of apple (Malus domestica Borkh.) for resistance to fungal diseases using g2ps1 gene from Gerbera hybrida (Asteraceae)
15-12.Views:420In the present study, g2ps1 gene from Gerbera hybrida coding for 2-pyrone synthase which contribute for fungal and insect resistance was used. The aim was to work out an efficient approach of genetic transformation for apple cvs. ‘Golden Delicious’, ‘Royal Gala’ and ‘MM111’, ‘M26’ rootstocks for improving their fungal resistance using genetic engineering techniques. Adventitious shoot formation from leaf pieces of apples studied was achieved using middle leaf segments taken from the youngest leaves from in vitro-grown plants.
Optimum conditions for ‚direct’ shoot organogenesis resulted in high regeneration efficiency of 0%, 95%, 92%, 94% in the studied apples respectively. Putative transgenic shoots could be obtained on MS media with B5 Vitamins, 5.0 mg l-1 BAP, or 2.0 mg l-1 TDZ with 0.2 mg l-1 NAA in the presence of the selection agent “PPT” at 3.0-5.0 mgl-1. Shoot multiplication of transgenic shoots was achieved on: MS + B5 vitamins + 1.0 mg l-1 BAP + 0.3 mg l-1 IBA, 0.2 mg l-1 GA3+1.0 g/l MES+ 30 g/l sucrose + 7.0 g/l Agar, with the selection agent PPT at 5.0 mg l-1 and were subcultured every 4 weeks in order to get sufficient material to confirm transformation of the putative shoots obtained. Six, seven, one and six transgenic clones of the apples studied respectively have been obtained and confirmed by selection on the media containing the selection agent “PPT” and by PCR analysis using the suitable primers in all clones obtained for the presence of the selection” bar gene (447 bp) and the gene-of- interest “g2PS1” (1244 bp), with transformation efficiency of 0.4%, 0.6%, 0.1% and 0.3% respectively. These transgenic clones were multiplied further in vitro in the presence of the selection agent ‘PPT’ and rooted in vitro. Rooted transgenic plantlets were successfully acclimatized and are being kept under-containment conditions according to the biosafety by-law in Syria to evaluate their performance for fungal resistance . -
Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis
Views:438Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.
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Effects of farmyard manure (FYM) and blended NPSB fertilizer rate on growth and yield of potato (Solanum tuberosum L.) in Gewata, Kaffa, southwest Ethiopia
14-22.Views:333Potato (Solanum tuberosum L.) is an essential crop for nutrition and food security and generating income to small-holder farmers in Ethiopia. Productivity of the crop is, however, limited by poor nutrient management practices and low soil fertility. In most parts of potato growing areas of Ethiopia, soil are deficient in macro (nitrogen, phosphorus and sulfur) and micro nutrients (boron) which are known to affect production and productivity of potato. A field trail was accompanied to determine the growing response of potatoes ’Belete varieties’ to combine the treatment of farmyard manure and blended NPSB (nitrogen, phosphorous, sulfur and boron) fertilizer at Gewata, southwest Ethiopia during 2019/2020 main cropping season. The variables are laid out four level of farmyard manure (0, 5, 10 and 15 t/ha) and four level of blended NPSB fertilizer (0, 50, 100, and 150 kg/ha). The treatments were organized in a completely randomized block design with three replications using a 4x4 factorial layout. SAS (version 9.4) was used for data were analyzed. The finding indicate that, combined application of FYM (farmyard manure) with NPSB significantly (p<0.01) affects plant height, average mass of tuber, number of marketable and unmarketable tuber per hill, and tuber yields per plot. It also significantly (p<0.05) influenced days to flowering, maturity, and tuber number per hill. The highest tuber weight (99.33 g), tuber number per hill (9.67), and yield (34.10 kg) per plot were recorded from the mixed fertilization of 15 t FYM with 150 kg/ha NPSB mineral nutrients. As a current finding, it can be stated that the combined use of FYM (15 t/ha) and NPSB blended fertilizer could improve potato growth and yield in the study area (150 kg/ha).
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Review of the molecular background of self-incompatibility in rosaceous fruit trees
7-18.Views:432This review gives a presentation of the gametophytic self-incompatibility system in the roscaeous fruit trees. Studies to discover the pistil (S-ribonucleases) and pollen-part components (F-box molecules) are summarized and models for the self-incompatibility reactions as well as their molecular background are discussed. We describe how mutations within the S-RNase or F-box genes can contribute to the transition from self-incompatibility to the self-compatible phenotype in many fruit tree crops. The current state of the arts is compared to the information obtained in other plant species possessing similar incompatibility system.
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Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.)
57-60.Views:332Dry seeds from two cultivars of common bean (Phaseolus vulgaris L.) were germinated on sterile cotton and sterile deionized distilled water. Cotyledonary node tissue of seedlings were cultured on Murashige and Skoog(MS)-based media supplemented with different combination of N6-benzyl-aminopurine (BAP) and indole-3-acetic acid (IAA), and benzyladenine (BA) and a-naphthaleneacetic acid (NAA). The results revealed that the regeneration percent and the average number of buds and shoots per explant were influenced by the type of explants and exogeneously added hormones. Multiple shoot induction on dry bean cotyledonary node that contain 4-5 mm from cotyledons and hypocotyl on a medium containing full concentration of MS inorganic salts supplemented with 0.5mg/1 BA and 0.1mg/1 NAA was feasible and the method can be applied in transformation experiments.
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Post-effects of cytokinins and auxin levels of proliferation media on rooting ability of in vitro apple shoots (Malus domestica Borkh.) 'Red Fuji'
26-29.Views:544Rooting ability of in vitro apple shoots of 'Red Fuji' grown on proliferation media with different hormone content were tested at three IBA levels in root induction media. Rooting percentage could be slightly increased with an increase in IBA concentration in proliferation media. The highest IBA concentration (3.0 mg 1-1) in root induction media showed strong inhibitory effect on rooting capacity of in vitro shoots. The highest rooting percentage (95%) could be achieved by shoots grown on proliferation media containing TOP or BA+KIN as cytokinins before rooting.
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Impact of organic and inorganic fertilizers on the growth and yield of cabbage in Ghana
46-49.Views:1091A field experiment was conducted at Dormaa Ahenkro, Ghana, to determine the impact of inorganic and organic fertilizers on the growth and yield of cabbage. The experiment was laid in a randomized complete block design with three replications. The experiment treatment comprises of sole application of poultry manure (PM), NPK 15:15:5, the combined application of poultry manure and NPK fertilizer (PM+NPK) and the control (no fertilizer). Data was collected on the number of leaves, stem girth, plant height, head diameter, head weight and edible head weight. There was a significant difference (P<0.05) among all the treatments. The combined application of PM+NPK recorded the highest values for all the parameters measured. From the results obtained farmers should consider the combined application of PM and NPK to maximize yield on their farm.
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Preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard
19-21.Views:485In this study, we aimed to report a preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard. Results showed that number of symptomatic fruit ranged between 22 and 42 in 2013 and between 25 and 35 in 2014. Number of asymptomatic fruit ranged between 111 and 187 in 2013 and between 119 and 167 in 2014. Disease incidence of fruit ranged between 19.7 and 23.2% in 2013 and between 19.1 and 26.5% in 2014. Disease aggregation index ranged between 0.111 and 0.335 in 2013 and between 123 and 401 in 2014. Three of the four trees showed significant within canopy aggregation of disease for fruit brown rot symptoms in both years. However, the remaining one tree exhibited random patterns during both years. Disease aggregation indicated a disease spread by fruit-to-fruit contact and/or an aggregated
pattern of insect damage.
