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  • Differing effects of grass and polythene mulches on sweet potato productivity in derived savannah agro-ecology
    24-30.
    Views:
    424

    Sweet potato as a food crop that is grown for both its tuberous roots and vegetative parts, the latter of which also serves as animal feed. Its production in the savanna regions can be limited by high evaporative demand, especially where soils with low water retentivity are involved. This work studied the effects of various locally available polythene as mulch materials for sweet potato growth in derived savanna agro-ecology. An experiment was thus conducted at Nsukka, a derived savannah in southeastern Nigeria, with orange fleshed variety of sweet potato. Treatments were polythene mulches of contrasting colours (black, white, red and blue polyethylene), including also organic mulch (dry grasses) as reference and a no-mulch control. The experiment was executed in two successive cropping seasons, and the data averaged and analysed. Agronomic and weed incidence data collection was done fortnightly from 2nd to 10th week of planting. The results showed that the polythene mulches (especially the black one) were better in the control of weeds. Furthermore, the sweet potato yields were affected significantly (p < 0.05) by mulching on the total number of tubers for each plant with grass-mulched plots showing the highest value (36) and transparent polyethylene-mulched plots the lowest (19). Also, total weight of tubers per plant at harvest differed (p < 0.05), with grass-mulched plots showing the highest value (2.39 kg), followed by the control (no mulching) plots (1.62 kg), then the coloured polyethylene mulched plots with the red polyethylene-mulched plots as the lowest (0.64 kg). This study recommends the use of organic mulch or no mulching in sweet potato production in this agro-ecological zone as they produced the best yields.

  • Ornamental plants in Hungary Part I. Protected cultivation
    102-105.
    Views:
    362

    Between 1950-1989, the production and trade of ornamental plants in Hungary was characterised by meeting the demands of the home and that of the Eastern-Block market and by a minimal external trade with the Western countries. After the socio-economical changes in 1989/90, the trade of flowers gradually became liberalised and the Hungarian growers had to face the concurrency of steadily increasing import from the West and from all over the World. This tendency coincided with the physical and mental degradation (outdating) of most of the glasshouses, along with the decline of several former large growers of the communist type (state or cooperative), the appearance and growth of new private companies and the building (rebuilding) of new plastic houses and (mainly second-hand) glasshouses. In spite of the above-listed problems, the production as a whole did not (or only slightly) decrease and/or even an increase occured in many areas mainly in the open-ground production. In 1998, the protected flower cultivation comprised round 110 ha of glasshouses, 180-220 ha of plastic structures and 3-5 ha of frames, with the main crops as follows: cut flowers and cut foliage 220-240 ha; pot plants (with geraniums) 30-40 ha; bedding plants (without geraniums) 20-25 ha; "transit--greenhouses (for redistribution only) 3-5 ha: other (eg. propagation of woody ornamentals) 3-5 ha. The structure of open-ground production was as follows: Total 1150-1210 ha, including: Nursery products: woody ornamentals 880 ha; perennials 10-15 ha; rose bushes 30-35 ha. Other open-ground crops: flower bulbs 50-60 ha; dried flowers 130-140 ha; open-ground cut flowers 25-30 ha; flower seed 30-35 ha; (biennial) bedding plants 10-15 ha.

  • Biological performance of grape varieties in Eastern Hungary
    31-35.
    Views:
    382

    Eurasian and interspecific grape varieties and candidates were inspected for biological productivity in the variety collection of the University of Debrecen at the Horticultural Experimental Station in Pallag. In this paper, data are reported on yields (kg/stock), cane production (kg/ stock) and use-up index of wood yield of each cultivar in the experimental years 2011-2012. From the range of Eurasian (Vitis vinifera L.) white wine-grape qualified cultivars and candidates ’Ezerfürtû’, ’Generosa’, ’Jubileum 75’, ’Müller Thurgau’, ’Zöld veltelíni’,’B-11’, ’CSFT-92’, ’Pintes’, from the range of Eurasian (Vitis vinifera L.) red wine-grape qualified cultivars and candidates ’Kármin’, ’Pinot noir’, ’Alicante Bouschet’ and ’Rubintos’ were highlighted. Promising data of interspecific ’Aletta’, ’Csillám’, ’Kunleány’, ’Orpheus’, ’Refrén’, ’Taurus’, ’Viktória gyöngye’, ’Alföld 100’, ’Reform’, ’RF38/32’, ’Toldi’ white, and ’Dunagyöngye’ ’Pannon frankos’ red wine-grape cultivars and candidates were emphasized. Published data refer only to the biological performance of the cultivars. The thorough evaluation of varieties needs respect to data on resistance to fungal disease and climatic extremities, and other characteristics concerning production technology and oenological parameters.

  • Effect of late spring frost event on nutrition aspects of a sour cherry orchard in East Hungary
    93-97.
    Views:
    1446

    Similarly to 2007, 2011 was also critical year for fruit growers in Eastern-Hungary. Serious frost damage was observed at late blooming period (6 May (T=-1.6°C)) in this region, which caused approximately 60-65% of fruit loss. The aim of this study is to investigate the effects of a spring frost event on nutrient uptake and status of the trees of a sour cherry plantation at Újfehértó. The symptoms of frost were observed visually. This visual observation was confirmed by SPAD readings. The frost affected the macroand micronutrient contents of leaves. It was found that the frost affected the nutrient uptake negatively, but the effect of it was not significantly sometimes. It can be stated that the nutrient demand of trees can be supplied only under even worse conditions.

  • Hazardous element content and consumption risk of 9 apricot cultivars
    61-65.
    Views:
    985

    The heavy metals pollution is one of the problems that arise due to the increased uses of fertilizers and other chemicals to meet the higher demands of food production for human consumption. In order to assess possible health risk of apricot (Prunus armeniaca L.) consumption, levels of Arsenic, Cadmium, Mercury and Lead were determined in fresh and dried samples of "Jumbo cot", "Tom cot", "Gold strike", "Gold bar", "Bergeron", "Bergarouge", "Sweet cot", "Yellow cot" and "Zebra" apricot cultivars. Wet digestion of samples with concentrate HNO3 – H2O2 digester mixture and inductively coupled plasma–atomic emission spectroscopy was used. Highest content of As, Cd, Hg and Pb among all cultivars, were 0.5, 0.04, 1.5 and 0.5mg/kg of dried apricot samples. Fresh fruit samples also contain 0.2, 0.016, 0.6 and 0.2 mg/kg of Arsenic, Cadmium, Mercury and Lead respectively. Daily intake of metals, hazard quotient and health risk index to reveal health risk possibility of dried and fresh fruits consumption were calculate and compared.

  • Nutritional values of traditional open-pollinated melon (Cucumis melo L.) and watermelon (Citrullus lanatus [Thumb]) varieties
    29-31.
    Views:
    541

    The aim of our experiments was to investigate the internal quality parameters of some old melon and watermelon landraces or varieties whether they have any desirable characteristics. Measurements were carried out using 8 different types of melon (Cucumis melo L.) and 8 accessions of watermelon (Citrullus lanatus [Thumb]), which were self pollinated under greenhouse conditions. Besides the determination of dry matter content; soluble solids, titratable acidity of the fruit juices, investigation of carbohydrates, total phenol content was measured by spectrophotometer. Total antioxidant capacities were determined spectrophotometrically using the FRAP method. We found some accesions both among melon and watermelon varieties, which could be characterized by outstanding inner content.

  • Viniculture in the Semi-arid Tropical Region of Brazil
    115-118.
    Views:
    192

    In the semi-arid tropics viticulture was successful according to technologies developed by Hungarian expertise

  • Review of the self-incompatibility in apple (Malus x domestica Borkh., syn.: Malus pumila Mill.)
    31-36.
    Views:
    885

    Apple (Malus x domestica Borkh.) is one of the most important fruit crops showing ribonuclease-mediated self-incompatibility, and no self-compatible apple cultivars are known. Twenty-nine S-alleles were identified in apple and many more incompatibility groups are present compared to sweet cherry. Results from a Belgian, English and a Japanese research group are combined and the S-genotypes of the most important world cultivars are collected. Two different allele labelling system are reconciled and detection methods used in case of the specific alleles are shown. Effects of the resistance breeding programmes are discussed; and scientific efforts involving transgenic technology to create self-compatible genotypes are shown. This review covers the most interesting issues regarding self-incompatibility in apple.

  • Assessment of fruit firmness of pepper using non-destructive physical measurements, in response to different growing and pruning technologies
    59-62.
    Views:
    241

    The European market demands vegetable products of the highest quality and this commercial quality must be maintained till the goods reach the customer. One of these important quality parameters is the fruit firmness of pepper.

    The experiments were aimed at to find out the influence of different growing methods (soil or rockwool-based) and pruning technologies (to 1, 2, 3 or 4 shoots) on the yield and fruit firmness of three pepper varieties (HO F1, Karpia Fl and Pritavit F1) which are common in Hungary. Fruit firmness was measured by the non-destructive impact method.

    On the basis of the results, in unheated forcing the pruning to 1, 2 or 3 shoots can be suggested for all three varieties, as well as the utilisation of rockwool in their growing.

    The non-destructive impact method has been found suitable for testing the fruit firmness of pepper varieties. In the experiments involving different growing mediums pepper stands were found to show significant differences, however the different pruning methods had no significant influence on fruit firmness.

  • Further information to the acclimatization of "in vitro" plants
    54-58.
    Views:
    285

    The experiment was carried out with in vitro propagated 'MM 106' apple-rootstock plantlets. The transpiration of the plantlets was examined, and the changes followed by SEM analysis.

    Data about the transpiration intensity of the acclimatized plants, of its value under different conditions of relative humidity and influenced by the existence of roots, as well as by the degree of acclimatization are presented.

    Leaves were also examined and it was found, that stomata of in vitro developed leaves closed slowly, and the number of stomata of newly developed leaves decreased.

    It is also shown, that in vitro propagated roots, generally, lose their hairs during acclimatization, but these roots are all the same important, as new roots of full value develop out of them.

     

  • Importance of boron in fruit nutrition
    39-44.
    Views:
    1710

    Boron (B) is an essential micronutrient in plants especially in fruits. Despite of this fact there are very few information about its application, uptake and symptoms in Hungarian fruit growing sector. Nowadays we should consider the effects of weather conditions on soil B availability increasingly according to the climatic anomalies. Identifying of internal and external symptoms of plant parts (leaf, fruit) is help for growers to recognize the deficiency and excess symptoms in time. Methods and application rates of boron fertilization provide further information for growers to achieve qualify-oriented fruit growing among Hungarian conditions. The aim of this minireview is to focus on the importance of boron in fruit nutrition.

  • Cost and profit conditions in the Hungarian intensive apple production
    65-68.
    Views:
    446

    In this study I investigated the cost and profit conditions and the efficiency of intensive, qualitative apple production on the basis of a data collection carried out in ventures of high standard production. I concluded that the intensive apple production has an extremely high cost requirement, the production costs are approximately 1500 to 1600 thousand HUF per hectare. In an average case, a production value of 2000 thousand HUF per hectare may be reached, which may fluctuate in a wide interval during the years. Considering the above mentioned, a net profit of 400 to 500 thousand HUF may be realized in one hectare. It should be highlighted that regarding the present marketing conditions, realizing the appropriate profit may be expected only by producing 30 to 40 tons per hectare yields and 80 to 90% food quality ratio.

  • Storability of paprika varieties measured by non-destructive acoustic method
    49-53.
    Views:
    241

    During our experiments, the storability of paprika (Capsicum annuum) samples was measured by a non-destructive acoustic method. The aims of our work were the determination of the applicability and reproducibility of the acoustic stiffness method for paprika, the investigation of the optimum measuring conditions. In order to compare the main paprika varieties regarding shelf-life, our further aim was to follow the softening phenomenon or textural changes (i.e. changes in stiffness) of different paprika varieties measured by the non­destructive acoustic stiffness method. Five different varieties of paprika grown in hydroponics growing system were used for the measurements. All paprika varieties were stored at 20 °C for two weeks. Samples were tested on every 2nd or 3rd day. The acoustic method was found to be suitable to follow the softening of paprika samples. The characteristic frequency of the acoustic signal could be well detected and clearly separated from the other vibration peaks. Tapping the top of the paprika was observed to give a clearer and less noisy signal compared to the signal obtained by tapping the sample's shoulder. The acoustic results showed the same tendencies with regard to softening during storage as the impact method showed in our previous experiments.

  • Interaction impact of drought stress, nutrient-deficient water, and seed-borne pathogen (Alternaria alternata) on germination and vigor of two tomato varietiesination and vigor of two tomato varieties
    48-53.
    Views:
    317

    Tomato (Solanum lycopersicum) is considered one of the leading vegetable plants in the world. This study evaluated the germination and vigor capabilities of ‘Marmande’ and ‘Kecskeméti Jubileum’ varieties under different conditions, including drought stress, nutrient-deficient water, and the effect of seed-borne disease caused by Alternaria alternata when prime and non-prime with salicylic acid. The experiment was conducted in the laboratory in 2023 at Agricultural and Food Sciences and Environmental Management Faculty of University of Debrecen. Results indicated that the ‘Kecskeméti Jubileum’ variety exhibited a superior strength to ‘Marmande’ in the seedling’s dry weight, seedling growth rate, and vigor index under nutrient-deficient water. Nevertheless, when applying levels of drought, the ‘Marmande’ variety had a higher viability rate of 62.5% in comparison with another variety, and the germination rate of the two tomato varieties is at 85.5% in 5% concentration but decreased progressively when exposed to a higher drought concentration of 10%. When using 3 ml of salicylic acid during germination stages, the seedling vigor index of ‘Marmande’ shows a greater index at 165 compared to ‘Kecskeméti Jubileum’, just 108 under the infection of Alternaria alternata.  The results of the examination of drought stress, and the effect of Alternaria alternata, one cause of seed-borne pathogens, showed that the percentage germination and vigor ability of the ‘Marmande’ variety performed better than ‘Kecskeméti Jubileum’ under the same conditions.

  • Comparative study of sour cherry samples for their anthocyanin content measured by tools of the laboratory and by portable equipments
    29-31.
    Views:
    947

    The inner quality of fruits depends on many components. The analysis of pharmacologically important fruit species as the sour cherry consumed as a common food is an expensive task, mostly requiring an equipped laboratory. In sour cherry, one of its valuable components, the anthocyanin content deserves special attention. A portable analytical implement, which has been developed by our team measures the anthocyanin content on a scale of 1024 PharMgrades. The system is a member of the UVEX family of implements working with a microprocessor and performs the measurement quickly. The portable variant (UVEX-ML-1) does not require much training, the laboratory variant (UVEX UL-1) performs a large quantity of tests under modest laboratory conditions. Preparation of the samples is easily done, the necessary elements are available in the trade. The reagent is easily stored and dosed. The system was tested and proved to be of sufficient precision and the result showed acceptable variance according to the checks performed by a spectrophotometer in the laboratory.

  • Experimental results of the effects of Hungarian climatic conditions to German disease-resistant industrial apple varieties
    53-56.
    Views:
    207

    In the recent years, several disease-resistant apple varieties appeared through the modern breeding technologies. These varieties can be grown with low usage of pesticides, which mean not only environment friendly fruit growing, but the production costs are also lower. In Eastern-Hungary — it is one of the main apple growing regions — a new apple growing structure started to form by the investment of the German Wink Ltd. — several resistant apple varieties were brought from Germany. 'Resistant' refers genetic resistance that usually transferred from the genome of wild apple species. But the fruit of these apple species is not only resistant to diseases, but its quality is poorer, too. In Germany the Re-apples are grown only for the processing industry. Due to climatic circumstances in Eastern Hungary, the first experiences showed better parameters during laboratory measurement, the fruits have more beautiful view, shape and inner characters than usual industrial apples.

    In our paper we discuss the results of sensory (consumer) tests, carried out in Eastern Hungary and in the Budapest-region the data analysis of systematic storing experiments (refraction, flesh firmness, weight loss, etc.) and profile analysis of fresh and stored Re-apples. (In the profile analysis the ProfiSens software [4,5] has been used.)

  • Studies on the drought responses of apple trees (Malus domestica Borkh.) grafted on different rootstocks
    29-36.
    Views:
    1402

    The morphological and chemical changes in the leaves of eight apple varieties grafted on M4, M26 and MM 106 rootstocks were examined during fruit development in non-irrigated plantation. The aim of the experiment was to examine the defensive mechanisms in apple trees under the mild and serious dryness occurring during fruit development. The content of a relative chlorophyll (SPA D value) of the apple leaves on eastern side of the trees was lower than western and southern sides under drought occurring du ring fruit devclop111ent Under sustained drought from first of July to August the SPAD value, the weight of leaves were larger and the stomata density was significantly larger than in the previous periods. There was a close positive significant correlations bet ween SPAD and stoma density both in the group with less sensitive to drought (r=0,8429) and in the large water demanded group (r=0,9209) equally. The rate of increase in SPAD values and the stoma density was slower in the group with drought tolerant, than in the group required good water supply. The varieties being sensitive to water deficite such as Akane. Pink Lady and Red Rome van Well have a l ready responded intensively to short time of drought by the increasing of carbohydrate and antioxidants product ions. At 42 days of sustained drought as soon as the level of carbohydrate in the leaves decreased, the antioxidant s contents of the leaves rised suddenly in the apple varieties with moderate water requirement s as Greensleeves and ldared. Gala apple variety seemed to be able to defend the drought for longer time because there was no change in relatively high level of carbohydrates and antioxidants of their leaves under severe drought during fruit development.

  • Susceptibility of European pear genotypes in a gene bank to pear psylla damage and possible exploitation of resistant varieties in organic farming
    95-101.
    Views:
    1077

    We evaluated 285 pear genotypes (commercial cultivars, ancient local varieties, unnamed local strains, seedlings, wild seedlings) in the largest gene bank of pear in Hungary from the point of view of psylla resistance to explore their possible exploitation in organic farming. We have found some 10 new resistant types (Bókoló körte, Bôtermô Kálmán, Füge alakú körte, Nagyasszony körte, Nyári Kálmán, Rozs nyári körte, Viki körte, Pb-242, Pb-299, 0-632) and 7 highly tolerant ones (Cure-6, Kései Kálmán, Kieffer, Kieffer Éd, Steiner, Téli Kálmán, II. B-3- 6/4, 96-16/5) (Table 1). These made up 3.5 + 2.8 per cent of the investigated genotypes, while 93.7 per cent of them were susceptible to pear psylla damage. Taking earlier and present results into account we can list more than 30 European pear cultivars being resistant or highly tolerant to pear psylla infestation and damage. In fact, the list of resistant and highly tolerant cultivars may serve as a basis selecting pear cultivars fitting to the specific requirements of the organic farming. By the end we can conclude that there is some real hope to exploit some resistant or highly tolerant ancient or local cultivars in organic farming but further investigations are needed to estimate their yield capacity and fruit quality.

  • Recent findings of the tree fruit self-incompatibility studies
    7-15.
    Views:
    471

    This review endeavours to collect all recent and substantial contributions to the quickly deepening fields of tree fruit self-incompatibility studies and hence updating previously published reviews. Studies carried out to discover the molecular basis of gametophytic self-incompatibility are summarized and a newly described model for the solanaceous plants is also outlined. We describe recent findings in all economically important fruit tree crops involving apple, European pear, sweet and sour cherries, almond, Japanese plum, sloe, Japanese apricot, European apricot and peach. Additional DNA sequences are now available for both the pistil and pollen component genes in several species and their molecular, evolutionary or economic implications are discussed in the light of the fruit setting behaviour.

  • Comparative study of some nutritionally important components on different cabbage (Brassica oleracea L.) genotypes
    81-84.
    Views:
    415

    Vegetables of Brassicaceae are especially important because of their inner values, which play a provably positive role in curing and preventing diseases. Cabbage (Brassica oleracea L. convar. capitata provar. capitata) is still today one of the most frequently consumed green vegetables, which is known to brake the absorption of carcinogenous substances and to obstruct cell mutation. Different cabbage cultivars were compared growing under the same circumstance. The level of different carbohydrate fractions and some biologically active quaternary ammonium compounds were determined. In all cases we analyzed the antioxidant capacity of samples characterizing the free radical scavenging capacity.

    Clearly detectable significant differences were found between the varieties tested, which appeared suitable for selecting the most precious varieties for human nutrition.

  • Production of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) gene
    104-107.
    Views:
    417

    Dianthus chinensis and Dianthus caryophyllus varieties were tested for shoot regeneration from leaf and petal explants and transformed with Agrobacterium tuniefaciens strains (EHA 105 and LBA 4404) harbouring an apple derived ACS cDNA in antisense orientation in order to reduce ethylene production and influence the ethylene dependant traits in carnation. After transformation regenerating shoots were selected on MS medium containing 50-75-100-125-150 mg/1 kanamycin and supplemented with 1 mg/1 BA, 0.2 mg/1 NAA. Transgene integration was proved by PCR analysis with npt II spcific primers followed by Southern hybridisation of DNA isolated from green shoots on medium containing 150 mg/1 kanamycin. Several putative transformants were subjected to RT-PCR in order to examine the npt 11 expression at mRNA level. Both the transformant and the non-transformant plants were potted into glasshouse to observe the effect of changed ethylene production on flowering time, petal senescence and vase life.

     

  • A critical evaluation of methods used for S-genotyping: from trees to DNA level
    19-29.
    Views:
    424

    Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.

  • Effect of boron fertilisation on the flavour of carrots - Applicability of organoleptic analyses to carrots
    51-54.
    Views:
    212

    Foliar boron fertilisation has had positive influence only on carotene content. Results were evaluated with chemical analyses and sensory tests. It has been observed that sensory tests are able to detect bitter flavour and also to measure its interaction with sweet flavour. Sensory tests for evaluation are generally used in the EU, also in the case of fruits and vegetables. With the aid of students and staff having received training and acquired practice, our University has the possibility to complete chemical analyses in such a way that satisfies modern demands, as well as facilitating the sale of the products on foreign markets.

  • Dynamics of the uptake of nutrient elements from the medium of in vitro cultured apple rootstocks
    50-53.
    Views:
    312

    Cation uptake of J-TE-F apple rootstocks propagated in vitro was studied by the analysis of culture medium. Test-plants were grown on liquid medium under different light regimes. Samples for tests were taken twice a week.

    Media without plants served as controls. The analysis of those showed, that only the uptakeable iron-content changed depending upon light treatment. The concentration of all other cations was considered unaltered.

    As a result of analysis, it could be established, that elements present in the media were taken up in different rates by plantlets: Cu, P and Zn were utilized totally, but only 50% of K and 20 to 40% of Ca and Mg were taken up under the light treatments applied.

    The dynamics of the uptake process was also observed. It was registered that they differed in the case of some cations. So Ba was utilized at the beginning of subculture, others for example B in the later phases. Some elements disappeared unevenly so K, P but the whole quantity is taken up during subculture.

  • Evaluation of the quality of Sorbus fruits belonging to different species
    37-41.
    Views:
    269

    The interest in wild growing fruits was increased considerably by the pharmaceutical industry, the cosmetics as well as by the food industry. (Stefanovits-Bányai et al., 2004). Elderberry (Sambucus nigra L), sand thorn (Hippophae rhamnoides L.), cornet (Cornus mas L.), dog rose (Rosa canina L. bearing hips) and the Sorbus species (Sorhus ssp.) have been well known medicinal and/or ornamental plants since long. Recently, precious substances have been detected in their fruits, which are indispensable in healthy foods. Several species and micro-species of the genus Sorbus are components of the native flora of Hungary, and the fruit of some of them have been consumed traditionally, however, they are preferably considered as ornamentals. The nursery of Alsótekeres (Balatonvilágos) maintained some 16 clones of Sorbus species, which are mainly apomictic "micro species" of. the collection. In 2003, a comprehensive analysis of sorb fruits born on apomictic micro species was initiated in order to find those, which will be suitable to establish plantations. It turned out that considerable differences exist between the fruits of individual taxa, however, it is largely influenced by seasonal effects. According to physical measurements, a scale of mean fruit masses could be established. As for chemical ingredients of the fruits, those are of special interest, which are involved mainly in anti-oxidant activities of the organisms (calcium, potash, phosphorus, copper, magnesium).

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