Vol 12 No 2 (2006)
Cikkek

A critical evaluation of methods used for S-genotyping: from trees to DNA level

Published April 19, 2006
J. Halász
Corvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44., Hungary
A. Hegedűs
Corvinus University of Budapest, Faculty of Food Science, Department of Applied Chemistry, 1118 Budapest, Villányi út 29., Hungary
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APA

Halász, J., & Hegedűs, A. (2006). A critical evaluation of methods used for S-genotyping: from trees to DNA level. International Journal of Horticultural Science, 12(2), 19-29. https://doi.org/10.31421/IJHS/12/2/631

Abstract

Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.

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