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Studies on the essential oil of different fennel (Foeniculum vulgare Mill.) populations during onthogeny
27-30.Views:490In the recent studies two Foeniculum vulgare Mill. populations (Hungarian and Korean) had been studied in open field trials in 1997-98 in Budapest for the identification of their essential oil characteristics. The essential oil accumulation level as well as the composition of the oil were studied during ontogenesis and at 6 phases of the umbel development from budding to overripening.
In the vegetative phases, the accumulation level of the essential oil was higher in the Hungarian genotype. In the leaf rosette stage, anethole is the main compound in both genotypes (40-96%), except the root of the Korean populations which contains 54% dillapiol. The accumulation level of anethole is slightly decreasing, while dillapiol is slightly increasing during the ontogeny. a- and [3-pinenes are characteristic compounds of the leaves, especially in the Korean genotype (10-11%). Before seed setting, fenchone was present in a considerable amount (7.5%) only in the umbels of the Hungarian genotype.
During the generative development, the maximum values of essential oil content are reached at the milky fruit stage (10,11%) in the Hungarian. and at the green fruit stage (7.1% ) in the Korean type, while the composition of the essential oil changed only slightly. The ratio of fenchone is increasing after flowering and being stable during ripening. Anethole varies to a smaller extent in the umbels, only.
We proved, that the dinamics of essential oil accumulation and the oil composition may vary according to genotype. Based on our results, the Korean population is characterized in the system of Bernáth et al. (2) as a high anethol-low methyl chavicol chemoform of the anethol chemovariety (fenchone<15%; anethole>68%; methyl chavicol< 3,2%).
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The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time
27-29.Views:763Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.
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Nutritional and seasonal requirements for callus growth in Taxus baccata
111-114.Views:205Callus cultures derived from young stems of two varieties of Taxus baccata cv. aureovariegata (genotype I) and Taxus baccata L. (genotype II, III) were induced. Gamborg's B5 medium was supplemented with different concentrations of auxin (2,4-D) in combination with cytokinins (kinetin or topolin) and with a phenolic-binding compound (PVP) to prevent callus darkening and growth inhibition. Stem explants displayed different responses to in vitro culture depending on plant genotype and on the season. Genetic variability was observed in the growth rate of calli initiated from all three genotypes of the same Taxus species. We found the best growth of callus cultures originated from the genotype ill in defined media. After the first subculture the majority of the cream-coloured primary callus turned brown and ceased its growth. However, the long-term culture was initiated.
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An investigation of genotype-environment interaction and stability for pea (Pisum sativum L) seed yield
53-55.Views:213Eighteen pea breeding lines and three check cultivars were tested in two years with and without irrigation. The linear regression can explain only a small part of GEI so the use of regression technique was not possible for interpreting the data. In this case the ecovalence, stability variance and superiority measure stability parameters cannot describe properly the genotype's response. With the AMMI method it is possible to group properly the genotypes according to their response.
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Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia
74-83.Views:363Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.
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S-locus genotyping on stone fruits in Hungary: a review of the most recent achievements
39-43.Views:377Central Europe can be taken as a geographical and historical connection zone between the western growing countries and Asian gene centres of Prunus tree fruits. The determination of the S-genotype of stone fruit (mainly almond, plum, cherries and apricot) cultivars and landraces has both practical and theoretical significance. Our group has allocated complete S-genotypes for more than 200 cultivars and selections of almond, Japanese plum, sweet cherry and apricot. Among Eastern European almond cultivars, two novel cross-incompatibility groups (CIGs) were identified. S-alleles of a related species were also shown in P. dulcis accessions; a fact seems to be indicative of introgressive hybridization. Our results with Japanese plum clarified and harmonized two different allele nomenclatures and formed a basis for intensive international studies. In apricot, a total of 13 new S-alleles were identified from Eastern European and Asian accessions. Many Turkish and North African cultivars were classified into new CIGs, III–XVII. Results suggest that the mutation rendering apricot self-compatible might have occurred somewhere in south-east of Turkey and we were successful to confirm the presumed Irano-Caucasian origin of North African apricots based on the geographical distribution of S-alleles. In sweet cherry, new alleles have been identified and characterized from Turkish cultivars and selections. In addition, wild sweet cherry and sour cherry S-alleles were also shown indicating a a broader gene pool in Turkey as compared with international cultivars. We also used S-genotype information of Ukrainian sweet cherry cultivars to design crosses in a functional breeding program. Our results exhibit an increased number of S-alleles in tree fruit accessions native to the regions from Eastern Europe to Central Asia, which can be used to develop S-genotyping methods, to assist cultivation and draw inferences for crop evolution.
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Self-incompatibility in pears (Pyrus communis L., Pyrus serotina Rehd. and Pyrus ussuriensis) Review
87-91.Views:430Self-incompatibility system and allele pool of three different pear species, European pear (Pyrus communis), Japanese pear (P. serotina) and Chinese pear (P ussuriensis) are displayed. Several inconsistencies and the absence of the harmonization of three different allele series are revealed in the European pears. By collecting data from several reports eight incompatibility groups of Japanese pear cultivars could be established. A self-compatible genotype is analysed in details and shown to be a stylar-part mutant. As Japanese pear was the first fruit tree species from which S-ribonucleases were identified, the history of S-genotyping from the beginning to the latest achievements and technical developments can be also monitored from the experiments enumerated. In Chinese pears, seven S-alleles and one incompatibility group could be identified.
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Preliminary evaluation of breeding perspectives of Ukrainian sweet cherry cultivars: nutraceutical properties and self-incompatibility
7-11.Views:1009Some traditional sweet cherry cultivars of Ukrainian origin may represent perspective material for Hungarian cherry breeding. A total of eight cultivars analysed represent great diversity in several phenotypic traits including fruit ripening time or fruit flesh colour. Considerable differences in the anthocyanin content may result in different antioxidant capacity of fruits. In the present study, we used ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) assays to characterize fruits’ nutraceutical properties. These values were compared with the respective values measured for eight commercial cultivars grown in Hungary. The average of FRAP and TPC values was higher for the Ukrainian cherries compared with commercial cultivars suggesting they might be included in functional breeding programs. Since, cherry is a self-incompatible species, the determination of S-genotype is required for both breeding and successful cultivar association in commercial orchards. Complete or partial S-genotypes were determined for 5 and 3 cultivars, respectively.
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Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis
69-72.Views:438Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of some Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S1S2) and `Harcoe (S1S4) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.
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Effects of different cytokinins on the shoot regeneration from apple leaves of 'Royal Gala' and 'M.26'
69-75.Views:457The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron, or 6-benzylaminopurine, or meta-topolin, or zeatin, or kinetin, or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg 1-1. Effects of 'these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN). Organogenetic index (0I) calculated from these data was used for the evaluation of efficacy of cytokinins. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, 01: 7.5) was achieved in `Royal Gala' by using TDZ in concentration of 0.5 mg 1-1 (2.271,1M). There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins, namely N9-ribosides applied in less concentration than nonribosides have the same or best regeneration effects except for 6-benzylaminopurine riboside. However, similar relationship could not be detected in the case of 'M.26'. SN was the highest (3.22) using 6.5 mg 1-1 (18.2011M) 6-benzylaminopurine riboside or 8.0 mg 1-1 (21.44 µM) meta-topolin riboside (3.18). SN was not significantly lower (3.12) by using 2.0 mg 1-1 (9.08 1M) TDZ, however, OI was about half as big (0.63 compared to 1.29 or 1.74 with 6-benzylaminopurine riboside or meta-topolin riboside, respectively). 'Royal Gala' had higher organogenetic ability, than `M.26': 3.5-fold higher shoot number per explant and more than 4-fold higher organogenetic index was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26' and if explants of 'Royal Gala' were further cultured with 3 weeks, SN increased from 11.08 to 24.42 on TDZ-containing regeneration medium, which might suggest higher organogenetic ability, too.
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Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids
39-45.Views:361Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated.
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A critical evaluation of methods used for S-genotyping: from trees to DNA level
19-29.Views:426Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.
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Water consumption of the wine grape varieties Kövidinka K.8 and White Riesling B.7
25-30.Views:243In the Carpathian Basin Kövidinka and White Riesling are promising wine grape varieties. As in the region continental climate dominates and dry years are not uncommon it was natural to study the water requirement and consumption of the two varieties. Morphological characters affecting transpiration were observed including leaf area, hairiness, number and type of stomata. The amount of water transpired per unit leaf area and time and rate of water consumption were measured in a model trial in cuttings with known water supply.
The water consumption of vine cuttings depends on varieties and is determined by the genotype but it is also affected by environment. Kövidinka requires little water and uses it to its advantage White Riesling requires more water and uses it rather lavishly. The results of our model trial could be introduced directly into viticulture practice.
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Recent findings of the tree fruit self-incompatibility studies
7-15.Views:473This review endeavours to collect all recent and substantial contributions to the quickly deepening fields of tree fruit self-incompatibility studies and hence updating previously published reviews. Studies carried out to discover the molecular basis of gametophytic self-incompatibility are summarized and a newly described model for the solanaceous plants is also outlined. We describe recent findings in all economically important fruit tree crops involving apple, European pear, sweet and sour cherries, almond, Japanese plum, sloe, Japanese apricot, European apricot and peach. Additional DNA sequences are now available for both the pistil and pollen component genes in several species and their molecular, evolutionary or economic implications are discussed in the light of the fruit setting behaviour.
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Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods
37-42.Views:309Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.
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Nutritional quality, fruit shape and relationships among exotic and local Capsicum pepper genotypes in Uganda
33-39.Views:535Twenty-one hot pepper genotypes comprising of local (15) and exotic (6) types (C. annuum, C. frutescens and C. chinense) were characterized for selected fruit traits after propagation in a glasshouse at the Makerere University Agricultural Research Institute Kabanyolo in Central Uganda using a completely randomized design with three replicates. Ripe fruits were harvested and analyzed; traits evaluated were all significantly different at P<0.05 with variations in quality attributes. The genotype OHA-B305-10 had the highest ascorbic acid content (128.86 mg/100 g) and is recommended for improvement of both local and exotic genotypes targeting the fresh market. Genotypes CAP0408-12 and UG2 WE0511-22, with highest total soluble solids (16.17 ºBrix) and dry matter content (28.59%), respectively should be used in improvements for industrial use or processing to products such as chilli powder or flakes. BRS-M205-04 with highest titratable acidity (1.04%) can be used in enhancing shelf life of genotypes with low titratable acids as well as for the fresh market. In spite of the intraspecific relationships among genotypes, significant differences were observed in their quantitative traits. These genotypes will, therefore, be useful in improving the quality of hot pepper fruit in Uganda.
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Review of the molecular background of self-incompatibility in rosaceous fruit trees
7-18.Views:442This review gives a presentation of the gametophytic self-incompatibility system in the roscaeous fruit trees. Studies to discover the pistil (S-ribonucleases) and pollen-part components (F-box molecules) are summarized and models for the self-incompatibility reactions as well as their molecular background are discussed. We describe how mutations within the S-RNase or F-box genes can contribute to the transition from self-incompatibility to the self-compatible phenotype in many fruit tree crops. The current state of the arts is compared to the information obtained in other plant species possessing similar incompatibility system.
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Floral biology of medicinal plants II. Lamiaceae species
137-140.Views:620Several species in the Lamiaceae family are therapeutically significant medicinal plants. Such as sage (Salvia officinalis L.), rosemary (Rosmarinus officinalis L.), peppermint (Moldier piperita L.). marjoram (Majomna hortensis Winch), thyme (Thymus vulgaris L.), Origanum spp., hyssop (Hyssopus officinalis L.), Lavandula spp., basil (Ocimum basalicum L.), lemon-balm (Melissa officinalis L.) are regularly produced in Hungary. Nevertheless, data on their floral biology are not satisfactory. This review intends to gather information on the characteristic constitution of flowers and inflorescences, blooming dynamics, pollination mechanism and crossability of some of the most significant medicinal and spice species
Flowers are mainly hermaphrodite, however, also monoeceous ones are turning up: pistillate flowers marjoram, tyme, oregano. In flowering of a plant individual, a basipetal blooming sequence is described. These species have a floral structure fully adapted to pollination by insects, and the majority of them is an excellent honey-plant (lavandel, hyssop, sage, etc.).
Flowers exhibit characteristic proterandry, therefore autogamy is considered to be almost impossible, and geintonogamy as well as xenogamy to be the most characteristic ways of fertilization. However, in several cases self-fertilization proved to be also successful. Crossability among cultivars or species is depending on the genotype combination and usually produces less seed than the above forms of fertilization.
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Inhibition and recovery of germination and growing ability of seedlings under and after osmotic stress induced by polyethylene glycol in 8 pea genotypes
53-59.Views:295Germination and early seedling growth of eight pea genotypes were examined under and soon after different (5%, 10%, 15%, and 20%) PEG treatments. Seeds were germinated on PEG solution for 3 or 6 days and then further germinated and cultured on filter paper moistened by water for 3 and 6 days. The length and fresh weight of shoots and roots of seedlings were measured and used for evaluation of genotypes. Roots were less inhibited by osmotic stress than shoots similarly to other plant species. The variability among the genotypes was the greatest in the case of shoot growth at 5% PEG treatment and in the case of root growth at 15% PEG treatment. Results suggest that growing responses of genotypes after cessation of stress are more suitable for the evaluation of their osmotic tolerance, than their responses expressed during in vitro stress conditions. Genotypes with relatively high or low osmotic stress tolerance, respectively, could be distinguished with 6 days after recovery from 3-day-long 15% PEG treatment concerning the rate of shoot weight to root weight.
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Evaluation of potential of fruit weight and fruit width and their interrelationships in the set of apricot genotypes
47-53.Views:210Within the period of 1994-1999, variability of fruit weight and width was evaluated in altogether 24 apricot genotypes. It is concluded that the genotype and climatic conditions of individual years are the factors causing variability in these two parameters of apricot fruits. Variability of fruit weight was significantly higher (approximately 3-times) than that of fruit width. When evaluated on the base of their width, 75.0% and 95.8% of apricots were classified into the groups of extra and first quality, respectively. As genotypes with the maximum fruit width (i. e. above 50 mm) the following cultivars were classified: 'Velkopavlovicka LE-6/2', 'NJA-1', 'M 45', 'M-25' and lednicka (M-90-A)'. The value of fruit width (in mm) corresponded with its weight (in g) only within the range of 40 — 45 mm. With the increasing and/or decreasing size of apricot fruit the changes in fruit weight were more pronounced than in those in fruit width. Within the set of genotypes under study, this relationship may be expressed by the equation y = 0.1234 — 7.6605 + 152.76; the corresponding values of correlation coefficient and coefficient of curve determination are r = 0.95-H- and R2 = 0.959.
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Seasonal root development of Cabernet sauvignon grafted on different rootstocks
61-63.Views:357The minirhizotron system gives opportunity to study the root development without disturbing the soil and root. We have found certified differences in root development during the year 2013 among the rootstocks grafted on ‘Cabernet sauvignon’. The number of roots varied according to the rootstocks in different depth of soil layers and also varied the development of ripeness of the root system. We conclude that root system development is affected by soil physical and chemical properties, but differences according to the rootstock genotype on the similar type of soil exist.
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In vitro plant regeneration from immature embryo axis and cotyledons of common bean (Phaseolus vulgaris L.)
93-97.Views:219Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus. It is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility. Recent advances in tissue culture have offered the opportunity to produce cultivars, which could not be obtained by conventional breeding methods. The use of tissue culture and genetic engineering is viewed as a logical approach to improve bean production. Gene transfer techniques will have a great impact on legumes. Although the concept of cell totipotency is widely proved, in vitro morphogenesis has not yet been achieved for a large number of cultivated beans. Regeneration protocols are strongly influenced by the genotype. In tissue and cell culture of beans, the factors controlling shoot morphogenesis and somatic embryogenesis are still unknown. The reported data suggest a possible way for future research.
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Floral biology of medicinal plants I. Apiaceae species
133-136.Views:684The Apiaceae family consists of several species which are well known for their therapeutical use and also as spice plants. Although fennel (Foeniculum vulgare Mill.), caraway (Carton carvi L.), anise (Pimpinella anisum L.), coriander (Coriandrum sativum L.), dill (Anethum graveolens L.), angelica (Angelica archangelica L.) and lovage (Levisticum officinale Koch.) are also economically considerable cultures, data on their flowering biology are rather scarce. This review supports data on the characteristic constitution of flowers and inflorescences, flowering dynamics, pollination mechanism and crossability of some of the most significant medicinal and spice species.
The inflorescence is a compound umbel. Flowers are hermaphrodite, however also monoeceous, mainly male flowers are turning up too. In blooming of a plant individual, a strict sequence is observed. The main umbel is the first to bloom, followed by the different range umbels in order of their range. In their pollination, both wind and insects are considered to be active. For each species, proterandry is characteristic. Although autogamy is considered to be almost unpossible, and geintonogamy as well as xenogamy to be the most characteristic ways of fertilization, several cases of self-fertilization proved to be also successful. Crossability among cultivars or species is depending on the genotype combination and usually produces less seed set than the above forms of fertiliiation.
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Self-incompatibility in plums (Prunus salicina Lindl., Prunus cerasifera Ehrh. and Prunus domestica L.). A minireview
137-140.Views:757Japanese plums (P salicina) and cherry plums (P cerasifera) are diploid species, while European plum (P. domestica) cultivars are hexaploids. Most diploid species are self-incompatible while fertility relations of the hexaploid European plums are variable between self-incompatibility and self-compatibility. About twenty S-alleles and six inter-incompatibility groups and one S-haplotype responsible for the self-fruitful phenotype were described in Japanese plum cultivars, but studies on cherry plums and even on the European plum cultivars are severely restricted. This review is focused on the available information obtained from myrobalans and European plums; and discusses recent hypotheses regarding the putative origin of the hexaploid plums, and thereby indicates the possibility of allele flow between different plum species.
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Inheritance of male sterility in apricot
12-14.Views:219Progenies (total of 1,114 seedlings) from crosses representing all possible genotypic combinations between 4 male-fertile and 1 male-sterile apricot parents were scored for the male sterility trait. Crosses between putative heterozygous normal cultivars yielded 25% of male-sterile seedlings, which supports a previous hypothesis that male sterility is controlled by a recessive allele of one nuclear locus. Crosses between those parents and putative homozygous normal cultivars did not produce any male-sterile tree. Finally, the proportion of male-sterile progeny in crosses between a male-sterile and two male-fertile cultivars depended on the genotype of the male parent. When it was heterozygous approximately 50% of the progeny was sterile, whereas when a homozygous fertile parent was used, no male-sterile progeny was obtained. These results confirm a previously proposed model, in which the male sterility trait in apricot is controlled by a single recessive gene.
