Vol 6 No 4 (2000)
Cikkek

Nutritional and seasonal requirements for callus growth in Taxus baccata

Published August 23, 2000
K. Brunáková
Department of Experimental Botany and Genetics, Faculty of Science, P.J.Safdrik University, Manesová 23, 04154 Kosice, Slovakia
Z. Babincová
Department of Experimental Botany and Genetics, Faculty of Science, P.J.Safdrik University, Manesová 23, 04154 Kosice, Slovakia
E. Cellárová
Department of Experimental Botany and Genetics, Faculty of Science, P.J.Safdrik University, Manesová 23, 04154 Kosice, Slovakia
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How to Cite

APA

Brunáková, K., Babincová, Z., & Cellárová, E. (2000). Nutritional and seasonal requirements for callus growth in Taxus baccata. International Journal of Horticultural Science, 6(4), 111-114. https://doi.org/10.31421/IJHS/6/4/237

Abstract

Callus cultures derived from young stems of two varieties of Taxus baccata cv. aureovariegata (genotype I) and Taxus baccata L. (genotype II, III) were induced. Gamborg's B5 medium was supplemented with different concentrations of auxin (2,4-D) in combination with cytokinins (kinetin or topolin) and with a phenolic-binding compound (PVP) to prevent callus darkening and growth inhibition. Stem explants displayed different responses to in vitro culture depending on plant genotype and on the season. Genetic variability was observed in the growth rate of calli initiated from all three genotypes of the same Taxus species. We found the best growth of callus cultures originated from the genotype ill in defined media. After the first subculture the majority of the cream-coloured primary callus turned brown and ceased its growth. However, the long-term culture was initiated.

 

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