Vol 20, No 3-4 (2014)

Published September 7, 2014


The uses of wolfberry (Lycium barbarum L.) as a fruit in an international breadth of view

Lycium barbarum is known exclusively as an ornamental plant in Hungary, and is planted so, as popular belief deem it a toxic plant. The plant’s fruit receives great respect in countries abroad, thus, due to its favourable content values the Lycium has achieved the title of ‘biological gold mine’. The last couple of years has seen the impo...rt of Lycium shrubs and its corresponding products, dried goods or in the form of various processed products which have been marketed and sold at extreme prices (under name of Goji, Wolfberry, Lifeberry). Our goal was to examine the similarities of the content values of wolfberry found in Hungary and those cultivated abroad. Along with Miklós Józsa the domestic Lycium population was surveyed based on foreign examples, between the years 2009 and 2011. Those defined sweet and largefruited were selected for further investigations to be set into a clone repository. This clone repository– which contains 67 different clones from a number of regions of Hungary – was established in the nursery of Dr. Miklós Józsa, located in the city of Szombathely. The phenological and morphological characteristics and the fruit ripening and quality indicators of plants in the clone repository were investigated. Six ‘best’ clones – selected based on flavour, disease-resistance and vegetative characteristics (plant size, fruit set, yield and fruit size) were analysed based on their content values. The control plant was a cultivar imported by a delivery service, found also in growing. In addition to the results of the selection, the results of the content values of the six selected ‘best’ clones (total soluble sugar content, glucose-fructose ratio, carotene content, FRAP value) is documented in this paper. Further, based on the resulting information the possibilities of the fruit’s utilization are suggested. Significant differences were measured in the vegetative characteristics of the clones (plant size, fruit set, yield and fruit size) and of those of
its inner content values. The resulting properties are considerable in regards of the plant’s introduction into cultivation and also utilization. Based on growing characteristics, more favourable clones were selected than the foreign varieties already in growing. Those clones selected by us have had similar or better content values than foreign breeds. The investigation of these content values is still in progress.

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Hyperspectral data in water stress detection

Remote sensing methods are applied widespread to investigate large land fields. Within these methods the status of certain vegetation can be determined based on the reflectance spectra of the chlorophyll, in order to support agriculture, forestry and the evaluation of soil pollution. The main aims of our study were to determine and validate the... reflectance spectra of fruit tree species, in order to facilitate the identification and evaluation of stressed fruit trees in orchards.

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Preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard

In this study, we aimed to report a preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard. Results showed that number of symptomatic fruit ranged between 22 and 42 in 2013 and between 25 and 35 in 2014. Number of asymptomatic fruit ranged between 111 and 187 in 2013 and between 119 and 16...7 in 2014. Disease incidence of fruit ranged between 19.7 and 23.2% in 2013 and between 19.1 and 26.5% in 2014. Disease aggregation index ranged between 0.111 and 0.335 in 2013 and between 123 and 401 in 2014. Three of the four trees showed significant within canopy aggregation of disease for fruit brown rot symptoms in both years. However, the remaining one tree exhibited random patterns during both years. Disease aggregation indicated a disease spread by fruit-to-fruit contact and/or an aggregated
pattern of insect damage.

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Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars

European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the secon...d intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We  identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.

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The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time

Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. Th...e harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.

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Preliminary results of fruit quality of resistant sour cherry clones in 2014

The richness of Hungarian sour cherry cultivars in the world is unique; they can be consumed in many ways. Sour cherry is a Hungaricum and has excellent fruit quality and nutritional value. Nowadays the demand for chemical-free fruits is increasing. Reducing the number of chemical applications can be achieved most effectively by the use of resi...stant varieties, technological developments and cultivar innovation. The domestic and international sour cherry breeding programs use almost exclusively the resistant ‘Csengôdi’ clones as a basis. They are mainly Monilinia and Blumeriella resistant and have higher dry matter and antioxidant content. These pathogens influence not only the qualitative and quantitative parameters of the fruits, but may play an indirect role in the deterioration of the overall condition of the trees as well. The cultivation of resistant varieties can greatly reduce the pesticide load of the environment, so we can produce fruit with excellent nutritional value with fewer chemicals. Our aim is the expansion of the sour cherry assortment by introducing new  resistant cultivars. With this we can contribute to the spreading of environmentally sound production technologies and supply “super fruits” to the consumers.

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Preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard

In this study, the objective was to report a preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 11 and 20 on shoot and between 9 and 24 on fruit. Number of asymptomatic plant part ranged between 85 and 109 on shoot and betw...een 133 and 206 on fruit. Disease incidence ranged between 13.8 and 17.6% on shoot and between 9.1 and 11.3% on fruit. Disease aggregation index ranged between 0.098 and 0.228 on shoot and between 0.043 and 0.108 on fruit. One of the four trees showed significant within canopy aggregation of disease for shoot powdery mildew symptoms in both years. For leaf powdery mildew, all tree exhibited random patterns in both years.

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Evaluation of the vegetative and generative performance of new apple cultivars in the Nyírség region

In this paper we examine apple varieties, which are planted expansively in the development apple orchards of the Western- European countries, but about its production characteristics we don’t have or just few practical experiences in Hungary. These varieties can be described with regular and high yields, aesthetic and alluring appearance in t...heir origin places, what ensure profitable producing for the growers. Under the Hungarian climate which tends to be often extreme (hard cold winters, late spring frosts, summer drought, heat stress) these varieties may behave differently, so before the substantial planting it is expedient to carry out variety evaluations to avoid the later failures. The place of our experiments is located in the Nyírség representing adequately the ecological conditions of the surrounding areas, so the gained results can be adapted easily in the biggest apple production site of Hungary. Regarding the vegetative and the generative parameters of the 14 examined varieties (’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’, club cultivar) we found significant differences. The ratio of the thickness of trunk and the main branches showed that the trees have an optimal canopy structure in accordance with the Zahn principles, so they are in harmonious growing balance. The ‘Gala’ sport trained to slender spindle produced the highest yields, while the ‘Fuji’ and the ‘Red Delicious’ sport trained to super spindle can be described with the lowest values. All the cultivars reached the eating quality (65-70 mm), from which the ’Red Idared’, the ’Red Jonaprince’ and the ’Red Cap’ produced outstanding fruit size (81-85 mm).

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Comparative economic analysis of superintensive and intensive apple orchards

The objective of thsi study was to compare economic aspects of superintensive and intensive apple production.
According to our results, conclusions and recommendations regarding the establishment of superintensive orchards are the followings: A yield of 60 tons per hectare in the average of the mature years provide a quite late payback, in t...his way yield losses should be avoided in the plantation of such a huge capital requirement. When yield losses happen, which cannot be avoided or may be avoided only in a limited way, up to 65 to 70 tons per hectare yield should be reached even in good years, in order to yield the average 60 tons per hectare in the long run. In this case yield losses from production technological mistakes must not be arisen. Inputs and professional expertise should be used in a maximum way in order to reach yields ensuring profitable production. Investment subsidies may ensure safer return. The return of a superintensive orchard from totally own sources may be risky under the domestic marketing conditions and selling prices, it is strongly uncertain.

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Significance of vegetable and fruit processing industry with a special regard on berries and nuts

The main goal of our paper is to evaluate the economic performance of processing industry and its significance within food industry, furthermore to define economic weight of processed goods made of nuts and berries. Fruit and vegetable processing industry plays a key role in Hungarian food industry: it provides 10% of its production value and r...evenue, its export is outstanding and its export-import balance was positive in the last 15 years. Purchase of berries has been continuously reduced in the processing industry, mostly raspberry and blackberry decreased. Nut products is and important group; their revenue was 12% of the industry’s revenue in 2012. Processed goods made of nuts and berries are high added value products, while the purchased quantity is small, the value of finished products is high, especially in case of nuts.

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Farm economic evaluation of raspberry production

Hungary was considered as one of the most significant raspberry producers in the 1980’ies. The acreage and the produced quantity, however, reflected a decreasing tendency during the past two decades: the 7 000 hectares existing in the year of 1990 reduced to 1 500 hectares, the current territory does not reach the 500 hectares. The annual yie...ld is only 1 to 3 thousand tons. The level of domestic fresh consumption is very low, due to the fact that it is a relatively expensive fruit for Hungarian consumers. The requirement of the processing industry is satisfied by raspberries from mainly Polish and Serbian import. These two countries belong to the biggest raspberry producing countries in the world by producing raspberries of more than 50 thousand tons. Comparing to the Hungarian production costs and yields they are able to transport their products here at a very low price, consequently they hold the prices at a low level. The profitability of the domestic raspberry production is rather unfavourable, production often shows a deficit even in orchards of good standard; furthermore the lack of labour causes an extremely great difficulty, which is an important component of the decline of the production independently from cost conditions.

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Farm economic evaluation of elderberry production

In this present study the efficiency as well as the farm economic advantages and disadvantages of elderberry production are examined. Our objective is to determine the fact that under what conditions the elderberry production may be profitable regarding the present economic and market situations. Our analysis was carried out by a simulation mod...el based on a farm-level data gathering in production enterprises. The total investment cost of an up-to-date, elderberry orchard of traditionally cultivated without irrigation is between 1000 to 1700 thousand HUF per one hectare and turning to productivity is expected within 4-5th years. These orchards are able to produce yields of 8,0 to 9,0 tons per one hectare in the average of the productive years, which makes reaching a revenue of 800 to 1000 thousand HUF possible regarding a per kg average selling price of 80 to 110 HUF. By this a net profit of 200 to 400 thousand HUF may be realized in case of a per hectare production cost of 600 to 700 thousand HUF. At the end of the lifetime of the orchard (12-15th year) an internal rate of return of 10 to 4%, an NPV of 1500 to 2000 thousand HUF per one hectare are typical in an average case, and the payback may be expected in the 6th to 8th year. From the farm economic aspect the elderberry may be considered as an extensive sector, which advantages are low capital and labour need, early recovery, good-acceptable profit on capital and cost to profit ratios, but its disadvantage is low per hectare profit comparing to intensive fruit species and orchards. In this way in general farms of capital-extensive and avoiding risks choose elderberry production.

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Seasonal root development of Cabernet sauvignon grafted on different rootstocks

The minirhizotron system gives opportunity to study the root development without disturbing the soil and root. We have found certified differences in root development during the year 2013 among the rootstocks grafted on ‘Cabernet sauvignon’. The number of roots varied according to the rootstocks in different depth of soil layers and also va...ried the development of ripeness of the root system. We conclude that root system development is affected by soil physical and chemical properties, but differences according to the rootstock genotype on the similar type of soil exist.

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Polyphenol- and anthocyanin content changes effected by different fermentation- pressing and aging technologies

Different grape processing, fermentation and aging technologies were compared in our study on the white wine-grape variety Grüner Veltliner between 2012 and 2014 in Hungary,Cserszegtomaj. The vines are grown on brown forest soil on dolomite bedrock, stocks were planted 3x1 m row and vine space, respectively in our experimental area. The soil h...as slightly alkaline pH, the orientation of the vine rows are East-West. The training system is modified Guyot cordon, with 1 m trunk height and cane pruning method. After the harvest half of the yield has been put into the de-stemmer crusher before pressing while the other half has been pressed immediately (whole bunches). From the filtered and bottled wine anthocyanin, and polyphenol content was measured in 2013 and 2014. Another enological technology testing experiment has been set on aging of Grüner Veltliner in 2013. The wine was fermented with addition of fine lees from juice sedimentation. Traditional (racking only), battonage and fast ready-made aging technologies have been set together, each treatment in three replicates were observed.

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Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material

Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature... for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.

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Effect of the terroir and the harvest time on the Kékfrankos grape berries visual and physical parameters in Eger (Hungary)

In this research, the effects of the “terroir” and the harvest time on the most important physical properties of the berries of Kékfrankos (Blaufrankish) grape variety were examined in 2010 and 2011. The results of the experimentation shown, that the maturation processes can be well described by different physical parameters of the grape b...erry. The terroir and harvest time effects were investigated in two different vintages and four different territories. It can be concluded, that these physical parameters change significantly during the ripening period, but trends differ between vintages and regions.

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Effect of the terroir and the harvest time on the Kékfrankos grape berries sensorial properties in Eger (Hungary)

In this research the effects of the production site “terroir” and the harvest time on the most important organoleptic properties of the berries of Kékfrankos (Blaufrankish) grape variety were observed. The follow-up of the maturation process in a given parcel can effectively supplemented by the results of sensorial evaluations of the grape... berries. In most cases, the tasters found significant differences in sensorial characteristics of the berries due to the harvest time, although the trends of each parcels were different. Significant differences were frequently found in the result of the observed terroirs.

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Optimization of sample preparation for determination of antioxidant parameters from one grape berry

Berry quality is an important issue in wineprocessing, however evaluating characteristics in a single berry level is very complex. The Phenolic compound analysis is more challenging because of the limited amount of material and a mixture of skin, pulp and seed. No clear protocol exists for evaluating these compounds from single berries. The aim... of our investigation was to develop such a protocol. Single whole grape berries samples were homogenized in 20 replicates by 5 different methods. The most effective method was when berry was placed in a mortar and was crushed with a pestle under liquid nitrogen until seeds were clearly visible. Seeds were then taken from the mortar and crushed between two stainless steel plates with a hammer, and returned to the mortar without residues. Homogenization continued until the sample had a powdery appearance. The homogenized samples were taken for further analysis such as total polyphenols, total anthocyanin contents and total antioxidant capacity. Our results demonstrate that single berries could be processed and that the homogenates were suitable for taking chemically uniform subsamples.

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Sustainable food production with greenhouse technologies

The greenhouse fruit and vegetable production is one important area which supports sustainability. To achieve
sustainable economy and growth, the given natural resources have to be used in a smarter, renewable way in order to avoid
depleting them. New technologies and new methods are developed and implemented to utilize resources in more ...optimized way.
Sustainable food supply is essential globally for the world, however it has to be managed and achieved on local levels. We present
the greenhouse production market restructuring with new players. What kind of difficulties arise in the open field vegetable
production and what benefits can be realized by the customers and producers from the covered greenhouse technology in the
continental and desert climate? What are the technical boundary conditions to establish and operate greenhouse production in
different regions and what are the benefits realized from local food production? As an example, we analyse Qatar’s energetics,
climate conditions and food resources, Qatar’s food supply process and its barriers. We will show how the sustainability and
food safety appear in Qatar’s National Strategy Plan.

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The effect of Ferbanat L nano-fertilizer on the growing of Petunia x grandiflora ’Musica Blue’

During our experiment, the effect of Ferbanat L concentrations were examined by Petunia x grandiflora ‘Musica Blue’ production. The leaf and shoot length, number of flower buds, diameter of flowers, and the date of appearing the first buds were measured. The solution of Ferbanat L in 0.1 % concentration was the most effective on shoot lengt...h (21.7 cm). Remarkable increase was observed by the other treatment groups as well comparing to the control group (5.6 cm), the plants treated with solution of Ferbanat L 0.2% (16.5 cm) and 0.3% (14.4 cm) had significant effect as well. The nano-fertilizer had not positive effect on the other examined parameters as leaf and flower size, number of flower buds or chlorophyll content. The culture period shortened five days.

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Innovative research of ornamental plants in University of Debrecen (2001–2014)

In the University of Debrecen started in 2001 related and biotechnology, genetic researches on climate-changerelated.
This work has already visible signs on the city’s public spaces. The main goal of participants of the program is it that
in Debrecen and in the surrounding communities more adaptable to the variable climate, ecologically... and biologically more
grounded urban ornamental planting are preferred in the next decade. The new results and ongoing researches of the decorative
ornamental and biomass plants are an interdisciplinary breeding program.

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In vitro comparative study of two Arundo donax L. ecotypes’ selenium tolerance

Selenium tolerance of two somatic embryo-derived Arundo donax L. ecotypes (Blossom, 20SZ) were compared in in vitro culture. Sodium-selenate (1 – 100 mg L-1) as known the most phytoavailable selenium form and the less studied red elemental nanoselenium (100 mg L-1) were applied as selenium treatments. Basis on the results Blossom ecotype seem...ed to be more sensitive to the sodium-selenate than 20SZ. Inhibiting effect of selenate was effectuated above 10 mg L-1 in case of Blossom, which was manifested in decreased survival rate and growing parameters. Contrast to this 20SZ could tolerate the selenate ≤ 20 mg L-1 without any toxic symptoms. Lower selenate tolerance of Blossom could be explained with higher selenium accumulation. Both of two ecotypes could also uptake and accumulate the red elemental nanoselenium however in much less extent compared to selenate.

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Development of in vitro propagation system for Atriplex halimus L.

Explants excised from adult shrubs were surface sterilized and cultured on Murashige and Skoog (MS) basal medium in the presence of plant growth regulators (PGRs) at different concentrations. A high multiplication rate of 7.2-fold was achieved every four weeks on MS medium supplemented with 4.44 μM BA, 0.49 μM IBA and 0.58 μM GA3. Rooting wa...s achieved with 73% efficiency within 2-4 weeks on agar-gelled MS basal medium free of PGRs. Rooted plantlets were gradually acclimatized to field conditions over 5-6 weeks with 65% efficiency. For in vitro selection for salt tolerance, MS medium was supplemented with increasing concentrations of NaCl ranging between 25 and 1000 mM. This study has demonstrated that in vitro shoots could tolerate up to 600 mM NaCl with optimal growth at 200 mM, while higher concentrations of NaCl affected growth negatively. Growth and shoot number decreased with increasing NaCl concentration with all plantlets died at 1000 mM NaCl.

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Jerusalem artichoke (Helianthus tuberosus L.): A review of in vivo and in vitro propagation

Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement. It is a perennial tuberous plant rich in inulin and is a potential energy crop. During food shortages in times of war Jerusalem artichoke received more attention by scientists and farmers because of its multiple uses ...as a vegetable, medicinal plant, forage plant and source for biofuel. The energy crisis of the 1970s motivated research on Jerusalem artichoke for biofuel as the aboveground plant biomass and the tubers can be used for this purpose. There are different methods to propagate Jerusalem artichoke using tubers, rhizomes, slips (transplants derived from sprouted tubers), stem cuttings, seeds and tissue culture. So, this review was presented to highlight on propagation of Jerusalem artichoke via in vivo and in vitro techniques.

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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)

The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds coll...ected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.

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