Vol. 20 No. 3-4 (2014)
Articles

Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material

Published September 7, 2014
Authors
E. Manduláné Farkas
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
N. Czotter
National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary
R. Lózsa
Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary
T. Dula
DULA Grape & Wine Advisory Ltd., 3300 Eger, Eszterházy tér 9.
I. Ember
Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary
É. Várallyay
National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary
E. Szegedi
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
View
pdf
DOI
https://doi.org/10.31421/IJHS/20/3-4/1139
Keywords
bacteria, fungi, phytoplasmas, polymerase chain reaction, primer sequences, viroids, viruses, Vitis vinifera
Dates
Published date: 2014-09-07
How to Cite

APA

Manduláné Farkas, E., Czotter, N., Lózsa, R., Dula, T., Ember, I., Várallyay, É., & Szegedi, E. (2014). Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material. International Journal of Horticultural Science, 20(3-4), 69–80. https://doi.org/10.31421/IJHS/20/3-4/1139
License

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Crossref
Scopus
Google Scholar
Europe PMC
Abstract

Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Most read articles by the same author(s)