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Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material

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September 7, 2014
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This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Manduláné Farkas, E., Czotter, N., Lózsa, R., Dula, T., Ember, I., Várallyay, É., & Szegedi, E. (2014). Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material. International Journal of Horticultural Science, 20(3-4), 69-80. https://doi.org/10.31421/IJHS/20/3-4/1139
Abstract

Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.