Vol 21 No 1-2 (2015)
Articles

Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR

Published April 12, 2015
N. Czotter
National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary
E. Manduláné Farkas
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
R. Lózsa
Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary
I. Ember
Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary
G. Szûcsné Varga
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
É. Várallyay
National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary
E. Szegedi
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
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How to Cite

APA

Czotter, N., Manduláné Farkas, E., Lózsa, R., Ember, I., Szûcsné Varga, G., Várallyay, É., & Szegedi, E. (2015). Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR. International Journal of Horticultural Science, 21(1-2), 21-30. https://doi.org/10.31421/IJHS/21/1-2./1153

Abstract

Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and  sequencing are also briefly reviewed.

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