Articles
Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR
Published
April 12, 2015
https://doi.org/10.31421/IJHS/21/1-2./1153APA
Czotter, N., Manduláné Farkas, E., Lózsa, R., Ember, I., Szûcsné Varga, G., Várallyay, É., & Szegedi, E. (2015). Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR. International Journal of Horticultural Science, 21(1-2), 21–30. https://doi.org/10.31421/IJHS/21/1-2./1153
Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and sequencing are also briefly reviewed.
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