Vol. 21 No. 1-2 (2015)
Articles

Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR

Published April 12, 2015
Authors
N. Czotter
National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary
E. Manduláné Farkas
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
R. Lózsa
Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary
I. Ember
Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary
G. Szûcsné Varga
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
É. Várallyay
National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary
E. Szegedi
National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary
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DOI
https://doi.org/10.31421/IJHS/21/1-2./1153
Keywords
bacteria, fungi, phytoplasmas, polymerase chain reaction, primer sequences, viroids, viruses, Vitis vinifera
Dates
Published date: 2015-04-12
How to Cite

APA

Czotter, N., Manduláné Farkas, E., Lózsa, R., Ember, I., Szûcsné Varga, G., Várallyay, É., & Szegedi, E. (2015). Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR. International Journal of Horticultural Science, 21(1-2), 21–30. https://doi.org/10.31421/IJHS/21/1-2./1153
License

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and  sequencing are also briefly reviewed.

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