Vol 7 No 1 (2001)
Articles

Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods

Published March 21, 2001
M. Németh
Plant Health and Soil Conservation Station (PHSCS) of MARD, Budapest H-1118 Budapest, Budaörsi út 141-145.
I. Ember
Plant Health and Soil Conservation Station (PHSCS) of MARD, Budapest H-1118 Budapest, Budaörsi út 141-145.
L. Krizbai
Plant Health and Soil Conservation Station (PHSCS) of MARD, Budapest H-1118 Budapest, Budaörsi út 141-145.
M. Kölber
Plant Health and Soil Conservation Station (PHSCS) of MARD, Budapest H-1118 Budapest, Budaörsi út 141-145.
R. Hangyál
PHSCS of County Fejér H-2481 Velence, Ország út 232.
J. Bozsics
PHSCS of County Somogy H-7401 Kaposvár, Guba S. u. 20.
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How to Cite

APA

Németh, M., Ember, I., Krizbai, L., Kölber, M., Hangyál, R., & Bozsics, J. (2001). Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods. International Journal of Horticultural Science, 7(1), 35-41. https://doi.org/10.31421/IJHS/7/1/245

Abstract

Symptoms resembling phytoplasma disease have been observed on peach trees in a seed-source plantation of stone fruits in south Hungary quite recently. In this publication we report on the results of woody indexing of symptomatic peach trees on GF 305 indicator in the field and under greenhouse conditions as well as on molecular studies. Phytoplasma infection detected on GF 305 indicators in greenhouse and field indexing was confirmed by PCR. Nested PCR was conducted using universal primer pairs followed by group and subgroup specific primers for the second amplification. RFLP analysis of nested PCR products was performed using Rsal restriction enzyme. Based on the results of molecular studies it can be concluded that phytoplasmas, belonging to the European stone fruit yellows subgroup (16SrX-B) were identified in peach trees. Further studies on symptomatic peach trees originating from different parts of Hungary are in progress.

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