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• Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods

  35-41.

  M. Németh

  I. Ember

  L. Krizbai

  M. Kölber

  R. Hangyál

  J. Bozsics

  Views:

  137

  Symptoms resembling phytoplasma disease have been observed on peach trees in a seed-source plantation of stone fruits in south Hungary quite recently. In this publication we report on the results of woody indexing of symptomatic peach trees on GF 305 indicator in the field and under greenhouse conditions as well as on molecular studies. Phytoplasma infection detected on GF 305 indicators in greenhouse and field indexing was confirmed by PCR. Nested PCR was conducted using universal primer pairs followed by group and subgroup specific primers for the second amplification. RFLP analysis of nested PCR products was performed using Rsal restriction enzyme. Based on the results of molecular studies it can be concluded that phytoplasmas, belonging to the European stone fruit yellows subgroup (16SrX-B) were identified in peach trees. Further studies on symptomatic peach trees originating from different parts of Hungary are in progress.

• Microsporogenesis of peach (Prunus persica L. Batsch) varieties

  7-10.

  L. Szalay

  B. Timon

  Z. Szabó

  J. Papp

  Views:

  214

  Bud dormancy during winter is a critical factor in peach production in Hungary. The yield is determined basically by the survival rate of flower buds during winter frosts and by their ability to develop normal floral organs. It is important to investigate the genetic basis of slow floral development during dormancy for the purpose of breeding peach varieties with better winter hardiness. The aim of the present research was to examine microsporogenesis in 14 peach varieties during three successive winters in a Hungarian germplasm collection and to study the effectiveness of this method in variety evaluation. There were significant differences in the dynamics of microsporogenesis both between the varieties and between the years. Of the varieties, ‘Mayfire', bred in California, possessed the quickest pollen development rate. The microsporogenesis of `Piroska', a Hungarian local variety, was the slowest. Rapid floral bud development was observed in `Aranycsillag', `Springcrest' and 'Venus'. A medium developmental rate was characteristic of `Babygold 6', Fairlane', `Michelini' and `Red June', while development was slow in 'Champion', 'Early Redhaven', `Redhaven', `Harko' and `Mariska'. Based on these results, the study of microsporogenesis represents a reliable method for the phenological description of peach varieties during dormancy. The application of this method makes it possible to identify varieties and landraces with slow flower bud development, suggesting better winter hardiness.

• Floral biology of tree fruit rootstocks

  153-161.

  K. Hrotkó

  Z. Erdős

  Views:

  152

  The modern nursery industry requires seed sources of a high quality and regular quantity year by year. Besides the seed sources of processed cultivars (Bartlett pear, Shipley, Elberta peach) special seed orchards are planted with selected seed trees producing high quality and genetically determined seed (hybrid seed or inbred lines). Seedlings are still the most common commercial source of rootstocks for stone fruits (almond, apricot, peach, plum, prune and walnut). Although clonal rootstocks are spreading, usage of seedlings is still predominant at stone fruits and nuts. For successful seed production and planning of seed orchard the knowledge on floral biology, flower fertility, pollination, blossom time of trees (selected clone or cultivars) used for seed production is essential. In this field very little systematic research was carried out most of the papers were published in the second half of the 20th century. Our mini review gives an overview on the importance of flower fertility in the mating systems applied in seed orchards, and the research results on floral biology of fruit tree rootstocks propagated by seed (Prunus avium, Prunus mahaleb, Prunus armeniaca, Prunus cerasifera, Prunus insititia, Prunus amygdalus, P persica, P amygdalopersica, Pyrus pyraster, Pyrus communis and Pyrus betulifolia) over the last decades.

• Determination of chlorophyll content in case of peach leaf curl disease (Taphrina deformans) with spectral analysis

  49-52.

  P. Riczu

  Gy. Bíró

  E. Sulyok

  A. Nagy

  J. Tamás

  Z. Szabó

  Views:

  288

  One of the most important conditions of high quality fruit production is the early detection of hytopathologycal infections. The most dangerous disease of peach (Prunus persica) is the Taphrina deformans, which causes serious damages mainly in the years where the weather condition is cool and wet. In this study, healthy and naturally diseased leaves by peach leaf curl were investigated in the laboratory of University of Debrecen, CAAES, Institute of Water and Environmental Management. Both of hyperspectral measurement and the values of spectrophotometer shown the chlorophyll content of diseased leaves were lower than the healthy samples. Based on the experiments the water status of investigated leaves established the infected leaves contained more water in their tissues.