Search
Search Results
-
Virus and virus-like diseases of grapevine in Hungary
25-36.Views:489Viruses and viroids are submicroscopic infectious particles which can cause disease symptoms on grapevine. These parasites are depending completely on the energy metabolism of the plant cell. To enter the host cell plant viruses depend on injuries or on transmission via invertebrates (insects, nematodes, etc.). Viruses are classified by many characters including particle morphology, host range and information content of the genome. At present about 70 viruses including 7 viroids infecting grapevine are known. In single or mixed infections they are potentially detrimental to the quality and quantity of grape production in any growing area of the world. Some viruses can cause severe economic damage in vineyards. In Hungary many important viruses and viroids have been detected in grape. This review summarises characteristics of viruses and the results of detection and characterization of virus and virus like diseases of grapevine in Hungary. The identification of the causal agent, its transmission, geographical distribution and the development of the diagnostic methods are also discussed.
-
Reaction of different Capsicum genotypes to four viruses
61-64.Views:346The objective of this study was to examine the reaction of 44 Capsicum genotypes to common strain of Tobacco mosaic virus (TMV-C/U1), Obuda pepper virus (ObPV), NTN strain of Potato virus Y (PVYNTN) and legume strain of Cucumber mosaic virus (CMVU/246). Reaction (extreme resistance, hypersensitive reaction, latent susceptibility, susceptibility) of the tested Capsicum species/hybrids and breeding lines seemed to be greatly depending on hosts and viruses. Out of the breeding materials 4/99 F2 and IX-8 in to CMV-U/246, while 32.Bogyisz. type, VI-57 ii. 57/83 and V-12=19/98 to TMV-C/Ui showed extreme resistance. Two lines (V-25 F1=32/98 F1 and V-27 in F4=35/98 F4) showed hypersensitive reaction to ObPV. Latent susceptibility to PVYNTN was observed in case of all eleven tested Capsicum genotypes and in case of several lines to TMV-C/U1, ObPV and CMV-U/246. Other breeding materials proved susceptible to the mentioned viruses. Pepper genotypes showing extreme resistance and hypersensitivity could be used for resistance breeding to viruses.
-
Investigation on the transmission of some Tobamoviruses by pollen and seed in pepper (Capsicum annuum L.)
127-131.Views:398Five pepper cultivars were mechanically inoculated with isolates of three Tobamovirus species, viz. the "Gelb" strain of tobacco mosaic virus (TMV-G), the XM-isolate of dulcamara yellow fleck virus (DYFV-XM) and the Nov/H isolate of pepper mild mottle virus (PMMV-Nov/H), respectively. Symptoms caused by the viruses were characterised. The viruses were sucessfully re-isolated from different organs (roots, leaves, fruit parts) of susceptible peppers to test plants. It was estabilished, that the pollen of diseased peppers carried infective virions at least on their surface. Washes of seeds were highly infective, but no infectivity was found after treatment of the seeds with 2% NaOH or 10% Na3PO4. No infectivity of inocula prepared from seed-coats of alkaline treated seeds was established. Infection of young seedlings grown from untreated seeds was demonstrated, while the seedlings came from alkaline treated seeds remained free of infective virus. The possible role of pollen and seed in the epidemiology of Tobamoviruses pathogenic to pepper as well as the importance of seed treatment is discussed.
-
Reactions of some cucumber (Cucumis sativus L.) lines and hybrids to zucchini yellow mosaic virus (ZYMV) and selection of tolerant breeding lines
66-68.Views:327In the past years zucchini yellow mosaic virus (ZYMV) has been appeared as a new pathogen of cucurbitaceous plants in Hungary. It caused severe disease outbreaks on the fields of pickling cucumber hybrids which are highly tolerant to cucumber mosaic virus (CMV). Preliminary inoculation tests have showed that all of the important pickling cucumber hybrids produced in Hungary were susceptible to ZYMV-P. However, a selection of Chinese Long and the breeding line I- KS 10C proved to be highly tolerant. The Fl plants of the crosses between the tolerant lines and the susceptible genotype H I were susceptible to ZYMV-P. F2 populations segregated for susceptible and tolerant individuals at a ratio of 3:1. The results strongly suggest that the resistance to ZYMV in our sources is controlled by a single recessive gene. Inoculations of the ZYMV and CMV susceptible cultivar Budai csemege and the CMV tolerant hybrid Perez Fl with the complex of ZYMV+CMV resulted extremely severe symptoms (strong mosaic and necrotic spotting of the leaves) on both cultivars. On the Chinese Long line, which is tolerant to ZYMV and CMV, respectively, the complex of the two viruses caused mild symptoms. The results show a synergistic pathological effect of ZYMV and CMV on differrent cucumber genotypes. More detailed studies on the interactions among the plant genotypes, viruses and virus strains are needed to develop cucumber hybrids that are highly resistant to the ZYMV+CMV complex.
-
Virus susceptibility and resistance of Hungarian pepper varieties
68-73.Views:318The aim of our study was to examine susceptibility or resistance of 18 pepper varieties to four viruses [tobacco mosaic
tobamovirus (TMV), sowbane mosaic sobemovirus (SoMV), NTN strain of potato Y potyvirus (PVYNTN) and cucumber mosaic cucumovirus (CMV). Out of the 18 varieties, 13 were resistant to CMV infection. Thirteen varieties were susceptible to TMV, while five ones (Dabora Fl, Brill Fl, Feherozon Synthetic, Ciklon Fl, Cecil Fl) showed only local hypersensitive reaction. All of the tested pepper varieties showed resistance to SoMV. Eight varieties (Tuba, Fehérözön Synthetic, Boni, Alba Regia, Korona, Édesalma, Cecil Fl, Star) were found to be resistant to PVYNTN. Out of the examined varieties five (Boni, Alba Regia, Korona, Édesalma, Star) were resistant to three viruses (SoMV, CMV and PVYNTN). Only one (Cecil F1) displayed complex, extreme resistance to SoMV, PVYNTN, CMV and hypersensitive reaction to TMV, therefore this hybrid is very important in pepper breeding and growing for virus resistance.
-
Situation of peach resistance to diseases in Romania
65-69.Views:354The resistance of peach cultivars to the most important diseases caused by fungi, bacteria, viruses and mycoplasms was studied in Romania over two working stages in the period between 1985-2005. The major diseases examined were: Cytospora cincta Sacc., Taphrina deformans (Berk) Tul., Moruluua laxa (Aderh. & Ruhl) Honey, Sphaerotheca pannosa var. persicae Woron., Stigmina carpophila (Lev) M.B.Ellis, Pseudomonas syringae pv. syringae, mycoplasm and plum pox potyvirus. Based on the obtained results, the studied cultivars were classified into resistance groups for the different diseases (very resistant, resistant, medium resistant, sensitive and very sensitive).Based on the results of our study, the following gene sources were chosen following the evaluation of the various genetic material in the peach germoplasm fund, in the climatic conditions of Romania: Cytospora cincta: Cullinan, Cardinal, Hamlet, NJF 3, Onakita Gold, Triumf, "Superba de Toamna", Anderson, Weinberger; Stigmina carpophila: Armgold, ARK 109, Stark Early Blaze, Cardinal, Congres; Taphrina deformans: Madeleine Pouyet, Cumberland, Harbelle, Indian Blood, Sulivan, Victoria, Zafrani, Pekin, Naradnji Ranhii; Spaherotheca pannosa var. persicae: Triumf; Congres; Victoria; Armking; Morton; Regina; Nectared 7; ARK 125; ARK 134; Regina.
-
Phytobiome management in horticultural systems: beyond the rhizosphere toward whole-plant microbiome optimization
85-101.Views:86The plant microbiome is not confined to the soil. Across every anatomical compartment, the rhizosphere, phyllosphere, endosphere, anthosphere, spermosphere, and carposphere, structurally distinct and functionally specialized microbial communities orchestrate processes fundamental to plant health, productivity, and resilience. Yet horticultural science has remained disproportionately anchored to the rhizosphere, leaving the vast microbial landscape inhabiting aerial, floral, seed, and fruit tissues largely unexplored and unmanaged. This blind spot carries profound consequences at a time when global horticultural production confronts an unprecedented convergence of pressures: accelerating climate instability, stringent restrictions on synthetic agrochemicals, mounting soil degradation, and escalating consumer demand for sustainably produced, chemical-free, premium-quality produce. The biological potential embedded within the whole-plant phytobiome to address these intersecting crises remains critically underutilized. A fundamental barrier to progress is the absence of a unifying scientific framework. Existing reviews address plant-associated microbiomes in disciplinary silos, focusing narrowly on rhizosphere bacteria, individual crop species, or single microbial kingdoms, without synthesizing the full cross-compartment, cross-kingdom phytobiome in the horticultural context. No comprehensive framework has yet integrated microbial community dynamics spanning bacteria, fungi, archaea, and viruses across fruit, vegetable, and ornamental crops within a single, coherent, and practically applicable model. This review addresses that gap directly. Through critical synthesis of compartment-resolved phytobiome research across major horticultural systems, we characterize the taxonomic composition, ecological assembly drivers, and agronomic functional roles of microbial communities inhabiting each plant compartment. We demonstrate that phyllosphere microbiomes confer photoprotection and pathogen exclusion; endophytic communities directly modulate secondary metabolite profiles and systemic immunity; anthosphere microbiomes influence pollinator attraction and fruit set; spermosphere communities determine seedling establishment success through vertical microbial inheritance; and carposphere microbiomes govern post-harvest storability and food safety outcomes. We further establish how host genotype, crop developmental stage, management-induced dysbiosis, and climate-driven perturbations collectively shape phytobiome assembly and functional integrity across compartments. Building on this synthesis, proposing to introduce the Whole-Plant Phytobiome Optimization (WPPO) framework, the first integrative, three-tier conceptual model designed specifically for horticultural systems. WPPO encompasses whole-plant phytobiome profiling using multi-omics platforms, identification of functional microbial modules linked to target agronomic traits, and precision compartment-targeted intervention through synthetic microbial communities (SynComs), encapsulated biostimulants, and digitally guided delivery systems integrated with IoT sensor networks and machine learning decision-support tools. Applied across the full crop life cycle, from spermosphere conditioning at seed priming to carposphere biopreservation at post-harvest, WPPO offers a scalable, evidence-based, and technologically integrated roadmap toward substantially reduced agrochemical dependence, enhanced crop resilience, superior produce quality, and the deployment of ecologically precise next-generation biocontrol and biostimulant strategies.
-
Research Note virus transmission by grape phylloxera (Daktulosphaira vittfoliae Fitch)
73-74.Views:231Grape phylloxera (Daktulosphaira vitifoliae FITCH) infests immature roots of both Vitis vinifera L. and phylloxera resistant rootstocks. A capability to transmit viticultural viruses would make grape phylloxera a phytosanitary threat even under conditions where direct damage by the insect is not likely. We tested the hypothesis that phylloxera could transmit grapevine fanleaf virus (GFLV) by planting infected and non-infected vines in common 10 liter pots and infecting roots of the infected vine with grape phylloxera. In this test infection of a previously non-infected plant in the absence of nematode population suggests that grape phylloxera is a vector of GFLV.
-
Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR
21-30.Views:500Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and sequencing are also briefly reviewed.
-
Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material
69-80.Views:511Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.
-
Study on the viruses of Petunia in Hungary
55-59.Views:275Virus susceptibility of 11 varieties of vegetatively propagated petunia against 2 virus strains (TMV-C/U1 and PVYNTN) were examined. Neither resistant nor tolerant varieties of petunia were found. The virus infection of the Petunia genus was examined in Hungary. The most common pathogens were the Tobacco mosaic tobamovirus (TMV) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). Potato X potexvirus (PVX) was isolated in the varieties of trailing petunia for the first time.
-
A complex system for the production of pathogen-free grapevine propagating material
59-62.Views:586The use of pathogen-free planting stock for new vineyard establishment is a key component in the maintenance and expansion of vine and quality table grape production. The success of the necessary changes in the structure of the grape industry is forced by the globalization process, the climate change, the rediscovery of autochton varieties as well as breeding of new tolerant and resistant varieties. The renewal of vineyards largely depend on the availability of planting stocks. Serbia and Hungary found a common interest in establishing pathogen-free stock materials from newly breed resistant varieties and clonal selections of varieties which are traditional in the Serbian-Hungarian border area. During a cross-border cooperation program a complex system for the production of pathogen-free grapevine propagating material was established. Using heat therapy, in vitro shoot tip culture and traditional and molecular diagnostic techniques new pathogen-free stock materials were established from 26 varieties. They have been or will be tested for the presence of most important grapevine viruses, phytoplasmas, as well as bacterial and fungal pathogens. The complex system applying green grafting for indexing on grapevine indicators can shorten the duration of the procedure from 4 years to two-three years.
-
Studies on the Tobamovirus resistance of the pepper (Capsicum annuum L.) cultivar Greygo
71-75.Views:257Resistance of the Hungarian pepper (Capsicum annuum L.) cultivar "Gre.ygo" to Tohamoviruses has been investigated. All plants of the population of Greygo proved to be resistant to tobacco mosaic and tomato mosaic viruses (TMV, ToMV), both represent the pepper pathotypes Po of Tohamoviruses. Individuals of Greygo, however, were found to be susceptible to pathotypes P12 and P123 of pepper mild mottle virus (PMMV). When inoculated with the XM isolate of dulcamara yellow fleck virus (DYFV, pathotype P1) the population of Greygo segregated in resistant and susceptible plants. These results as well as inoculations of the progenies of three TMV resistant plants clearly showed, that besides the resistance allele Li the cultivar Greygo possesses also an another allele. This allele, provisionally marked by L2g behaves like to the allele L2 characteristic to Capsicum frutescens cv. . Tabasco. Determination of the identity of the allele L2g to the allele L2 needs further genetic and pathological informations. Relations between the Tohamoviruses pathogenic to pepper and the alleles of the resistance gene L are outlined for the discussion.
-
The importance of pathogen-free grapevine propagation material in regards to clonal selection and rootstock breeding in Germany
11-13.Views:371High performing propagation material is essential for a reliable and economical production of quality grapes. Apart from genetic aspects pathogen-freedom is of prime importance in propagation material. In particular virus diseases cause major yield and quality losses and reduced longevity. This is also reflected in the current EU legislation, which focuses on the most common and dangerous viruses: GFLV, ArMV, GLRaV-I and GLRaV-III.Apart from these, locally occurring pathogens, e.g. phytoplasms or agrobacterium, are important as well and should not be present in propagation material. There are several ways to develop pathogen-free clones. Starting with already pathogen-free material is certainly the easiest case, but might not be feasible in local varieties with small acreages and limited vine numbers. In these cases the elimination of pathogens is required, either by heat therapy, tissue culture or somatic embryo genesis.
