Vol 13 No 4 (2007)
Cikkek

In vitro multiplication and hardening of grapevine plants in aeriated media

Published October 16, 2007
A. Zok
Corvinus University of Budapest, Faculty of Horticultural Sciences, Department of Genetics and Plant Breeding, H-1118 Budapest, Villányi út. 29-43, Hungary
A. Zielinska
Hidrokultúra 94 Ltd., H-6600 Szentes, Honvéd út 28., Hungary
R. Oláh
Corvinus University of Budapest, Faculty of Horticultural Sciences, Department of Genetics and Plant Breeding, H-1118 Budapest, Villányi út. 29-43, Hungary
E. Szegedi
Research Institute for Viticulture and Enology, H-6001 Kecskemet, POBox 25, Hungary
View:
pdf
How to Cite

APA

Zok, A., Zielinska, A., Oláh, R., & Szegedi, E. (2007). In vitro multiplication and hardening of grapevine plants in aeriated media. International Journal of Horticultural Science, 13(4), 15-18. https://doi.org/10.31421/IJHS/13/4/766

Abstract

In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.

Downloads

Download data is not yet available.