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Relation between the germination and infection ratio on Sida hermaphrodita L. Rusby seeds under hot water treatment
117-121.Views:210Sida hermephrodita or virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through between ten and twenty years. Additionally, the plants have a lot of uses and benefits for instance it can use it as a fodder crop, honey crop, ornamental plant in public gardens. It has favorable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in base stage of domestication therefore has a serious disadvantage the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seed/acre instead of 10-20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a disposable ,basically wild Sida population. We examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenus or endogenus infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65, 80 and 95 oC), 29,33 to 46% germinated of the seeds collected from the population of S. hermaphrodita in Debrecen. The average germination for all season was 5-10 % wihitout treatment and rised using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46 %) after the hot water operation in spite of the previus studys (Spooner 1985; Chudik et al. 2010; Doliński R. 2009.). We discovered that there are a distinguished close relationship between the seeds collecting time and the infection, as well as germination percentage. Thus, 2009 season was the most favourable in case of contamination (control:17,33 and 80 oC treatment:0%) as well as germination percent. It could be concluded that, the best season for our findings was 2009 due to autumn harvest of Sida seeds. In our oppinion, the autumn harvesting should be the best time to overcome the problem of the low germination and high infection percentage.
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Effects of Foliar Nutrition on Onion Seed Storage under Modified Atmosphere Packages
93-100Views:209Modified atmosphere packaging (MAP) and controlled atmosphere storage techniques to reduce the oxygen around the food are largely used for the preservation of fresh produce. There have been great technological advances in this area of preservation, particularly as it refers to improving the quality and shelf-stability of highly perishable food products, such as produce. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on onion seeds storage under modified atmosphere packages. Germination percent of seeds decreased in 5°C than storage in room temperature. Germination percent of seeds was gradually decreased with increasing the storage period. Packaging treatments had a significant effect on germination percent of seeds. All the packaging treatments had the higher germination percent than the paper package (control). The highest germination percent after 12 months of storage was recorded for the treatment with non perforated polypropylene in room temperature and polyethylene and non perforated polypropylene in 5°C. Catalase activity decreased with the prolongation of storage period. The non perforated polypropylene package had the highest catalase activity. The treatment with non perforated polypropylene had the highest catalase activity after 12 months of storage in both room and 5°C temperatures. Peroxidase activity of seeds was gradually decreased with increasing the storage period. The highest peroxidase activity after 12 months of storage was recorded in non perforated polypropylene in both storage temperatures.
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Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
79-80.Views:162About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible.
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Investigation on the transmission of some Tobamoviruses by pollen and seed in pepper (Capsicum annuum L.)
127-131.Views:254Five pepper cultivars were mechanically inoculated with isolates of three Tobamovirus species, viz. the "Gelb" strain of tobacco mosaic virus (TMV-G), the XM-isolate of dulcamara yellow fleck virus (DYFV-XM) and the Nov/H isolate of pepper mild mottle virus (PMMV-Nov/H), respectively. Symptoms caused by the viruses were characterised. The viruses were sucessfully re-isolated from different organs (roots, leaves, fruit parts) of susceptible peppers to test plants. It was estabilished, that the pollen of diseased peppers carried infective virions at least on their surface. Washes of seeds were highly infective, but no infectivity was found after treatment of the seeds with 2% NaOH or 10% Na3PO4. No infectivity of inocula prepared from seed-coats of alkaline treated seeds was established. Infection of young seedlings grown from untreated seeds was demonstrated, while the seedlings came from alkaline treated seeds remained free of infective virus. The possible role of pollen and seed in the epidemiology of Tobamoviruses pathogenic to pepper as well as the importance of seed treatment is discussed.
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Seed protectant potential of Mitracarpus villosus and Phyllanthus fraternus extracts on germinative capability of stored food grains
7-10.Views:340Seed storage is an essential post-harvest operation that decides the success of seeds viability and germination in next generation. The study explored Mitracarpus villosus and Phyllanthus fraternus extracts as bio-insecticides seed treatments on stored wheat and green gram seeds viability and germination. M. villosus and P. fraternus plants powder were sequentially extracted with solvents of increasing polarity (Petroleum ether, hexane, ethyl acetate, acetone, chloroform and methanol), concentrated and tested for insecticidal activity by fumigant toxicity. The extract which showed maximum activity, was selected for seed viability and germination test. Five replications each were made for the treatment and the control. The plant extracts strengthen the non-phytotoxic nature of plant products against seed viability and germination. Seeds treated with extracts (50-400 μg seed-1) did not lose their viability as it resulted in successful and normal germination within the range of 90 – 97.67% irrespective of the extracts concentration. While, seeds in control recorded 95.55 and 100% germination which were not significantly different (P>0.05) compared to the treated seeds. Based on findings from the study, potential exploitation of M. villosus and P. fraternus as food grains protectant in insect pest management strategies is recommended for the resource poor farmers. However, further investigations are suggested on biosafety and effects of the extracts on the organoleptic contents of the grains prior to consumption.
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Changes in germination ability during genebank storage at some medicinal plant seeds belonging to the Solanaceae family
51-55.Views:131In the present work we have examined the changes of germination ability of some medicinal plant species belonging to the Solanaceae family (Datura stramonium L., Datura innoxia Mill. and Hyoscyamus niger L.) during 5-6 years' storage period. According to our results, all the three species showed an after-ripening behavior. Potassium nitrate and gibberellic acid increased significantly the rate of germination in the case of Datura species. During the storage period tested (1995-2001), the species maintained their germination ability which is favourably but not significantly influenced by the cooled gene bank conditions. The degree of ripeness affected considerably the proportion of germinated seeds at all the three species. As a consequence of our results we emphasise that propagation with completely ripe seeds is proposed, however, half-ripe seeds developped in an unadvantageous vegetation period can be also utilized in the practice. The effect of vegetation year on stramony seeds manifested in the length of after-ripening period, while in the case of henbane the germination ability was also influenced.
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Effect of physical treatments on germination of Ginkgo biloba L.
31-34.Views:160In our country the maindenhair tree (Ginkgo biloba L.) is raised mainly from seeds, so the aim of our experiments was to determine the most useful generative propagation method. However, some experiments have been conducted earlier connected to the germination of the species, but the comparative control of physical seed treatment was done first time by the authors.
After the statistical evaluation of the results it can be stated that the percentage of germination has significantly increased if the seeds received physical treatment (scalding, mechanical scouring). Hereby the pericarp is getting soft or growing thinner, so the germination of the seed is easier. These treatments are extraordinarily simple, easy to carry out and their effect is very favourable, that is why their use is strongly advised.
Comparing the seeds collected at different times was found that the ability of germination is decreasing proportionally with the time spent in the open field. On the basis of our experiments and of earlier practice in Hungarian tree nurseries, our opinion is that the stratification of seeds is not necessary.
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Industrial-scale plantlet production by seed priming and nursery tray seeding method in Sida hermaphrodita L. Rushby
71-74.Views:221Virginia mallow (Sida hermephrodita Rushby), Malvaceae family, is a perspective perennial herb able to yield a biomass crop through the last two decades. In our previous studies, we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. Following these recognition we modifi ed our technique, in such a way that we fractionated the seeds based on their fresh weight / or relative density before we carried out the treatment. When we fi ltered the fl oating seeds on the surface of water, the hot water treatment was performed considerably better on the sink seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination). From all nurseries tray methods preliminary results, it is clear that, Sida seeds, which were treated with these methods, gave the best growing rate for industrial uses.
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Effect of Foliar Nutrition on Post-Harvest of Onion Seed under Sandy Soil and Saline Irrigation Water Conditions
85-92Views:241Foliar application has been determined to be an effective nutrients delivery strategy in vegetable and fruits. The enhancement of vegetable and fruit yields affected by foliar nutrients application has been recognized in previously conducted studies with perennial tree crops. The efficiency of foliar nutrition is dependent on soil, climate, fertilizer and the amount of nitrogen used. There is no sufficient information concerning cooperation of foliar nutrition with all nutrients form as well as the rates of these nutrients fertilization in vegetable
and fruit crops. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on inflorescences diameter, flower stalk length, number of seed stem /plant, weight of 1000 seed, germination percentage, seed yield, moisture content, catalase , peroxidase activity and malondialdehyde content of onion seeds. The plants
sprayed with union Zn, union Mn, union feer, shams k, elga 600, boron, and amino x had the highest vegetative growth parameter, germination percent and enzyme activity. The plants sprayed with union Zn, union feer, shams K, magnesium, caboron, hummer and amino X had the highest seed yield ha-1. The seeds were stored for one year to study the effect of different commercial compounds and storage temperatures on germination, moisture content and change in antioxidant enzymes activities of onion seeds during the storage period. Storage at cold temperature showed higher germination percent, moisture content and lower malondialdehyde content than storage at room temperature. The treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer and amino x had the highest germination percent. -
Self fertility of pear varieties conditioned by natural self pollination (autogamy)
110-113.Views:287Authors studied the autofertility depending on natural self pollination (autogamy) in 59 pear cultivars during 4 seasons at three locations with different ecological conditions (Helvetia, Kecskemet-Kisfai, Keszthely). The aim of the experiments was to determine the autogamous tendencies of varieties hitherto unexplored in the Hungarien gene bank, or to check data found in the literature. A total of 42616 isolated pear flowers produced 1.2% fruits with at least one viable seed in each. The 59 varieties observed did not set fruit by autogamy on either of the three sites during the four years of the study. The triploid (3n=51) varieties were entirely self-sterile. According to the highest autogamous fruit set, during the experimental period, the varieties have been assigned to four groups: (1) Entirely auto-incompatible (0% fruit set), (2) auto-incompatible (0.1 to 0.9%), slightly self fertile (1.1 to 5%) and (4) self fertile (5.1 to 10%). According to the number of viable seeds per fruit resulting from autogamy, the varieties are assigned to three groups as (1) low seed content (less than 3 seeds per fruit), medium (3.1 to 5) and (3) high (more than 5 seeds). Thus, the assessment of the number of seeds per fruit resulting from autogamy is indispensable as a proof of the absence of parthenocarpy.
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Effects of Foliar Nutrition on Onion Seed Storage under Controlled Atmosphere
101-110.Views:225Storage of fresh fruits and vegetables prolongs their usefulness. The principal goal of storage is to preserve the commodity in its most useable form for the consumer. The extension of storage life and the improvement of quality of fresh fruits and vegetables can be supplied by harvesting at proper maturity, control of post-harvest diseases, chemical treatments, refrigeration, controlled and modified atmospheres. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on onion seeds and storage under controlled atmosphere. The germination percentages were 85.50, 86.85, 83.75 and 82.15 for CA1, CA2, CA3 and CA4, respectively. Water content of onion seeds was significantly increased with decreasing storage temperature in all controlled atmospheres. Water content values were 4.48, 4.40, 4.55 and 4.54 for CA1, CA2, CA3 and CA4, respectively. There were significant decreases in water content with increasing exposure periods. The lowest water content was recordednfor the exposure periods of 120 and 240 days. Catalase and peroxidase activities were decreased with the decreasing of storage temperature, whereas malondialdehyde content was increased. The exposure for 120 days recorded the highest catalase activity. The highest value of peroxidase (11.72) was recorded for CA2. Peroxidase activity increased as exposure period increased until 120 days and then decreased. Cold storage temperature (5°C) showed the highest malondialdehyde content followed by room temperature. The CA2 showed the lowest malondialdehyde content compared with other controlled atmospheres. The exposure period of 120 days had the lowest malondialdehyde content.
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:218The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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Foliar Nutrition and Post-Harvest of Onion Seed: Effects of storage temperatures, storage period and foliar nutrition
29-47.Views:241The aim of onion bulb storage is to meet consumer demand for extended availability of onions whilst maintaining product quality. The principal biological factors leading to onion bulb deterioration are respiration, resumption of growth and pathogen attack. In onion bulbs a dormant period, when sprouting and rooting cannot be induced, is followed by a period of internal changes that prepare the bulb for breaking of dormancy and subsequent growth. Out of storage, the bulb then proceeds towards flowering and seed production. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on yield and yield components of onion seeds and storage The seed yield of each commercial compounds plot from previous experiment was divided into two groups, storage under room temperature and 5°C. Seeds transferred immediately after drying to Increasing Export Competition of Some Vegetable Crops Project Laboratory located in Faculty of Agriculture, Cairo University. The effects of storage temperature, storage period and foliar with some commercial compounds on onion seed quality were considered. Storage in 5°C had the higher germination percentage than storage in room temperature. Results indicated that as storage period increased the germination percent decreased. The treatment with boron or amica in the first season had the highest germination percentage. While, the treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer or amino X had the highest germination percentage in the second season. Storage in 5°C resulted in higher moisture content than storage in room temperature. Regarding the effect of storage period on moisture content, the water content was significantly increased with prolongation of storage period. The lowest values of water content were recorded for treatments with union feer, shams K or boron in the first season, and union feer, shams K, boron, magnesium, shetocare or hummer in the second one. Catalase activity was significantly decreased as storage period increased. The treatment with shams K, boron, shetocare or amino X had the highest catalase activity in both seasons. Peroxidase activity was significantly decreased as storage period increased. Foliar application with boron had the highest peroxidase activity in both seasons. Seed stored in room temperature had the higher malondialdehyde content than those stored in 5°C in the second season. The malondialdehyde content increased as storage period increased. The treatment with magnesium, caboron and the control in the first season, and the treatment with magnesium and the control in the second season had the highest malondialdehyde content.
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Metaxenic pollen effect of scab resistant apple cultivars on the fruit of apple
47-52.Views:164As a part of an apple breeding program, we have examined the pollen effect on the fruit appearance and inner quality of different apple cultivars in the Corvinus University of Budapest Department of Fruit Science. Five apple cultivars ('Golden Reinders', 'Regal Prince', 'Rewena', 'Renora', 'Idared') have been crossed by the pollen of some new and some traditional cultivars in our experiment. The measurements were carried out in the analytic and fruit examining laboratory of the Department of Fruit Science. The diameter, height and stem length were measured by digital caliper. We have also examined the squash ratio, soluble solid- and acidic content. Sugar content was calculated by a schedule released by the International Sugar Committee (ISC). From the sugar- and acidic content, we could also count the Pomona-value.
We have observed interesting effects according to some attributes in some crossing combinations with the help of statistical analysis. Crossing 'Golden Delicious' with 'Freedom', 'Prima', and 'Baujade' have resulted in smaller fruit size than other combinations. No significant differences could be observed on `Golden Reinders' fruits according to metaxenic coloration in the year of the examination. On the other hand the color modifying effect of pollen donating cultivars have manifested on the red fruits of 'Regal Prince' (Gala Must). `Freedom' as a pollen donating cultivar has resulted outstanding value in our consistency measurements. Squash ratio was the highest in combinations pollinated by 'Prima' and `Reglindis'. We have noticed balanced values when measuring inner qualities, only `Reka' has caused lower acidic content. Significant differences have been found in combinations according to the number of frivolous seeds. No frivolous seeds were found in the combinations 'Golden Reinders' x 'Freedom' and 'Idared' x 'Prima'. Almost two frivolous seeds were found in the fruits of the combination 'Renora' x 'Sóvári' in average.
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Time of flowering and fertilisation of quince varieties
9-15.Views:290Literature dealing with flowering and fertilisation of quince is scarce. Most controversial and scanty are informations on observations of self- and cross-pollination. According to our observations, differences in blooming time are few (2-3) days only, thus flowering of most varieties is synchronous. The varieties observed are grouped as early, intermediate and late flowering ones. Self fertility of the individual varieties, however, was not assessed unequivocally, therefore it is recommended, by safety reasons, to consider quince actually as a whole to be auto-incompatible. Artificial self-pollination (or rather geitonogamy) as well as cross pollination with other varieties increased substantially fruit set if compared with the results of natural self-pollination (autogamy). According to the fruit set of their open pollinated flowers, varieties have been classified according to fertility as low (below 10 %), medium (between 10 and 20 %) and high (more than 20 %). Cross fertility of varieties is highly variable depending on combination and on season. Contradictory data are probably due to the sensitivity of quince to conditions of search. Better fruit set was coincident with higher number of stout seeds per fruit. Well developed seeds are definitely a prerequisite of larger fruit size.
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Effect of sowing dates and NPK levels on active substances in seeds of isabgol plants (Plantago ovata Forsk, L.).
89-92.Views:132Two field experiments were carried out in the Experimental Station and Laboratory of the Vegetables and Floriculture Dept., Fac. of Agric., Mansoura Univ., during the winter seasons of 1995/96 and 1996/97. The investigation aimed to study the effect of different sowing dates and NPK fertilization levels, as well as their interactions on active substances extracted from Isabgol plant (mucilage contents). Four sowing dates and six NPK levels were studied. The main findings in this investigation can be summarized as follows:
- Plants grown in the first sowing data (Nov. 1st week) produced the highest content of mucilage as g/5 g seeds or as percentage of mucilage per plant.
- The addition of NPK fertilization significantly increased the mucilage content of seed and its percentage per plant. The highest content and percentage were in plants treated with the fourth level of NPK (178.0 g/3m2).
- Concerning effect of interaction between sowing dates and NPK levels on mucilage content, the highest content and percentage per plant were produced in plants grown in the first sowing date and treated with the fourth level of NPK.
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Assessment of different rates of NPK fertilizer on the growth and yield components of two exotic okra (Abelmoschus esculentus L.) western urban of Sierra Leone
55-59.Views:353The experiment was conducted in an Inland Valley Swamp during the dry spell of 2017 laid out in a randomized complete block design (RCBD) three replications at the Kabala Horticultural Crops Research Centre (KHCRC) cropping site, Ogoo Farm of the Sierra Leone Agricultural Research Institute (SLARI), Freetown, Sierra Leone. Raised beds measuring 5m x 1m (6 m2) with a 1m furrow between beds. Inter and intra row spacing of 75 cm by 50 cm with two rows per bed and two seeds per hills were directly sown. The treatments constituted NPK 15:15:15 at the rate of 0, 90, 120 and 150 kg/ha designated as T1, T2, T3 & T4 and Two hybrids of okra were used as experimental materials obtained from Indo-American Hybrid Seed Company, India; Okra-WASA-HyOKR-1 and Okra-WASA-HyOKR-2. Seeds were treated with Thiram Poison for longevity. Result indicates T2 (25.2; 35.1 cm) and T4 (56.9 cm) treatments recorded tallest plant heights followed by T2 (24.0, 34.4 & 50.1 cm), while T1 (18.8, 28.4 & 33.3 cm) had the shortest plant heights at 4, 8 & 10 Week After Planting respectively. Similarly, T4 & T2 (38.9, 65.5 & 44.8 cm) exhibited the largest SG and T1 (21.7, 33.0 & 52.0 cm) the least values at 4, 8 & 10 Week After Planting. While, the widest and narrowest LA at 4, 8 & 10 Week After Planting was observed in T3 (131.3, 135.0 & 158.5 cm2) and T1 (120.8, 121.8 & 128.1 cm2). With respect to yield and yield components, the longest (22.0 cm) and heaviest (112.7 g) fruit were recorded by T3 followed by T2 (19.1 cm & 101.3 g) with T1 having the shortest (11.7 cm) and lightest (52.3 g). The width of pod in T3 (31.2 cm) was greater than the rest of treatments and as usual, T1 indicated the least value (14.6 cm) respectively. The average NP produced plant-1 was shown by T2 & T3 (4.0) with the least of fruit produced by T1 (2.0). Also, 100 seed weight, T3 (106.7 g) weighted the highest followed by T2 (103.3 g), while T1 (60.3 g) accounted for the lowest seed weight. Conclusively, the two materials are adaptable to the climatic conditions of Sierra Leone (Western area). Hence further evaluation required across vegetable growing zones.
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Investigation of some production factors on yield and quality of dry beans
83-88.Views:139The correlation between plant density and yield average shows that the volume of yield increases in varieties of large and medium-size seeds up to a plant density of 285-400 thousand/ha, after which it declines.
On the basis of the results, yield averages at plant densities of 285-334 thousand plant/ha are 0.17 t/ha higher than those achieved at low density (200 thousand plant/ha).
High levels of potassium fertilizer did not enhance the yield of dry beans. With adequate-water supply the high level of potassium decreased the number of pods and seeds per plant compared with a basic level of fertilizer, which could ensure 2.5 t/ha yield.
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Effect of intensity of bee visitation and the foraging behaviour of honeybees on the fruit set and yield of apple (Malus domestica Borkh.)
31-39.Views:155Based on the results of our experiments, both the relative and the effective intensity of bee visitation were rather different depending on the cultivars as well as the time of the day. While it varied between relatively extreme values (40-80%) in the morning for the varieties examined, there were much smaller differences between the intensity of bee visitation at the afternoon, because the relative bee visitation attained 70-90% at each cultivar. These results showed that the differences arising from intensity of bee visitation of different cultivars should be taken into consideration more carefully in the morning in orchard planning and in estimating the number of honeybee colonies required.
The results showed that the greatest percentage of fruit set and the highest number of viable seeds per fruit were measured on branches of those cultivars that were most frequently visited by pure pollen gatherer bees as well as by bees collecting both nectar and pollen (mixed behaviour). The effect of pure pollen gatherers and of bees with mixed behaviour was highly significant from the statistical point of view on the fruit set and the number of viable seeds per fruit.
Those bees that were sucking nectar only from apple flowers did not proved to be effective pollinators at all. Relationship between their number and the fruit set as well as the number of viable seed per fruit were not significant because the coefficient of correlation was close to nil.
The ratio of side worker nectar gatherers was negatively correlated with the fruit set and the seed content of fruits of apple cultivars examined at both of our experimental sites, at Mosonmagyaróvár and Feketeerdő as well. The presence of side worker nectar gatherers resulted in higher decrease of fruit set and seed content of fruits at Feketeerd6 than at Mosonmagyaróvár, especially in the morning.
The effect of flower visiting intensity by other pollinating insects was found to be fairly variable according to the time of the day. In the morning they had no effect on the fruit set as well as on the seed content of fruits either at Mosonmagyaróvár or at Feketeerdo. However, at the afternoon, when their intensity was greater, the correlation was a bit stronger.
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Studies on some seed traits of Iris pumila L., Adonis vernalis L., Primula veris Huds. and Alkanna tinctoria (L.) Tausch.
36-40.Views:138In this study we summarize the results of a five-years period concerning seed traits examinations on Iris pumila, Adonis vernalis, Primula veris and Alkanna tinctoria, with special attention to seed dimensions, seed mass and other traits concerning plant fitness, to their variability and the relationship among them. We found tight correlation between seed weight and seed dimensions in Adonis and Primula, at the same time no correlation exists among the same characters in Alkanna tinctoria. Consequently, the seed weight and seed dimensions can be used as synonyms in the form of „seed size" only after preliminary detection of correlations among them.
The variability of seed traits is higher in natural categories (individuals, morphs) than in seed mass categories as speculative groups. When we need homogeneous plant stand (e.g. for an introduction experiment) it is suggested to use seeds pre-selected in this way. For ex situ conservation, where the central goal is to maintain the genetic variability, seeds originated from different individuals are preferred.
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Optimization of sample preparation for determination of antioxidant parameters from one grape berry
93-97.Views:222Berry quality is an important issue in wineprocessing, however evaluating characteristics in a single berry level is very complex. The Phenolic compound analysis is more challenging because of the limited amount of material and a mixture of skin, pulp and seed. No clear protocol exists for evaluating these compounds from single berries. The aim of our investigation was to develop such a protocol. Single whole grape berries samples were homogenized in 20 replicates by 5 different methods. The most effective method was when berry was placed in a mortar and was crushed with a pestle under liquid nitrogen until seeds were clearly visible. Seeds were then taken from the mortar and crushed between two stainless steel plates with a hammer, and returned to the mortar without residues. Homogenization continued until the sample had a powdery appearance. The homogenized samples were taken for further analysis such as total polyphenols, total anthocyanin contents and total antioxidant capacity. Our results demonstrate that single berries could be processed and that the homogenates were suitable for taking chemically uniform subsamples.
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Luminescence variations in cucumber (Cucumis sativus L.) leaves derived from different regeneration systems
50-52.Views:164Plants obtained from in vitro culture can show increased susceptibility to environmental stress conditions. In the process of their adaptation to natural conditions it requires monitoring of their physiological state. The methods used to check this phenomenon should estimate quickly and exactly the tolerance to suboptimal environmental factors. Such requirements are satisfied by the methods of measuring chlorophyll luminescence in vivo, e.g. fluorescence induction and delayed luminescence. The objects of our studies were cucumber plants regenerated from cultures of callus and embryogenic cell suspension, as well as the plants obtained from seeds. The plants derived from in vitro cultures displayed a poor physiological condition at the early phase of adaptation characterised by higher susceptibility both to stress caused by increased density of the light flux and low temperature (4 °C) in comparison with the plants obtained from seeds.
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Propagation from root cuttings for black locust (Robinia pseudoacacia L.) improvement in Hungary: a review
39-41.Views:326Black locust (Robinia pseudoacacia L.) is a valuable stand-forming tree species introduced to Europe approximately 400 years ago from North America. Today it is widely planted throughout the world, first of all for wood production. In Hungary, where black locust has great importance in the forest management, it is mainly propagated by seeds. But since the seed-raised plants present a great genetic variation, this type of propagation can not be used for Robinia’s improved cultivars. In the Hungarian black locust clonal forestry, propagation from root cuttings can be used for reproduction of superior individuals or cultivars in large quantities. However, this method demands more care than raising seedlings from seeds and can be applied with success in well-equipped nurseries.
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Efficacy of ethrel (2-chloroethyl phosphonic acid) as a chemical hybridizing agent in red pepper (Capsicum annuum L. var. Pusa jwala)
41-44Views:181A field experiment was conducted during 2003-2004 and 2004-2005 to study the effect of foliar sprays of ethrel or ethephon (2- chloroethyl phosphonic acid) on pollen sterility and yield parameters in Capsicum annuum var. Pusa jwala. Effect of treatments was also studied in F1 hybrids raised from treated male sterile plants crossed with the control plants. Plants sprayed with 0.1, 0.2 and 0.3% (v/v) ethrel exhibited 93.1-100% pollen sterility. This was associated with significant reduction in yield parameters (number of flowers, fruits/plant, fruit size, number of seeds/fruit and total yield/plant). However, the plants sprayed only once with 0.1% ethrel at pre-meiotic stage showed 93.1% pollen sterility without any significant reduction in yield parameter. The F1 hybrids obtained by crossing the 100% male sterile treated plants with the pollen of untreated (control) plants exhibited only insignificant reduction in the number of flowers/ plant, fruits/plant, fruit size, number of seeds/fruit and total yield/plant. However, these parameters in F1 hybrids were significantly higher over the treated plants.
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Severely pollen-limited fruit set in a pear (Pyrus communis) orchard revealed by yield assessments and DNA-based paternity assignment of seedlings
67-74.Views:186In commercial fruit tree orchards, consistently high yields are necessary for a durable economy. The Swedish pear cultivar 'Carola' has been noted for low setting in some orchards, possibly due to insufficient pollination. In this study, fruit set was evaluated in a research orchard where `Carola' had been planted together with four potential pollinators. Total yield and number of fruits was noted during three and four years, respectively. In 2003, seeds were germinated from the harvested `Carola' fruits, and the paternity of three seedlings from 50 trees was determined with RAPD analysis. 'Clapp's Favourite' had sired 39.6% of the seedlings, closely followed by `Seigneur d'Esperen' (30.7%) and 'Clara Frijs' (26.7%) whereas 'Skanskt sockerparon' only sired 1.1% of the seedlings. The remaining 2.3% appeared to have been derived by selfing. Pollen-limited seed set was indicated at surprisingly short distances; accumulated number of fruits on the `Carola' trees was significantly higher when separated by only 2 m from one of the two most efficient pollinators, 'Clapp's Favourite' or 'Seigneur d'Esperen‘, compared to trees 4—l0 in away in the same row. Number of viable seeds per fruit was also higher in fruits from trees immediately adjacent to the pollinators, suggesting an effect of improved pollination success. The importance of very short inter-cultivar distances for efficient pollen transfer became even more clear when comparisons involved the true pollination distances as determined by RAPD; the accumulated yields decreased linearly from 55 kg at a 2 in distance to only 17 kg at 13 m.