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Leaf number and plant height of three Canna x generalis cultivars
22-24.Views:258The aim of this three-year study was to evaluate the effect of three treatments (thiophanate methyl, charcoal dust, and control) on leaf number and plant height on three Canna x generalis cultivars (City of Portland, Richard Wallace and Robert Kemp). Results showed that the 3-year means of leaf number ranged from 13.9 to 23.1 for the three cultivars. The largest leaf numbers were for cultivar Robert Camp (23.1 in 2010) and the lowest for cultivar City of Portland (13.9 in 2011). Thiophanate methyl treatment produced the highest number of leaves and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Numbers of leaves in charcoal dust treatments were larger than the control treatments but it was not significantly different. Results on plant height ranged from 69.2 to 160.5 cm for the three cultivars. The largest plant height was for cultivar Robert Kemp (160.5 cm in 2011) and the lowest for cultivar City of Portland (69.2 cm in 2011). Thiophanate methyl treatment produced the highest plant height and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Plant heights in charcoal dust treatments were larger than the control treatments but it was not significantly different.
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Characterization of sunburn damage to apple fruits and leaves
15-20.Views:674The specific conditions of the formation of three different types of sunburn (sunburn browning, sunburn necrosis, and photooxidative sunburn) have been recently characterized on apple fruit. However, no information is still available on leaf damage. Therefore, the aims of this study were i) to extend the knowledge on fruit damage, ii) characterize leaf damage and iii) find relationship between fruit and leaf damage. The observations were made on 586 apple accessions in a gene bank orchard located in Hungary. The incidence of the three different types of fruit symptoms were recorded and based on the visual symptoms, two different types of leaf sunburn (sunburn yellowing and sunburn necrosis) were characterized. The most frequent type of fruit sunburn observed was sunburn browning. Photooxidative sunburn was found for less number of accessions, and only some accessions were affected by sunburn necrosis. Fruit were far more susceptible than leaves; (>60%) of the examined accessions were affected by fruit damage and (<3%) by leaf damage. Although a large number of accessions were affected, the percentage of fruit damaged within accessions was not that excessive; ~6% of the fruit assessed showed the symptoms of sunburn browning. Significantly fewer fruit were damaged by sunburn necrosis (~1%) or photooxidative sunburn (~1.4%) than sunburn browning. The percentage of leaves damaged within accessions were simlarly very low (~1%). Close relationship between fruit and leaf damage was found. Accesions with relatively heavily sunburned leaves usually had severe fruit damage as well. Leaves showing sunburn symptoms were usually closely located around those fruit which were sunburned severely. Leaf damage of sunburn was found on spur leaves in a great majority of the accessions damaged, shoot leaves did not seem to be susceptible to sunburn.
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The impacts of different habitats on the development of Telekia speiosa (Schreb.) Baumg.
31-32.Views:181Telekia speiosa (Schreb.) Baumg. is a 100-150 cm high bushy perennial, which has yellow flowers and smells good. According to the descriptions (Farkas, 1999), it can be detected in two smaller areas within Hungary, namely in the Bükk hills and on the Szatmár-Bereg Plain. By the time of writing this paper, the population around Tiszabecs has already got extinct. Therefore, it is a protected relict species. It is named in honour of Sámuel Teleki, chancellor of Transylvania.Within the frame of the experiment, the Telekia speciosa (Schreb.) Baumg. was planted to places differently illuminated (sunny, semi shadow, shade), then the morphological changes brought about the various light conditions were investigated. The experiment was launched with a stock sown in October 2008. The seedlings were planted to three beds with diverse light conditions. The area of each bed was 1 m2, and ten seedlings were planted per m2. The parameters investigated are as follows: the length of leaf blade, the width of leaf blade, the length of petiole, the number of leaves per plant, and the alterations of leaves. As a result of our research, we can state that semi shadow is the optimal habitat for the plant. Under such ecological conditions the highest leaf production was observed, the leaves were species specific, healthy and big. The mean number of leaves per plant was 6.6, the mean length of blade was 16.6 cm, the mean width of blade was 13 cm, while the mean length of petiole was 14.2 cm. In the shade the plants grew poorly and the size of leaves were smaller. The mean number of leaves per plant was 4.1, the mean length of blade was 8.6 cm, the mean width of blade was 7.1 cm, and the mean length of petiole was 9.4 cm. In the sunny habitat a similarly high leaf production was observed as in the semi shadow; however, the leaves had brownish spots, they shriveled, feel rough, so they revealed a reduced aesthetical value. The mean number of leaves per plant was 6.6, the mean length of blade was 17.8 cm, the mean width of blade was 11.3 cm, and the mean length of petiole was 13.1 cm.
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The effect of the method and the timing of the propagation on the growth, earliness and productivity of sweet corn
134-139.Views:188Direct sowing in 16 cm deep trench covered with perforated plastic sheet (for 3 weeks), transplant using, and uncovered direct sowing (control) was tried on 2 locations, with 2 varieties (very early Kecskeméti korai extra, and middle early Kecskeméti SC-370) in Szarvas on loamy soil, and in Kecskemét on sandy soil in 1996 — after a preliminary trial concerning perforated plastic covered trench sowing in Szarvas, in 1995.
The plant height (weekly), the average leaf number/plant, the total leaf area (once), the total yield, the quality of cobs, and the earliness was measured. The results are:
- Kecskeméti korai extra during the first 6 week period the transplanted plants were the highest, but from the 7th week the plants which were sown in trench and than were covered with perforated plastic sheet (for 3 weeks) were the highest.
- SC-370: The transplanted plants were the highest-until the end of plant height development.
The plant height development stopped at the 9th week of the measurement by early, - and stopped at the 10th week by middle early variety. The average leaf number/plant varied between 9,25-10,50 and was not influenced either by variety or by the treatment. The total leaf area was (on 5th of June) the largest by transplanted plants, which was followed by plants that were sown in trench and then were covered with perforated plastic film (for 3 weeks).
- The highest yield was observed by plants, which Were sown in trench, and then were covered with perforated plastic film (for 3 weeks). Transplanted plants followed it.
- Quite the total yield (98,3%) of transplanted Kecskeméti korai extra variety plants were harvested on 4th July. 89% of the total yield was picked up of trench sown and then with perforated plastic covered plants. The harvest of uncovered control started on 15th July. The harvest of transplanted SC-370 plants started 19th July, when more than half of the total yield (57%) was picked. The uncovered control was harvested 29th July.
- The weight and the measure of cobs generally were not influenced by the treatments, but the average weight of the cobs of the transplanted Kecskeméti korai extra plants (0,21-0,18 kg) are less than the, requirement.
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Micro area based spatial distribution of apple scab in an organic apple orchard
21-23.Views:269In this study, the objective was to report a two-year investigation on micro area based spatial distribution of apple scab in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 587 and 623 on leaf and between 46 and 78 on fruit for an individual tree. Number of asymptomatic plant part ranged between 1034 and 1321 on leaf and between 119 and 193 on fruit. Disease incidence ranged between 35 and 40% on leaf and between 27 and 33% on fruit. Disease aggregation index ranged between 0.115 and 0.298 on leaf and between 0.117 and 0.221 on fruit. Three of the four trees showed significant within canopy aggregation of disease for leaf apple scab symptoms in both years. For fruit apple scab, two of the four trees showed significant random patterns in both years.
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Selection of the chance seedlings of `Mézes körte' (Pyrus communis L.) from the gene bank of Keszthely
21-27.Views:259We have concluded the selection tests of the `Mézes körte' seedlings planted in the spring of 2006, with special emphasis on the cotyledonary, foliage leaf and the height of plant. Out of the 75 seeds planted in rows, there were 40-45 pieces growing out, so during the first cotyledonary test we had to calculate with almost 40% decay. On 12th April 2006, we recorded some of the important characteristics of the seedlings in their cotyledonary stage which characteristics were important from the point of view of selection (cotyledonary form, cotyledonary length, cotyledonary thickness, cotyledonary colour, cotyledonary petiole length, cotyledonary petiole thickness, cotyledonary petiole colour). The above morphological characteristics are shown in Table No. 1-6. We have also tested the seedling in foliage leaf state, paying special attention on the development stage of the plants (colour of foliage leaf , height of plant). We have completed statistical calculations of the two above mentioned characteristics. The result of that is summarised in Table No. 8-9. The variation coefficient show smaller value in the case of the foliage leaf number (15-32%), while the wider range of spread of the data referring to the height of the plant is shown by the 33-61% CV values. On charts No 4-9. we present the relationship between the height of the plant and the number of foliage leaf, as well as the differences between the two graphs. Based on the above charts and graphs it can be defined that the 40% destruction of the developing seedlings during the period till the next measurement reached 70-80% level. In spite of this however some seedlings showed strong and balanced growth (A44, B42, C25, D16, E5, E39, F38), the further testing and selection of those is to be completed in the future.
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Effect of straw and plastic mulches on growth and yield of zero tillage potato (Solanum tuberosum L.) in the Coastal Khulna Region of Bangladesh
23-30.Views:64A randomized complete block design experiment was undertaken at the field laboratory of the Agrotechnology Discipline, Khulna University, Khulna, from November 2021 to March 2022 to ascertain the impact of various mulch treatments on zero tillage potato cultivation. The experiment employed BARI Alu 78 as the planting material and utilized three replications with five distinct mulch treatments. These mulch treatments were identified as follows: Straw mulch (T1), Mulch film (T2), Black polythene (T3), White polythene (T4), and Blue polythene (T5). The metrics that were recorded included the number of sprouts, plant height, number of leaves per plant, leaf width, leaf length, number of tubers, tuber length, tuber breadth, number of defective tubers, weight of defective tubers, and tuber weight. Different mulching techniques significantly influenced the overall crop yield and the underlying factors that influenced the results. The mulch film treatment resulted in the highest yield, with a production of 34.76 tons per hectare, followed by black polythene (28.11 tons per hectare), straw mulch (23.74 tons per hectare), white polythene (20.44 tons per hectare), and blue polythene (18.66 tons per hectare). The economic analysis revealed that mulch film had a high benefit cost ratio along with high input and output. Black polythene came in second place when the benefit cost ratio was compared to that of mulch film and the rest of the three treatments. Combining zero tillage with mulch film or black polythene demonstrates significant potential for becoming a cost-effective practice in the coastal region of Bangladesh.
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Rootstock effects on fruit drop and quality of 'Arlet' apples
69-75.Views:321The aim of this study is to investigate the effect of different growth inducing rootstocks on fruit drop of apple. This research was conducted for 3 years at Nagykutas, Western Hungary on apple cv. 'Arlet'. For the experiments, 3 different growth inducing rootstocks were choosen: M.9 (weak), MM.106 (moderate) and crabapple seedling (strong). There were 3 fruit shedding periods on the trees grafted M.9 and MM.106 rootstocks, but in the case of crabapple seedling, only 2 were found. The measure of fruit drop was closely related to seed count of fruits; seed number was the lowest, fruit drop was the highest. The lowest seed number was counted in fruits from trees on crabapple seedling. Seasonal changes of leaf:fruit ratio mainly depended on shoot growth and fruit drop. The rise of the curve of leaf:fruit ratio was very important during the first phase of fruit development, in especially at the end of June and in the beginning of July. The cause of this is that first and second periods of fruit drop appeared during this term. The rise of the curve was important in the beginning of June and the end of July on crabapple seedling. Decreasing tendency of quality parameters was found of fruits from trees on M.9, MM.106 and seedling rootstocks, except flesh firmness which was the highest in fruits from trees on crabapple seedling.
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Evaluation of fruit tree waterstress condition and transpiration with heat fl ux sensors
leaf area index, evapotranspiration, water stressViews:253In this study the transpiration properties and response to heat stress of Granny Smith and Jonagold apple species were measured. The examinations were set at an orchard near to Debrecen. The chosen trees were two years old, without fruits, in 20l container, placed in the mixture of mulch matrix, wood chips and sawdust and grown at the same climatic conditions. One of the most important goals was to determine the leaf area of the trees which is a basic data for transpiration modeling. Leaf area was larger (signifi cance level p<0.05) in the upper part of the canopy to achieve larger photosynthetic surface. Jonagold species with less leaf number (–18% compared to Granny Smith) reached almost the same canopy area, than Granny Smith, due to larger leaf area of Jonagold. Another aim was to measure the transpiration rate of the apple trees. The transpiration measurements were carried out by sap fl ow meter. Only a small transpiration difference can be found between species. During the investigation time there were wet and cool as well as dry and extremely hot periods. In the case of dry and extreme hot periods the transpiration is decreased, which causes decrease in transpiration and enhanced the water stress of trees.
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Rhizogenesis in in vitro shoot cultures of passion fruit (Passiflora edulis f. flavicarpa Deg.) is affected by ethylene precursor and by inhibitors
47-54.Views:180The effects of the ethylene precursor ACC and two inhibitors, AgNO3 and AVG, on root formation were tested in in vitro shoots of passion fruit (Passiflora Midis f.flavicalpa Deg.). The organogenic response was assessed on the basis of percentage of shoot-forming. roots, root number and length. The time course of ethylene production was also monitored. ACC inhibited root formation by delaying root emergence and increasine, callus formation at the basis of the shoots. In addition, ACC caused a marked increase in ethylene production, coupled to leaf chlorosis and senescence with lower rooting frequencies, number and length of roots. IAA supplementation increased ethylene production. Both ethylene inhibitors, AgNO3 and AVG, at appropriate concentrations reduced callus formation at the basis of shoots. AVG increased the number of roots per shoot, but drastically reduced length of differentiated roots. Regarding to leaf pigments, ACC promoted a marked reduction on carotenoids and total chlorophyll, whereas AVG and AgNO3 delayed explant senescence and pigments degradation, not differing from IAA supplemented and non-supplemented control treatments. The results confirm previous reports on the beneficial effects of ethylene inhibitors on in vitro rooting and suggest its reliability to be used as an alternative approach to evaluate sensitivity of Passiflora species to ethylene.
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Long term investigations of flowers and leaves on mainly non-domestica plums
73-79.Views:190The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings of C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification.
The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data.
Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).
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The effect of Ferbanat L nano-fertilizer on the growing of Petunia x grandiflora ’Musica Blue’
107-109.Views:280During our experiment, the effect of Ferbanat L concentrations were examined by Petunia x grandiflora ‘Musica Blue’ production. The leaf and shoot length, number of flower buds, diameter of flowers, and the date of appearing the first buds were measured. The solution of Ferbanat L in 0.1 % concentration was the most effective on shoot length (21.7 cm). Remarkable increase was observed by the other treatment groups as well comparing to the control group (5.6 cm), the plants treated with solution of Ferbanat L 0.2% (16.5 cm) and 0.3% (14.4 cm) had significant effect as well. The nano-fertilizer had not positive effect on the other examined parameters as leaf and flower size, number of flower buds or chlorophyll content. The culture period shortened five days.
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The effect of 5-aminolevulinic-acid (ALA) on the development of Saintpaulia ionantha
33-36.Views:302In recent work the effect of 5-aminolevulinic-acid (ALA) agent (commercial name Pentakeep-V) was examined on the chlorophyll content, growth and development of Saintpaulia ionantha. The newly re-rooted potted plants were irrigated or sprayed with 0.3‰ or 0.5‰ Pentakeep-V solution, and plus 30% long lasting fertilizer was added to half of the all treatments. Control plants were sprayed with tap water. Best result were obtained on the field of flowering. All the treatments promoted chlorophyll-content in the leaves especially spraying with 0.3‰concentration. Plants treated with Pentakeep-V in both concentrations and independently from the spraying or irrigation flowered more than two weeks earlier than control and those that got plus 30% fertilizer. Besides in the case of some treatments the diameter of leaf rosette, the number and surface size of leaves grew comparing to the control. The longlasting fertilizer had positive effect on the fresh weight but none of treatments had effect on the dry weight.
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The tissue structure of the vegetative organs of strawberry (Fragaria moschata Duch®)
28-31.Views:233The tissue structure of the vegetative organs of strawberry (root, rhizome, stolon, leaf) is discussed in this paper. The authors stated that the root structure described by Muromcev (1969) and Naumann-Seip (1989) develops further from the primary structure. It grows secondarily and the transport tissue becomes continuous having ring shape. In the primary cortex of the rhizome periderm like tissue differentiates, but according to the examinations up to now, it does not take over the role of the exodermis. The exodermis is phloboran filled primary cortex tissue with 3-4 cell rows under the rhizodermis. The development of the transport tissue of the petiole is also a new recognition. In the lower third of the petiole the transport tissue consists of 3 collaterally compound vascular bundles. In the middle third there are 5 bundles because of the separation of the central bundle and in the upper third of the petiole 7 bundles can be observed because of the ramification of the outside bundles. Therefore attention must be taken also in the case of other plants at making sections. There might be confusions in the results of the examinations if the number of bundles increases in the petiole. The tissue structure might vary depending on the origin of the tissue segment.
The palisade parenchyma of the leaf blade has two layers and it is wider than the spongy parenchyma. Among the 5-6-angular cells of the upper epidermis do not develop stomata while in the lower epidermis there are a fairly lot of them.
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Water consumption of the wine grape varieties Kövidinka K.8 and White Riesling B.7
25-30.Views:147In the Carpathian Basin Kövidinka and White Riesling are promising wine grape varieties. As in the region continental climate dominates and dry years are not uncommon it was natural to study the water requirement and consumption of the two varieties. Morphological characters affecting transpiration were observed including leaf area, hairiness, number and type of stomata. The amount of water transpired per unit leaf area and time and rate of water consumption were measured in a model trial in cuttings with known water supply.
The water consumption of vine cuttings depends on varieties and is determined by the genotype but it is also affected by environment. Kövidinka requires little water and uses it to its advantage White Riesling requires more water and uses it rather lavishly. The results of our model trial could be introduced directly into viticulture practice.
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Effects of aromatic cytokinins on structural characteristics of leaves and their post- effects on subsequent shoot regeneration from in vitro apple leaves of 'Royal Gala'
41-46.Views:185The effects of different aromatic cytokinins applied in different concentrations and combinations were investigated on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves were studied. Great differences in the anatomical structure of leaves could be detected originating from media containing different types and concentrations of aromatic cytokinins. The number of regenerated shoots per explant and the organogenetic index were used for the evaluation of the post-effect of aromatic cytokinins on shoot regeneration. The histological structure of leaves used for regeneration and their regeneration response showed a good correlation. When the pre-treatment caused a juvenile-like or less-differentiated structure, the number of regenerated shoots per explant increased and often vitrification also decreased and consequently the organogenetic index also increased. A strong interaction between cytokinin-content (type and concentration) of the pre-treatment medium and that of the regeneration medium could also be detected.
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Preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard
35-37.Views:217In this study, the objective was to report a preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 11 and 20 on shoot and between 9 and 24 on fruit. Number of asymptomatic plant part ranged between 85 and 109 on shoot and between 133 and 206 on fruit. Disease incidence ranged between 13.8 and 17.6% on shoot and between 9.1 and 11.3% on fruit. Disease aggregation index ranged between 0.098 and 0.228 on shoot and between 0.043 and 0.108 on fruit. One of the four trees showed significant within canopy aggregation of disease for shoot powdery mildew symptoms in both years. For leaf powdery mildew, all tree exhibited random patterns in both years.
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Bud-, flower- and fruit-density in stone fruits
59-69.Views:343In 164 varieties of five stone fruit species, counts of flower buds, flowers and fruits set have been performed, regularly, between 1982 and 2002. The critical number and sample size has been determined for the purpose to estimate the yielding potential of peach plantations. For a rapid test, 10 shoots per variety are recommended. In sour cherry and peach varieties, the number and ratio of leaf and flower buds has been assessed on bearing shoots of different length.
The typical flower bud density of 129 peach varieties varies, as a rule, between 0.13 and 1.10 bud/cm. Three groups of flower-bud-densities could be distinguished: low (0-0.40 bud/cm), intermediate (0.41-0.60 bud/cm), high (more than 0.60 bud/cm). About 62% of varieties belong to the intermediate group. Negative correlation has been found between flower density and relative fruit set, whereas positive correlation between flower density and fruit yield.
The results are utilised in the description and choice of varieties, moreover, in choosing of optimal pruning policies. Varieties of high flower bud densities are recommended to be preferred for growing sites with frequent late frosts. Abundantly yielding varieties of low vegetative vigour are to be pruned more severely than those characterised by low yields, vigorous growth and low flower density. Sour cherry varieties, which are inclined to grow "whips" ought to be stimulated to grow longer shoots (40-50 cm per year), than varieties woid of that tendency (30-40 cm).
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Effects of different cytokinins on the shoot regeneration from apple leaves of 'Royal Gala' and 'M.26'
69-75.Views:220The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron, or 6-benzylaminopurine, or meta-topolin, or zeatin, or kinetin, or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg 1-1. Effects of 'these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN). Organogenetic index (0I) calculated from these data was used for the evaluation of efficacy of cytokinins. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, 01: 7.5) was achieved in `Royal Gala' by using TDZ in concentration of 0.5 mg 1-1 (2.271,1M). There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins, namely N9-ribosides applied in less concentration than nonribosides have the same or best regeneration effects except for 6-benzylaminopurine riboside. However, similar relationship could not be detected in the case of 'M.26'. SN was the highest (3.22) using 6.5 mg 1-1 (18.2011M) 6-benzylaminopurine riboside or 8.0 mg 1-1 (21.44 µM) meta-topolin riboside (3.18). SN was not significantly lower (3.12) by using 2.0 mg 1-1 (9.08 1M) TDZ, however, OI was about half as big (0.63 compared to 1.29 or 1.74 with 6-benzylaminopurine riboside or meta-topolin riboside, respectively). 'Royal Gala' had higher organogenetic ability, than `M.26': 3.5-fold higher shoot number per explant and more than 4-fold higher organogenetic index was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26' and if explants of 'Royal Gala' were further cultured with 3 weeks, SN increased from 11.08 to 24.42 on TDZ-containing regeneration medium, which might suggest higher organogenetic ability, too.
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Bioactive phenols in leaves of Forsythia species
57-59.Views:240Nowadays a number of lignans (arctigenin, matairesinol, pinoresinol and phillygenin) have come to the fore in research due to their various biological activities. In this paper the accumulation of these constituents in leaf extracts of Forsythia plants (F. intermedia, F. ovata 'Robusta’ and `Tetragold', F. suspensa, F. viridissima) was quantified using a new isolation method, supercritical CO2 fluid extraction. The total phenolic and flavonoid contents, the antioxidant capacity and the aglycone lignan profile were determined in leaf extracts of Forsythia species. Within the phenols, the flavonoids were only present in small quantities, but the amount of aglycone lignans was extremely high. F. ovata `Robusta' had the highest total lignan content (103.8 mg/g) of all the Forsythia species. The main lignan in this species is arctigenin, which normally makes up about 60% of the total lignan content, but in the case of F. ovata `Robusta' this value was 96.1%. Since this arctigenin content is outstanding compared to that of other Forsythia species, it could be promising to develop a fermentation technology for the production of this natural compound.
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Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia
46-52.Views:149The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha-1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.
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The effect of modified bacterial virulence to host-pathogen relationship (Phaseolus vulgaris L. Pseudomonas savastanoi pv. phaseolicola)
53-56.Views:221The Pseudomonas savastanoi pv. phaseolicola is one of the most expressive biogen stressors of the bean (Phaseolus vulgaris L.) in Hungary. The chemical and agrotechnological defence is inefficient, so breeding is the only workable way. The conventional cultivars are susceptible to PS while most of the new industrial varieties have genetic resistance to the pathogen. The genetic background of resistance is, however, a complex system in the bean. Leaf resistance is a monogenic system, but this gene is not expressed in juvenile stage of the host. The pathogen species can be divided into different races. After inoculation with virulent strains, typical symptoms appeared on the leaves. To understand the details of host-pathogen relationships, there were carried out experiments using bacterial strains with altered virulence. Six transposon mutants of the PS were tested. Our main objective was to test these modified bacterial strains on bean cultivars of known genetic background. First we analysed the symptoms, and then the correlation between the symptoms and the multiplication of mutant bacteria. Three cultivars (Cherokee, Inka and Főnix) were tested.
The infection by the virulent PS isolate produced typical symptoms on the three cultivars tested. Mutant bacteria (except strain 756) did not cause any significant symptoms on the hosts. The mutant 756 induced visible symptoms on the cultivars Cherokee and Inka. On Cherokee there were small watersoaked lesions, and HR (hypersensitivity reaction) was detected on Inka, but this was restricted to some cells only (mikro HR). The rate of multiplication of the wild type strain was much higher than the multiplication of the mutants. Bacteria were detected in the cotyledons and primordial leaf, but there is not any substantial number of bacteria in leaves, except for strains 757, 1212 and 1213. The rate of multiplication of strain 756 was intermediate. These, and other experiments can help to understand the genetic background of resistance and the host-pathogen relationship in the Pseudomonas-bean pathosystem.
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Optimization of fertilizer use efficiency, soil quality and oil palm (Elaeis guineensis Jacq.) growth with biochar under drip irrigation conditions irrigation conditions
31-36.Views:86Biochar (BC) is an eco-friendly product characterized with high content of carbon and usually obtained by heating biomass without oxygen. Utilizing BC as organic material to amend the problematic soils and improve plant growth and yield has been proved previously. This study investigated the effect of vetiver grass biochar (VGB) on fertilizer use efficiency, soil quality, and oil palm growth performance. A net house experiment was conducted at the Farm Unit, UiTM Sarawak Branch, between August 2022 and March 2023. A factorial randomized complete block design (RCBD) with five treatments and four replications was devised. Treatments applied were: T0) absolute control; T1) 100% NPK fertilizer; T2) 100% vetiver grass biochar; T3) 50% vetiver grass biochar + 50% NPK fertilizer; and T4) 25% vetiver grass biochar + 75% NPK fertilizer. The BC application significantly improved the fertilizer use efficiency through reducing the rate of fertilizer applied. It also significantly enhanced most soil-measured chemical properties and soil nutrients. The growth performance of oil palm plant was significantly enhanced by BC application in terms of plant height, hump diameter, leaf number, chlorophyll content, and plant biomass. The BC application demonstrated its usefulness in managing soil and cultivating oil palm plant sustainably by reducing the rate of fertilizer applied and improve the fertilizer use efficiency. Based on the output, we suggest that treatment T3 (50% vetiver grass biochar + 50% NPK) can be used to improve the growth performance of oil palm.
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Comparative investigation on Hypericum perforatum L. populations of different origin
56-60.Views:223Widespread application for herbal medicines based on Hyperici herba has been experienced in the last few years, especially in the treatment of depression syndrome. As the wild origins could not satisfy the market demands neither in quantitative nor in qualitative respect, the necessity of the development of intensive growing methods has been raised. In the course of our investigations we intended to clear up the morphological and chemical variability among and within populations of different origin in order to start a new breeding program. According to our data, growth dynamics of populations could be characterised by a logistic curve. According to the time of flowering the populations formed early, middle and late groups. Morphological diversity among populations was measurable in differences of flower length, plant height, different leaf types and plant habit, according to which characteristics groups were distinguished. Generally, the accessions were the most homogenous in plant height (CV: 7-15%), followed by inflorescence-lengths (CV: 11-36%) and the least uniform characteristics proved to be the number of flowering shoots (CV: 14-59%). The greatest morphological heterogeneity was experienced in the accessions of wild origin as it has been expected. In the second vegetation period generally a much better homogeneity was obtained, than in the first year. The most outstanding accessions produced 1.2 t of dry flowers and 4 t of dry flowering shoots, calculating to one hectare area. The individual yields varied on a large scale in each population (CV: 18-70%).
The content of hypericin varied between 1,85 and 9,9 mg/g in 1996, and between 0,18 and 2,70 mg/g in 1997, showing high individual divergences. Flavonoid values -including first of all hyperosid, rutin and quercitrin - reached 17-39 mg/g in the first and 15-20 mg/g in the second year, respectively. Individual and seasonal variation was less than in hypericin. A joint high level of these two compound groups seems to have low frequencies.
The superior population were selected for further breeding.
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Nutritional aspects of producing fruits organically
69-74.Views:373Recent interest to avoid use of agrochemicals in fruit growing to safeguard environmental and human health has stimulated interest in organic fruit production (OFP) all over the world. Organic production requires a holistic approach to agricultural ecosystem management. Because of the perennial nature of orchards, this is not a great departure from conventional orchard management, except that corrective techniques are limited primarily to naturally derived materials. Organic mineral nutrition management hinges on two principles: 1) practices that lead to the build up and maintenance of soil that is biologically active and high in organic matter; 2) supplementing the mineral nutrients provided by the soil with fertilizers from approved sources. Organic orchards should be sited on land with superior soils and preplant soil preparation to increase organic matter and correct any sub-optimal soil characteristics. For successful organic fruit growing the following statement should be considered:Weed management is critical to reduce competition for nutrients and water. Soil and leaf analysis provide the basis for correcting mineral nutrient deficiencies or imbalances in organic production. It may be necessary to use a number of strategies to supply mineral nutrients over the life of the orchard. The slower, natural methods applied require a management approach that is simultaneously patient and dynamic.