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Determination of the validation parameters of inductively coupled plasma mass spectrometer (iCP-mS): response curve linearity in the case of arsenic and selenium
67-71Views:131In the field of elemental analysis inductively coupled plasma mass spectrometers (ICP-MS) have the best sensitivity that means the lowest limit of detection, subsequently their applicability for the detection of essential and toxic elements in foods and foodstuffs is prominent. For the most elements could be measured the detection limit is between μg kg-1 (ppb) and ng kg-1 (ppt) e.g. for arsenic and selenium.
Considering an analytical task (sample type, analytes and their concentration, pretreatment procedure etc.) the applicability of an analytical method is determined by its performance characteristics. The purpose of validation is to ensure that the method would be used fulfills the requirements of the given task. In this article we describes one of the performance characteristics, the linearity, and the whole validation procedure aims measurement of arsenic and selenium in foodstuffs by inductively coupled plasma mass spectrometer (Thermo XSeries I.); but because of the limited number of pages the results are demonstrated only for arsenic.
The linearity of calibration was evaluated in three concentration ranges (0.1–1 μg l-1; 1–10 μg l-1; 10–50 μg l-1), with nine line-fit possibilities (without weighting, weighting with absolute or relative deviation; with or without forcing the curve through blank or origin) and different methods (graphical examination, correlation coefficient, analysis of variance).
The best method to ensure the linearity of correspondence between signal and concentration was the ANOVA test. In view of calibrations it was found that the range of 10–50 μg l-1 could be regarded as linear with four line-fit possibilities, and was non-linear between 0.1–1 μg l-1 and 1–10 μg l-1.
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Interaction of yield stability and year in major agricultural crops
41-46Views:85The effect of hydro-meteorological extremities on plant cultivation is the result of the correlation of many factors. These may increase or decrease the effects of hydro-meteorological extremes. The degree of this variance depends on the professionality of treatments, on the quality of the applied technique and technology and also on the soil’s water management characteristics.
The water management characteristics of Hungary’s arable land are mainly unfavorable or medium. In the past two decades the conditions of originally good soils, from a water management aspect, have significantly deteriorated in the critical 0-60 cm soil layer. This is mainly due to unprofessional land use, a lack of deepening cultivation and neglected organic cultivation. At the same time, hydro-meteorological extremities occur more frequently and the sensibility of plant cultivation has increased.
The sensibility of plant cultivation is type and location specific, yet, it also effects both the quality and quantity of the result.
The stability analysis, which covered the period of four decades and incorporated 6-7 agro-ecological areas proves and highlights the following:
• Winter wheat only reacted to extensive cultivation and unfavorable environmental conditions to a small degree. On the other hand, the effect of hydro-meteorological extremities increases.
• The stability analysis of maize, which is sensitive to cultivation technology and the location of cultivation, proved just the opposite. Good soil and adequate technology significantly reduces the effect of any particular year.
• From the years examined, the most favorable proved to be the one with average precipitation. Maize reacted to both extremities in a similar way. Winter wheat reacted to more precipitation with less yield.
• The yield quality of winter wheat was negatively effected by drought. The negative effect of precipitation is limited to the period of ripening and harvest, so the likelihood of such an effect is not significant.
• The yield of sunflower – due to pests – significantly reduces in years with high precipitation, while a difference between dry and average years cannot be pinpointed out. The oil content in both dry years and in years with high precipitation is evident, compared to years with average precipitation.
• The root yield of sugar beet is reduced by drought while the sugar content depends on soil characteristics and climatic extremities. A difference could also be noted by location, whether in Western Hungary and on the Great Plain. Great sugar content can be achieved in years with high precipitation in Western Hungary, while the same result occurred with average precipitation on the Great Plain. Drought did not have a positive effect on sugar content in either location. -
The analysis of the milk production and the body condition on two Saanen goat-farms
53-59Views:79The authors carried out an examination on two Saanen goat farms during four months from May to August 2008. The body condition score and milk production of the goats were measured. In data processing analysis of variance was used to compare the data of the studied farms. They tried to find the answer for the question how the body condition scores and milk production change in each month. The changes observed in each farm were examined separately then they compared the values of the two farms.. On one of the farms the nutrition was proper therefore with an ideal, intermediate body condition (average 2.73) the goats produced a good amount of milk (2.5 milk-kg/mother/day). On the other farm the nutrition was not proper, therefore the body condition of the goats was weak (average 1.88). Their milk production decreased significantly from month to month (average 2.35 milk-kg/mother/day). The authors could conclude considering both farms that the more milk the goats produced, the lower their body condition score became. The two parameters showed negative correlation.
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Effects of production factors on the yield and yield component of winter wheat
26-31Views:77The effect of major production factors (forecrop, fertilisation, irrigation, soil cultivation and soil preparation) on the yield components and yield of winter wheat were studied in a long-term experiment set up at the Látókép Experimental Nursery of the Agricultural Sciences Centre of the University of Debrecen. The results of regression analysis led to the following conclusions:
• In our experiments in 2000, after using maize as a forecrop –based on the results of analysis of regression – fertilisation determined the yield.
• After using pea as a forecrop, a N50 P35 K40 kg/ha fertiliser rate led to an economical increase in the yield of winter wheat.
• None of the determinative yield components varied significantly for winter wheat produced after using pea as a forecrop.
• There is a closed, significant correlation between plant height, spike length, plant and spike mass, the number of spikelets and grains per spike after using maize as forecrop. The thousand grain mass is different from the other yield components, because it is not part of the relation system of
those yield components.
• The increased yield of winter wheat after maize has been used as a forecrop is due to the positive change in grain number per spike yield component. -
The effect of the plant density for the yield of the maize hybrids
50-61Views:87In order to enchance the yield stability of maize, the effect of plant density on yields was studied on a typical meadow soil in Hajdúböszörmény between 2002-2004. In the plant density experiment, we used the method of Béla Győrffy. The plant densities applied therefore 20 to 100 thousand plants/ha by ten thousand scale. The application of fertilizer rates for the maize hibrids in every year were N: 110 P: 90 K: 120 kg/ha. We used a manual soiling-gun in the experiment. In every year we used plant protection techniques against monocotyledonous and dicotyledonous weeds. The harvest was done by hand. The facts were read by variancie analysis and linear regression analysis. The moisture and the temperatures were extreme in 2002, 2003, 2004. We have to mention defficiery of moisture in 2003 which is shown that the hot days number increased. After evaluating our findings we can conclude that most hybrids showed a significant correlation between increased plant density and the volume of yields. On the basis of the experiments we divided the hybrids into four groups: the first group included the hybrids suitable for increased plant density with a wide range of optimal density values; the second group included hybrids, which did not require high plant density, were capable of good individual performance and tended to grow several ears; the third group included flexible corn types, which grew longer ears in favourable years, thus yielded more; and the fourth group included the hybrids, which were sensitive to increased plant density and which showed a narrow range of optimal density values. Finally, plant density determines the yield; we have to consider optimal plant density intervals as well as optimal plant density, and we also have to place a high emphasis on the use of hybrid-specific technologies.
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Correlation analysis of relative chlorophyll content and yield of maize hybrids of different genotypes
211-214Views:103In 2021, correlation between relative chlorophyll content and yield in three maize hybrids of different genotypes was examined. The data were collected at the Látókép Experimental Station of the University of Debrecen located on the Hajdúság loess ridge in Hungary. The soil of the small plot field strip plot trial, which was set up in 2011, was calcareous chernozem. Apart from the control treatment (without fertilisation), N fertiliser is applied in the form of base and top dressing. The base fertiliser containing 60 and 120 kg ha-1 N of nutrient applied in spring was followed by top dressing containing +30–30 kg ha-1 N in V6 and V12 phenophases. SPAD values measured at different phenological stages of the growing season increased by an average of about 28% up to 10 leaf stage for all three hybrids. In the pre-silking period (Vn), the relative chlorophyll content decreased by 8% on average. After an average increase of 14% in the tasselling and silking period, SPAD decreased by an average of about 29% at full maturity (R6).
For the different fertiliser treatments, higher N doses resulted in higher yields. In the basal fertiliser treatment, the A 60 N dose resulted in an average 34% increase in yield, and the A 120 N dose resulted in an average 94% increase in yield compared to the control. The 60 kg ha-1 N basal fertiliser (A60) increased in the V6 phenophase with an additional 30 kg ha-1 N resulted in an average yield increase of 26%. When 120 kg ha-1 N of basal fertiliser (A120) was increased by an additional 30 kg ha-1 N in the V6 phenophase, only the Merida hybrid showed a significant yield increase (7%). No further yield increase was observed when V690 and V6150 treatments were increased by an additional 30 kg ha-1 N in the V12 phenophase. The yield of the Armagnac hybrid decreased by almost 20%, the yield of Fornad by 3% and the yield of Merida by 1%.
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Effects of long-term K fertilization and liming on the extractable and exchangeable K contents of a Haplic Phaeosem soil
141-145Views:30Effects of regular K fertilization and liming on the easily extractable K content of a Haplic phaeosem soil determined in 0.01 M CaCl2
and AL (traditional method in Hungary) were examined in the B1740 type of the National Uniformed Long-Term Fertilization Experiments
at Karcag.
Close correlation (r=0.95) was found between the 0.01 M CaCl2 and ammonium lactate - acetic acid (AL) extractable K contents of
soils.
K fertilization increased the amount of 0.01 M CaCl2 and AL extractable K significantly. Liming had different effects on the amounts of
K extracted by these two methods. Liming increased the amount of AL-K and decreased the amount of CaCl2-K. CaCl2 extractable K was in
close correlation with the relative amount of exchangeable K content of the soil (K%) and the agronomic K balance. The results of regression
analysis confirmed that the CaCl2-K characterized K% and the AL-K related to the absolute amount of exchangeable K.
On the basis of the presented results it can be stated that the 0.01 M CaCl2 is able to detect not just the increase of easily extractable K
caused by fertilization and liming but the changing of the rate of the relative amount of exchangeable K. -
Effects of agrotechnical factors on the quality and quantity of yield in winter wheat production
69-75Views:138The present study was conducted to determine the effect of basic agrotechnical factors on the yield and quality of winter wheat. Two experiments were set in 2017/2018 growing season, where we studied the influence of different forecrops, fertilizing treatments and cultivars. 204 samples were measured with Single Kernel Characterization System and NIR grain analyser to determine protein (NIR-P), wet gluten (NIR-WG), Hardness Index (HI), kernel weight (KW) and kernel diameter (KD). Fertilizing had a significant effect on yield, KW, HI, NIR-P and NIR-WG, except KD. N90PK dosage was enough to realize yield potential for 6 out of 9 cultivars, but considering protein content N150PK dosage was needed. The forecrop had no significant influence on yield, KW, KD or HI, however sweet corn as previous crop had significant improving effect on NIR-P and NIR-WG compared to sunflower as forecrop. According to our data of correlation analysis, no negative relationship was found between yield and NIR-P, however HI was in medium positive correlation with NIR-P. The variety Vyckor had the highest yield, but in quality aspect, the highest NIR-P and NIR-WG values belonged to KG Kunhalom variety.
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Evaluation of Soil Degradation Based on High Resolution Remote Sensing Data
145-148Views:77Soil salinity is the main problem of soil degradation in the Grate Plain with cultivated area of 20% affected. Its influence is accelerated on the water managed and irrigated lands. Remote sensing can significantly contribute to detecting temporal changes of salt-related surface features. We have chosen a farm where intensive crop cultivation takes place as a test site as soil degradation can be intensive as a result of land use and irrigation. In order to evaluate soil salt content and biomass analysis, we gathered detailed data from an 100x250 m area. We analyzed the salinity property of the samples. In our research we used a TETRACAM ADC multispectral camera to take high resolution images (0,2-0,5 m) of low altitude (300-500 m). A Normalized Vegetation Index was computed from near infrared (750-950 nm) and red (620-750 nm) bands. This data was compared with the samples of investigated area. Analyzing the images, we evaluated image reliability, and the connection between the bands and the soil properties (pH, salt content). A strong correlation observed between NDVI and soil salinity (EC) makes the multispectral images suitable for construction of salinity map. A further strong correlation was determined between NDVI and yield.
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Nutritive value of Timothy (Phleum pratense L.) during primary growth in 2005
61-67Views:94In this paper we analysed the change of the chemical composition and nutritive value of Timothy observed during the spring of 2005. The nutritive value of Timothy was observed between the end of April and the beginning of June relating to the following parameters: crude protein, crude fibre, crude fat, ash, N-free extract, net-energy growth, net-energy lactation, net-energy maintenance, Metabolizable Protein Energy dependent, Metabolizable Protein N-dependent. We also analysed whether a relationship between the environmental factors that affect the
growing period of grasses and the chemical composition can be detected or not. While testing for correlation, the number of days from 1st January, the amount of heat accumulation, solar radiation and rainfall were considered as independent coefficients.
For the estimation of weather conditions we calculated the climate index. The observed year can be described as a year with a rainfall above the average and abundant solar radiation. A correlation can be detected between the change of parameters of nutritive value and the quality of the current year. In 2005 the result of the analysis of nutritive value was showed a significance difference with respect to each chemical composition at the rate of P<0.001 depending on the time when the samples were taken.
According to the outlined data it can be stated that the change of the value of crude protein and ash show decreasing tendency agreeing the research literature. In parallel with the change of nutritive value, the amount of crude fibre and N-free extract increased. However, the value of Net-Energy maintenance showed an alteration only in the first half of the observed period. The same alteration tendency can be detected in the Net-Energy growth and the Net-Energy lactation. -
Yield and crown structure characteristics in a red oak (Quercus rubra L.) stand: Case study
49-53Views:109The paper provides the results of a detailed analysis of timber volume and several important crown variables of red oak (Quercus rubra L.) based on an experimental plot in eastern Hungary. At the age 32 years the crop trees belonged to different height classes. If the volume of the mean tree from height class I was considered as 100%, the volume of the mean tree of class II was 59%, and the mean tree of class III was only 36%. It appeared that there was a significant correlation between crown indices and yield. For this reason, diameter at breast height showed a positive linear correlation with crown diameter (R2= 0.6211). Additionally, there was also positive linear relationship between crown diameter and volume (R2= 0.6908). The variation of crown indices is height even within the same stand and indicates the importance of following a selective thinning operation method.
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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
112-118Views:101The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method with a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction. -
Evaluation of KCl-EDTA-, CaCl2- DTPA-, CaCl2-DTPA-TEA extractants for estimating available Cu in soils
5-9Views:104KCl-EDTA-, CaCl2-DTPA-, CaCl2-DTPA-TEA extraction
methods were compared to determine the plant available Cu
amounts in the soil samples. In the soil extracts, the amounts of
copper were measurable. In the buffered CaCl2-DTPA-TEA
extracts, data showed the lowest values.
On the applied soils, a correlation was observed between
plant removal and soil Cu using extractants mentioned above. The
closest correlation appeared in the case of sandy soils.
From the results of statistical analysis, it appears that soil
properties may play role in the efficiency of the extraction using
either CaCl2-DTPA or KCl-EDTA solutions.
To establish more reliable relations, further studies with
different types of soils are needed. -
Using phenotypic correlations in the breeding evaluation of Hungarian Sport Horse mares
38-43Views:103The aim of this paper is to analyse phenotypic relationships between self performance test (SPT) parameters of Hungarian Sport Horse mares. Establishing these relationships may contribute to develop a breeding value estimation model. Data were given by the Association of Hungarian Sport Horse Breeders.
The analysis is based on the SPT results of 3 and 4 year old mares from 1993 to 2004. Our aim is to determine practically explainable correlations among conformational traits (type, head, neck, saddle region, frame, forelegs, hind legs, regularity of movement, impulsion and elasticity of movement, overall impression); free jumping performance (jumping style, jumping ability, observation during training) and movement analysis traits (walk, trot, canter, overall impression, test rider’s score). A further aim is to determine correlations between free jumping performance and movement analysis traits. -
Utility of Winter Wheat According to Visual and Microbiological Fusarium Infection, as Well as Toxin Examination
26-34Views:90In 1998 the Fusarium infection was studied visually and microbiologically and also F-2, T-2, HT-2, DAS and DON contamination were measured using 22 winter wheat samples. The correlation between the different parameters of 22 wheat samples were determined by regression analysis. According to our results we can state the following.
There is no significant connection between the results of visual, microbiological and toxicological examinations. This means that no certain conclusion can be drawn about the toxin contamination of samples – which is a determining factor of its utility – based on the visual symptoms and the plate dilution method.
Our results indicate however – though it is not proven statistically – that those samples in which the Fusarium infection did not exceed the limit of the standard, also had low toxin contamination, therefore they can be used as components of forage.
It is a considerable problem, however, that according to the visual qualification, such samples are excluded from the later utilisation, wherein the toxin contamination does not justify such action. Therefore, it is necessary to examine the toxin content of those samples which show high infection by visual symptoms. To reducing the number of expensive toxin examinations it would be advisable to change the currently used 0,5% limit which is indicated in the standard for a higher value of infection, for example to 2%, as recommended by Mesterházy (1998). -
The possibility of use of the 0,01 M CaCl2 and Baker- Amacher extractants for the determination of plantavailable potassium
7-15Views:72The Hungarian fertilizing recommendation systems use AL soil test for the evaluation of potassium supply. The 0.01 M CaCl2 is a definitely milder extractant, it extracts the easily soluble and exchangeable potassium amount. Its European introduction was already taken into consideration in 1994. The research project on this topic is started in several european countries, also in Hungary at the Department of Agricultural Chemisty of Agricultural University of Debrecen. Another advantage this multielement method is that the different element-ratios can also be calculated.
The Baker-Amacher extractant’s principle is that it contains a known amount of K, P, Mg in the CaCl2 solution. During the soil extraction adsorption and desorption process take place, so the adsorption or desorption can be calculated from the original and the final concentrations.
In this paper we introduce the results of comparing analysis of the samples (n=630) from Soil Information and Monitoring System. Our aim was to measure the use of new extractants beside conventional extractant (AL) for the evaluation of K-supply would be reasonable.
It can be stated that there is a medium close relationship (r=0.75) between AL-K and 0.01 M CaCl2-K. My calculations confirmed the results of former examinations, and proved that the two extractants don’t extract and change the same rate of K-fractions. We found that regression between 0.01 M CaCl2 and AL depend on texture classes, pH classes, amount of lime, and organic matter content of soils.
Comparing the relations between AL and Baker-Amacher we find relatively loose correlation (r=0.45). We stated that there are K-fixing soils among soils considered to be well supplied with potassium by AL. This might be caused by the high amount of mineral clay and the quality of mineral clay. We stated that the dK averages show that the Hungarian nutrient-supply categories characterize generally well K-supplement of soil.
It can be stated that it would be necessary to use new extractants to specify evaluation of plant available K. We found that the 0.01 M CaCl2 and Baker-Amacher extractants could complete usefully the AL procedure and could help effective potassium fertilization. -
Examination of compost maturity using reflectance
29-34Views:122Composting is one of the most popular recycling processes for organic waste. Composting plays an important role in waste and by-product management and is becoming increasingly important in both sustainable energy management and circular economy. Composting transforms organic matter to produce a safe and stable by-product (compost) that can be applied to arable land in a similar way to fertilizer. Physical, chemical and biological methods can be used to monitor the process and to determine the maturity of the compost, as spectrometric/spectroscopic methods play an important role in the analysis of different environmental samples.
Our aim was to (1) non-destructively detect the effects of different additive ratios on the spectral properties of the composting process and the spectral data of different compost mixtures, (2) to find the wavelength ranges of the reflectance curve (inflection points) sensitive to compost maturity, (3) to determine the correlation between the inflection points and the chemical and physical parameters measured in compost by conventional methods.
The mixture of broiler and hen manure and zeolite was composted 62 days in windrow composting. In the composting experiment, the moisture content and temperature (°C) were measured every three days and compost samples were taken and in 10% destillated aquaeous suspension were measured the pH and electrical conductivity (mS cm-1). Compost samples dried to mass stability were spectrally analyzed in the wavelength range 400–1000 nm with AvaSpec 2048 spectrometer.
Based on the results, the reflectance of mature compost were smaller in the last days of composting than the reflectance values of day 0 samples, thus compost maturity can be detected spectral in the VIS-NIR wavelength range. For the tested compost prisms, the reflectance of each sampling day shows a constant slope, with a significant overlap of the reflectance curves up to 400–700 nm wavelength range, and there was a breakpoint in the 700–750 nm wavelength range which was proved by binary encoding.
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Effect of Soil Covering on the Soil Enzyme Activity of Integrated Orchard
20-29Views:82The purpose of our experiments is to discover the effect of different soil cover matters (agrofoil and black polyethylene) on the activity of some enzymes (phosphatase, saccharase, urease, catalase, dehydrogenase) occuring in soil. Soil samples were taken from a cider apple plantation of the Fruit Producing Research and Advisory Kht Újfehértó. The enzyme activity was measured according to Krámer and Erdei (1959a), Kuprevič and Tsherbakova (1956), Kuprevič et al. (1966), Frankenberger and Johanson (1983), Mersi and Schinner (1991). Soil moisture content was by conventional (drying chamber) method measured during every sampling and enzyme activity was transpolated to absolute dry soil. Results were estimated by mathematical methods (variation analysis, correlation counting). Soil samples were taken by trials 5 times (in every two months) a year in the vegetation period from March to November.
By recording the monthly changes of the enzyme activity we have observed the following. The activity of the phosphatase was generally the highest in May and the lowest in November. Depending on the trials, high values were also measured in March and September. The activity of the saccharase was generally the highest in November and the lowest in June, but at the same time peaks even occured in May and September. The highest urease avtivity was measured in September and November, and the lowest activity in May and July, also depending on the trials. In the year 2000, after a deep point in March, the activity of the catalase was the highest in November or by certain trials in September. In 2001, the lowest activity was also measured in March, but the highest activity appeared in November in case of one-minute trial, and in May in consequence of two-minute trial. Finally the activity of dehydrogenase was the highest in November and the lowest in July apart from the model years.
There were essential differences in rainfall of the two experimental years which was reflected in the enzyme activities. There was a poor positive significant relationship between soil moisture content and enzyme activity values in case of phosphatase, saccharase, urease (r=0,426; 0,480; 0,396) respectively. In case of catalase1 (r=0,518), catalase (r=0,556), dehydrogenase (r=0,559) we obtained a medium strong positive relationship between soil moisture content and enzyme activity values. By evaluating the effect of different trials in case of every examined enzyme significantly higher values were detected in soils covered by agrofoil (a porous black polyethylene) than in soils covered by black polyethylene or in uncovered soils. Moreover, the soil covered by black polyethylene showed significantly higher enzyme activities (besides phosphatase) than the control soil. Thus soil-covering meant statistically significant advantages in enzyme activity as opposed to uncovered soil proved. -
The correlation of foreign language proficiency, employment and regional competitiveness in the North Great Plain Region of Hungary
74-82Views:59Employment is one of the basic factors when evaluating a region’s competitiveness. Boosting employment figures will also increase individual income. However, the creation of jobs alone will not solve the problems of unemployment, as labour force with skills matched to labour market demands should also be available. Continuous development of the labour force, adjusted to recent needs in the labour market, can contribute to a region’s competitiveness. Mismatches between education and labour market requirements would be too expensive, especially for backward regions like the North Great Plain Region of Hungary. Foreign language education should also be adapted to the changing economy when selecting target languages and language skills. A workforce equipped with contemporary and needed second language skills can be an element in making a region attractive for investors. Although current trends show signs of improvement, Hungary and especially the North Great Plain Region still does not have much to offer in this respect. The paper examines the reasons for the low level of second language acquisition among Hungarians and the population of the North Great Plain Region and explores the ways foreign language knowledge could promote economic development.
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Methodology adaptation and development to assess salt content dynamics and salt balance of soils under secondary salinization
199-206Views:142The effect of irrigation with saline water (above 500 mg L-1) is considered a problem of small-scale farmers growing vegetable crops with high water demand in the hobby gardens characteristic of the Hungarian Great Plain. In order to simulate the circumstances of such hobby garden, we set up an experiment including five simple drainage lysimeters irrigated with saline water in the Research Institute of Karcag IAREF UD in 2019. We regularly measured the electric conductivity (EC) of the soil referring to its salt content and the soil moisture content with mobile sensors. Before and after the irrigation season, soil samples from the upper soil layer (0-0.6 m) were taken for laboratory analysis and the soil salt balance (SB) was calculated. The actual salt balance (SBact) was calculated of the upper soil layer (0-0.6 m) based on the salt content of the obtained soil samples. The theoretical salt balance (SBth) was calculated by the total soluble salt content of the irrigation water and leachates. During the irrigation season, we experienced fluctuating EC in the topsoil in close correlation with the soil moisture content. Based on the performed in-situ EC measurements, salts were leached from the upper soil layer resulting in a negative SB. Combining SBact and SBth of the soil columns of the lysimeters, we estimated the SB of the deeper (0.6-1.0 m) soil layer. We quantified 12% increase of the initial salt mass due to accumulation. We consider this methodology to be suitable for deeper understanding secondary salinization, which can contribute to mitigating its harmful effect. By repeating our measurements, we expect similar results proving that saline irrigation waters gained from the aquifers through drilled wells in Karcag are potentially suitable for irrigation if proper irrigation and soil management are applied.
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The role of leaf area and photosynthesis in optimising the sowing date of maize
5-10Views:120Our sowing date experiment took place in the Demonstration Garden of Institution of Plant Sciences, Agricultural Center of University of Debrecen, in 2012–2014. The thesis contains data of test year 2014. Our purpose, besides several other examinations, was to observe how sowing date influences leaf area index and activity of photosynthesis of maize hybrids, and how those factors affect fruiting. In the experiment we monitored the change of the leaf area index and the photosynthesis of hybrids with four different growing seasons.
Based on the results, it can be concluded that most of the examined hybrids reached their smallest leaf area with the third sowing date and with the highest yield results. Hybrid Da Sonka had the largest leaf area (4.10 m2 m-2), and hybrid DKC 4590 produced the highest yield (13.16 t ha-1)
with the third sowing date.
During testing the photosynthetic capacity, the extremely high performance of the youngest plants with the third sowing date is outstanding, which can be explained by the different ripening periods. Examination of the correlation between the photosynthetic capacity and the yield, by linear regression analysis, also proves that photosynthesis has a determinative role in fruiting.
The results obtained confirm that not only the environmental and agricultural factors in the growing season have effect on the yield, but also other factors like the leaf area index and the photosynthesis are determinative parameters, and all those factors together, modifying effects of each other, develop average yields.
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Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)
49-56Views:246In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to improve immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.
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Examination of Reproductive Performance of Roe Deer (Capreolus capreolus) in Hungary
33-38Views:93The objective of the research partly is to compare the reproduction performance of the populations living in different regions with regard to some special characteristics (age, condition).
When estimating the age through tooth wear and cementum-layer-counting there was a difference of 0.87 years in favour of the first one (r=0,840; p<0,001). I found cementum layers at 42% of the does in the study after examining the MI teeth.
There was lose connection between the weight (eviscerated, with head and legs) and the KFI (r=0,296; p<0,01), and for further analysis, I used only the KFI as the index for condition.
The regional average KFI varied from 0.24-0.37 in fawns, 0.82-1.73 in does, with individual extremes of 0-4.05. Within the examined regions the highest index belonged to the prime-aged does, while the 1-year-olds had a lower rate, and it was the lowest in the does older than 8 years.
The rate of fertility was between 83,3(ns)-100% as we can see from the presence of the CL. All the examined does were fertile, except in one region, while among the female fawns in two regions I only found three with active ovaries. The average number of CL was 1.5-2.13, and this varied by regions; all in all it was the highest in the 2-7-year-old group (1.96) and in the ones over 8 years (2.00!), while it was lower in the does younger than 1 year (1.90). The high fertility of the does over 8 years is remarkable.
I could examine the number of embryos in two regions during the post-implantation period, and beside 100% fertility I found significant differences among the does, which can be associated with the condition. The ratio of CL carriers and the pregnant does was 100% and 73% in the two regions, the average number of CL were 1.92 and 1.72, while the average embryo number were 1.83 and 1.36 per doe. The difference between the CL and the embryo numbers on the two regions were 5% and 21%. The difference (prenatal loss) is in connection with the age (age class) of the doe. It is possible, however, that in some cases oestrus was not followed by gestation. But in roe deer, owing to the commonly known lack of luteolysis-mechanism (Flint et al., 1994), the regression of the CL of the does that did not get pregnant takes place in December and January, so the CL found in January cannot prove a previous pregnancy, which might have been followed by an abortion.
Although it has to be proven, it seems that the number of the CL (potential progeny) can be associated with the age (r=0,418; p<0,01) and the weight (r=0,312; p<0,01) of the doe, while the embryo number (realised progeny) is influenced by the age of the doe and probably by external factors.
It is essential to continue and extend the research to increase the reliability of the results and their correlation. -
Energy crops on less favoured (alkaline) soil
115-118Views:99The reduction in fossil energy and row material sources induces growing demand for renewable resources. The growing demand for herbal raw materials has land use impacts as well. One way to reduce the conflict between the food and energy crops can be the utilization of less favored areas by growing energy crops. Among the potentially available areas for this purpose the salt affected soils (SAS) occupy a significant territories. SAS with structural B-horizon (meadow solonetz soils) represent the most wide spread group of SAS in Hungary. About half of these soils have been reclaimed and used as arable land and the remaining 50% are used as grassland. Sweet sorghum production for manufacturing of alcohol production was investigated in a long term amelioration and fertilization experiment on a salt affected soil (meadow solonetz). By means of regression analyzes the effect of sodium content of the soil and increasing mineral fertilizer doses were studied. According to the multiple regression analysis only the effect of nitrogen fertilizer was significant. On the solonetz type salt affected soil the effect of water soluble salt content of the soil was not significant, but there was a closer correlation between the ammonium-lactate sodium content and the yield of sweet sorghum. The maximum green mass was 45–50 t ha-1, in the case of low Na content and high level of nitrogen fertilization.
In order to quantify the potential yield of natural grass vegetation the relationship between the soil forming processes and the grass vegetation
was investigated. Beyond the different forms of Na-accumulation, the spatial pattern (mosaic-like characteristic) is also an inseparable feature of salt affected soils. The difference in the water regime, caused by the micro-relief is the main cause of variability. The run-on water keeps the deeper parts of the catena position wet longer. The wet situation causes more intensive leaching. In the low-laying parts of salt affected soils species preferring wet situations (mainly Alopecurus pratensis) are in majority. On the higher parts of the micro-relief species tolerating dry situations (mainly Festuca pseudovina) are dominant. The yearly grass production of low laying areas can be 4–7 t ha-1 but because of prolonged wet conditions the grass is not grazed and mowing can only be in old state. This old grass is not proper for feeding, but it may be suitable as energy plant. -
Genetic Diversity of Hair Colour in Horses
40-45Views:76The main aim of this study was to analyze sources of variability for coat colour expressed as a quantitative trait as well as to determine non genetic factors influencing horse colour variation and shades. Traditional Hungarian horse breeds such as Lipizzan (grey), Gidran (chestnut), Shagya Arabian and Arabian Pure Bred (grey) and Nonius (bay and black) horses were involved in the current study. We report results using direct measurements of horse coat colour using objective colorimetric dimensions and a spectrophotometer for determining hair melanin. Correlation analysis indicated that the single biological component that correlated best with the total melanin content of horse hair is the L* value (r=-0.858, p<0.0001). Subjective colour classes and coat colour variables L* and a* were obviously related, with R2 values of 0.88 and 0.46 respectively, with a lower R2 for b* of 0.22 (all p<0.001).