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Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia
74-83.Views:70Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.
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Variability and differences of growth vigour in the set of 36 genotypes of apricot (Prunus armeniaca L.).
30-34.Views:145Growth vigour of 36 apricot cultivars and new hybrids grafted on apricot seedling rootstock (Prunus armeniaca L.) was evaluated on the base of measurements of stem girth from the 411' to the 10th year after planting. There were differences in growth vigour of genotypes under study. In the evaluated set of genotypes the control cultivar 'Veecor may be classified as a genotype with below-average growth vigour. Only four genotypes (-Reale d'Imola-, Sanagian -Moldavskii krupnoplodnyl and 'LE-2385') were found with significantly higher growth vigour than that of control cultivar 'Veecot' in years of the end of experimental period. Two genotypes (Farmingdale', -LE-SE0-24') were found with significantly higher growth vigour only at the beginning of experimental period and one cultivar ('Vivagold-) with significantly lower growth vigour in the first four years. Genotypes with different growth vigour can be used in further breeding programmes and/or as components inhibiting or supporting the growth in indirect vegetative propagation. Within the whole experimental period, the rank of growth vigour of genotypes practically did not change. This was demonstrated by highly significant or significant coefficients of correlation existing between individual pairs of years (r=0.32+ to r=0.96++). As far as the time difference between years in individual pairs of years was higher, the correlation coefficients were lower. In individual years, variability of growth vigour was relatively low and ranged from 9.83 to 13.64%.
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Susceptibility of European pear cultivars to Venturia pirina Aderh. infection at a variety collection in Hungary
41-47.Views:293Field sensitivity of 271 European pear genotypes were evaluated in a large variety collection of pear in Hungary during a nine years survey. Substantial differences were detected in the rate of pear scab infection of leaves and fruits. Some 18 per cent of the inspected genotypes remained completely free of infection even in the years with weather favouring to the disease. On the other hand, 78 % of the inspected genotypes were infected in some or more extent. The rate of infection was generally higher on leaves than on fruits. 18.6 % of the genotypes inspected were tolerant to leaf infection and their majority (81.1 %) were tolerant to fruit infection. Some one fi fth of the genotypes (19.7 %) were highly susceptible to leaf infection but only minor rate of them (2.2 %) were highly susceptible to fruit infection. There were 44 genotypes that could be regarded as tolerant to pear scab leaf and fruit infection under fi eld conditions. Some of these genotypes may be suitable to commercial pear growing with highly reduced chemical control against pear but their yield capacity and fruit quality should be evaluated.
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Nutritional quality, fruit shape and relationships among exotic and local Capsicum pepper genotypes in Uganda
33-39.Views:356Twenty-one hot pepper genotypes comprising of local (15) and exotic (6) types (C. annuum, C. frutescens and C. chinense) were characterized for selected fruit traits after propagation in a glasshouse at the Makerere University Agricultural Research Institute Kabanyolo in Central Uganda using a completely randomized design with three replicates. Ripe fruits were harvested and analyzed; traits evaluated were all significantly different at P<0.05 with variations in quality attributes. The genotype OHA-B305-10 had the highest ascorbic acid content (128.86 mg/100 g) and is recommended for improvement of both local and exotic genotypes targeting the fresh market. Genotypes CAP0408-12 and UG2 WE0511-22, with highest total soluble solids (16.17 ºBrix) and dry matter content (28.59%), respectively should be used in improvements for industrial use or processing to products such as chilli powder or flakes. BRS-M205-04 with highest titratable acidity (1.04%) can be used in enhancing shelf life of genotypes with low titratable acids as well as for the fresh market. In spite of the intraspecific relationships among genotypes, significant differences were observed in their quantitative traits. These genotypes will, therefore, be useful in improving the quality of hot pepper fruit in Uganda.
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Inhibition and recovery of germination and growing ability of seedlings under and after osmotic stress induced by polyethylene glycol in 8 pea genotypes
53-59.Views:136Germination and early seedling growth of eight pea genotypes were examined under and soon after different (5%, 10%, 15%, and 20%) PEG treatments. Seeds were germinated on PEG solution for 3 or 6 days and then further germinated and cultured on filter paper moistened by water for 3 and 6 days. The length and fresh weight of shoots and roots of seedlings were measured and used for evaluation of genotypes. Roots were less inhibited by osmotic stress than shoots similarly to other plant species. The variability among the genotypes was the greatest in the case of shoot growth at 5% PEG treatment and in the case of root growth at 15% PEG treatment. Results suggest that growing responses of genotypes after cessation of stress are more suitable for the evaluation of their osmotic tolerance, than their responses expressed during in vitro stress conditions. Genotypes with relatively high or low osmotic stress tolerance, respectively, could be distinguished with 6 days after recovery from 3-day-long 15% PEG treatment concerning the rate of shoot weight to root weight.
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Effects of different pollination treatments in genotypes of Prunus salicina Lindl.
141-146.Views:128The low productivity in the Japanese plum (Prunus salicina Lindl) is related with self-incompatibility characteristics, so other species or varieties that act as pollinators need to be present to improve fruit production. The objective of this work was to study the efficiency of pollination in different genotypes of P. salicina using treatments of natural self-pollination, cross-pollination with P. armeniaca cv. Giada and open pollination. These treatments were evaluated through viability techniques and in vitro and in vivo germination of pollen grains; the growth of pollen tubes along the pistil was also observed. Genotypes used in this study showed differences for each one of the pollination treatments. Some genotypes showed signs of self-sterility and interincompatibility with P. armeniaca cv. Giada, while others showed partial self-fertility characteristics or pseudocompatibility. Moreover, some genotypes showed a higher affinity coefficient with cv. Giada and these will be indicating a possible intercompatibility. These studies will be an important contribution breeding and selection of intra and intercompatible genotypes to be used in commercial orchards.
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Reaction of different Capsicum genotypes to four viruses
61-64.Views:181The objective of this study was to examine the reaction of 44 Capsicum genotypes to common strain of Tobacco mosaic virus (TMV-C/U1), Obuda pepper virus (ObPV), NTN strain of Potato virus Y (PVYNTN) and legume strain of Cucumber mosaic virus (CMVU/246). Reaction (extreme resistance, hypersensitive reaction, latent susceptibility, susceptibility) of the tested Capsicum species/hybrids and breeding lines seemed to be greatly depending on hosts and viruses. Out of the breeding materials 4/99 F2 and IX-8 in to CMV-U/246, while 32.Bogyisz. type, VI-57 ii. 57/83 and V-12=19/98 to TMV-C/Ui showed extreme resistance. Two lines (V-25 F1=32/98 F1 and V-27 in F4=35/98 F4) showed hypersensitive reaction to ObPV. Latent susceptibility to PVYNTN was observed in case of all eleven tested Capsicum genotypes and in case of several lines to TMV-C/U1, ObPV and CMV-U/246. Other breeding materials proved susceptible to the mentioned viruses. Pepper genotypes showing extreme resistance and hypersensitivity could be used for resistance breeding to viruses.
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Evaluation of potential of fruit weight and fruit width and their interrelationships in the set of apricot genotypes
47-53.Views:136Within the period of 1994-1999, variability of fruit weight and width was evaluated in altogether 24 apricot genotypes. It is concluded that the genotype and climatic conditions of individual years are the factors causing variability in these two parameters of apricot fruits. Variability of fruit weight was significantly higher (approximately 3-times) than that of fruit width. When evaluated on the base of their width, 75.0% and 95.8% of apricots were classified into the groups of extra and first quality, respectively. As genotypes with the maximum fruit width (i. e. above 50 mm) the following cultivars were classified: 'Velkopavlovicka LE-6/2', 'NJA-1', 'M 45', 'M-25' and lednicka (M-90-A)'. The value of fruit width (in mm) corresponded with its weight (in g) only within the range of 40 — 45 mm. With the increasing and/or decreasing size of apricot fruit the changes in fruit weight were more pronounced than in those in fruit width. Within the set of genotypes under study, this relationship may be expressed by the equation y = 0.1234 — 7.6605 + 152.76; the corresponding values of correlation coefficient and coefficient of curve determination are r = 0.95-H- and R2 = 0.959.
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Susceptibility of European pear genotypes in a gene bank to pear psylla damage and possible exploitation of resistant varieties in organic farming
95-101.Views:270We evaluated 285 pear genotypes (commercial cultivars, ancient local varieties, unnamed local strains, seedlings, wild seedlings) in the largest gene bank of pear in Hungary from the point of view of psylla resistance to explore their possible exploitation in organic farming. We have found some 10 new resistant types (Bókoló körte, Bôtermô Kálmán, Füge alakú körte, Nagyasszony körte, Nyári Kálmán, Rozs nyári körte, Viki körte, Pb-242, Pb-299, 0-632) and 7 highly tolerant ones (Cure-6, Kései Kálmán, Kieffer, Kieffer Éd, Steiner, Téli Kálmán, II. B-3- 6/4, 96-16/5) (Table 1). These made up 3.5 + 2.8 per cent of the investigated genotypes, while 93.7 per cent of them were susceptible to pear psylla damage. Taking earlier and present results into account we can list more than 30 European pear cultivars being resistant or highly tolerant to pear psylla infestation and damage. In fact, the list of resistant and highly tolerant cultivars may serve as a basis selecting pear cultivars fitting to the specific requirements of the organic farming. By the end we can conclude that there is some real hope to exploit some resistant or highly tolerant ancient or local cultivars in organic farming but further investigations are needed to estimate their yield capacity and fruit quality.
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Rheological evaluation of industrial tomato in raw and processed condition
60-64.Views:233Tomato (Lycopersicon esculentum Mill.) is the second most important vegetable with one of the biggest producing areas worldwide, and an increasing tendency in Hungary as well. To fulfil the growing demands of the industry the producers must use the most suitable genotypes for the given conditions. In the experiment six genotypes (‘Heinz 1015 F1‘,‘Heinz 9478 F1‘,‘Kecskeméti 407‘,‘NUN254 F1‘,‘Prestomech F1‘ and ‘Rustico F1‘) were evaluated for different quality parameters: fruit shape index (length/diameter), firmness (g cm-2), force needed to tear the skin (kg-1) and consistency of tomato puree (g*sec). According to the results, the ‘Heinz 1015 F1‘ and ‘Heinz 9478 F1‘ were the most suitable genotypes for industrial processing in the mean of fruit quality. Strong positive correlation between consistency of juice and fruit shape index (r=0.891) and firmness of berry (r=0.882) was confirmed. The results have proved the necessity of these quality parameter measurements, which are important for the evaluation of raw material.
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Comparison of flower bud development in almond, apricot and peach genotypes
93-98.Views:242The phenological processes of flower bud development of stone fruits during dormancy are not thoroughly known. The yield of these species, especially of almond, apricot and peach is determined basically by dormancy of flower buds, the survival rate of buds during winter frosts and by their ability to develop normal floral organs in the next spring. After the initiation of floral primordia, flower bud development is taking place in continuous space until blooming, though at different speed characteristic to the species. To study flower bud development during dormancy we applied two alternative methods in different genotypes of almond, apricot and peach: (1) examination of pollen development (microsporogenesis), and (2) the measurement of pistil length. The samples were collected from the central part of Hungary during the dormancy period of 2004/2005. The three fruit species differed significantly in the speed of flower bud development, it was the quickest in almond, followed by apricot and peach. In addition to the species, there were significant differences in the process of microsporogenesis and pistil development between genotypes within species and also between the different types of shoots on which the buds were located. On short shoots buds developed at a higher speed, than on long shoots. Based on our observations, on the short shoots the period of endodormancy was shorter with 5-30 days, according to genotypes, compared to the long shoots. This difference, however, decreased to 2-3 days by the time of blooming.
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Evaluation of cauliflower genotypes by different growing method
11-14.Views:295Cauliflower is currently cultivated on 350-400 thousand hectares in the world and around 1500 ha in Hungary. Its cultivation requires considerable expertise as it is sensitive to environmental factors and adequate nutrient supply. The aim of our experiment was to compare three genotypes (Abeni, SV5777 AC and Telergy F1) by different growing method – under plastic tunnel and on open field. On open field the planting was on 19th of March, 2018, the row and plant distances were 45 and 35 cm. Under plastic tunnel the transplants were set out on the same day with the same genotypes as on open field, with 55x45 cm arrangement. The results have proved that cauliflower curds from plastic tunnel was two times bigger than on open field. The more protected environment had a positive effect not only on the mass of the curd, but also on the biomass production. Among the examined genotypes Abeni showed the highest yield (about 1 kg/plant) under plastic tunnel. The shape of the head is important for the market. According to our data, we measured the width and height of the curd. Open field conditions induced wider curd with longer stem and caused lower product quality and weaker correlation between the parameters than plastic tunnel.
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Fire blight (Erwinia amylovora) susceptibility of old Hungarian apple cultivars
35-38.Views:177The aim of the Hungarian apple breeding program started in the Department of Fruit Science was to find resistant apple cultivars against major diseases (scab, powdery mildew, fire blight). The outbreak of fire blight (Erwinia amylovora) in 1996 motivated us to search new resistant sources principally from old traditional apple cultivars. First of all, cultivars have been gathered since 1997 from Carpathia (Visk) and evaluated between 2001 and 2003. In this recent study evaluation of resistance of old Hungarian genotypes to fire blight collected from the English National Fruit Collection (Brogdale, Faversham) is presented.
13 old Hungarian apple cultivars in 2002, and 38 genotypes in 2005 have been evaluated. We used `Idared' and 'Jonathan M41' as susceptible controls and 'Liberty' and 'Remo' as resistant ones. Shoots of two-year-old potted plants were inoculated with a mixture of virulent E. amylovora isolates (Ea2, Ea60, Ea67) at a concentration of 5 x 108 cells/ml. Resistance of apple cultivars was evaluated weekly, four times after inoculation by disease severity of symptoms. Numbers of bacterial colonies in 1 cm length shoot were determined in the fourth week after infection.
8 cultivars in 2002 and 9 cultivars in 2005 displayed notable resistance to fire blight based on one-year data. Based on the coincident data of both years, out of the cultivars collected also from Carpathia ‘Pónyik', and `Sikulai' were found to be resistant and gene sources additional old Hungarian valuable apple genotypes could be selected: `Szabadkai szercsika' and `Tordai piros !davit'. The cultivar `Szemes alma' originated from Visk has proved to be recurrently resistant.
The number of bacterial cells in shoots of the investigated cultivars correlated with the severity of symptoms. With this method, which was introduced by us earlier, we can screen cultivars displaying weak visible symptoms, which cannot be proposed as a source of resistance because of their latent infection.
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Molecular identification of old Hungarian apple varieties
37-42.Views:273Altogether 40, mainly old Hungarian apple varieties were screened with six previously described microsatellite markers. A total of 71 polymorphic alleles were detected (average 11.8 alleles/locus) and the heterozygosity of markers averaged very high (0.8). The genetic variability among the genotypes proved to be so remarkable that as few as three markers from the applied six were enough to distinguish between the 40 varieties. This was also confirmed by the cumulative probability of obtaining identical allele patterns for two randomly chosen apple genotypes for all loci, which value was quite low: 2.53 x 10-5. The molecular identification of these genetically very different old apple genotypes could be very useful in future breeding programs.
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Agronomic and alimentary evaluation of elder (Sambucus nigra L.) genotypes selected from natural populations of Hungary
49-52.Views:170The black elder (Sambucus nigra L.) is a native plant in Hungary represented by extended and very variable populations. Cultivation of elderberry started during the late 90-ies because of the growing interests of the processing industry. High anthocyanin content, nutritive value of the berries and aromatic compounds of the flowers have been the esteemed constituents.
At the moment, there are about 3000 hectares elder plantations in Hungary, which is more than of any of the other European countries. Unfortunately, the cultures are planted to the Austrian selections of Haschberg, in spite of the fact that about 10-15 other selections of other foreign countries have been registered and planted elsewhere. In addition to that, the wild population of Hungary may offer a precious opportunity of further selection. Since 1970, Aladár Porpáczy at Fertőd and the Department of Fruit Growing of the former University of Horticulture in the 1980-ies started the selection in Hungary too.
The present study deals with 7 genotypes, their morphology of berries and inflorescences. Results are concentrated on four genotypes: Szcs1, Szcs-2, Szcs-3 and Szcs-5, which are all earlier ripening than the Haschberg varieties, and are superior in characters of fruit and inflorescence.
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Studies on the essential oil of different fennel (Foeniculum vulgare Mill.) populations during onthogeny
27-30.Views:228In the recent studies two Foeniculum vulgare Mill. populations (Hungarian and Korean) had been studied in open field trials in 1997-98 in Budapest for the identification of their essential oil characteristics. The essential oil accumulation level as well as the composition of the oil were studied during ontogenesis and at 6 phases of the umbel development from budding to overripening.
In the vegetative phases, the accumulation level of the essential oil was higher in the Hungarian genotype. In the leaf rosette stage, anethole is the main compound in both genotypes (40-96%), except the root of the Korean populations which contains 54% dillapiol. The accumulation level of anethole is slightly decreasing, while dillapiol is slightly increasing during the ontogeny. a- and [3-pinenes are characteristic compounds of the leaves, especially in the Korean genotype (10-11%). Before seed setting, fenchone was present in a considerable amount (7.5%) only in the umbels of the Hungarian genotype.
During the generative development, the maximum values of essential oil content are reached at the milky fruit stage (10,11%) in the Hungarian. and at the green fruit stage (7.1% ) in the Korean type, while the composition of the essential oil changed only slightly. The ratio of fenchone is increasing after flowering and being stable during ripening. Anethole varies to a smaller extent in the umbels, only.
We proved, that the dinamics of essential oil accumulation and the oil composition may vary according to genotype. Based on our results, the Korean population is characterized in the system of Bernáth et al. (2) as a high anethol-low methyl chavicol chemoform of the anethol chemovariety (fenchone<15%; anethole>68%; methyl chavicol< 3,2%).
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Characterization of quince (Cydonia oblonga Mill.) cultivars using SSR markers developed for apple
7-10.Views:386Quince (Cydonia oblongaMill.) is a minor fruit crop, which is primarily used for marmalade, jam and sauce.Very few quince cultivars are known all over the world and in many cases similar names are used for presumably different cultivars. The aim of the present study was to evaluate and characterize the genetic diversity of 36 quince cultivars and selections with SSR markers. Seven out of 8 SSR markers designed from apple sequences could successfully yield amplification also in quince cultivars. Number of alleles per locus ranged from 2 to 3 alleles. These allele numbers are quite low when compared to apple. It is supposed to be the consequence of a genetic bottleneck. In spite of the low allele number per locus, the 36 quince cultivars formed 30 different genotypes. The ratio of homozygosity was low, which might be coupled with the self-(in)compatibility phenotype of quinces. SSR markers proved unable to differentiate putatively closely related cultivars (e.g. ‘Bereczki’ and ‘Bereczki bôtermő’). In general, the level of polymorphism among the tested quince genotypes was much restricted due to the low allele number detected. However, it must be considered that the number of analysed SSR loci is not enough high to estimate the overall heterozygosity of the quince genome. Further experiments are needed and the SSR markers proved to be a reliable and useful tool for such analyses.
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Preliminary evaluation of selected Prunus spinosa and P. insititia genotypes for their nutraceutical properties
19-22.Views:280Fruits of nine Prunus spinosa and P. insititia selections were compared in their ferric reducing antioxidant power and total phenolic content. The antioxidant capacity and total phenolic content ranged between 6.36 and 29.26 mmol AA/L, and 5.04 and 29.71 mmol GA/L, respectively. These ranges cover an almost 5-times variation among the tested genotypes. The Pearson’s coefficient was very high (0.92) indicating a major contribution of polyphenolics to the antioxidant capacity of the tested Prunus fruits. Conserving resulted in an approximate 20 % loss of antioxidant power and slightly increased phenolic contents. Our results led us to the conclusion that fruit of Prunus spinosa and P. insititia might be considered as rich sources of antioxidants. In addition, procession with heat treatment caused only a slight decrease in the antioxidant capacity without loss in the total polyphenolic content.
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Agronomic evaluation of different lettuce (Lactuca sativa L.) varieties under unheated plastic tunnel
50-56.Views:295Among the leafy vegetables, lettuce (Lactuca sativa L.) is the most widely grown species in the world. Wide ranges of physical properties of the products are used to assess the degree of maturity. In this study, different lettuce varieties were grown under an unheated plastic tunnel in springtime. We evaluated the agronomic properties of different lettuce varieties, evaluated the condition of the plants, and determined the bioactive substances. Larger head weight can be achieved by the ‘King of May’ butterhead (259.31 g) and ‘Great Lakes 659’crisphead (220.40 g) genotypes. A very strong correlation (r = 0.995) was observed between the lettuce head index, and leaf index and both had a strong positive correlation (r = 0.828 and 0.760) with NDVI. The highest SPAD values were measured for cos lettuce ‘Romaine lettuce’ (44.01) and iceberg lettuce ‘Great Lakes 659’ (42.71). However, these genotypes showed the highest (9.52%; 8.74%) dry matter content, too. The red leaf variety 'Lollo Rosso' had the highest total polyphenol content (181.53 GAE/100 g FW). Among the evaluated properties, iceberg lettuce showed favorable morphology, plant condition, and good dry matter content. In addition, between the loose-leaf lettuces, the red leaf lollo type was outstanding with bioactive content.
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The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time
27-29.Views:313Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.
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Pomology of gene resources bearing resistance to Erwinia amylovora
29-33.Views:138The ecological farming and an increasing demand for healthy fruits free from chemical residuals necessitated the reevaluation of the existing cultivars and breeding suitable cultivars for the new requirements. We have gathered the old genotypes known in the Carpathian Basin, and we have selected the most suitable ones according to fruit quality and resistance to diseases in the last fifteen years. The main aim was the selection of resistant genotypes against fire blight and powdery mildew as gene sources for our breeding program. On the other hand, we consider the best quality traditional cultivars as extensive landscape elements (wayside tree rows or extensive squash producing plantations).
During the pathological evaluation 'Szemes alma', 'Pónyik', 'Sikulai', 'Tordai piros 'cavil' and 'Szabadkai szercsika' cultivars proved to be good resistance sources (due to their outstanding resistance to fire blight). The latter four cultivars are not susceptible even to fungal diseases, and their quality is also acceptable so those are commendable for ecological farming or for renewing the sparse orchards. According to fruit quality and low susceptibility to diseases 'Batul' and 'Vilmos renet' can also be offered for extensive organic production.
We have made original or renewed pomological descriptions of the selected cultivars, and we also publish their characters according to the terms of UPOV TG/14/8 based on to our evaluations.
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Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods
37-42.Views:203Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.
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An investigation of genotype-environment interaction and stability for pea (Pisum sativum L) seed yield
53-55.Views:116Eighteen pea breeding lines and three check cultivars were tested in two years with and without irrigation. The linear regression can explain only a small part of GEI so the use of regression technique was not possible for interpreting the data. In this case the ecovalence, stability variance and superiority measure stability parameters cannot describe properly the genotype's response. With the AMMI method it is possible to group properly the genotypes according to their response.
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The Hungarian pear germplasm as source of genetic variability for breeding programmes
7-13.Views:272TheHungarian pear collection (Pyrus communis L.) consists of 423 genotypes distributed over seven genebanks inHungary. This is one of themost extensive collections of native and cultivated pears found in Eastern Europe and includes a wide range of genotypes with small size fruit (referred to as “Miniature pears”). Based on the in situ and ex situ measures taken by governmental and other institutions for fruit tree conservation in Hungary, an overview is given on some activities regarding areas of Pyrus collection and genebanks where pears are collected and grown. Descriptions of traits of miniature pears found in Hungarian genebanks for the interest of genetic characterization and breeding are presented.
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Reactions of some cucumber (Cucumis sativus L.) lines and hybrids to zucchini yellow mosaic virus (ZYMV) and selection of tolerant breeding lines
66-68.Views:172In the past years zucchini yellow mosaic virus (ZYMV) has been appeared as a new pathogen of cucurbitaceous plants in Hungary. It caused severe disease outbreaks on the fields of pickling cucumber hybrids which are highly tolerant to cucumber mosaic virus (CMV). Preliminary inoculation tests have showed that all of the important pickling cucumber hybrids produced in Hungary were susceptible to ZYMV-P. However, a selection of Chinese Long and the breeding line I- KS 10C proved to be highly tolerant. The Fl plants of the crosses between the tolerant lines and the susceptible genotype H I were susceptible to ZYMV-P. F2 populations segregated for susceptible and tolerant individuals at a ratio of 3:1. The results strongly suggest that the resistance to ZYMV in our sources is controlled by a single recessive gene. Inoculations of the ZYMV and CMV susceptible cultivar Budai csemege and the CMV tolerant hybrid Perez Fl with the complex of ZYMV+CMV resulted extremely severe symptoms (strong mosaic and necrotic spotting of the leaves) on both cultivars. On the Chinese Long line, which is tolerant to ZYMV and CMV, respectively, the complex of the two viruses caused mild symptoms. The results show a synergistic pathological effect of ZYMV and CMV on differrent cucumber genotypes. More detailed studies on the interactions among the plant genotypes, viruses and virus strains are needed to develop cucumber hybrids that are highly resistant to the ZYMV+CMV complex.