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• Antioxidant capacity and total polyphenolic content in quince (Cydonia oblonga Mill.) fruit

  33-35.

  N. Papp

  T. Szabó

  Z. Szabó

  J. Nyéki

  A. Hegedűs

  Views:

  1261

  Fruits of twelve quince (Cydonia oblonga Mill.) commercial cultivars and selections were compared in the ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) of intact and peeled fruits. The antioxidant capacity and total phenolic content ranged between 5.99 and 63.10 mmol AA/100 g FW, and 3.92 and 12.83 g GA/100 g FW, respectively. These ranges cover an almost 8-times variation among the tested genotypes in case of antioxidant capacity and also declares two-times variation for total polyphenolic content. Cultivars ‘Aromate’ and ‘Bereczki’ possessed the lowest antioxidant capacity and ‘Román portugál’ and ‘Bereczki’ had the lowest polyphenolic contents. In contrast, cultivars ‘Champion’ and ‘Konstantinápolyi’ possessed the highest antioxidant capacity and ‘Mezôtúri’ and ‘De Husi’ had the highest polyphenolic contents. The Pearson’s coeffi cient was relatively moderate but signifi cant (r=0.51) indicating a weak interdependence between FRAP and TPC values of quince fruits. Our results led us to the conclusion that quince might be considered as a valuable source of antioxidants and polyphenolics.

• Security of growing habits and bud formation of German sour cherry varieties

  45-48.

  D. Franjo

  S. Szőke

  J. Nyéki

  P. Hilsendegen

  Views:

  908

  Intensive sour cherry production is concerned to find the most productive varieties under special growing conditions. High planting density, adapted to manual picking or on the other hand to mechanical harvesting. Almost as important is the prolongation of the ripening season by enlarging the choice of varieties. Unfortunately, three of our four leading commercial varieties are ripe almost at the same date.
  Therefore, new varieties ought to be examined thoroughly. In the Pallag Experimental Station of the Debrecen University, five European varieties have been grown (’Schattenmorelle’, ’PI-SA 12,100’, ’Jade’, ’Gerema’, ’Achat’) and a Hungarian one, ’Debreceni bôtermô’ used as a check for the experiments to compare their growing and yielding habits in 2010. The plantation was three years old, standing on Prunus mahaleb rootstocks, in high density (5 x 2 m) and trained to slender spindle crowns. The results are proving that some of the varieties in
  question are suitable to prolong the harvesting season. Growing habits and yields of the varieties related to the variety ’Debreceni bôtermô’ were similar or even better as ’Jade’, ’Gerema’ and ’Achat’. The ’Jade’ excelled with its vitality and ’Gerema’ with its generative character. Further studies are expected to prove the utility of
  one of them at least to enlarge the ripening season on the fruit market.

• Comparative study of cherry varieties used in intense culture

  71-74.

  B. Vaszily

  Views:

  360

  Research in sweet cherry production is intensely stimulated worldwide. The programs started also in Hungary to develop technologies and to find suitable varieties for the purpose of intense cultivation. It means that dimension of crowns should be smaller, with higher number of plants per hectare. Understocks, which let grow the trees slower, are scarce in this species. On the one hand, the braking effect of the respective stocks is insufficient, they get old pretty soon, loose ramification, yield too small fruits and do not comply with the aims of intense cultivation. Experiences prove the necessity of stocks for intense culture, which are vital, growing, easily regenerating, and freely branching. Mahaleb (Prunus mahaleb), a strong growing stock, is still suitable. Further improvement is expected from the contribution of technological elements and the choice of variety. In the present paper, a 9-year old plantation is shown with trees trained to (slender) spindle, and the yield and fruit quality of the year 2009 has been analysed with 6 varieties involved.

• The effect of day and night temperatures on apple skin colour formation

  21-25.

  L. Lakatos

  T. Szabó

  Z. Szabó

  M. Soltész

  S. Zhongfu

  Y. Wang

  J. Nyéki

  Views:

  1425

  The colour of fruits is considered to be an important quality indicator. Saleability greatly depends on how well covered the colour is of the specific type of fruits. It is a well-known phenomenon by growers that apples get nicer colours in one year while in other years the basically red and green colour cultivars can be differentiated only by morphological characteristic features. Cover colour is one of the phenometric variables and it is a well-known fact that significant differences can be experienced year by year. The experienced oscillation can be the cause of inappropriate water- and nutriment supply, however it can be the result of some kind of plant disease, extremely high or low temperature, setting rate above the average and outstanding fruit density. In the present examination it is postulated that the degree of cover colour is mostly influenced by day and night temperature. Therefore, our study aims to find out whether it is true or not. Cover colour belongs to those phenometric characteristic features, only the final value of which is taken into consideration; due to their nature of establishment or forming time it seems useless to follow closely the change in the time of vegetation. However, determining the start of colouring and knowing the dynamics of full colouring could carry very important information for growers. If it is possible to determine the curve describing the time change of colouring, we have a possibility to estimate it by means of enviroment variables. So it is also possible to model pigmentation in the future. Knowing this, colouring irrigation could be made more efficient in the future. For this, as a first step, it is inevitable to find out what the relationship is between the main meteorogical variables, namely day and night temperature and the difference between day and night temperature, and colour cover. In this study we summarize and show these interrelations.

• Effects of weather characteristics on blooming dates in an apple gene bank plantation between 1984 and 2001

  37-44.

  L. Lakatos

  T. Szabó

  J. Racskó

  M. Soltész

  Z. Szabó

  J. Nagy

  J. Nyéki

  Views:

  1183

  The aims of this paper was to investigate the flowering characteristic of apples and their relationship to meteorological parameters. The trees observed are grown at Ujfehert6, Eastern Hungary in the plantation of an assortment (gene bank) with 586 apple varieties. Each of the varieties were observed as for their dates of subsequent phenophases, the beginning of bloom, main bloom and the end of bloom over a period between 1984 and 2001. During this period the meteorological data-base keeps the following variables: daily means of temperature (°C), daily maximum temperature (°C), daily minimum temperature (°C), daily precipitation sums (mm), daily sums of sunny hours, daily means of the differences between the day-time and night-time temperatures (°C), average differences between temperatures of successive daily means (°C). Between the 90th and 147th day of the year over the 18 years of observation. The early blooming varieties start blooming at 10-21 April. The varieties of intermediate bloom start at the interval 20 April to 3 May, whereas the late blooming group start at 2-10 May. Among the meteorological variables of the former autumnal and hibernal periods, the hibernal maxima were the most active factor influencing the start of bloom in the subsequent spring.

• Reactions of different plant organs of pear cultivars to Erwinia amylovora infection

  17-21.

  K. Honty

  Z. Boldog

  M. Göndör

  J. Papp

  K. Kása

  M. Hevesi

  Views:

  360

  Research project has been initiated in 1999 with the aim of evaluating the degree of susceptibility/resistance of pear cultivars grown in Hungary to fire blight disease caused by Erwinia amylovora. The recently selected promising cultivars were also examined. Inoculation experiments were conducted in controlled greenhouse conditions because of quarantine regulations in Hungary. Following the disease process, development of symptoms of plant organs (shoots, flower parts, fruits) was observed. Suspension of two E. amylovora strains (Ea 21, Ea 23) isolated from pear was used in a mixture (5x10⁸ cells x m1^-1) for the inoculation. Twenty-six pear cultivars were examined and grouped into four categories: low susceptibility, moderately susceptible, susceptible and very susceptible. Most of the cultivars were susceptible or very susceptible while some promising 'Eldorado', 'Harrow Delight' and `Hosui' showed low susceptibility.

• Toxicity of fungicides to honey bees (Hymenoptera: Apidae) and their effects on bee foraging behavior, pollen viability and fruit set on blooming apples and pears

  96-98.

  D. R. Mayer

  G. Kovács

  Views:

  342

  Fungicides fosetyl-AL, triadimefon, dodine, mycobutanil and fenarimol were tested for honey bee (Apis mellifera L.) mortality and effect on bee foraging, pollen viability and fruit set in blooming apple and pear. None of the materials were toxic to honey bees or reduced pollen gemination or fruit set.

   

• Antioxidant capacity and total polyphenol content of Lavandula cultivars at different growing areas in Hungary

  65-69.

  E. Détár

  É. Zámboriné Németh

  Zs. Pluhár

  Views:

  637

  Lavandula species are worldwide grown essential oil producing medicinal plants with considerable economic value. Beside volatile oil, lavender also contains different phenolic compounds which have been less widely studied till now. Cultivation of lavender has become widespread in Hungary in the recent years, however, growers have limited knowledge on the productivity of cultivars available. In our research we were aimed to studying the antioxidant capacity and total polyphenol content of samples collected in two growing areas (Dörgicse and Szomód) from flowers of two L. angustifolia (‘Hidcote’, ‘Munstead’) and two L. × intermedia (‘Grosso’, Grappenhall’) varieties, during the full blooming period of 2017. Antioxidant capacity of the samples was determined by FRAP assay (Benzie and Strain, 1996), while total polyphenol content (TPC) was measured by a modified method of Singleton and Rossi (1965). According to our results, varieties of L. × intermedia showed higher values of antioxidant capacity and of total polyphenol content, than those of L. angustifolia cultivars. Among them, both the antioxidant capacity (179.6 mg AAE/g DW) and total polyphenol content values (152.4 mg GAE/g DW) of ’Grosso’ from Dörgicse were the highest. Concerning FRAP values of all the cultivars investigated, larger variability were found in Dörgicse, than in Szomód. Regarding the effect of growing area, each cultivar represented similar FRAP values in Dörgicse and in Szomód, except for ’Grosso’. However, in the case of TPC values, higher variability was observed between the growing areas, especially in the case of ’Grosso’. In the future our studies on lavender polyphenols will be completed with qualitative evaluation of the values obtained by HPLC analysis.

• Innovative research of ornamental plants in University of Debrecen (2001–2014)

  111-117.

  T. Kaprinyák

  Views:

  291

  In the University of Debrecen started in 2001 related and biotechnology, genetic researches on climate-changerelated.
  This work has already visible signs on the city’s public spaces. The main goal of participants of the program is it that
  in Debrecen and in the surrounding communities more adaptable to the variable climate, ecologically and biologically more
  grounded urban ornamental planting are preferred in the next decade. The new results and ongoing researches of the decorative
  ornamental and biomass plants are an interdisciplinary breeding program.

• Peat substitutes in growing cucumber transplants

  115-118.

  N. Kappel

  K. Slezák

  Views:

  315

  The use of an adequate medium is very much emphasised in growing transplants. Due to their favourable characteristics, peats have long been used in production. With the depletion of peat resources the research of peat substitutes has come in the foreground. In the experiments cucumber transplants were grown using baked, expanded clay granules. Results have indicated that by mixing them to peat in a 50 % rate they could be suitable mineral material as a component for soil mixtures.

• Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia

  46-52.

  W. Biratu

  B. Molla

  H. Abebe

  H. Gebremeskel

  Views:

  389

  The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha^-1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.

• Estimating of water consumption of cherry trees

  15-17.

  Á. Juhász

  L. Tőkei

  Z. Nagy

  K. Hrotkó

  Views:

  441

  Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday<sup>-</sup> I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.

• New sour cherry cultivars selected from local sources

  79-80.

  T. Szabó

  F. Szőke

  Views:

  672

  New sour cherry cultivars selected from local sources

• High antioxidant - and anthocyanin contents of sour cherry cultivars may benefit the human health: international and Hungarian achievements on phytochemicals

  45-47.

  Zs. Veres

  I. J. Holb

  I. J. Nyéki

  Z. Szabó

  J. Remenyik

  M. G. Fári

  Views:

  1580

  Evidence suggests that a diet with high fruit and vegetable consumption may decrease the risk of chronic diseases, such as cardiovascular diseases and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proved that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. According to the published data, the most important biological effects of sour cherry are connected — directly or indirectly — to their endogenous antioxidant behaviour as well as to their specific pattern of anthocyanin components. In the present work, we measured the total antioxidant capacity of some Hungarian sour cherry varieties in combination with their anthocyanin-, and vitamin-C content. In 2003, twelve clones were selected and grafted from a local sour cherry population called "Bosnyák" sour cherry grown in small home gardens and farms of the village Csengod (Great-Plain Region, South Hungary). Other Hungarian sour cherry varieties, i.e. cv. Újfehértói fürtos, cv. Érdi bőterrnő, cv. Debreceni bőterrnő, cv. Csengődi and cv. Kántorjánosi served as a control.

• The effect of different carbohydrates on the multiplication of Hosta cultivars

  115-117.

  E. Jámbor-Benczúr

  A. Mándy

  Z. Sinkó

  B. Hipszki

  Zs. Szafián

  J. Dobránszky

  Views:

  457

  The effect of seven concentrations of two carbohydrate sources were compared to determine the best source and the most suitable source and concentration for micropropagation of some Hosta cultivars: H. 'Gold Haze', H. 'Gold Drop' and H. 'Dew Drop'. 0, 5, 10, 20, 30, 40 and 50 g/1 sucrose or glucose were added to a MS basic medium supplemented with 3 mg/1 kinetin and 0.1 mg/1 IAA. For 'Gold Haze' 40 g/1 sucrose proved to be the best source and concentration, the proliferation ratio was 15 shoots per explant. Thirty g/1 sucrose concentration was the optimum for 'Gold Drop', the proliferation rate was 14.6 shoots per explant. In 'Dew Drop,' the best results were obtained with 30 g/1 sucrose but 40 g/l sucrose gave good results too. Both cultivars rooted well on these media. On glucose containing media, very low propagation rates were found in all concentrations and all examined cultivars.

• Sex expression of flowers in cultivated sweet and sour cherries

  50-52.

  D. Surányi

  Views:

  399

  During the period of 1968-1972 nine sweet cherry varieties were investigated by the author on mazzard seedling rootstock, more­over cv. Germersdorfi óriás and cv. Münchebergi korai sweet cherry cultivars grafted on mahaleb, cv. Korponai and cv. Sukorói cherries were observed on rootstocks of cv. Cigánymeggy sour cherry seedling, too.

  Yet, there are other relations between the different parts of sweet cherry flowers too, which perhaps indicate the effect of rootstocks in Cerasus scions. The flower structure shows feminine character on mahaleb, the effect is intermediate on mazzard, however, the sour cherry rootstock strengthens the male character.

  Four sour cherry varieties as cv. Cigánymeggy C. 404. cv. Érdi nagygyümölcsű, cv. Meteor korai and cv. Pándy C. 101 were studied on three rootstocks: mazzard, mahaleb and Cigánymeggy C. 215 seedling rootstocks between 1976 and 1980.

  The pistil length, stamen number and relative stamen number diverged significantly on different rootstocks. The results revealed a close negative correlation between the pistil length and stamen number, furthermore noticing that, in the self-fertile cv. Meteor korai and cv. Újfehértói fürtös presented increasing of the relative stamen number, so the fertility decreased, while in the case of self-sterile varieties the change was favourable.

  Summing up the results, it is obvious, that the fertility of flowers can be modified by rootstocks and the ecological factors cause sex reversions on different combinations.

• Nutritional quality, fruit shape and relationships among exotic and local Capsicum pepper genotypes in Uganda

  33-39.

  S. Asiimwe

  M. Ochwo-Ssemakula

  P. Ssekkadde

  C. Ribeiro

  J. Karungi-Tumutegyereize

  Views:

  567

  Twenty-one hot pepper genotypes comprising of local (15) and exotic (6) types (C. annuum, C. frutescens and C. chinense) were characterized for selected fruit traits after propagation in a glasshouse at the Makerere University Agricultural Research Institute Kabanyolo in Central Uganda using a completely randomized design with three replicates. Ripe fruits were harvested and analyzed; traits evaluated were all significantly different at P<0.05 with variations in quality attributes. The genotype OHA-B305-10 had the highest ascorbic acid content (128.86 mg/100 g) and is recommended for improvement of both local and exotic genotypes targeting the fresh market. Genotypes CAP0408-12 and UG2 WE0511-22, with highest total soluble solids (16.17 ºBrix) and dry matter content (28.59%), respectively should be used in improvements for industrial use or processing to products such as chilli powder or flakes. BRS-M205-04 with highest titratable acidity (1.04%) can be used in enhancing shelf life of genotypes with low titratable acids as well as for the fresh market. In spite of the intraspecific relationships among genotypes, significant differences were observed in their quantitative traits. These genotypes will, therefore, be useful in improving the quality of hot pepper fruit in Uganda.
  
  

• Virus and virus-like diseases of grapevine in Hungary

  25-36.

  J. Lázár

  Gy. D. Bisztray

  Views:

  510

  Viruses and viroids are submicroscopic infectious particles which can cause disease symptoms on grapevine. These parasites are depending completely on the energy metabolism of the plant cell. To enter the host cell plant viruses depend on injuries or on transmission via invertebrates (insects, nematodes, etc.). Viruses are classified by many characters including particle morphology, host range and information content of the genome. At present about 70 viruses including 7 viroids infecting grapevine are known. In single or mixed infections they are potentially detrimental to the quality and quantity of grape production in any growing area of the world. Some viruses can cause severe economic damage in vineyards. In Hungary many important viruses and viroids have been detected in grape. This review summarises characteristics of viruses and the results of detection and characterization of virus and virus like diseases of grapevine in Hungary. The identification of the causal agent, its transmission, geographical distribution and the development of the diagnostic methods are also discussed.

• The effect of potassium fertilization on the inner values of spice pepper

  27-29.

  B. Irinyi

  J. Kapitány

  I. Terbe

  Views:

  386

  To reserve the fertility of soil is the basic condition of successful cultivation. The field specific nutrient supply, based on soil tests is very important in both economy and ecology aspects. Spice pepper plants have high nutrient demands. Proper nutrient supply has importance not only from the point of potential yield levels, but make a major contribution to quality. This is the reason which necessitates the rational development of nutrient supply as an the elements of the production technology. Our objective was to offer a contribution to this job through our nutrient trials. Experiments on the nutrient supply of spice pepper were set up in the 2005 growing season in order to decide whether yields and fruit composition parameters of pepper could be increased by means of increased K fertiliser closes with lower N:K ratios. Several forms of potassium were used, as well as applying microelement top dressings in the single treatments. It was found that higher K doses lead to increased yields, and the influence on dry matter and pigment content proved beneficial too. Potassium applied in the form of patentkali seemed to be better than in that of potassium-sulphate.

• Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers

  39-54.

  T. Bubán

  E. Rutkai

  L. Dorgai

  S. V. Thomson

  Views:

  343

  Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.

  After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the Maryblyt^TM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.

  For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.

  The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.

  We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.

• Microsporogenesis of peach (Prunus persica L. Batsch) varieties

  7-10.

  L. Szalay

  B. Timon

  Z. Szabó

  J. Papp

  Views:

  1275

  Bud dormancy during winter is a critical factor in peach production in Hungary. The yield is determined basically by the survival rate of flower buds during winter frosts and by their ability to develop normal floral organs. It is important to investigate the genetic basis of slow floral development during dormancy for the purpose of breeding peach varieties with better winter hardiness. The aim of the present research was to examine microsporogenesis in 14 peach varieties during three successive winters in a Hungarian germplasm collection and to study the effectiveness of this method in variety evaluation. There were significant differences in the dynamics of microsporogenesis both between the varieties and between the years. Of the varieties, ‘Mayfire', bred in California, possessed the quickest pollen development rate. The microsporogenesis of `Piroska', a Hungarian local variety, was the slowest. Rapid floral bud development was observed in `Aranycsillag', `Springcrest' and 'Venus'. A medium developmental rate was characteristic of `Babygold 6', Fairlane', `Michelini' and `Red June', while development was slow in 'Champion', 'Early Redhaven', `Redhaven', `Harko' and `Mariska'. Based on these results, the study of microsporogenesis represents a reliable method for the phenological description of peach varieties during dormancy. The application of this method makes it possible to identify varieties and landraces with slow flower bud development, suggesting better winter hardiness.

• The estimation of pollen viability of snowdrop (Galanthus nivalis L.)

  141-144.

  G. Kovács

  Views:

  262

  In the present experiment pollen viability of snowdrop were tested using four vital dyes (TTC, Baker' procedure, MTT and FDA) to determine their potential to differentiate fresh living pollen from pollen heated for 12 hours at 80 °C (killed pollen). We found that two of the four dyes previously employed to determine viability also stained killed pollen in the case of snowdrop, while the FDA and MIT did not. We suggest that the two latter are probably the best method to test snowdrop pollen viability, since they do not normally stain either killed or aborted pollen.

   

• Agrobacterium transformation of Rhodiola sp.: current status and limitations

  37-42.

  A. Javid

  Zs. György

  Views:

  411

  The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19^th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.

• Precision geoinformatical system of the pear gene-collection orchard

  43-50.

  J. Tamás

  A. Nagy

  T. Fórián

  J. Nyéki

  T. Szabó

  Z. Szabó

  Views:

  1058

  The principle task of the sustainable development is the preservation of the genetic variety, which is similar challenge in the horticulture regarding the sublimation of fruit species. The breeders of the traditional fruit strains give stock to the sustenance diversity of the agro-environment on the species and landscape level. In 2009, hyperspectral images have been taken by AISA Dual sensors from the pear gene pool in Újfehértó, Hungary. The hyperspectral data cube (in the wavelength range of 400-2500 nm, with 1.5 m ground resolution) ensured possibility to make the spectral library of pear species. In the course of the simultaneously field work the spatial position and individual extent of all pear trees was defined to set up a detailed GIS data base. The water stress sensitivity of single species and the descriptive spectral curves were determined with common evaluation of the spectral and spatial data. Based on the unique methodology processing and the hyperspectral data base suitable strains can be chosen for agro-environment and let take adaptive stocks regarding climate change into the genetic grafting work. Furthermore we could determine and map the sparsely species in the region with the help of the hyperspectral data.

• Bioactive phenols in leaves of Forsythia species

  57-59.

  É. Sedlák

  L. Borsodi

  E. Boldizsár

  É. Preininger

  M. László

  É. Szőke

  I. Gyurján

  Views:

  456

  Nowadays a number of lignans (arctigenin, matairesinol, pinoresinol and phillygenin) have come to the fore in research due to their various biological activities. In this paper the accumulation of these constituents in leaf extracts of Forsythia plants (F. intermedia, F. ovata 'Robusta’ and `Tetragold', F. suspensa, F. viridissima) was quantified using a new isolation method, supercritical CO<sub>2</sub> fluid extraction. The total phenolic and flavonoid contents, the antioxidant capacity and the aglycone lignan profile were determined in leaf extracts of Forsythia species. Within the phenols, the flavonoids were only present in small quantities, but the amount of aglycone lignans was extremely high. F. ovata `Robusta' had the highest total lignan content (103.8 mg/g) of all the Forsythia species. The main lignan in this species is arctigenin, which normally makes up about 60% of the total lignan content, but in the case of F. ovata `Robusta' this value was 96.1%. Since this arctigenin content is outstanding compared to that of other Forsythia species, it could be promising to develop a fermentation technology for the production of this natural compound.