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Development of different herbaceous perennial species on the experimental extensive green roof of Corvinus University Budapest
85-89.Views:123During 2003-2004 six "traditional" herbaceous plant species (Sedum reflexum, Sedum acre 'Aureum', Sedun spurium„Sedum floriferum, Festuca glauca) and six "new" plant species (Sedum pulchellum, Prunus tenella, fivericum polyphyllum, CeraiTtignIct plumbaginoides, Dianthus plumarius and Phlox subulala) were tried on four different types of roof insulation on the 100 m large experimental extensive green roof of the Building K of Corvinus University Budapest, at Villányi street, Budapest. According to the results obtained so far, the viability and good decoration values of all the "traditional" plants were proved. From the six "new" plants Ceratostigma plumbaginoides, Dianthus plumarius and Sedum pulchellum (with some restrictions) proved to be suitable. A significantly poorer development was showed by the remaining "new" species (Phlox subulata, 1-1yericum polyphyllum, Prunus tenella) than the "traditional" ones. As for the different types of roof insulation, the best was with the direct order of layers, and drainage layer with water storage capacity.
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Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods
37-42.Views:203Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.
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Preliminary results on salicylic acid treatment on brown rot caused by Monilinia laxa on Jumbo Cot fruit, Prunus armeniaca L.
37-39.Views:381The effect of salicylic acid on reducing brown rot caused by Monilinia laxa infection in postharvest apricot fruit Prunus armeniaca L. Freshly harvest Jumpo Cot fruit were treated with water as control treatment and other group treated with salicylic acid 2 Mm as induced resistance treatment, Fruits were infected by M. laxa (1 × 10-3spores ml-1) and incubated at 25 oC for 2, 4 and 6 days. Treatment fruits with salicylic acid resulted in direct effect on mycelial growth as in the salicylic acid treatment the growth reached to 45% after 6 days of incubation while in the control treatment it reached to 100%.
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Antioxidant characterization of apricot (Prunus armeniaca L.) cultivars and hybrids
47-51.Views:227This report forms a part in our long-term study dedicated to reveal the antioxidant properties of apricot fruits through several years. Nineteen apricot cultivars and 8 hybrids were comparatively analysed. Total phenol content and antioxidant activity showed a good correlation. The tested cultivars and hybrids could be arranged in three groups representing different antioxidant activities, 12 entries were involved in the group with relatively low antioxidant capacity (FRAP value < 1500 mmol/L); 10 accessions were classified in the group with medium antioxidant capacity (1500 mmol/L < FRAP value < 3000 mmol/L); and 5 genotypes were grouped to the category of high antioxidant value (FRAP > 3000 mmol/L). `Morden 604' produced a surpassingly outstanding antioxidant character. H-donating ability has further supported our results. Phenolic substances were accumulated and ferric reducing ability was increased in the ripe fruits compared to the unripe ones. High levels of variations in the total phenol content and antioxidant capacity in of apricot fruits were revealed in this study. Environment, year or rootstocks may also influence the antioxidant properties of fruits. however it seems to be convincing that crossing parents with outstanding antioxidant character can produce hybrids with higher antioxidant capacity. Hungarian apricots are widely appreciated for their premium flavour and — as it was evidenced in this study — for their relatively good antioxidant properties. However, delicious fruits should be made functional foods having beneficial health effects through accumulating large amounts of antioxidant molecules in the fruit flesh.
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Comparative analysis of sour cherry cultivars on their ecological and biological indicators
7-28.Views:361Sour cherries developed in the northern hemisphere, an alloploid hybrid of dwarf sour cherries (Prunus fruticosa) and bird cherries (P. avium), born in the confluence of the two species. However, the ecological and, above all, cold tolerance of the ancestor of cultivated sour cherries is higher than that of wild cherries (De Candolle, 1894; Rehder, 1954; Terpó, 1974; Iezzoni et al., 1991; Faust & Surányi, 1997). The cultivation limits are in the northern hemisphere 38-44. degree. The Carpathian Basin, the Balkans and Asia Minor are considered to be the main birthplaces for sour cherries. The genetic and morphological diversity of sour cherries is greater than that of the basic species (Iezzoni et al. 1991; Faust & Surányi, 1997). In the study, 472 sour cherry cultivars were compared based on 7 relative ecological indicators and 3 biological values. Compared to other Prunus species, we mostly found less variability in sour cherries - not counting their salt tolerance (SB). The partial similarity between open pollination (OP), frost tolerance (FR) and disease resistance (DR) - partly true in terms of varieties, but also reflected the effects of purposeful breeding and selection. The cultivars together - in comparison, showed balance, but in the highlighting, the differences of the 3 cultivar groups became significant. Indeed, the differences between the species of the former Hungarian cultural flora are clearly different (Surányi, 2004), which is also the case when comparing a large number of apricot (Surányi, 2014), plum (Surányi, 2015) and peach (Surányi, 2020) varieties.
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Determination of auxine content of soft wood cutted `Marianna GF8/1' (Prunus cerasifera x P. munsoniana) by High Performance Liquid Chromatography during rooting period
67-70.Views:138The content of different auxins of soft-wood cutted plum rootstock 'Marianna GF8/1' (Prunus cerasifera x P. munsoniana) was determined during the rooting period. The level of auxin-concentration (exogenous and endogenous) of basic and intemodal part of cuttings was determined by WATERS HPLC equipment every 7 days during rooting period. The lengths soft wood cuttings were app. 30 cm long. The basal part of shoots were treated with 2000 pg/g concentrated indole-butiryc acid in talcum powder. After treatment the cuttings were placed in propagation green-house under intermittent mist. The plant hormones were extracted by methanol the solution was cleaned by paper-filter, and further cleaned by centrifuge. The effluent was examined by reversed phase High Performance Liquid Chromatography, with WATERS 2487 dual detector at 220 am on Symmetry C18 4,6x 150 column. Recovery and reproducibility assessment indicates good accuracy and acceptable relative standard deviation (RSD) 5%. Linear responses (r20.997) for calibration curve was obtained with IAA, IPA and IBA standard in range, with a limit of quantification of 0.15 g•m1-1. The concentration of IAA, IPA and IBA in the basal part of cuttings were measured, during the rooting period. We proved the external IBA was taken up by the plants. In the plants were found the IBA, and the IAA concentration of IBA treated cuttings was higher, than the untreated one.
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Determination of chlorophyll content in case of peach leaf curl disease (Taphrina deformans) with spectral analysis
49-52.Views:372One of the most important conditions of high quality fruit production is the early detection of hytopathologycal infections. The most dangerous disease of peach (Prunus persica) is the Taphrina deformans, which causes serious damages mainly in the years where the weather condition is cool and wet. In this study, healthy and naturally diseased leaves by peach leaf curl were investigated in the laboratory of University of Debrecen, CAAES, Institute of Water and Environmental Management. Both of hyperspectral measurement and the values of spectrophotometer shown the chlorophyll content of diseased leaves were lower than the healthy samples. Based on the experiments the water status of investigated leaves established the infected leaves contained more water in their tissues.
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Determination of the cold tolerance off sour cherry cultivars with frost treatments in climatic chamber
49-54.Views:267Nowadays, sour cherry buds can be seriously damaged by spring and winter frosts. Unlike other fruit species threatened by high frost damage, sour cherry cultivars have not been assessed for frost tolerance. The aim of .our survey was to establish the relative cold tolerance of the Hungarian cultivars after treatment in a climatic chamber, and to optimize the methodology formerly elaborated for the frost treatment of apricot. Fourteen cultivars of Hungarian sour cherry (Prunus cerasus) were used in the experiments, which spanned the winters of 2005/2006 and 2006/2007. Our data were used to rank cultivars in two groups according to their levels of cold resistance. We also recommend critical temperatures and treatment times for the testing of sour cherry cultivar resistance to cold in climatic chambers.
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Self-incompatibility alleles in Esatern European and Asian almond (Prunus dulcis) genotypes: a preliminary study
23-26.Views:210Almond [Prunus dulcis (Mill.) D. A. Webb.] as one of the oldest domesticated plants is thought to have originated in central Asia. Gametophytic self-incompatibility of almond is controlled by the highly polymorphic S-locus. The S-locus encodes for an S-ribonuclease (S-RNase) protein in the pistils, which degrades RNA in self-pollen tubes and hence stops their growing. This study was carried out to detect S-RNase allelic variants in Hungarian and Eastern European almond cultivars and Turkish wild growing seedlings, and characterize their S-allele pool. Five new alleles were identifi ed, S31H, S36-S39 in Eastern European local cultivars. The village Bademli and Akdamar island are two distinct places of almond natural occurrence in Turkey. Trees growing wild around Bademli city showed greater genetic diversity than those originated on Akdamar island. Many of the previously described 45 S-RNase alleles have been also detected in these regions. Homology searches revealed that Turkish almonds carried some P. webbii alleles indicating hybridization between the two cultivars and massive introgression events. Our results supply long-awaited information on almond S-allele diversity from regions between the main cultivation centres and the centre of origin of this species; and are discussed from the aspect of methodological developments and evolution of the cultivated almond.
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Changing of inert content values of apricot varieties
39-41.Views:199The consumers have been exigent increasingly about excellent quality. The speci fie flavour and fragrance of apricot evolve during ripening. We need to be acquainted with changing of inert content during ripening to the choosing of optimal harvest time. The Regional University Knowled ge Centre -Research and Development in Foodstuff Chain project had presented an opportun ity to study the inner content of apricot. Our aim was to study evaluation of health care attributes of apricot fruit. We investigated different ripeness samples of four apricot varieties ('Mandulakajszi', 'Pann 6nia', 'Gonci magyar kajszi'. 'Harcot') in our study of 2007-2009. Samples were taken the orchard of Sóskútfruct Ltd. Hungary. We had measured the water soluble antioxidant capacity, polyphenol-, f3-carotene content and the dissimilar sugar and acid-fractions during ripening. We had experienced significant different in inert content between varieties.
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Long term investigations of flowers and leaves on mainly non-domestica plums
73-79.Views:190The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings of C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification.
The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data.
Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).
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Inheritance of male sterility in apricot
12-14.Views:138Progenies (total of 1,114 seedlings) from crosses representing all possible genotypic combinations between 4 male-fertile and 1 male-sterile apricot parents were scored for the male sterility trait. Crosses between putative heterozygous normal cultivars yielded 25% of male-sterile seedlings, which supports a previous hypothesis that male sterility is controlled by a recessive allele of one nuclear locus. Crosses between those parents and putative homozygous normal cultivars did not produce any male-sterile tree. Finally, the proportion of male-sterile progeny in crosses between a male-sterile and two male-fertile cultivars depended on the genotype of the male parent. When it was heterozygous approximately 50% of the progeny was sterile, whereas when a homozygous fertile parent was used, no male-sterile progeny was obtained. These results confirm a previously proposed model, in which the male sterility trait in apricot is controlled by a single recessive gene.
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Recent findings of the tree fruit self-incompatibility studies
7-15.Views:229This review endeavours to collect all recent and substantial contributions to the quickly deepening fields of tree fruit self-incompatibility studies and hence updating previously published reviews. Studies carried out to discover the molecular basis of gametophytic self-incompatibility are summarized and a newly described model for the solanaceous plants is also outlined. We describe recent findings in all economically important fruit tree crops involving apple, European pear, sweet and sour cherries, almond, Japanese plum, sloe, Japanese apricot, European apricot and peach. Additional DNA sequences are now available for both the pistil and pollen component genes in several species and their molecular, evolutionary or economic implications are discussed in the light of the fruit setting behaviour.
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Comparison of varying pollen source on productivity of sour cherry (Prunus cerasus L.) cultivars
23-26.Views:245Fruit set of five sour cherry cultivars (‘Újfehértói fürtös’, ‘Éva’, ‘Petri’, ‘Pándy 279‘ and ‘Csengôdi’) of eight years-old trees grown in Újfehértó, located in the Eastern north part of Hungary have been studied over two years (2008 & 2009). Following reciprocally cross-pollination, free-pollination, self-pollination (autogamy) artificial self-pollination (geitonogamy) were studied. The results show that both maternal and paternal parent cultivates had significant effects on the percentage fruit set. Significant differences have been found in fruit set among years and among pollination treatments. Fruit set of free-pollinated Pándy 276 cultivar was low and seasonally highly variable. The yield of this cultivar on self-pollinated flowers was nearly 0%, and in this treatment the maximum yields did not reach 10% in any of the examined cultivars. There was no significant relationship in the fruit set of free-pollination and natural self-pollination treatments.
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Erratic fruit set in almond under warm climates
59-64.Views:300The cause for the erratic yields of the two main almond cultivars grown in Morocco has been searched in order to recommend possible solutions for a high and stable production. The lack of sufficient bloom overlap between ‘Marcona’ and ‘Fournat de Brézenaud’ in many years may be one of the reasons of this erratic behavior, but not the only one, as the same behaviour may be observed in two simultaneously blooming cultivars, ‘Ferragnès’ and ‘Ferraduel’. The relatively high temperatures observed during the blooming period
significantly reduced the stigma receptivity of these cultivars and, as a consequence, their effective pollination period, showing that stigmatic receptivity is a limiting factor for fruit set in ‘Marcona’ and ‘Ferragnès’ and their subsequent yield reduction under warm conditions. Thus, in the present context of global warming, the search for cultivars tolerant to heat stress during flowering will acquire a special interest, as well as the combination of cultivars with the same chilling and heat requirements to ensure a simultaneous bloom. -
Preliminary evaluation of breeding perspectives of Ukrainian sweet cherry cultivars: nutraceutical properties and self-incompatibility
7-11.Views:357Some traditional sweet cherry cultivars of Ukrainian origin may represent perspective material for Hungarian cherry breeding. A total of eight cultivars analysed represent great diversity in several phenotypic traits including fruit ripening time or fruit flesh colour. Considerable differences in the anthocyanin content may result in different antioxidant capacity of fruits. In the present study, we used ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) assays to characterize fruits’ nutraceutical properties. These values were compared with the respective values measured for eight commercial cultivars grown in Hungary. The average of FRAP and TPC values was higher for the Ukrainian cherries compared with commercial cultivars suggesting they might be included in functional breeding programs. Since, cherry is a self-incompatible species, the determination of S-genotype is required for both breeding and successful cultivar association in commercial orchards. Complete or partial S-genotypes were determined for 5 and 3 cultivars, respectively.
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Vegetative parameters of sweet cherry (Cerasus avium L.) cultivars in two training systems
42-45.Views:339Hungarian sweet cherry production has been changed recently. Thanks to the favourable selling prices many new plantations were established in the last years. In the new orchards mainly spindle canopy are trained, although many type of crown forms are used all over the world. In our study slender spindle and bush canopy were evaluated with three cultivars (‘Petrus’, ‘Vera’, ‘Carmen’) grafted on Prunus mahaleb rootstock. Vigor of the 3-4 years old trees showed spectacular differences. The highest vigor with Spanish bush was found for cv. ‘Vera’, but on slender spindle canopy the thickness values were medium. Meanwhile cv. ‘Petrus’ showed the highest trunk thickness with spindle canopy, but the lowest with Spanish bush. On spindle canopy cv. ‘Carmen’ presented the highest ramification ability, but its 2-3 years old twigs started to bald very early, which can be considered an unfavorable phenomenon. Bush canopy still can be described with strong vegetative growth on 3-4 years old trees, as vigor of spindle trees are more moderate.
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Seasonality of weather and phenology of reproductive organs of flower of sour cherry cultivars in Hungarian climatic conditions
75-80.Views:249Sour cherry (Prunus cerasus L.) is one of the most important fruit crop grown in Hungary, The flowering phenology and pollen shedding/stigma viability ratio, in an effort to elucidate the reproductive phenology of nine economically important sour cherry cultivars (Érdi bőtermő, Debreceni bőtermő, Csengődi, Kántorjánosi 3. Pándy 279. Úfehértói fürtös, Petri, Éva, and Oblacsinszka) all widespread in the Hungary, was studied at Újfehertó climatic condition. The main aims were to investigate how possible environmental cues influence timing and development of phenophases and whether different cultivars have different seasonal responses to these cues. Likely effects of climatic changes on phenological development patterns were also considered. Our results revealed important aspects of the reproductive biology of sour cherry flowers. The amplitude of the phenophase "beginning of blossoming" between cultivars did not exceed 6 days. There is maximum 3 days difference in blooming length of different direction in each cultivar. Pándy 279 showed high variability when the position of flowers changed on the tree shoots. Újfehértói fürtös was stable in all four directions of tree. The pollen shedding period and stigma viability ratio was well synchronous. Pollen shedding phenomenon occurred in the range limitation of secretary activity of stigmas in all five cultivars. Distribution of pollen shedding over the secretary activity of stigmas is almost good. In all five cultivars maximum pollen shedding occurred about the high temperature part of the day.
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The effect of rootstock on the tree size of apricot cultivars
57-58.Views:143The apricot is propagated on several kinds of rootstocks in Hungary. The main aspects of selecting rootstocks are as follows: adaptability to environmental circumstances, primarily soil conditions, ensuring the tree size that complies with the cultivation method, and compatibility with the grafted cultivar. At advanced, intensive orchards rootstocks ensuring smaller tree size are privileged. For the establishment of the appropriate cultivation system, it is important to be aware of the expected growing vigour and tree size of certain cultivarrootstock combinations when the orchard is designed. In the course of our experiment the size of 15-year-old trees of 4 apricot cultivars were examined on several rootstocks at an orchard in Siófok. On the basis of the data measured for each cultivar-rootstock combination, it can be stated that trees on wild apricot (P. armeniaca) rootstocks are the largest in size. Trees on prune (P. domestica) rootstock have 10-15% smaller crown volume than the previous combination. Trees on bullace (P. insititia) rootstock have the smallest tree size and their crown volume is 30-50% smaller than that of the trees on P. armeniaca rootstock. Thus, the application of prune and bullace rootstocks is beneficial at intensive apricot orchards as the size of trees can be reduced by their usage. However, their compatibility with the cultivars and their adaptability to the ecological conditions of the production site have to be tested before applying them widely. In the course of our research incompatibility was not experienced for any of the cultivar-rootstock combinations examined. Nevertheless, the drought tolerance of the rootstocks examined showed significant differences. Trees on P. domestica or P. insititia rootstock requires more water than those on P. armeniaca rootstock, therefore, they have to be irrigated.
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Levels of some micronutrient in dried and fresh fruit samples of apricot cultivars
25-30.Views:511Concentration of Boron (B), Copper (Cu), Iron (Fe), Magnesium (Mg), Manganese (Mn) and Zinc (Zn) was analyzed in fresh and dried fruit samples of “Jumbo cot“, “Tom cot“, “Gold strike“, “Gold bar“, “Bergeron“, “Bergrouge“, “Sweet cot“, “Yellow cot“ and “Zebra“ apricot cultivars. Concentration of the studied elements was strongly affected by cultivars. B, Cu, Fe, Mn and Zn content of “Tom cot“ was significantly higher than other cultivars. “Gold strike“ had the highest amount of Mg. Similar tendency was observed in “Zebra“ and “Sweet cot“ where Mn content was significantly higher than the other element contents.
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Morphological examination of Hungarian apricot rootstock varieties
59-63.Views:272Apricot seedlings are one of the most widely used rootstocks in apricot growing of Hungary. The National List of Hungary contains 7 apricot seedling rootstocks varieties. The production of apricot graft on seedling rootstock takes 3 years from the seed production to the complete graft and requires more space. It is important to provide the trueness to variety name during this process. In the genus Prunoideae not only the plant but also the stone should have typical characteristics. It seems to be useful to examine the morphology of stone. The observed varieties were 'Tengeribarack C.1300', 'Tengeribarack C.1301', 'Tengeribarack C.1650', 'Tengeribarack' C.1652', 'Tengeribarack C.145', 'Tengeribarack C.1426' and 'Tengeribarack C.2546`.1t seems to be the most suitable characteristic to make a distinction between these varieties by stone, the ratio height of stone to lateral width of stone, the ratio of height of stone to ventral width of stone, the ratio of lateral width of stone to ventral width of stone and the ratio of ventral zone to ventral width. From not measured characteristic seems to be most utilizable to make distinction: the fusion of dorsal groove margins, the shape of apex, the presence of a mucro of the apex, the shape of stalk end in lateral view and the texture of lateral surfaces.
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Comparative study of cherry varieties used in intense culture
71-74.Views:191Research in sweet cherry production is intensely stimulated worldwide. The programs started also in Hungary to develop technologies and to find suitable varieties for the purpose of intense cultivation. It means that dimension of crowns should be smaller, with higher number of plants per hectare. Understocks, which let grow the trees slower, are scarce in this species. On the one hand, the braking effect of the respective stocks is insufficient, they get old pretty soon, loose ramification, yield too small fruits and do not comply with the aims of intense cultivation. Experiences prove the necessity of stocks for intense culture, which are vital, growing, easily regenerating, and freely branching. Mahaleb (Prunus mahaleb), a strong growing stock, is still suitable. Further improvement is expected from the contribution of technological elements and the choice of variety. In the present paper, a 9-year old plantation is shown with trees trained to (slender) spindle, and the yield and fruit quality of the year 2009 has been analysed with 6 varieties involved.
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Pollen tube growth in sweet cherry (Prunus avium L.) styles following fully compatible, half compatible and incompatible pollinations
63-68.Views:145In vivo as well as in vitro pollen tube growth studies along the style were performed, each with two pairs of sweet cherry cultivar combinations by means of fluorescence microscopy. In vivo studies showed that the percentage of pollen tubes penetrating the middle and basal section of the style was higher in the fully compatible 'Margit' x 'Alex' combination than in the half compatible `Germersdorfi 3' x `Alex' cross. The year effect was significant at P=0.] probability level. All pollen tubes in vitro stopped at the upper third of the style in the incompatible 'Vera' x 'Van' cross, whereas in the half compatible 'Alex' x 'Van' 50% of the pollen tubes penetrated to the lower third of the style. By in vitro fluorescence microscopy, it was possible to distinguish half compatible combinations from incompatible ones. Results obtained by in vivo technique only were much ambiguous.
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Cracking susceptibility of sour cherry (Prunus cerasus L.) in Hungary and relation to calcium application
109-118.Views:321The rain induced fruit cracking is a big, serious problem especially for sour cherry growers but in some year sour cherry growers had also problem with fruit cracking caused by too much rainfall in the harvesting season. The cracked sour cherry fruits can be easily infected by different diseases like Monillinia sp. Cracked and infected fruits can not be transported for long distance and using for preservation because they lost their market value by the pour fruit quality. There are two possibilities to protect fruits against the rain induced fruit cracking. The most effective protection technique is the plastic rain cover over the tree rows. The installation of these equipments is too expensive for the growers. That is the reason why researchers tried to find other less expensive and sufficiently effective ways like sprayings different mineral salts, hormone and other type chemicals against the rain induced fruit cracking. Several calcium formulas calcium chloride (CaC12), calcium hydroxide (Ca (OH),) and calcium nitrate (Ca(NO3)2) can be sprayed in appropriate solution concentration.
The aim of our trial is to determinate the fruit cracking susceptibility of wide grown Hungarian sour cherry varieties and find the most effective calcium formula and its concentration for spraying in orchards to prevent the fruit cracking. In the first trial year (2006) cracking index of tested sour cherry varieties were determined under field and laboratory conditions. Under field conditions were not found differences between cracking tendency of tested cultivars. After results of immersing fruits in distillated water for 24 hours tested sour cherry varieties were divided to three groups by the susceptibility to rain induced fruit cracking: very susceptible (`Maliga emléke', 'Piramis', 'Érdi jubileum', 'Erdi nagygyümölcsű’ and 'Meteor korai'); susceptible ‘Érdi bőtermő, Tandy 279' and Cigány 59.; moderately susceptible/tolerant ("T" and "R" clones). In the second trial year (2007) calcium chloride (CaCl2) and calcium nitrate (Ca(NO3)2) were sprayed in 0,35m/m%; 0,5m/m% and 1,0m/m% solution concentration. One more commercial product "Damisol-Kalcium" was also applied in the advised 1,0m/m% concentration. Like in the trial year before (2006) under field conditions we did not kept differences between the cracking susceptibility of varieties and calcium treatments. As the result of laboratory testing (immersing calcium treated fruits in distillated water) we kept that calcium chloride (CaCl2) seems the most effective against the fruit cracking in 0,5m/in% solution concentration. The other calcium formulas also decreased the cracking ratio but in less scale.
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Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods
35-41.Views:170Symptoms resembling phytoplasma disease have been observed on peach trees in a seed-source plantation of stone fruits in south Hungary quite recently. In this publication we report on the results of woody indexing of symptomatic peach trees on GF 305 indicator in the field and under greenhouse conditions as well as on molecular studies. Phytoplasma infection detected on GF 305 indicators in greenhouse and field indexing was confirmed by PCR. Nested PCR was conducted using universal primer pairs followed by group and subgroup specific primers for the second amplification. RFLP analysis of nested PCR products was performed using Rsal restriction enzyme. Based on the results of molecular studies it can be concluded that phytoplasmas, belonging to the European stone fruit yellows subgroup (16SrX-B) were identified in peach trees. Further studies on symptomatic peach trees originating from different parts of Hungary are in progress.