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  • Variation between some apricot varieties in regard to flowering phenology in Boldogkôváralja, Hungary

    The aim of this study was the estimation of blossoming of 14 apricot cultivars in Boldogkôváralja in 2009, 2010 and 2011 seasons. And this will help growers to select appropriate varieties to their weather conditions. For this target the blooming period of 19 apricot varieties of different origin was observed in three subsequent years. There was no large difference in the beginning of blooming in the different years, and the greatest variation between the start date of flowering was about 1 to 3 days as the place of experiment site near to northern border and also, length of flowering period of apricot trees is also inversely related to date when blooming started. The little differences in flowering dates and flowering periods due to the high temperature through the three seasons of study.

  • Study on the mushroom species Agaricus blazei (Murill)

    Nowadays, Agaricus blazei is known all over the world as a mushroom species with unparalleled medicinal properties besides its culinary delights and therefore its regular consumption is recommended in the first place for the prevention of various cancers. For the past decade researchers have been active in the study of its therapeutic properties. Its production started only a decade ago and there are a number of questions yet to be answered. At the moment, the most widespread technique is to grow it using composts similar to those of the button mushroom. In contrast to the common button mushroom, its production differs markedly from that of the button mushroom in that it develops fruiting bodies only in light and requires that the compost temperature be high, around 25 °C, also in the fruiting period and has a much higher need for fresh air. It is also sold in fresh form but mainly as a dried product, in powdered form or as capsules or pills.

  • Maceration affects mineral composition and pH of wines

    Mineral composition of wines is affected by many ecological and technological factors. These variables are mostly discussed focusing on certification of origin and human health. This paper gives data on possible changes in mineral composition and pH of wines due to 4 hours skin maceration (1st trial) and fermentation sur marc (2nd trial). Experimental site is situated on acidic sandy soil in middle east Hungary. The variety collection was established in 2003 with own rooted planting material in 3x1 m spacing, trained for single curtain training
    system. Mineral composition of wines was determined with ThermoFischer Scientific iCAP 6300 ICP-OES, pH was measured with pH10pen (VWR International) in field, and with SevenEasyTM pH meter (Mettler Toledo) in laboratory. 1st trial with 9 PIWI white wine grape varieties pointed to demonstrate effects of skin maceration, which is abundantly used to elevate aroma content. 2nd trial with the use of ‘Medina’ red PIWI grape variety aimed to demonstrate effects of double pasta skin maceration in rose and red wine technology. Data of 1st trial show, that K, Cu, Mn and P respective increase with skin maceration, despite Fe show considerable decrease. Data of 2nd trial show, that K, Mg, Mn, P, Sr and B increase with longer skin contact and higher fermentation temperature, despite Fe and Ba decrease with this technology. In regard to pH, data show, that skin maceration and fermentation sur marc increase K content with about 30-70% respectively, withstanding that pH also increase with a considerable 0,4-0,5 value. K content can be higher in the end product, despite to earlier higher level of tartrate formation. An attention should be driven to lower level of tartaric acid and consequently higher pH characteristic for wines produced with the application of skin maceration or fermentation sur marc.

  • Detemination of the time of pruning regarding the ability of developing flower buds and their frost tolerance in sweet cherry varieties

    In sweet cherry growing, intense technology is introduced as the up to date development. Among the elements of the technology are the choice of variety, planting design and pruning are the main elements. The methods, intensity and the timing of pruning are largely dependent from the growing habits of the varieties, the light demand of the parts of different age and their tendency of getting bald on the aging zones. One of the main limiting factors of production, the frost damages are outstanding. In choosing the site of plantation, we must avoid the places, where spring frosts used to occur, the next moment is the role of the variety and the technology applied. In Hungary, the late or spring frosts are considered, however, the winter frosts may also cause heavy losses, which depend also on the health and nutritional condition of the trees when being exposed to temperature minima. Our present study is based on a 9-year-old plantation trained to slender spindle as well as to free spindle crown. We observed the development of fruiting structures and their frost damage also in relation with the timing of the pruning operations during the winter or summer, and compared also the varieties with each other.

  • Simultaneousness of reproductive organs of sour cherry cultivars adaptable to Iranian climatic condition

    Observations were made on simultaneousness of pollen shedding and stigma viability ratio, to clarify the phenology of reproductive organs of nine economically important sour cherry (Prunus cerasus L.) cultivars (‘Érdi bôtermô‘, ‘Debreceni bőtermő‘, ‘Csengődi‘, ‘Kántorjánosi 3‘, ‘Újfehértói fürtös‘, ‘Petri‘, ‘Eva‘, ‘Oblascinszka‘, and ‘Pándy 279’). The main aim was investigation the possible influence of environmental cues in timing and development of phenophases and also responses of different cultivars to seasonal cues. Likely effects of climatic changes on phenological development patterns were also considered. Results showed important aspects of the reproductive biology of sour cherry flowers. The amplitude of the phenophase “beginning of blossoming” between cultivars did not exceed 6 days. There is a maximum 3 day difference in the blooming length of different directions in each cultivar. ‘Pándy 279‘ showed high variability when the position of flowers changed on the tree shoots. ‘Újfehértói fürtös‘ was stable in all four directions of the tree. The pollen shedding period and stigma viability ratio were synchronous. Pollen shedding phenomenon simultaneously occurred with the secretary activities of stigmas in all examined cultivars. Distribution of pollen shedding over the secretary activity of stigmas is almost good. In all five cultivars maximum pollen shedding occurred when the temperature was high during the day. In general the results of this experiment and our previous studies clearly demonstrate that the Pándy groups not recommended for plantation in new orchards of Iran.

  • General defense system in the plant kingdom

    The goal of plant breeders is to improve the resistance of crops against virus, bacterium and fungus pathogens was easiest to achieve by selection for phenotypes displaying the hypersensitive reaction. The resistant plant of that type keeps its health by preventing or delaying the systemization of the pathogen by destruction of cells and tissues of variable size or amputation of the contaminated organs. The faster the reaction of the host plant is the more efficient and economical is the defense, since the extent of tissue destruction decreases proportionally with the speed of reaction.

    During a breeding program for resistance carried out on several plant species, mainly vegetables over thirty years, also an alternative defense reaction has been experienced, which fundamentally differs from the hypersensitive reaction. In that reaction the cells and tissues of the host plant being exposed to the pathogen do not die, on the contrary they hinder systemization of the pathogen by tissue thickening. An additional significant difference is that on the contrary to hypersensitive reaction this reaction is less host- or pathogen-specific and works excellently even at high temperature (over 40 °C).

  • Yield and composition of supercritical fluid extracts of different Lamiaceae herbs

    In our work the effectiveness of supercritical carbon dioxide extraction was studied on volatile components of Lamiaceae herbs. The aim of our investigations was to determine the optimal extraction parameters yielding high amount of volatiles in a desirable composition. As plant materials, dried and powdered cude drugs of Thymus vulgaris, Thymus pannonicus, Satureja hortensis, Ocimum basilicum, Rosmarinus officnalis and Salvia officinalis were chosen. Pressure (8-30 MPa), temperature (35-60 °C) and time (5-60 min) of extraction were regulated. Results obtained by supercritical fluid extraction (SFE) were compared to that of the conventional extraction procedur, hydrodistillation (HD). In the case of Thymus vulgaris, Thymus pannonicus and Salvia officnalis, extract yield of SFE was comparable to the essential oil amount obtained by hydrodistillation from the same drug. Essential oil rich thyme extracts were analysed by GC-FID. We have found that yield and quality of SFE extracts highly depend on the conditions of extraction.

  • Spore dispersal, diurnal pattern and viability of Monilinia spp. conidia and the relationship with weather components in an organic apple orchard

    In a two-year Hungarian study, spore dispersal diurnal periodicity and viability of Monilinia spp. and their relation to weather components were determined in an organic apple orchard. Conidia of Monilinia spp. were first trapped in late May in both years. Low number of conidia were trapped until end-June. Thereafter, number of conidia continuously increased until harvest. Conidia in a 24-h period showed diurnal periodicity pattern, with th highest concentration in the afternoon hours. Spore viability with FDA staining showed that viability of
    conidia ranged from 45 to 70% with showing lower viability in the dry than in the wet days in both years. Temperature and relative humidity correlated positively with mean hourly conidia numbers in both years. Mean hourly rainfall was negatively but poorly correlated with conidiacatches in both years. Results were compared and discussed with previous observations.

  • Visualization of the Gas Chromatography/Mass Spectrometry Data of Muscat Ottonel Must and Wine Measurements

    The lack of interpretation methods useful in evaluating the aroma-profiles of wines makes it necessary to thoroughly investigate alternative evaluation procedures. By adding three appropriate normal hydrocarbon standards to all sample extracts, measuring the Programmed Temperature Retention Indexes of the components and by normalizing the peak areas to that of the 1-alpha-Terpineol (in musts) or Benzeneethanol (in wines), the aroma features could be visualized. The relationship or identity of the aroma patterns could be deduced from the presence or absence of similar polygons in the -constellation-map" of the components.


  • Postharvest methyl jasmonic acid and hot water can reduce the internal breakdown and quality loss of apricot fruit at shelf life

    The aim of this study was to investigate the postharvest effect of methyl jasmonic acid (MJ) and hot water on internal break-down and quality loss of apricot fruit under shelf life conditions. Cultivar Flavor cot apricot fruit were used to treat with water as control treatment, with 0.2 mmol/L MJ and with hot water 35 oC for 5 min. Fruit were stored at room temperature and were examined every 2 days for internal break-down and quality loss. Results showed that treated fruits with MJ and hot water showed the lowest weight loss and the highest firmness during all assessment times. Control fruits showed losing of customer acceptance from the day 2 of shelf life and then decreased dramatically to approximately loss all the acceptance at day 8. The SSC showed sever reduction in untreated fruit after day 6 at shelf life. Total phenol content reduced and polyphenol oxidase (PPO) increased in all assessment times for all treatments. Meanwhile MJ showed the best values for phenol content and lowest PPO activity. The results supported the idea of using some elicitors like methyl jasmonic and hot water treatments to enhance shelf life of apricot fruit.

  • Characterization of sunburn damage to apple fruits and leaves

    The specific conditions of the formation of three different types of sunburn (sunburn browning, sunburn necrosis, and photooxidative sunburn) have been recently characterized on apple fruit. However, no information is still available on leaf damage. Therefore, the aims of this study were i) to extend the knowledge on fruit damage, ii) characterize leaf damage and iii) find relationship between fruit and leaf damage. The observations were made on 586 apple accessions in a gene bank orchard located in Hungary. The incidence of the three different types of fruit symptoms were recorded and based on the visual symptoms, two different types of leaf sunburn (sunburn yellowing and sunburn necrosis) were characterized. The most frequent type of fruit sunburn observed was sunburn browning. Photooxidative sunburn was found for less number of accessions, and only some accessions were affected by sunburn necrosis. Fruit were far more susceptible than leaves; (>60%) of the examined accessions were affected by fruit damage and (<3%) by leaf damage. Although a large number of accessions were affected, the percentage of fruit damaged within accessions was not that excessive; ~6% of the fruit assessed showed the symptoms of sunburn browning. Significantly fewer fruit were damaged by sunburn necrosis (~1%) or photooxidative sunburn (~1.4%) than sunburn browning. The percentage of leaves damaged within accessions were simlarly very low (~1%). Close relationship between fruit and leaf damage was found. Accesions with relatively heavily sunburned leaves usually had severe fruit damage as well. Leaves showing sunburn symptoms were usually closely located around those fruit which were sunburned severely. Leaf damage of sunburn was found on spur leaves in a great majority of the accessions damaged, shoot leaves did not seem to be susceptible to sunburn.

  • Some important growing characteristics of apple and apricot cultivars in two fruit growing regions in Romania

    The aim of this study was to determine some important growing characteristics of 5 resistant apple (Rajka, Rubinola, Topaz, Otawa and Goldstar) and 7 apricot (NJA 19, Goldrich, Harcot, Venus, Comando, Olimp, Cea mai buna de Ungaria) cultivars in two fruit growing regions in Romania from 2000 to 2003. Height of the apple trees (4-year-old tree) ranged between 1.96 m (cv. Topaz) and 2.39 m (cv. Rubinola). Diameter of the crown ranged from 0.96 m (cv. Goldstar) to 1.12 cm (cv. Rajka). The trunk diameter ranged from 3.5 cm (cv. Goldstar) to 5.0 cm (cv. Rajka). The surface of the trunk section was the lowest for cv. Goldstar (9.7 cm') and the largest for cv. Rajka (19.6 cm). Height of the apricot trees (4 years old tree) ranged between 2.69 m (cv. Venus) and 3.38 m (cv. NJA - 19). Diameter of the crown ranged from 2.59 m (cv. Comandor) to 2.77 m (cv. Cea mai buna de Ungaria). The trunk diameter ranged from 9.54 cm (cv. Goldrich) to 13.30 cm (cv. NJA - 19). Length of annual branches was the lowest for cv. Goldrich (45.1 cm) and the highest for cv. NJA - 19 (83.8 cm). Bud swelling of apricot trees began on 8 March for cv. NJA - 19 and ended on 11 March for the control cultivar (Cea mai buna de Ungaria). The blooming started on 16 March for cv. NJA - 19 and 27 Mach for the control cultivar. Duration of fruit growth was 89 days for cv. NJA - 19 and 128 days for cv. Comandor. When the temperature decreased to 1.5 °C (in 2001), percentage of viable pollen grains ranged between 48.86 % (cv. Olimp) and 91.57 % (cv. Venus). The germinating grains ranged from 31 % (cv. Olimp) to 90 % (cv. Harcot). Free pollination was the lowest for cv. NJA - 19 (29 %) and the highest for cv. Harcot (41%), while self-pollination ranged between 6 (cv. Olimp) and 11 % (cvs. Comondor and Harcot). Apple yield ranged from 16.65 t/ha (cv. Otawa) to 24.35 t/ha (cv. Rajka) and the differences varied from 4.45 t/ha to - 3.25 t/ha compared to the control varieties. Apricot yield ranged from 11.47 kg/ tree or 9.53 t/ha (cv. Cea mai buna de Ungaria), to 38.83 kg/tree or 27.34 t/ha (cv. Olimp) and the differences varied from 3 t/ha to 17 t/ha compared to the control varieties. Apple fruit weight ranged from 162 g (cv. Otawa) to 222 g (cv. Goldstar) and apricot fruit weight from 42.52 g (cv. Goldrich) to 68.38 g (cv. Comandor). Color, taste and aroma were very specific to cultivars.

  • Effects of excessive weather on the micro-climate of apple plantations under the hail protection nets

    The general utilisation of hail nets is spreading all over the world and in Hungary too. Hail nets are at present the only tool, which
    is able to prevent heavy hail damage in agricultural crops. Besides its numerous advantages, there are also deleterious side effects, e.g. it
    changes the microclimate of the crop stand. Our study aimed to observe the differences, which characterise the physical status of crop stands
    under the protection of hail nets compared with the stands in open air under various meteorological conditions: high or low temperature, calm
    or windy atmosphere and their possible combinations. The results indicated that on hot and calm days the difference may attain 3–4° C. On
    cool summer days, the difference was only 1.5 ºC. The hail net influences the relative humidity of the air, which means on ho summer days
    often 7-8% differences. Conditions of radiation may also differ conspicuously. Measurements prove the reduced global radiation on sunny
    summer days to 70%. This impairs as a rule the development of fruits reducing their quality and also its quantitative traits. Sunburn is, on the
    other hand, significantly prevented by shadow.

  • The effect of summer pruning on solar radiation conditions in apple orchards

    The aim of this study was to measure the solar radiation conditions in the tree and around the tree of pruned (summer pruning) and unpruned trees in an intensive apple orchard. When observing the daily trend of global radiation measured, there was a great reduction at midday hours due to the shadowing effect of the canopy. The reduction reached 70-75% before pruning, while after pruning it was only 20-25%. The relative radiation supply of the canopy space on cloudy days in the morning and late afternoon hours was higher than that of the sunny days. Mean differences between the day and night temperature reduced greatly in the period of intensive fruit colouration in August and September both in the plant stock and outside the plant stock.

  • Eco-friendly methods to control infection of Botrytis cinerea during propagation of grapevines

    Grey mould (Botrytis cinerea Pers.) is a pathogenic fungus which causes damage to the production of grapevine nursery plant materials especially on the stored canes or on graftings during the callusing period. The conditions, increased temperature and humidity, are ideal for the pathogen during the pre-forcing stage and in the storage Botrytis cinerea can easily infect the outbursting buds as large amounts of grapevine canes are stored in a relatively small place. The fungicide-based management is general in the prevention of gray rot infection but the palette of authorised chemical compounds is narrowed year by year due to the regulations of the European Union. Our aim is to develop an eco-friendly method which combines the use of natural materials with techniques used in organic farming. Effect of the ultraviolet-c light on the Botrytis cinerea was studied. The development characteristics of the pathogen were examined under daylight and dark conditions and experiments were set up with cow’s milk and acetic acid. UV-C light destroyed the developed conidia, however, the radiation stimulates the development of immature propagules. Larger quantities of conidiophores and conidia were formed in daylight compared to culture in the dark, while different conditions did not signifi cantly change the mycelial growth characteristics. The developed bacteria prevented the spread of pathogen mycelia during the test with cow’s milk in Petri dishes, although the smooth development of propagules that occurred did not change the vitality of the fungal colony. Furthermore the growth of Botrytis cinerea fungus mycelia was strongly inhibited by acetic acid.

  • Effect of variety and cultivation technology on phenols and antioxidant activity of sweet and sour cherry

    The goal of the present work was to compare different sweet and sour cherry cultivars and cultivation methods (bio/integrated) with respect to polyphenol content and antioxidant activity. The concentration of total polyphenols ranged between 880–1050 mg kg-1 of fresh fruit, whereas antioxidant activity expressed as TEAC was found to be between 5.4 and 10.3 mmol kg-1 for the sweet cherry cultivars examined. In case of sour cherry the level of polyphenols ranged between 1283 and 3490 mg/kg fresh edible part of the fruit. Antioxidant activity was recorded between 15–32 mmol kg-1 for the different sour cherry cultivars included in this work. After one-month storage at low temperature, the total phenols and antioxidant activity decreased by 2–40% in the sour cherry cultivars studied. The anthocyanin content in cherry cultivars was less (131–312 mg kg-1) than the135–1893 mg kg-1 found in sour cherries. Anthocyanin level was higher in samples produced under organic farming conditions than in those produced with integrated cultivation.

  • Apricot fruit chilling injuries during the cold storage affected by harvest maturity

    The study aimed to find the effect of different maturity classes (up to the days after full blossom) on the post-harvest life of 3 different apricot varieties Gold Cot, Bergarouge and Flavor Cot in Hungary. The fruit harvested in a particular time 65, 75 and 85 days after full blossom and divided to three maturity classes (Class 1, 2 and 3, respectively). Fruit stored in cold storage for 7 days at temperature 1 °C. Fruits were investigated in regard to physical parameters (weight loss, fruit firmness and soluble solid content SSC) and chilling injuries. The results showed that the varieties followed different ways in regard to response to different maturity classes. The maturity class I for all the tested varieties recorded the lowest weight loss, while with the increasing maturity stage the weight loss percentage for all the varieties recoded high values. The firmness decreased with delayed harvesting for all the varieties. Data of the chilling injuries showed that all the fruits which harvested at maturity I, recorded the highest percentage of fruit with CI (chilling injuries) at 0 (48.5, 37.25 and 38.75%) and CI class I (44.75, 35.75 and 39.75%) for Bergarouge, Gold Cot, and Flavor Cot.

  • Fruit production and research in Hungary - An overview

    Hungary is traditionally a food producer country. 63% of its total land area can be cultivated. Horticulture is one of the fundamental agricultural branches. The country has a moderate continental climate, with a mean temperature of 10 °C. The average hours of sunshine ranges 1,700 to 2,100 hours. Under the geographical condition in the Carpathian Basin the chemical composition of the fruits has a good harmony. The total fruit acreage is 97,000 ha with a crop of 800,000 to 900,000 tons yearly. In 1982 1,934,000 tons of fruit crop were produced since then it has decreased. The most important fruits are apple, European plum, sour cherry and raspberry. The percentage of apple reaches almost up to 60%. In the new plantings sour cherry, apple and black elderberry is popular. The most important fruit-producing region is situated at the North-eastern part of the country. More than 40% of Hungary's fruit production is concentrated there. In ranking the 2nd place is taken by fruit growing area in the middle of Hungary, where the production of stone fruits and small fruits has a considerable proportion.

    In the 70s and 80s of last century there was a developed research structure and wide range of research activity in Hungary. From that time the research capacity has considerably decreased first of all in the field of technological development. The main research area is fruit breeding and variety evaluation.

    Fruit scientists and fruit grower specialists are held together by the Hungarian. Society for Horticultural Sciences which has a membership in ISHS. Fruit researches and scientists having academic degree are belonged to the Horticultural Board of Hungarian Academy of Sciences.

  • Response of sour cherry cultivar `Érdi jubileum' fruits to modified atmosphere packaging after ethephon spraying

    Sour cherries are not adapted to long storage. The largest percentage of sour cherries reaches the consumer either directly or indirectly through the tin can or the frozen pack. Different concentrations of ethephon usually are used commercially to facilitate harvesting sour cherry fruits that this hormone influences on fruit quality. The objective of this study was effect of ethephon preharvest application on sour cherry fruits quality during storage period at modified atmosphere packaging was investigated. Modified atmosphere packaging used with 10, 15 and 75 percent for 02, CO, and N2 respectively that those held at 0 °C. This gas compounds synchronous to 0 °C temperature caused to increase shelf life postharvest of fruits. Fruit samples were evaluated at harvest date and after 6 weeks in storage. Skin fruit colour, pH, total soluble solids, titrateable acidity, sugar/acid ratio, firmness were monitored. Ethephon concentration influenced on total soluble solid, titrateable acidity, sugar/acid ratio and L* value. In more case, 225 ppm ethephon's concentration has no observable different to control samples. Thus, the best ethephon's concentration was 225 ppm ethephon's concentration.

  • Pollen viability of ‘Besztercei plum' clones depending on the effect of the year

    The percentage of dark staining pollen grains was higher in spring of 1996 than in the previous year. Data in 1998 resemble those of 1995, concerning the large amount of medium staining pollen grains in the majority of clones. Some clones produced excellent quality pollen also in the third year. whereas there were significant differences in other clones in various years.

    The warmer February-March period in 1995 induced an early blooming, and frost affected the orchard not only in winter months, but also immediately before and during blooming. Thus, frost was the possible cause of weaker quality pollen this year. In 1996 warming began a bit late, but it was not broken by drastic falls in temperature, except for the middle of April, when a smaller frost affected the orchard. It is likely that this frost did not influence pollen quality of `Besztercei' and 'Early Besztercei' plum clones significantly. In 1998 warming was continuous and steady, the orchard was not affected by frost immediately before blooming. In March, however, there was frost almost every day, according to daily minimum temperatures.

  • Simultaneous impact of the different water supply and year type on processing tomato yield

    A two year (2008 and 2009) open field experiment was conducted to study the effect of irrigation on the yield parameters and fruit components of processing tomato. Two different treatments were applied: regularly irrigated (RI), irrigation cut-off 30 days before harvest (CO), compared with unirrigated control (RF). The optimal water supply was calculated from average daily temperature. The aims of the study were to investigate the effect of different water supply on yield quantity. The regularly irrigated plant stands gave significantly higher yield, and unirrigated plants showed yield loss.Water supply had strong positive (R2=0.81) effect on marketable yield and average fruit weight (R2=0.78). Linear regression showed, that 46.5 mm more water supply caused 10 t/ha more marketable yield, and 13.4 mm more water supply caused 1 g more in the average fruit weight. The irrigation increased the Brix yield as well.

  • Phenological and fl ower morphological studies on different plum and prune cultivars

    20–22 and 5–7 years of kind collection of 100 cultivars studied phenology and fl oral morphological basis of characteristics in 1994–1998 and 2009–2011. The varieties were collected 3–5 trees in the gene garden, all were on C. 359 myrobalan seedling. Detecting differences between the test cultivars are suitable for stamps, and also show that relatively short-term observations are useful for describing the comparison, to distinguish varieties. The taxonomic ordination of cultivars and groups of fruits suitable to distinguish colors shown that the fl owering and ripening time, the nature of the reproductive organs and even Sharka-rate sensitivity is associated with those groups. Differences between the age of trees mainly sexual organs and fertility were shown. Affecting rate of climatic effects was not as large as the first line of erratic rainfall, air temperature spikes or possible values would be expected. The results of the breed, a gene bank of perception, in particular, the localization of hybrids, clone-type variants are useful in the analysis. Among other things – this is due to late-maturing, relatively well-stored temporarily, and not-blue plums, Sharka-infected tolerant or resistant varieties selection (cf. SURÁNYI 2013a-c) and will be disseminate in Hungary.

  • The effects of ACS (1-aminocyclopropane-l-carboxylate synthase) gene down regulation on ethylene production and fruit softening in transgenic apple

    A detailed examination of the production of ethylene and other ripening parameters during storage period has been undertaken in transgenic apple fruits, where the ethylene biosynthesis was inhibited by antisense ACS (l-aminocyclopropane-l-carboxylate synthase) gene. Data indicate down regulation of ethylene production, softening and spoilage in some transgenic lines. In some cases ethylene production was inhibited for over 90 percent, considerable reduction of softening and spoilage was observed probably due to the reduced activity of cell wall degradable enzymes. ACS activity was also monitored during ripening. The fruits of the best transgenic lines could be stored for minimum 4-5 months longer under 5 °C cold room storage conditions and one month longer at normal room temperature. This molecular approach can provide an alternative way to replace the commonly used and costly atmospheric storage of fruits.

  • Blooming time of some apricot varieties of different origin in Hungary

    There are a number of self-incompatible and partially self-compatible apricot varieties which need cross pollination for suitable yield. We have to know their blooming time to select the appropriate pollen donor cultivars. The blooming period of 20 apricot varieties was observed in four subsequent years. Blooming time was affected by temperature conditions very much. Varieties studied were assigned to three groups according to their blooming time. The rate of overlapping of important variety combinations was observed. Sufficient overlapping of blooming period for safe pollination is usually ensured within the same group of varieties or between varieties of the neighbouring blooming time groups.


  • Yield and composition of supercritical fluid extracts of different Lamiaceae herbs: Satureja hortensis L., Ocimum basilicum L. and Melissa officinalis L.

    In our work the effectiveness of supercritical carbon dioxide extraction was studied on volatile and nonvolatile components of Lamiaceae herbs. The aim of our investigations was to determine the optimal extraction parameters yielding high amount of volatiles and nonvolatiles in a desirable composition. As plant materials, dried and powdered cude drugs of Satureja hortensis, Ocimum basilicum and Melissa officinalis were chosen. Pressure (8-50 MPa), temperature (35-60 °C), time (5-60 min) and the ratio of modifier (5-50% methanol) of extraction were regulated. Results obtained by supercritical fluid extraction (SFE) were compared to that of the conventional extraction procedure, hydrodistillation (HD). In the case of Satureja hortensis, Ocimum basilicum and Melissa officinalis extract yield of SFE was comparable to the essential oil amount obtained by hydrodistillation from the same drug. Essential oil rich extracts were analysed by GC-FID, while extracts rich in nonvolatile compounds were analysed by HPLC. We have found that yield and quality of SFE extracts highly depend on the conditions of extraction.