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Impact of potassium fertilizers with different active ingredients and dosages on potato yield in experiments in the Nyírség region
71-74.Views:159A five-year experiment was carried out in the Nyírség on its characteristic soil type, brown forest soil to investigate the impact of three types of potassium fertilizers (potassium chloride, potassium sulfate and Patentkali) in three different dosages (80, 160, 240 kg/ha K20), under non-irrigated conditions applying the same dosages of N, P in all treatments (the applied amount was different between years) in 10 treatments including the control on 50 m2-parcels with 3 or 4 replicates. The test plant was cultivar Desiree, Kondor, Agria and Pannonia in 1998 and 2000, 2001, 2002 and 2003, respectively. The obtained yields were analyzed by an analysis of variance. Based on the five-year results, the highest yields on the acidic sandy and sandy loam soils were obtained in the treatments with 530-800 kg/ha Patentkali containing 30% of K and 10% of magnesium active ingredients in addition to the same amounts of N and P applications. The dosage of 530 kg/ha Patentkali contains 160 kg/ha K+ 53 kg/ha Mg active ingredients, while the dosage of 800 kg/ha Patentkali contains 240 kg/ha K+ 80 kg/ha Mg active ingredients. It can be concluded that the potassium chloride fertilizer (KCI) was the least effective as regards yields and Patentkali proved to be the most effective fertilizer, it had higher yields than even those of potassium sulfate, the difference in the 160 kg/ha treatments was 17.7%, which was a significant difference. Based on the results of the multi-year experiment, we recommend to apply 530 kg/ha Patentkali fertilizer (160 kg/ha K+ 53 kg/ha Mg active ingredients) for the fertilization in potato under non-irrigated conditions on the acidic sandy and sandy loam forest soils of the Nyírség, which proved to be the most effective treatment and with which we could obtain 30 t/ha yield in the average of the years (with the simultaneous application of N and P).
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Morphological examination of Hungarian apricot rootstock varieties
59-63.Views:178Apricot seedlings are one of the most widely used rootstocks in apricot growing of Hungary. The National List of Hungary contains 7 apricot seedling rootstocks varieties. The production of apricot graft on seedling rootstock takes 3 years from the seed production to the complete graft and requires more space. It is important to provide the trueness to variety name during this process. In the genus Prunoideae not only the plant but also the stone should have typical characteristics. It seems to be useful to examine the morphology of stone. The observed varieties were 'Tengeribarack C.1300', 'Tengeribarack C.1301', 'Tengeribarack C.1650', 'Tengeribarack' C.1652', 'Tengeribarack C.145', 'Tengeribarack C.1426' and 'Tengeribarack C.2546`.1t seems to be the most suitable characteristic to make a distinction between these varieties by stone, the ratio height of stone to lateral width of stone, the ratio of height of stone to ventral width of stone, the ratio of lateral width of stone to ventral width of stone and the ratio of ventral zone to ventral width. From not measured characteristic seems to be most utilizable to make distinction: the fusion of dorsal groove margins, the shape of apex, the presence of a mucro of the apex, the shape of stalk end in lateral view and the texture of lateral surfaces.
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In vitro plant regeneration from immature embryo axis and cotyledons of common bean (Phaseolus vulgaris L.)
93-97.Views:127Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus. It is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility. Recent advances in tissue culture have offered the opportunity to produce cultivars, which could not be obtained by conventional breeding methods. The use of tissue culture and genetic engineering is viewed as a logical approach to improve bean production. Gene transfer techniques will have a great impact on legumes. Although the concept of cell totipotency is widely proved, in vitro morphogenesis has not yet been achieved for a large number of cultivated beans. Regeneration protocols are strongly influenced by the genotype. In tissue and cell culture of beans, the factors controlling shoot morphogenesis and somatic embryogenesis are still unknown. The reported data suggest a possible way for future research.
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New trends in selection of poppy (Papaver somniferum L.)
69-75.Views:270Since the isolation of morphine by Sertümer in 1805 more than 40 alkaloids have been isolated from the poppy (Papaver somniferum L.). Some of them have high biological-pharmacological activity and economical importance, while others have none, or restricted ones. The increasing demand for poppy alkaloids is the consequence of the widening of the medical application of morphine and its related compounds: the quantity of morphine used for the treatment of pain reached a record level of 17.9 tonnes in 1997, compared with an annual average of 2.2 tonnes used during the period 1978-1983. However, the production of raw material (either opium, or dried capsule is produced) has to be re-evaluated taking into consideration the UN Convention signed in 1988 against Illicit Traffic in Narcotic Drugs and Psychotropic Substances. The countries were forced by the Convention to introduce new arrangements in poppy production including selection and introduction of new cultivars.
In the present work up to date results of poppy selection are reviewed explaining the biosynthetic and eco-physiological background of their alkaloid accumulation. The effectiveness and the possibilities of traditional selection methods as well as the probability of the application of biotransformation for producing cultivars accumulating low or high alkaloid content or plant material with special alkaloid spectrum (codeine, thebaine, narcotine) are discussed. The examples of Hungarian cultivars 'Monaco', 'Kék Gemona' and 'Tebona' are given in more detail.
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Fungicide resistant Trichoderma strains causing compost infection in shiitake production
67-70.Views:118Trichoderma infection represents the major problem of shiitake production in the growing house of the Research Institute at Kecskemet. Heavy infections occur mainly on the compost before spawn run. All the isolated strain belongs to the species Trichoderma harzianurn, but morphology of the colony indicates that there are more strains liable for the infection. Source of infection remained unclear but the Trichoderma strains can be considered as weed moulds rather than true pathogens of shiitake. All the Trichoderma isolates showed unusual high degree of resistance to fungicides.
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Role of nutrient supply in yield increase and quality improvement of spice pepper
83-86.Views:111Hungarian spice pepper powder is a unique product, a real hungaricum with its flavour and aroma compounds and seasoning effect. Its competitiveness with foreign spice peppers is ensured by its high biological value deriving from the specially Hungarian production and processing technology. Besides the traditional and highly manual labour intensive processing technology, there are some modern industrial technologies as well, where high quality can be guaranteed only by producing excellent base material (raw pepper pods). This is the reason which necessitates the rational development of the elements of the production technology, such as nutrient supply. Our objective was to offer a contribution to this aim by our trials in plant nutrition.
Experiments on the nutrient supply of spice pepper were set up in the 2003 growing season in order to decide whether yields and fruit composition parameters of pepper could be increased by means of increased K fertiliser doses with lower N:K ratios. Several forms of potassium were used, as well as applying microelement top dressings in the single treatments. It was found that the increase of N:K ratio from I:1 to 1:6 did not increase yields, but resulted in higher pigment and dry matter content. Microelement top dressing had a yield increasing effect at each N:K ratio. Higher potassium doses did not accelerate ripening.
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Disease progress of apple scab caused by Venturia inaequalis in environmentally friendly growing systems
56-62.Views:186Progression of apple scab epidemic in six apple cultivars, including two current and susceptible (Gala Must, Elstar), two old (Egri Piros, Darusóvári), and two resistant cultivars (Relinda, Releika), were described and analysed in a two-year-study, in two environmental-friendly growing systems (organic and integrated). Curves of disease progress, linear regression analysis of transformed disease incidence data and Area Under Disease Progress Curves (AUDPC) were used to characterise the epidemic processes of the selected cultivars. Cumulative disease progress curves showed continuous but asymmetrical scab development on the moderate or highly susceptible cultivars Gala Must, Elstar and Egri Piros, and on the tolerant or resistant cultivar Darusóvári and Relinda, in both systems. The cultivar Releika showed no symptoms either on fruit or leaf. In linear regression analysis, the best linearisation was given by logistic transformation. Adequate parameters leaf disease incidence rate, of obtained from a regression equation, were higher in the organic system than in the integrated system. Values of AUDPC showed great differences in leaf disease incidences among cultivars and between growing systems. AUDPC gave more differences for comparison of progresses of disease epidemic than growth rate of disease in different systems of disease control. Moreover, the obtained results were compared with similar studies on different pathosystems, and biological interpretations of the analyses are discussed below.
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Extreme weather events inWest Hungary
29-32.Views:229Agricultural production is a rather risky activity, as it is largely exposed to extreme weather events. This paper focuses on the frost and hail data in springtime, affecting fruit production in West Hungary. Regarding absolute minimum temperatures in April, significant increase of the standard deviations could be observed. Among the sites examined Nagykanizsa shows the highest risk values. According to our evaluation Nagykanizsa is not recommended to plant sour cherry, sweet cherry and apricot orchards because in some years spring frost will occur. If the plantation is in Zalaegerszeg, Szombathely, Gyôr or Keszthely than usage of frost prevention system is recommended. Siófok is the only area to grow apricot with good result.
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Analyses of temporal dynamics of brown rot development on fruit in organic apple production
97-100.Views:170In a two-year study, yield loss and temporal dynamics of brown rot development caused by Monilinia fructigena (Aderh. & Ruhl.) Honey were quantified and analysed in two organic apple orchards (Debrecen—Pallag and Debrecen—Józsa). The first infected fruits were observed at the beginning of August in both years and both locations, except for one occasion when the first infected fruit was found at the end of July. Temporal disease development was continuous up to harvest time in both years and locations. In the two years, pre-harvest yield loss on the trees amounted between 8.9% and 9.3% at Debrecen-Pallag and between 9.7% and 10.8% at Debrecen—Jozsa by fruit harvest. Incidence of infected fruits on the orchard floor ranged from 32.4% to 43.2% and from 53.3% to 61.9%, at Debrecen—Pallag and Debrecen—Józsa, respectively, by fruit harvest. Analyses of temporal disease progress showed that the best-fitted mathematical function was the power function in both orchards and years. Both parameters of the power function clearly demonstrated that incidence of brown rot on fruit increased faster on the orchard floor than on the tree. Moreover, the disease increase was faster at Debrecen—Józsa in most cases than at Debrecen—Pallag. Our results indicated that the strategy of disease management, the ripeness of the fruit and the presence of a wounding agent played an important role in the yield loss and in the temporal development of fruit disease incidence caused by M. fructigena in organic apple orchards. Biological and practical implications of the results are discussed.
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A review of the bee pollination research on temperate zone crop plants in the past decade: results and the need of further studies
7-23.Views:124Intense research was made on the pollination of cultivated crop plants in the temperate zone region during the past decade. Some 400 publications appeared on the subject and some additional 150 papers on the effect of pesticides to the most important pollinating insects, the honeybee and the wild bees. Progress of knowledge is discussed in this paper based on the most important publications. Most new results relate to field crops and deciduous fruit species and much less to field vegetables and small fruits. Great effort was taken to improve insect pollination of crops grown under cover. All these are connected to the utilization of the honeybee as a pollinating agent and much less to native or managed wild bees. However, a number of questions arose partly from the results of latest pollination research and partly from practical experiences in commercial plant production. These indicate several research tasks to understand and to solve the problems possibly in the near future. The questions concentrate on the effectiveness of bee visits in the pollination of individual crop plants and their different cultivars and on the reliable estimate of the overall amount of bees as well as on the control of bee density during the flowering periods of crops for optimal, controlled pollination in the changing environment of agricultural crop production.
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Comparative analysis of Hungarian Matricaria recutita (L.) Rausch. populations
81-85.Views:174Matricaria recutita L. is a traditional medicinal plant in Hungary and its drug is known as „Hungaricum", world-wide. Plant samples and seeds were collected from 12 different habitats of three significant geographical regions of Hungary in 2001. Morphological, production biological and chemical properties of samples were examined. In relation to the morphological characteristics, a negative correlation (r= -0.75) could be observed between the average height of the plants (height of flowering shoots) and the pH value of the soil. According to the composition of the essential oil, the populations accumulating typically chamasulene (10-20%), a-bisabolol (30-50%) or bisabolol-oxid (30-50%) could be completely distinguished. Concerning the flavonoid composition the quantity of apigenin-7-glucoside was outstanding in the populations originating from the Great Hungarian Plain, it has reached the concentration of 1.8-2.8 mg/g, while the samples collected in Transdanubia could be characterised by much lower level of apigenin-7-glucoside (around 1.5 mg/g).
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1-MCP and STS as ethylene inhibitors for prolonging the vase life of carnation and rose cut flowers
101-107.Views:210The effect of STS and 1-MCP on the postharvest quality of carnation and rose cut flowers was studied. Cut flowers of Dianthus c..aryophyllus L. cv. Asso and Rosa hybritia cv. Baroness were treated with silver thiosulfate (STS) at 0.4 mM with sucrose at 50 g 1-t and 1-methylcyclopropene ( I -MCP) at 0.5 g m-3 for 611.
Pretreatment with STS and 1-MCP significantly extended the vase life and minimized the % loss of initial weight of carnation and rose cut flowers comparing to the untreated control. The two chemicals applied inhibited the chlorophyll degradation and carbohydrate loss and hence, significantly improved the postharvest quality of carnation and rose cut flowers comparing to the control. Ethylene production by cut flowers was inhibited as a result of using these chemicals. In general, there were no differences between STS and (-MCP but the later does not have the heavy metal implications of STS treatment, and hence, using 1-MCP pretreatment for extending the vase life of carnation and rose cut flowers was recommended.
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Evaluation of cauliflower genotypes by different growing method
11-14.Views:229Cauliflower is currently cultivated on 350-400 thousand hectares in the world and around 1500 ha in Hungary. Its cultivation requires considerable expertise as it is sensitive to environmental factors and adequate nutrient supply. The aim of our experiment was to compare three genotypes (Abeni, SV5777 AC and Telergy F1) by different growing method – under plastic tunnel and on open field. On open field the planting was on 19th of March, 2018, the row and plant distances were 45 and 35 cm. Under plastic tunnel the transplants were set out on the same day with the same genotypes as on open field, with 55x45 cm arrangement. The results have proved that cauliflower curds from plastic tunnel was two times bigger than on open field. The more protected environment had a positive effect not only on the mass of the curd, but also on the biomass production. Among the examined genotypes Abeni showed the highest yield (about 1 kg/plant) under plastic tunnel. The shape of the head is important for the market. According to our data, we measured the width and height of the curd. Open field conditions induced wider curd with longer stem and caused lower product quality and weaker correlation between the parameters than plastic tunnel.
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Molecular characterization of apricot (Prunus armeniaca L.) cultivars using cross species SSR amplification with peach primers
53-57.Views:201Apricot takes an important place in Hungarian fruit production. Considering morphological characteristics of apricots it was concluded that the genetics background of European cultivars is very limited. Molecular markers and their use for genotyping have revolutionized the identification of cultivars. In a classic apricot breeding program, it is important to be able to establish unique DNA profiles of selections to identify them unambiguously and to determine their genetic relationship. Presently SSR is far the most frequently performed technique for genetic diversity studies. In this study there were used peach and apricot primer pairs from four different sources in order to examine microsatellite polymorphism among cultivars and investigate relationships among them. The possibility of cross species amplification among different Prunus species using SSR primers allowed us to use primers developed in peach to study genetic diversity in apricot. In this work, 90% of the primers used were able to amplify SSRs in apricot and more than half of them were polymorphic. With the 10 primer pairs utilized were proven to be sufficient to set unique fingerprint for several cultivars studied. The obtained dendrogram classified of the 45 cultivars included in this study into two major groups and several subgroups.
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Virus and virus-like diseases of grapevine in Hungary
25-36.Views:257Viruses and viroids are submicroscopic infectious particles which can cause disease symptoms on grapevine. These parasites are depending completely on the energy metabolism of the plant cell. To enter the host cell plant viruses depend on injuries or on transmission via invertebrates (insects, nematodes, etc.). Viruses are classified by many characters including particle morphology, host range and information content of the genome. At present about 70 viruses including 7 viroids infecting grapevine are known. In single or mixed infections they are potentially detrimental to the quality and quantity of grape production in any growing area of the world. Some viruses can cause severe economic damage in vineyards. In Hungary many important viruses and viroids have been detected in grape. This review summarises characteristics of viruses and the results of detection and characterization of virus and virus like diseases of grapevine in Hungary. The identification of the causal agent, its transmission, geographical distribution and the development of the diagnostic methods are also discussed.
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Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting
Views:114During in vitro multiplication and rooting of Vriesea splendens ’Fire’, 0.1, 0.2, 0.4 and 0.8 mg l-1 benzyladenine (BAP), benzyladenine-riboside (BAPR), kinetin (KIN), meta-topoline (MT), indole-butyric acid (IBA) and naphthalene-acetic acid (NAA) were added to basal Murashige and Skoog (1962) MS medium. As compared to the hormone-free control, plants developed significantly more shoots on medium supplemented with almost all cytokinins (excepting KIN), especially BAP resulted the highest multiplication up to almost 26 shoots. Enhancement of cytokinin concentrations increased shoot number (and in case of BAP, peroxidase activity) but decreased plant height and rooting parameters. Regarding root production, both auxins were definitely beneficial (0.2 mg l-1 NAA resulted more than 7.5 roots and higher auxin concentrations efficiently stimulate root elongation); however, KIN had similar effects. After a three-month duration time of acclimatization, we observed that plants which were previously cultured on medium containing certain cytokinins (KIN in all doses and 0.1 mg l-1 MT) or both auxins had greater survival, moreover, as negative after-effect, higher cytokinin concentrations reduced the number of survived specimens.
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Organic versus integrated apple growing: I. differences in soil and leaf parameters
57-60.Views:185The aim our study was to establish whether significant differences in nutrients uptake and quality of soil and leaf exist between organic and integrated grown apples. The study was performed at the orchard Fruit Research Station, University of Debrecen, at Debrecen-Pallag during 2002–2004. Macro and micro elements were measured in soil and plant samples. Analyses of variance of soil nitrogen data indicated highly significant differences between the two management systems (P < 0.001) for each examined nitrogen fraction. Analyses of variance of soil phosphate data indicated significant differences (P < 0.05) between the two management systems for orto-PO4 3– contents. Our data indicated that highly significant differences between the two management systems (P < 0.001) for magnesium, copper, and zinc; while significant differences between the two management systems was at P = 0.007 for calcium. Three year’s data of leaf phosphorus, sulphur and zinc were not shown significant differences between production systems. Nevertheless manganese and copper contents of leaves were higher in the organic orchard compared to the integrated one.
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Influence of abiotic factors on the green mass and seed productivity of the raw material of Levisticum оfficinalе c. Koch in the conditions of Transcarpathia
46-51.Views:244For the wide implementation of Levisticum officinalе C. Koch in Transcarpathia into production it is necessary to widen its sort composition, as it is rather poor in Ukraine. Solving this problem is possible by enriching the source material with the samples of different ecological and/or geographical origin, by studying the influence of abiotic factors on the formation of green mass and seed productivity, separating the specimens best adapted to the conditions of growing, involving them into the selection process and creating new, highly productive sorts on this basis. The scientists of the Transcarpathian State Agricultural Experimental Station (TSAES) conducted researches (during 2011-2016) on the adaptive potential of the source material of the type of Levisticum officinalе C. Koch, which counts seven samples (Ukraine – 5 samples, Poland – 1, and Romania – 1), the selection work on creating new varieties for the given region is being done. The investigations were made on soddy podzolic gleyed low-humus soils with low content of nutrients. The results were obtained on the basis of generally accepted methods of investigation, i.e.: laboratory, field, visual, measuring-weighing, biochemical, mathematically statistic, and comparative-calculating. In the conditions of Transcarpathia the spicy greens of the high quality L. officinalе C. Koch are being gathered on days 25-30, the output of the essential oil makes up from 0.72 tо 1.89% on c.d.s., depending on the climate conditions of growing. The plants pass through all the stages of development, the bush is 55.4 to 92.7 сm in height, the plants form a big amount of rosette-shaped stems (11-34 pieces), the productivity of spicy greens is between 9.9 and 42.1 t hа1. The scientists of TSAES created the following sorts – Mriya (А. с. 06131 Ukraine, first registered in the State register of plant varieties of Ukraine) and Coral (Pat. on the variety № 140830). Economic effect from growing of the Coral variety in the conditions of Transcarpathia is 10230 UAH/ha and the level of profitability is 28.4%. The geographic position and agroclimatic conditions of Transcarpathia, as well as the unpretentiousness of L. officinalе C. Koch. tо the conditions of growing contribute to the enrichment of the local flora with useful plants; and the rich chemical composition contributes to the spread of this culture in the given region.
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Effect of dry and wet years on primary inoculum source, incubation period and conidial production of Venturia inaequalis
53-58.Views:171The present study focuses on the characteristics of epidemics caused by the selected model-pathogen, Venturia inaequalis in relation to weather elements, with special respect to the increasing or decreasing effects of the specific weather elements. First, those weather elements are discussed which have a role in the development of scab epidemics. Subsequently, by accepting the thesis that climate change includes also weather extremes, an extremely hot and dry year (2003) and a colder year of higher than average precipitation (2004) were chosen as models. The presented examples verified that the variability of weather elements had had an undoubtable effect on the development of epidemics. The variability of weather elements manifests in the unusual behaviour of the pathogen, resulting in no or extreme disease epidemics. The extremities are well demonstrated by the fact, that in a year of drought an efficient protection can be achieved by considerably less applications than average, while in the next rainy year, the susceptible cultivar cannot be protected effectively even with such a high number of applications as is usual under humid Western-European climate conditions. It can also be noted, that the pathogen has a very good adaptability under unfavourable weather conditions. Consequently, more efficient management strategies should be developed for protection against the effects of extremities. However, it should be emphasized that it is very difficult to adapt to the variability and extremities of weather in the practice, because no long-term, accurate and reliable information is available about the variability of these elements.
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Water relations of apple and influence on fruit quality (minireview)
59-63.Views:302The saving of soil water content and the improvement of adaptability of plants to periodical insufficient water and use of deficit irrigation technology become more important because of the occurrence of frequently dry periods. The water use efficiency of apples can be increased by the choice of appropriate rootstocks and determination of water requirement of varieties that depend on their growing periods and climatic factors. Depletion of soil water resources need to develop efficient irrigation techniques for quality apple production. A new deficit irrigation strategy (PRD) has been developed that based on partial water supply of root-zone. This does not result a decrease in the sizes and yield of the fruit. The larger fruit size and lower firmness in frequently irrigated trees can result in excessive internal growth stresses that cause higher rate of fruit splitting. Many studies revealed the relationship between irrigation and yield quantity. Recent researches investigate the effects of irrigation on apple fruit quality particularly on the colouration and post-harvest quality. Use of cooling irrigation improves the development of coluour an apple fruit but its schedule can influence on the incomes. Aroma volatiles are responsible for odour and contribute to overall flavour of the fruit and its processed products. Deficit irrigation had only affects some volatile aroma but no the all of concentrations in apple fruit. In the future the high apple quality for consumers could be provided with improvement of transpiration-yield model based on the water requirement of varieties and economic irrigation schedules.
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Certification programme for production of virus-free propagating material of grapevine and its results in Hungary
39-43.Views:139In Hungary, detection of virus and virus-like diseases of grapevine began in 1960's at the Research Institute for Viticulture and Enology by János Lehoczky and his colleagues. At present, sixteen virus and virus-like diseases of Vitis vinifera are known to occur in Hungary.
Regular virological screening of grapevine varieties started in 1972. The present system of screening (visual selection, indexing, ELISA) has been established using methods with continuous improvement according to recommendations of international organizations.
In the first year symptomless grapevine plants are selected and marked during surveys carried out twice in the vegetation period: at about flowering and in the second half of September. At the first selection time plants are sampled for ELISA.
In the spring of the second year, overwintered canes are checked by woody indexing on 8 indicator species in the field.
In the third and fourth years the nursery is evaluated twice again. At the end, the marked grapevine plants, giving negative results on all indicators in every case, are considered virus-free.
In autumn of the fourth year, the virus-free material is planted out under screenhouse and also in a special mother block (nuclear stock) for maintenance and propagation.
Mother blocks of virus-free scion varieties have been established on 2 ha and those of rootstock varieties on 0.5 ha planted with the following number of varieties included in the national list: 71 European scion — and 12 rootstock varieties or variety candidates/clones. It is necessary to increase the area of Pre-base, Base and Certified stocks exclusively with tested virus-free (clean) material.
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Carbohydrate utilization of Erwinia amylovora in vitro
31-34.Views:153Nectar is a multi-component aqueous solution that promotes bacterial multiplication. The concentration of nectar in plant flowers is not stable since it is under the influence of environmental conditions, especially free moisture and relative humidity. Experiments were conducted with "artificial nectar" and directed along two lines: (1) determination of the optimal concentrations of carbohydrates for the growth of E. amylovora development (2) consumption of different carbohydrates besides basic sugars.
Solutions of "artificial nectar" were prepared in different compositions by changing the dominance of basic sugars (fructose — glucose —sucrose) in proportions of 2:1:1, 1:2:1, 1:1:2 and between concentrations of 10-0.6% (diluted with Basal minimum broth) in order to determine optimal conditions for the development of E. amylovora.
At a basic sugar concentration of 10% bacterial multiplication started and continued until I log degree (from 106 to 107 cfu/ml). At concentrations of 5% and 2,5 % cells developed with nearly the same kinetics (from 106 to 8x107 cfu/ml and from 106 to 9x107 cfu/ml, respectively). Multiplication was more pronounced and nearly the same at concentrations of 1.2 % and 0.6 % (from106 to 2x108 cfu/ml). At a basic sugar concentration 30% total sugars bacterial multiplication did not occur, while at 20 % it was negligible, not measurable photometrically.
At minimal concentrations of F, G, S (between 1-0.1 %) bacterial cells were still able to multiply, producing organic acids from sugars.
Our study showed that E. amylovora requires only a small amount of sugars (0.1%) for multiplication (acid production) while high concentrations inhibit multiplication. There was a negative correlation between sugar content and cell density. The optimal range of sugar concentration was at about 1%.
Effect of "less frequent carbohydrates" to E. amylovora multiplication was also determined using the API 50 CH strip. We could provide information on utilization of 39 carbohydrates by the bacterium at different categories as follows: Not utilized-, Slowly and weakly utilized-, Slowly and completely utilized-, Quickly and completely utilized carbohydrates. We suppose that carbohydrates that belong to the latter two groups could play an important role as nectar components in promoting E. amylovora multiplication in the blossoms of pome fruit trees.
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Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars
35-37.Views:385The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.
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Concentration of ‘Oblachinska’ sour cherry juice using osmotic distillation
31-34.Views:230Sour cherry is one of the most famous and characteristic Hungarian fruit what is also commonly used in food production as a colour additive. The sour cherry cultivar, ‘Oblachinska’ was shown to be a rich source of antioxidant compounds, including mainly anthocyanins. Valuable compounds in fruit juice – vitamins, polyphyenols etc. –are heat-sensitive molecules, which should be taken into account during the process of concentration to prevent degradation. Osmotic distillation seems a suitable option to product high quality sour cherry juice because this process does not require high temperature or pressure. Raw juice with approximately 15°Brix was used for the experiment and tried to concentrate up to 60° Brix where the water activity low enough to inhibit the microbiological deterioration. Before and after the process, TPC (total phenolic compounds) and FRAP (ferric reducing antioxidant power) was measured using spectrophotometric methods to determine the effect of the osmotic distillation. Our results point out that osmotic distillation is a promising method to concentrate sour cherry juice and prevent the loss of valuable compounds.
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Eco-friendly methods to control infection of Botrytis cinerea during propagation of grapevines
53-56.Views:268Grey mould (Botrytis cinerea Pers.) is a pathogenic fungus which causes damage to the production of grapevine nursery plant materials especially on the stored canes or on graftings during the callusing period. The conditions, increased temperature and humidity, are ideal for the pathogen during the pre-forcing stage and in the storage Botrytis cinerea can easily infect the outbursting buds as large amounts of grapevine canes are stored in a relatively small place. The fungicide-based management is general in the prevention of gray rot infection but the palette of authorised chemical compounds is narrowed year by year due to the regulations of the European Union. Our aim is to develop an eco-friendly method which combines the use of natural materials with techniques used in organic farming. Effect of the ultraviolet-c light on the Botrytis cinerea was studied. The development characteristics of the pathogen were examined under daylight and dark conditions and experiments were set up with cow’s milk and acetic acid. UV-C light destroyed the developed conidia, however, the radiation stimulates the development of immature propagules. Larger quantities of conidiophores and conidia were formed in daylight compared to culture in the dark, while different conditions did not signifi cantly change the mycelial growth characteristics. The developed bacteria prevented the spread of pathogen mycelia during the test with cow’s milk in Petri dishes, although the smooth development of propagules that occurred did not change the vitality of the fungal colony. Furthermore the growth of Botrytis cinerea fungus mycelia was strongly inhibited by acetic acid.