Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting

July 27, 2022

Copyright (c) 2022 International Journal of Horticultural Science

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

How To Cite
Selected Style: APA
Ördögh, M. (2022). Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting. International Journal of Horticultural Science, 28.

During in vitro multiplication and rooting of Vriesea splendens ’Fire’, 0.1, 0.2, 0.4 and 0.8 mg l-1 benzyladenine (BAP), benzyladenine-riboside (BAPR), kinetin (KIN), meta-topoline (MT), indole-butyric acid (IBA) and naphthalene-acetic acid (NAA) were added to basal Murashige and Skoog (1962) MS medium. As compared to the hormone-free control, plants developed significantly more shoots on medium supplemented with almost all cytokinins (excepting KIN), especially BAP resulted the highest multiplication up to almost 26 shoots. Enhancement of cytokinin concentrations increased shoot number (and in case of BAP, peroxidase activity) but decreased plant height and rooting parameters. Regarding root production, both auxins were definitely beneficial (0.2 mg l-1 NAA resulted more than 7.5 roots and higher auxin concentrations efficiently stimulate root elongation); however, KIN had similar effects. After a three-month duration time of acclimatization, we observed that plants which were previously cultured on medium containing certain cytokinins (KIN in all doses and 0.1 mg l-1 MT) or both auxins had greater survival, moreover, as negative after-effect, higher cytokinin concentrations reduced the number of survived specimens.

  1. Alves, G.M., Dal Vesco, L.L., Guerra, M.P. (2006): Micropropagation of the Brazilian endemic bromeliad Vriesea reitzii trough nodule clusters culture. Scientia Horticulturae, 110 (2): 204-207.
  2. Arnon, D.I. (1949): Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1): 1-15. doi: 10.1104/pp.24.1.1
  3. Arrabal, R., Amancio, F., Carneiro, L.A., Neves, L.J., Mansur, E. (2002): Micropropagation of endangered endemic Brazilian bromeliad Cryptanthus sinuosus (L.B. Smith) for in vitro preservation. Biodiversity and Conservation, 11: 1081-1089.
  4. Carvalho, C.P., Hayashi, A. H., Braga, M. R. (2013): Biochemical and anatomical responses related to the in vitro survival of the tropical bromeliad Nidularium minutum to low temperatures. Plant Physiology and Biochemistry, 71: 144–154. doi: 10.1016/j.plaphy.2013.07.005
  5. Da Silva A.L.L., Franco, E.T.H., Dornelles, E.B, Bortoli C.L.R, Quoirin, M. (2009): In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae). Iheringia Série Botânica, 64(2):151-156.
  6. Da Silva, A.L.L., Costa, J.L., Alcantara G.B., Carvalho, D.C., Schuck, M.R., Biasi, L.A., Scheidt, G.N., Soccol, C.R. (2012): Micropropagation of Nidularium innocentii Lem. and Nidularium procerum Lindm (Bromeliaceae). Pak. J. Bot., 44(3): 1095–1101.
  7. Dal Vesco, L.L., Guerra, M.P. (2010): In vitro morphogenesis and adventitious shoot mass regeneration of Vriesea reitzii from nodular cultures. Scientia Horticulturae, 125: 748-755. doi:10.1016/j.scienta.2010.05.030
  8. Droste, A., Da Silva, A.M., Matos, A. V., De Almeida, J. W. (2005): In vitro Culture of Vriesea gigantea and Vriesea philippocoburgii: Two vulnerable bromeliads native to Southern Brazil. Brazilian Archives of Biology and Technology, 48(5): 717-722. doi: 10.1590/S1516-89132005000600006
  9. Duarte, A.A., da Silva, C.J., Marques, A.R., Modolo, L.V., Filho, J.P.L. (2019): Does oxidative stress determine the thermal limits of the regeneration niche of Vriesea friburgensis and Alcantarea imperialis (Bromeliaceae) seedlings? Journal of Thermal Biology 80: 150-157.
  10. Faria, D.V., Simão, M.J., Cipriano, R., Werner, E.T., Soares, T.C.B., Aoyama, E.M., Lima-Gontijo, A.B.P. (2018): In vitro morphogenesis and micropropagation of Aechmea ramose var. ramose Mart. ex Schult. f. (Bromeliaceae) from leaf explants. In Vitro Cellular & Developmental Biology Plant, 54(5): 530-536.
  11. Freitas C., Carvalho, V., Nievola, C.C. (2015): Effect of sucrose concentrations on in vitro growth and subsequent acclimatization of the native bromeliad Vriesea inflata (Wawra) Wawra. Revista Biotemas, 28(3): 37-42.
  12. Guerra, M.P., Dal Vesco, L.L. (2010): Strategies for the micropropagation of bromeliads. In: Jain, S.M., Ochatt, S.J. (Eds.), Protocols for In Vitro Propagation of Ornamental Plants. Humana Press-Springer, New York, 47-66.
  13. Hamad, M.A., Taha, R.M. (2008): Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Scientia Horticulturae, 117(4): 329–334.
  14. Hamad, A., Taha R.M., Mohajer, S. (2013): In vitro induction and proliferation of adventitious roots in pineapple (Ananas comosus L.) cultivars of smooth cayenne and morris. Australian Journal of Crop Science, 7(7): 1038–1045.
  15. Hararap, F., Diningrat, D.S., Poerwanto, R., Nasution, N.E.A., Hasibuan, R.F.M. (2019): In vitro callus induction on sipahutar pineapple (Ananas comosus L.) from North Sumatra Indonesia. Pakistan Journal of Biological Sciences, 22(11). 518-526. doi: 10.3923/pjbs.2019.518.526
  16. Hernández-Meneses, E., Rangel-Estrada, S.E., López-Peralta, Ma.C.G., Guerrero-Hilario, A., Ortiz-Gil, G., Martínez-Bolanos, L. (2018): In vitro germination, viability and regeneration of Vriesea heliconioides (Kunth) Hook. ex Walp. plants. Rev. Fitotec. Mex., 41(2): 99-106.
  17. Huang, P.L.; Liao, L.J., Tsai, C.C., Liu, Z.H. (2010): Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth. Plant Cell, Tissue and Organ Culture, 105: 73-78.
  18. Jámborné Benczúr, E., Sinkó, Z., Ferenczy, A., Waldner, E. (2003): A Nidularium ‘Kertész Jubileum’ mikroszaporítása. Lippay János – Ormos Imre – Vas János Tudományos Ülésszak, Dísznövénytermesztési Szekció, BKÁE Természettudományi Centrum, Budapest, pp. 220–221.
  19. Knudson, L. (1946): A new nutrient solution for the germination of orchid seeds. American Orchid Society Bulletin, 14: 214-217.
  20. Koh, Y.C., Davies, F.T. (2001): Mutagenesis and in vitro culture of Tillandsia fasciculata Swartz var. fasciculata (Bromeliaceae). Scientia Horticulturae, 87(3): 225-240.
  21. Luther, H.E. (2014): An alphabetical list of bromeliad binomials. 14th ed. Sarasota: Marie Selby Botanical Gardens & Bromeliad Society Internatinal, 45.
  22. Makara Gy. (1982): Orchideák és broméliák – Trópusi őserdők növénycsodái otthonunkban. Mezőgazdasági Kiadó, Budapest
  23. Martins, J.P.R., Rodrigues, L.C.A., Conde, L.T., Gontijo, A.B.P.L., Falqueto, A.R. (2020): Anatomical and physiological changes of in vitro propagated Vriesea imperialis (Bromeliaceae) in the function of sucrose and ventilated containers. Plant Biosystems – An International Journal Dealing with all Aspects of Plant Biology 154(1): 87-99.
  24. Mathews, V.H., Rao, P.S. (1982): In vitro plant regeneration in lateral bud explants Cryptanthus bromelioides var. tricolor M.B. Forter. Plant Cell Rep., 1(3): 108–110. doi: 10.1007/BF00272365
  25. Mercier, H., Kerbauy, G.B. (1995): The importance of tissue culture technique for conservation of endangered Brazilian bromeliads from Atlantic rain forest canopy. Selbyana, 16(2):147-149.
  26. Murashige, T., Skoog, F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
  27. Ördögh, M. (2015): The effect of different cytokinins on chlorophyll content and morphological features of in vitro Nidularium ‘Kertész Jubileum’. International Journal of Horticultural Science, 21(1-2): 47-51. doi: 10.31421/IJHS/21/1-2./1157
  28. Paiva, P.D.O., Coelho-Naves, C., Ferreira-Dutra, L., Paiva, R., Pasqual, M. (2009): In vitro propagation of Nidularium fulgens Lem. Interciencia, 34(8): 593–596.
  29. Pickens, A. K., Wolf, J., Affolter, J.M., Wetzstein, H.Y. (2006): Adventitious bud development and regeneration in Tillandsia eizii. In Vitro Cellular & Developmental Biology Plant, 42: 348-353.
  30. Pierik, R.L.M., Sprenkels, P.A. (1991): Micropropagation of Tillandsia cyanea. Journal of the Bromeliad Society, 41: 9–12.
  31. Resende, C. F., Ribeiro, C., Mendes, G.C., Soares, C.Q.G., Braga, V.F., Cruz, B.P., Forzza, R.C., Peixoto, P.H.P. (2016): In vitro culture of Vriesea cacuminis L.B. Sm. (Bromeliaceae): an endemic species of Ibitipoca State Park, MG, Brazil. Iheringia Série Botânica, 71(1): 55-61.
  32. Rosa, W.S., Martins, J.P.R., Rodrigues, E.S., Rodrigues, L.C.A., Gontijo, A.B.P.L, Falqueto, A.R. (2018): Photosynthetic apparatus performance in function of the cytokinins used during the in vitro propagation of Aechmea blanchetiana (Bromeliaceae). Plant Cell, Tissue and Organ Culture, 133(3): 339-350.
  33. Sasamori, M.H., Endres-Júnior D., Droste, A. (2020): Conservation of Vriesea flammea L.B.Sm., and endemic Brazilian bromeliad: effects of nutrients and carbon source on plant development. Brazilian Journal of Biology, 80(2): 437-448.
  34. Shannon, L.M.; Kay, E., Lew, J.Y. (1966): Peroxidase isozymes from horseradish roots. The Journal of Biological Chemistry, 241(9):2166-2172. PMID: 5946638
  35. Tamaki, V., Nievola, C.C., Paula, S.M., Kanashiro, S. (2011): Alternative nutritional solutions for the culture of ornamental bromeliads. O mundo da Saúde, 35(1):91-97.
  36. Tillyné Mándy A., Honfi P. (2008): Növényházi dísznövénytermesztés. Inkart Kft. Budapest