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• A preliminary study on some features of two new resistant apple cultivars in a multi-row planting system

  11-14.

  Á. Csihon

  I. Gonda

  P. Vámos

  D. Barna

  I. J. Holb

  Views:

  1178

  The aim of this work was to report preliminary results on some features of two new resistant apple cultivars (cvs. ‘Galiwa’ and ‘Story Inored’) in multi-row system in the early bearing years of the orchard. Trees were planted in spring of 2015 in double row design (3.5 m+1.4 m x 1.0 m) with planting density of 5357 tree/ha. According to our results cv. ‘Story Inored’ presented more vigorous growth, than cv. ‘Galiwa’. Trees of cv. ‘Story Inored’ reached 2.7 m in the third year, but cv. ‘Galiwa’ could reach only 1.95 meter. Due to late spring frost 100% yield loss was observed in the second year. In 2017 cv. ‘Galiwa’ produced 5.3 kg/tree (25 fruit/tree), while cv. ‘Story Inored’ presented 7.7 kg/tree (50 fruit/tree). Average yield was 28.4 t/ha for cv. ‘Galiwa’, as ‘Story Inored’ reached 41.3 t/ha in the third year. Cultivar ‘Galiwa’ reached the required fruit size (79.1 mm), but its coloration was weaker (43% fruit surface color). Cultivar ‘Story Inored’ can be described with smaller fruit size (69 mm) and higher percentage of surface color (93%).

• Vegetative and generative properties of two apple cultivars ‘Galiwa’ and ‘Story Inored’ in a multi-row system

  34-38.

  A. Csihon

  I. Gonda

  I. J. Holb

  Views:

  916

  In a five-year (2015-2019) study, some vegetative and generative peculiarities of two resistant apple cultivars (‘Galiwa’ and ‘Story Inored’) were assessed in a young orchard with a multi-row training system. Based on our research, cv. ‘Galiwa’ showed significantly weaker growth, than cv. ‘Story Inored’, which was manifested in lower trunk cross sectional area (TCSA) and lower tree height. Cultivar ‘Story Inored’ reached the optimal tree height (3.1 m) at the age of four, but cv. ‘Galiwa’ could not achieve it neither in five-year-old trees (2.7 m). Cultivar ‘Galiwa’ showed 28.4-32.6 t/ha calculated average yield, while cv. ‘Story Inored’ produced 41.3-102.7 t/ha. Larger fruit size was found in cv. ‘Galiwa’ (72.7-79.1 mm) and smaller in cv. ‘Story Inored’ (66.9-69.2 mm). The fruit surface color was under 50% for cv. ‘Galiwa’ (43-49%), meanwhile cv. ‘Story Inored’ reached higher coloration (87-93%) and an excellent color intensity (4.8-5.0). Shape of cv. ‘Galiwa’ fruits was rather flat, than globular (0.83-0.84 shape index), as cv. ‘Story Inored’ was more elongated (0.95-1.00 shape index).

• DNA-based determination of suitable pollinating cultivars for the pear cultivar 'Carola' (Pyrus communis)

  15-19.

  H. Nybom

  J. Sehic

  H. Mattisson

  Views:

  293

  Pollen-limited fruit set has long been suspected in some relatively low-yielding orchards with the Swedish pear cultivar 'Carola'. Fruit was therefore harvested on 23 'Carola' trees in a commercial pear orchard. The seeds were germinated and five seedlings from each tree were sampled to determine which of the surrounding cultivars had been the most successful pollinators. Leaves of 'Carola', the 7 putative pollinating cultivars and the 115 seedlings were analysed with 6 RAPD primers. By comparison of the band patterns, paternity could be ascertained for 74 seedlings. The by far most successful pollinator was 'Clara Frijs' which had sired approx. half of the seedlings, followed by 'Herzogin Elsa', `Skanskt Sockerpiiron', 'Alexandre Lucas', 'Coloree de Juillet' and 'Doyenne du Cornice'. The latter is the maternal parent of 'Carola', and these two cultivars must therefore share one S-allele and hence can only be semi-compatible. In addition, 6% of the seedlings were in all likelihood derived from selling_ since they showed no bands that did not occur also in 'Carola'. Maximum distance between 'Carola' trees and suitable pollinators should not exceed 15-20 tn. Longer distances may produce a serious dearth of compatible pol­len as evidenced by the large percentage of seedlings derived either from selling. (25%) or from long-distance (> 40 m) pollen transfer (25%) when 'Carola' trees were surrounded by non-preferred pollinators.

• The role of black locust (Robinia pseudoacacia L.) in establishment of short-rotation energy plantations in Hungary

  41-44.

  K. Rédei

  I. Veperdi

  Views:

  561

  Establishment of short-rotation energy plantations for fuel production has been of international interest for many years. Energy plantation experiments in Hungary have been conducted for a longer time. In the country black locust ( Robinia ps eudoacacia L.) is one of the most important stand-forming tree species, covering approximately 23% of the forested land (410 000 ha) and providing about 19% of the annual timber output of the country. This fast growing species seems to be suitable for energy plantations as well. So, in Helvecia (Central­ Hungary, sand-soil region) two energy plantation s were established u sing common black locust and its cultivars improved in Hungary. The spacing variations of the common black locust were: l.5x0.3 m, I .5x0.5 m and l.5x 1.0 m. At the age of 5 the closest spacing ( 1.5x0.3m) produced the greatest annual increment in oven-dry weight (6.5 t ha·1 yr- 1). In the trial with black locust cultivars planted in spacing of 1.5xl.0m, at the age of 7 the highest annual increment in oven-dry mass was produced by the cultivar ' Ulloi' (9.7 t ha-1 yr- 1) followed by the common black locust (8.4 t ha-1 yr- 1) and the cultivar 'J tiszkiseri (1.6 t ha·1 yr- 1). The trials have verified that in temperate climate the increment in oven­ dry dendromass of black locust energy plantation s has ranged from 6 to 12 t ha·1 yr·1. On the basis of the trials' evaluation the quantity of dendromass mostly depends on site quality, species and cultivars, as well as on the initial spacing (plants per hectare).

• Effect of modified atmosphere package on apricot fruit storability

  30-32.

  A. Ezzat

  Views:

  407

  The aim of this work was to study the effect of modified atmosphere package (MAP) on apricot storability. Apricots (Prunus armeniaca L.) cultivar Jumbo cot were harvested at commercial ripening stage. The fruits were treated with different storage treatments as following: i) control storage for 13 days at 1 °C, ii) stored fruit at 1 °C for 10 days then 3 days at shelf at 25 °C, iii) fruit stored in MAP at 1 °C for 13 days and iv) fruit stored in MAP at 1 °C for 10 days then 3 days at shelf at 25 °C. Data showed the positive effect of MAP in keeping the apricot fruit for long time with better quality than the control fruit. MAP showed positive effect by recording the lowest fruit weight loss, the highest firmness and lowest chilling injury and fruit decay.

• Some aspects of disease management of cherry leaf spot (Blumeriella jaapii) with special reference to pesticide use

  45-49.

  I. J. Holb

  P. Lakatos

  F. Abonyi

  Views:

  1318

  In this review, some aspects of disease management of cherry leaf spot (Blumeriella jaapii) are summarised with special reference to pesticide use. In the first part of the review, we show the non-chemical control approach (e. g. removal of fallen leaves, planting resistant cultivar) against leaf spot. In the second part of the review, the effect of pesticides including fertilizers (urea) and fungicides on cherry leaf spot are discussed. Special attention are given to the fungicides of copper, dodine, captafol, captan, benomil, chlorothalonil, sterol demethylation inhibitors (e.g. fenarimol, fenbuconazole, myclobutanil, tebuconazole), and strobilurins about their effectiveness against cherry leaf spot. In the final part of the review, possibilities of cherry leaf spot control are discussed in integrated and organic cherry orchards.

• Preliminary results of fruit quality of resistant sour cherry clones in 2014

  31-34.

  B. Vaszily

  Views:

  358

  The richness of Hungarian sour cherry cultivars in the world is unique; they can be consumed in many ways. Sour cherry is a Hungaricum and has excellent fruit quality and nutritional value. Nowadays the demand for chemical-free fruits is increasing. Reducing the number of chemical applications can be achieved most effectively by the use of resistant varieties, technological developments and cultivar innovation. The domestic and international sour cherry breeding programs use almost exclusively the resistant ‘Csengôdi’ clones as a basis. They are mainly Monilinia and Blumeriella resistant and have higher dry matter and antioxidant content. These pathogens influence not only the qualitative and quantitative parameters of the fruits, but may play an indirect role in the deterioration of the overall condition of the trees as well. The cultivation of resistant varieties can greatly reduce the pesticide load of the environment, so we can produce fruit with excellent nutritional value with fewer chemicals. Our aim is the expansion of the sour cherry assortment by introducing new  resistant cultivars. With this we can contribute to the spreading of environmentally sound production technologies and supply “super fruits” to the consumers.

• The inheritance and durability of scab resistance in apple progenies

  39-46.

  M. Tóth

  A. Pedryc

  Views:

  450

  In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.

  The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.

  The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.

• Molecular analysis of strawberry cultivars using RAPD, AP-PCR and STS markers

  24-28.

  A. Balogh

  E. Kiss

  A. Szőke

  F. Dénes

  L. Heszky

  Views:

  353

  Seventeen strawberry (Fragaria x ananassa Duch.) cultivars representing the national list of Hungary, were subjected to RAPD, AP—PCR and STS analysis. Of the 31 decamer and oligomer primers tested 26 primers produced polymorphic patterns. 45 polymorphic fragments were analysed, ranging between 200-2800 by in size. Based on the data, similarity coefficients (Jaccard index and Simple matching coefficient) were calculated, and dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA). The dendrograms only partly reflect the known pedigree data. Specific RAPD markers were identified for cultivars F5, Pocahontas and Rabunda.

• Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars

  35-37.

  B. Molnár

  Sz. Szabó

  Á. Veres

  I. J. Holb

  Views:

  1146

  The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.

• Influence of environment in electro-chemical quality of tomato

  17-20.

  S. Kaciu

  V. Shala-Mayrhofer

  N. Mirecki

  S. Aliu

  K. Jezik

  Views:

  366

  The aim of the research was to investigate the impact of different cultivation environments of tomato cultivars, the electro-chemical and the qualitative traits of the tomato fruits. The quality of tomato fruit harvesting was evaluated at the Laboratory of Institute of Horticulture and Viticulture University of Natural Resources and Applied Life Sciences, Vienna. Two hybrids of tomatoes were tested Belle-F1 and AmaF1, in two locations and two different treatments (open field and plastic tunnels). Model of experimental fields was based on random method and included three replications. Following parameters were analyzed and tested: pH, Rh, P-Value, Nitrates and the content of vitamin C. The values of pH for treatment in the open field and indoor production system were not significant (4.47 to 5.05). For P-Value derived from the two treatments the maximal values were without significant differences, while minimal values were significant. Study has shown also that the highest values of nitrate content (16.34 mg, open field) compared to average value differences were + 0.384 mg or 43.83% higher. In indoor production the nitrate content was lower in compared with plants cultivated in open fields, so these differences were +3.81 mg or 26.4 0%, and highly significant for both levels. In relation to vitamin C content, the highest value was identified in the indoor systemof 298.6mg, with variations from the overall average of 16.43% or + 42.15 mg. The coefficient of variation values for both levels was of 29.96 respectively 27.31%.

• Organic apple growing using sanitation treatments against apple scab

  11-13.

  F. Abonyi

  I. J. Holb

  Views:

  635

  Effects or sanitation practices were evaluated on primary and autumn infection by Venturia inaequalis in an organic apple orchard at Eperjeske on the moderately scab-susceptible apple cultivar Jonathan in 2005 and 2006. Evaluated sanitation practices were: i) collection of fallen leaves in autumn; ii) destroying fallen leaves by disc cultivation in autumn; iii) spraying fallen leaves with 2% lime sulphur in autumn; iv) spraying fallen leaves with 2% lime sulphur in autumn and then collection of fallen leaves in autumn; and v) untreated control. In both years, most sanitation practices reduced significantly (P< 0.05) scab incidence in the primary infection periods compared to untreated control. The highest leaf scab incidence was observed in the untreated plots (26:2 and 24.3% in 2005 and 2006, respectively), while the lowest was in the treatments of spraying fallen leaves with 2% lime sulphur combined with collection of fallen leaves (11.7 and 12.3% in 2005 and 2006, respectively).

• RAPD analysis of grapevine hybrids and cultivars

  63-66.

  J. Halász

  J. Korbuly

  T. Deák

  Gy. D. Bisztray

  Views:

  446

  Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations.
  
  

• Nectar production of pear (Pyrus communis L.) cultivars

  67-75.

  P. Benedek

  G. Kocsisné Molnár

  J. Nyéki

  Views:

  1248

  Detailed studies were made on the nectar production of 44, 16 and 18 pear cultivars, respectively, in a cultivar collection of pear during three consecutive years with highly different weather in the blooming. Results clearly show that pear does not necessarily produce small amount of nectar as stated in the world literature. In fact, pear can produce extremely high amount of nectar sometimes much higher than other temperate zone fruit trees species but its nectar production is highly subjected to weather, first of all to air temperature. Low nectar production seems to be more frequent than high one and cold weather can prevent its nectar production at all. On the other hand, results corroborate to the earlier statements on the low sugar concentration of pear nectar. There is a highly significant negative correlation between the amount of nectar produced by pear flowers and its sugar concentration (r = -0.52, n = 291, p< 0.001 for 1996, r = -0.34, n = 197, p< 0.001 for 1998). Sugar concentration in individual flowers may be up, to 40% in exceptional cases but generally it is well below 20%. Very high figures for sugar concentration in pear nectar at the literature seem to be incomprehensible. In contrast of some earlier statement in the literature no real difference could be established in the nectar production of pear cultivars, based on much more measurements than in earlier studies. Very low sugar concentration in pear nectar can explain the fact that the overwhelming majority of honeybees are pollen gatherers at pear trees even in the case of exceptionally high nectar production.

   

• Some biological features of cherry leaf spot (Blumeriella jaapii) with special reference to cultivar susceptibility

  91-93.

  I. J. Holb

  Views:

  1383

  In this review, some important features of biology are summarised for cherry leaf spot (Blumeriella jaapii). In the first part of the review, the geographical distribution of the pathogen and the causal organism are described. Disease symptoms and disease cycle of cherry leaf spot are also shown. Special attention is given to hosts and then several cherry cultivars.are described in relation to their susceptibility to cherry leaf spot.

• Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin

  91-95.

  B. Gosztola

  E. Németh

  S. Sárosi

  K. Szabó

  A. Kozák

  Views:

  732

  In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.

  In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.

  According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.

• Effect of life cycle on the production of mullein (Verbascum phlomoides L.)

  123-125.

  Zs. Bodor

  E. Németh

  Views:

  387

  Aim of the present investigations was the optimalization of the production of the annual cultivar `Napfény' of Verlxiscum phlomoides L. Quantitative data on morphology (growth, leaf and flower size, branching) yield and content of active materials (mucilages, flavonoids) were studied at six sowing times.

  We established, that sowing time may be one basic factor in the production of the annual variety. The major yield was obtained by sowing either late autumn (end of October) or early spring (middle March). At these plots the fresh mass of the flowers was 257-288 g/plant, the drug mass 28-29 g/plant, by 45-70% more than that of the mean of other treatments.

  It was established, that under optimal cultivation conditions the annual form of mullein may reach higher individual yields than the plants of the indigenous wild growing population.

• Yield and fruit quality response to foliar application of biostimulants in an apple orchard

  17-23.

  Adam Csihon

  Marianna Sipos

  Imre Holb

  Views:

  635

  Nutrient supply plays an important role in fruit production technology. Among the methods of nutrient management, the application of foliar fertilization has become an essential tool to harmonize the vegetative and generative performance of fruit trees. This study was conducted to determine the effect of four foliar fertilizers (Bistep, brown juice, Trichoderma harzianum, Clonostachys rosea) on yield and fruit quality in an apple orchard with the cultivar ‘Pinova’, at the University of Debrecen in Hungary. Trees were trained to a slender spindle canopy with a spacing of 4 × 1 m. Treatments were applied five times during different phenological phases in 2021 and 2022. Based on our results, biostimulants had a positive effect on fruit yield and fruit quality, but extreme weather events also influenced the data. In 2021, the effect of the treatments on yield data could not be observed (10.1–13.5 kg/tree; 0.16–0.24 kg/cm²). However, in 2022, plants sprayed with Bistep, T. harzianum, and C. rosea reached 28.7–31.2 kg/tree (0.33–0.46 kg/cm²), while control trees produced only 19.8 kg/tree (0.20 kg/cm²). Fruit size development improved with an increase of 2–6 mm over the two years. Among the treatments, Bistep was able to enhance fruit surface coloration to a greater extent in 2021, as red skin color reached 44% for this foliar fertilizer, while control trees presented only 27%.

• Effects of DMI and strobilurin fungicide combinations on scab and powdery mildew incidence in apple production: a preliminary study

  37-39.

  I. J. Holb

  M. Kocsis

  J. Kruppa

  F. Abonyi

  Views:

  927

  The aim of our study was to evaulate the effectiveness of four DMI and strobilurion fungicide combinations in a season-long disease management programme against apple scab and powdery mildew in two integrated apple orchards on cultivar Jonagold. The four spray programmes were: i) standard, ii) DMI dominant iii) strobilurin dominant, and iv) DMI and strobilurin dominant. Apple scab incidence as generally high in both orchards in the standard fungicide treatment plots ranged between 21.8 and 26.4%. Leaf scab incidence was higher in all treatments compared to fruit incidence. Scab incidence was generally higher at Mándok compared to Ófehértó. In both locations the lowest scab incidence was assessed in DMI dominant treatment ranged between 5 to 10.7%, which was not signifi cantly different from the DMI and strobilurin dominant treatment. Powdery mildew incidence was generally low in both orchards in all fungicide treatments ranged between 0.002 and 0.5%. Although there were no signifi cant differences among treatments, the lowest powdery mildew incidence was assessed in the DMI and strobilurin dominant treatment in Mándok and in the strobilurin dominant treatment in Ófehértó.

• Brown rot blossom blight and fruit rot of apricot in Hungary

  139-141.

  G. Drén

  Z. Szabó

  M. Soltész

  J. Nyéki

  I. J. Holb

  Views:

  3744

  The aim of our two-year study was to assess incidence of brown rot blossom blight and fruit rot caused by Monilinia laxa in 2003 and 2004. Assessments of incidence were made on cv. Bergeron (susceptible to brown rot) in a flatland and a hilly growing area (at Cegléd and Gönc, respectively). In both locations, plant protection was performed according to the integrated fruit production guidelines and small untreated plots were set up for each cultivar in both years. In 2003, when weather conditions were dry and hot, brown rot incidence was low (less than 10%) on both blossoms and fruits. Monilinia laxa did not cause significantly different blossom blight and fruit rot at the hilly (Gönc) area compared to the flatland, not even in untreated plots. However, in 2004, when spring and summer weather conditions were wet and cold, Incidence reached 95% for blossom blight and 33% for fruit rot in the untreated plots. Blossom blight incidence was 1.5-2 times higher in the flatland area compared to the hilly growing area. During the blooming period of apricot, two (at flower bud stage and at full bloom) and three (at flower bud stage, at full bloom and at petal fall) fungicide applications were necessary for the successful control at Gönc and Cegléd, respectively. The difference between the two orchards was due to the fact that blooming started one week later in the hilly region (at Gone) than in the flatland region (at Cegléd), therefore, the critical weather period coincided with blooming in the orchard in the hilly region only partially. Fruit rot incidence was similar in both regions as the amount and distribution of rainfall were similar during the fruit ripening period.

• Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis

  69-72.

  A. Pedryc

  J. Halász

  R. Hermán

  A. Hegedűs

  Views:

  507

  Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of some Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S<sub>1</sub>S<sub>2</sub>) and `Harcoe (S₁S₄) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.

• The uses of wolfberry (Lycium barbarum L.) as a fruit in an international breadth of view

  7-13.

  R. Sóspataki

  M. Józsa

  G. Simon

  Views:

  624

  Lycium barbarum is known exclusively as an ornamental plant in Hungary, and is planted so, as popular belief deem it a toxic plant. The plant’s fruit receives great respect in countries abroad, thus, due to its favourable content values the Lycium has achieved the title of ‘biological gold mine’. The last couple of years has seen the import of Lycium shrubs and its corresponding products, dried goods or in the form of various processed products which have been marketed and sold at extreme prices (under name of Goji, Wolfberry, Lifeberry). Our goal was to examine the similarities of the content values of wolfberry found in Hungary and those cultivated abroad. Along with Miklós Józsa the domestic Lycium population was surveyed based on foreign examples, between the years 2009 and 2011. Those defined sweet and largefruited were selected for further investigations to be set into a clone repository. This clone repository– which contains 67 different clones from a number of regions of Hungary – was established in the nursery of Dr. Miklós Józsa, located in the city of Szombathely. The phenological and morphological characteristics and the fruit ripening and quality indicators of plants in the clone repository were investigated. Six ‘best’ clones – selected based on flavour, disease-resistance and vegetative characteristics (plant size, fruit set, yield and fruit size) were analysed based on their content values. The control plant was a cultivar imported by a delivery service, found also in growing. In addition to the results of the selection, the results of the content values of the six selected ‘best’ clones (total soluble sugar content, glucose-fructose ratio, carotene content, FRAP value) is documented in this paper. Further, based on the resulting information the possibilities of the fruit’s utilization are suggested. Significant differences were measured in the vegetative characteristics of the clones (plant size, fruit set, yield and fruit size) and of those of
  its inner content values. The resulting properties are considerable in regards of the plant’s introduction into cultivation and also utilization. Based on growing characteristics, more favourable clones were selected than the foreign varieties already in growing. Those clones selected by us have had similar or better content values than foreign breeds. The investigation of these content values is still in progress.

• Specialities of the vegetation start and level of primary fruit set affect fruit quality

  17-22.

  N. Rakonczás

  P. Dremák

  I. Gonda

  Views:

  755

  Thinning is a h ighly crucial point of the apple production technology. According to results of numerous studies the earliest thinning is deemed to have the best amending effects. There can be considerable difference between trees of the same cul tivar and age in a plantation in respect to their flowering, in the numbers of fruits set and also in their canopy volume. Thus it can be crucial -just l ike in the case of pruning- to establish a fruit thinni ng su i ted for specif ic characteristics of actual trees. This experiment was established to examine how does primary fruit set (fruit load before thinning) and further on specific (fruit/TCS cm2) and absolute (fruit/tree) fruit-load of the trees affect quality attri butes at harvest. Our experiment was establ ished in a plantation on medium-tight loamy soil in north-east of Hungary. Harvest date was determined w ith the joint observation of the calendar date, starch-index, flesh firmness, background colour and fruit weight. Three fruit-load levels were established based on local experience and on data of several years, I Ot/ha lower and higher besides the advised optimum yield in the same orchard with slender-spindle shaped 'Gala must' cultivar standing on M9 rootstock in 1m x 3,8 m spacing. In the establishment of the 15, 25 and 35t/ha fruit-load levels on 20-20 apple trees total number of fruits set was counted at each tree. After this number of apples due to be removed was defined using an objective index on the basis of trunk cross sections (fruit/TCScm2) (Lafer, 1999). The following attributes were measured: weight (g), flesh firmness (lb/cm2) total soluble solid content (Brix %) and total titrate-able acid content (g/J).From the data on sugar and acid content quality index (Pomona value) was determined (Thialult, 1970). We could ascertain, that in an orchard, of the same aged but in concern to trunk cross sections somewhat different trees besides the specific index (apple/TCS cm2 the absolute fruit load (fruit/tree) can also be an important data, that has considerable effect on the internal quality. Secondly we could observe, that higher level of fruit load before thinning (primary fruit set) negatively affects quality index of the apples irrespective of the specific fruit load level (fruit/cm2 TCS) set later. Results underlines necessity of the earliest chemical thinn ing.

• Fire blight (Erwinia amylovora) susceptibility of old Hungarian apple cultivars

  35-38.

  M. Tóth

  K. Honty

  M. Hevesi

  Views:

  493

  The aim of the Hungarian apple breeding program started in the Department of Fruit Science was to find resistant apple cultivars against major diseases (scab, powdery mildew, fire blight). The outbreak of fire blight (Erwinia amylovora) in 1996 motivated us to search new resistant sources principally from old traditional apple cultivars. First of all, cultivars have been gathered since 1997 from Carpathia (Visk) and evaluated between 2001 and 2003. In this recent study evaluation of resistance of old Hungarian genotypes to fire blight collected from the English National Fruit Collection (Brogdale, Faversham) is presented.

  13 old Hungarian apple cultivars in 2002, and 38 genotypes in 2005 have been evaluated. We used `Idared' and 'Jonathan M41' as susceptible controls and 'Liberty' and 'Remo' as resistant ones. Shoots of two-year-old potted plants were inoculated with a mixture of virulent E. amylovora isolates (Ea2, Ea60, Ea67) at a concentration of 5 x 10⁸ cells/ml. Resistance of apple cultivars was evaluated weekly, four times after inoculation by disease severity of symptoms. Numbers of bacterial colonies in 1 cm length shoot were determined in the fourth week after infection.

  8 cultivars in 2002 and 9 cultivars in 2005 displayed notable resistance to fire blight based on one-year data. Based on the coincident data of both years, out of the cultivars collected also from Carpathia ‘Pónyik', and `Sikulai' were found to be resistant and gene sources additional old Hungarian valuable apple genotypes could be selected: `Szabadkai szercsika' and `Tordai piros !davit'. The cultivar `Szemes alma' originated from Visk has proved to be recurrently resistant.

  The number of bacterial cells in shoots of the investigated cultivars correlated with the severity of symptoms. With this method, which was introduced by us earlier, we can screen cultivars displaying weak visible symptoms, which cannot be proposed as a source of resistance because of their latent infection.

• The selection and cultivation of Tilia clones tolerating polluted urban environment

  82-86.

  E. Jámbor-Benczúr

  Z. Sinkó

  M. L. Reményi

  Z. Ifjú

  Views:

  554

  Two urban stress tolerant Tilia clones were selected by the Department of Floriculture and Dendrology. The mother trees of the Observed clones were found in an alley of linden trees, settled on a traffic island of a busy two-lane road.

  Tilia hybrid 'Saint Stephen' has a beautiful cone-shaped crown, the leaves are bright green and they keep their green colour for much longer time than the leaves on the other trees in the alley. In the nursery the Tilia hybrid 'Saint Stephen' was budded on T. cordata, T. platyphyllos and T. argentea and it had good compatibility with every rootstock. The average height of the one year old buddings was 200 cm and the buddings kept their good growing capacity in the following years as well. They had an outstanding growing capability comparing with the other Tilia cultivars.

  Tilia platyphyllos 'K3' clone has similar cone-shaped crown. The growing vigor and urban stress tolerance seems to be better than Tilia hybrid 'Saint Stephen'.