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• In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants

  15-20.

  E. Enkhbileg

  M. G. Fári

  E. Kurucz

  Views:

  790

  Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.

• Cumulative vector intensity and seed potato virus infection in Hungary

  61-64.

  Z. Basky

  Views:

  213

  Aphids were collected by Moericke yellow pan traps placed in the potato fields. The cumulative vector intensity is an index that describes the vector abundance and their propensity to transmit PVY (3). The vector intensity was obtained as the number of known PVY vector species caught and multiplied by their relative vector efficiency value. Cumulative vector intensity for the season was calculated by accumulating species-specific vector intensity values at each trapping date. In those places where the number of PVY vectors caught by yellow pan traps were the highest (1194, 1495 and 663, 570, respectively), the cumulative vector intensity was also the highest (322 and 570, respectively). This high vector intensity resulted in high progeny tuber infection 21 and 31 %, respectively. In those years when the cumulative vector intensity did not reach the value of 10 until the end of June and the beginning of July the proportion of PVY infected progeny tubers met the requirements of the standard, it was less than 5 %. The cumulative vector intensity seems to be a reliable way to forecast virus threat to seed potato. Both seasonal variation and vector abundance is reflected in cumulative vector intensity, above all propensity of different vector species is included in the calculation. As the virus translocation from leaves to tubers takes 12-14 days. Therefore it is imperative that immediately after weekly trap catches cumulative vector intensity values are calculated, as when values reach around ten growers in seed potato growing region will have 12 days to execute killing leaves and stems of seed potatoes.

• Dr. Ottó Orsós, the forgotten Hungarian pioneer in plant tissue culture

  9-13.

  M. G. Fári

  Views:

  430

  The knowledge of tissue culture deserves attention in respect of understanding the development of universal biology. This study intends to contribute to the past of the plant tissue culture by such data of the history of science which have been unprocessed so far. It seems that the life-work of the Hungarian biologist, Dr. Ottó Orsós is a missing and essential link between those early plant hormone researchers and the representatives of the pioneers of tissue culture schools who have contributed substantially to the development of the modern in vitro plant morphogenesis and plant cell biology. Orsós cultured kohlrabi tuber cubes on White culture medium in a sterile manner. This way, he could efficiently direct the in vitro morphogenesis of the kohlrabi, the regeneration of its shoot and root, and the formation and steps to subculture of pure callus tissues in 1938. He supported the correctness of its statements by means of detailed anatomical examinations. Orsós successfully rooted and aclimatized complete regenerated plants. We may as well call the above system — in remembrance of the creators of the original concept — "Haberlandt-Orsós model". Between the publishing of his main paper in 1938 and 2003, a period of 65 years has lapsed. On the occasion of this anniversary, we bow before this forgotten pioneer.

• Variability of the data indicating the fertility of different plum varieties

  51-55.

  Z. Szabó

  J. Nyéki

  Views:

  1170

  Self-fertility and fertility at conditions of open pollination in plum varieties is strictly determined by genetic factors. However, rates of fruit set are highly variable according to growing sites as well as to seasons, which may result from a couple of inner and outer conditions, but mainly from the method applied in seizing the facts of fertility in the experiments planned including the number of replications of treatments. During three successive years, 4-16 trees of each of the four plum varieties have been selected and 16-64 branches were tagged either for checking their fertility as pollinated freely or isolated excluding the access of foreign pollen. The data of fruit set have been processed in order to determine the variability of the data, subsequently, the number of replications necessary to make reliable decisions. Both autogamy and open pollination displayed multiple differences between branches and trees studied.

  A number of 20 branches are needed yearly for each variety, the branches should be distributed on 5 trees at least for checking the autogamy, whereas on 10 trees for the results of open pollination. Each variety and treatment should be represented in three seasons, at least because of the different weather conditions.

• Antioxidant capacity, C-vitamin and anthocyanils content in the fruits of elderberry (Sambucus nigra L.) types

  77-79.

  N. Szabó

  B. Z. Sipos

  É. Stefanovits-Bányai

  Views:

  507

  : Elderberry used to be a common medicinal plant since long. Its ripe fruit and inflorescence has been collected. Cultivation however, started in Hungary recently, with the early 90-es. Initially, has been planted, the 'Haschberg' cultivar. Our Department started first with the selection of favourable genotypes in the Hungarian spontaneous populations of the species, we aimed to find clones, which are earlier ripening than 'Haschberg', moreover, superior in any of the agronomical or quality traits. Present study deals with 19 new selected clones by their content of vitamin-C, anthocyanins and their antioxidant capacity. Environmental stresses are inducing, increased, accumulation of free radicals in living organisms. To evoid the harmful effects of the free radicals, we need to consume antioxidant compounds (flavonoids, anthocyanins, vitamins, etc.) In the literature there are quite a few references related to the anthocyanins and antioxidants of the elderberry. Measurements prove that substantial differences exist among the population of the species as for the content and quality of those compounds. In anthocyanin content, the difference between the highest and lowest values was 3000 mg/I, whereas in antioxidant content this value was 6001.1M. Those types, which contained less anthocyanin (<5000 mg/I), produced lower antioxidant capacity, whereas the highest anthocyanin content was associated with the strongest antioxidant activity. C-vitamin content varied between 36.5 and 96.4 mg/100g. These values provide daily human requirements by their consumption. Summing up all parameters, examined the best type was Cinkota 1.

• Goals and results in improvement of biological background of medicinal plant production

  20-27.

  É. Németh

  Views:

  376

  The choice of varieties among medicinal plant species is relatively small, compared to other horticultural crops. In Hungary, only poppy (Papaver somniferum) and mustard (Sinapis alba) have several cultivars. Recognising the problem, in the recent years breeding activity has been intensified all over the world, in spite of financial, technical and legal difficulties. The article reports on the results of breeding at the Department of Medicinal and Aromatic Plants of the Szent István University, 11 varieties of which has been officially registered till 2000. Main goals of genetic improvement are: increasing of the production capacity of utilised plant organs, enhancement of active material accumulation capacity, improvement of sensory quality and technological properties. The most often applied methods are selection, and recently, cross breeding, the results of which can be measured on new materials of caraway (Carum carvi), hyssop (Hyssopus officinalis), marjoram (Majorana hortensis), poppy (Papaver somniferum), etc. Efficacy of breeding work is established by additional, regular research on the genetics, physiology, floral and reproductive biology, chemosyndromes of medicinal plant species.

   

• Economic aspects of applying hail protection nets in apple plantations

  99-101.

  F. Apáti

  M. Soltész

  Views:

  842

  The up to date intense apple growing in Hungary is capable to produce yearly about 500-1000 thousand Ft/ha income, which means that the investment of 4 000–5 000 thousand Ft is returned by producing 10-20% net income. The economic balance may, however, be upset by the damage caused by hail, quantitative and qualitative, with an apparently increasing frequency experienced in each third or fourth year. Estimates prove that each hail causing 50% damage may reduces the income proportionately to the capital by 1.5–2.0 percent points, which means a serious threat for the economy of apple production. According to calculations, a plantation producing 30–40 t/ha yields would not be able to raise incomes compensating the investment of 7.0–10.0 million Ft/ha, let alone the frequencies of hail damages. Consequently, 50–60 t/ha yields are needed to become successful, and in planning of new plantations those high yields are aimed with hail nets. An additional difficulty is represented by a lack of financial resources to install hail protection nets.

• Pollen tube growth in sweet cherry (Prunus avium L.) styles following fully compatible, half compatible and incompatible pollinations

  63-68.

  Zs. Békefi

  Zs. Halász

  Views:

  268

  In vivo as well as in vitro pollen tube growth studies along the style were performed, each with two pairs of sweet cherry cultivar combinations by means of fluorescence microscopy. In vivo studies showed that the percentage of pollen tubes penetrating the middle and basal section of the style was higher in the fully compatible 'Margit' x 'Alex' combination than in the half compatible `Germersdorfi 3' x `Alex' cross. The year effect was significant at P=0.] probability level. All pollen tubes in vitro stopped at the upper third of the style in the incompatible 'Vera' x 'Van' cross, whereas in the half compatible 'Alex' x 'Van' 50% of the pollen tubes penetrated to the lower third of the style. By in vitro fluorescence microscopy, it was possible to distinguish half compatible combinations from incompatible ones. Results obtained by in vivo technique only were much ambiguous.

• Floral bud development, blooming time and fertility relations of some Romanian apricot varieties in Hungary

  41-43.

  L. Szalay

  J. Papp

  Z. Szabó

  J. Nyéki

  Views:

  986

  Due to the geographical situation of Hungary the introduction of late ripening apricot varieties holds great interest. In apricot production floral bud development during winter, blooming time, and the fertilisation properties are important factors. These characters were studied in six late ripening Romanian apricot varieties (Callatis, Comandor, Litoral, Selena, Sirena, Sulmona) in Szigetcsép representing the northern site of the lowland growing area. During the mild winter of 1997/98 the dynamics of floral bud development in the Romanian varieties was a few days slower during the whole examination period compared to Gönci magyar kajszi. Their meiotic divisions occurred between 1 and 5 February. Next winter the meiotic division started later at 28 February, due to the cold weather. In these conditions the dynamics of bud development was similar in all the varieties. Averaged over seven years blooming of the Romanian varieties started 1-3 days later than in Gönci magyar kajszi. All the Romanian varieties showed self-fertility to some extent, however, application of other pollen donor sources is necessary for the safety of production, with the exception of Callatis.

• Propagation from root cuttings for black locust (Robinia pseudoacacia L.) improvement in Hungary: a review

  39-41.

  Zs. Keserű

  K. Rédei

  J. Rásó

  T. Kiss

  Views:

  597

  Black locust (Robinia pseudoacacia L.) is a valuable stand-forming tree species introduced to Europe approximately 400 years ago from North America. Today it is widely planted throughout the world, first of all for wood production. In Hungary, where black locust has great importance in the forest management, it is mainly propagated by seeds. But since the seed-raised plants present a great genetic variation, this type of propagation can not be used for Robinia’s improved cultivars. In the Hungarian black locust clonal forestry, propagation from root cuttings can be used for reproduction of superior individuals or cultivars in large quantities. However, this method demands more care than raising seedlings from seeds and can be applied with success in well-equipped nurseries.

• Economic figures of apple production at national level of Hungary

  J. Felföldi

  Views:

  377

  After the change of the political system the main looser was in Hungary the fruit-growing branch. The yields varied at high amplitudes, and the production increased slowly in spite of multiple planting activities. The European Union ranked fruit production “loosely” to the products, which allows the extension of its markets. Thus I decided to analyse the economic relations of the period between 2002 and 2008 in order to reveal the main effects of movements. The pictures are the resultants of a heterogeneous population, which cannot be influenced on the level of enterprises. But they are utilised for the recognition of challenges and trends on the level of branches of economy. The immediate costs increased the production monotonously, which cannot be compensated by a thrifty management of the general costs. Thus costs of production increased dramatically, whereas the marketing of products could not realise the values.

• Cost and profit conditions in the Hungarian intensive apple production

  65-68.

  F. Apáti

  X. Wang

  J. Racskó

  Views:

  478

  In this study I investigated the cost and profit conditions and the efficiency of intensive, qualitative apple production on the basis of a data collection carried out in ventures of high standard production. I concluded that the intensive apple production has an extremely high cost requirement, the production costs are approximately 1500 to 1600 thousand HUF per hectare. In an average case, a production value of 2000 thousand HUF per hectare may be reached, which may fluctuate in a wide interval during the years. Considering the above mentioned, a net profit of 400 to 500 thousand HUF may be realized in one hectare. It should be highlighted that regarding the present marketing conditions, realizing the appropriate profit may be expected only by producing 30 to 40 tons per hectare yields and 80 to 90% food quality ratio.

• Eicosapentaenoic acid production by Phaeodactylum tricornutum under different culture condition

  133-136.

  J. Kutasi

  F. Szederkényi

  I. Ott

  M. László

  Views:

  235

  Phaeodactylum tricornutum UTEX 640 strain of microalgae was screened under different culture conditions for their capacity to produce eicosapentaenoic acid (EPA) the most abundant polyunsaturated fatty acid (PUFA). In our experiments, the effect of sodium chlorid, nitrogen source, phosphate, initial pH, as well as the CO₂ content of the medium on production of the eicosapentaenoic acid (EPA) by P. tricornutum were investigated. The EPA content of biomass was enhanced by the low pH of the medium, with increased concentrations of B₁₇ vitamin and nitrate, and also with decreasing concentrations of sodium chlorid. The EPA is most likely associated with polar (membran) lipids and the role of EPA appears to be involved with membran permeability in microalge. The synthesis of phospholipids, enhances the EPA content of the cells, as expected. The maximum EPA yields were observed under optimum culture condition 43 — 48 mg/g of dry cell weight.

• The genetic background of resistance to common bacterial blight in newly identified common bean lines on the basis of inheritance studies

  77-81.

  E. Mergenthaler

  Bisztray Gy.

  Views:

  240

  Common bacterial blight (CBB), caused by Xanthomonas campestris pv. phaseoli (Xcp). is a major disease problem of common bean (Phaseolus vulgaris L.). The inheritance of resistance in Xrl and Xr2 lines to two isolates of Xcp was studied in the F2 and F3 popu­lations from the crosses between these lines and the Masay variety (susceptible to Xcp). Segregation patterns indicated that different single recessive genes presumably in coupling phase linkage determined the resistance to the HUN and EK-1 1 strains of Xcp in both lines. The presence of some minor, modifying genes beside the monogenic genetic background of resistance was also observed. Xrl and Xr2 lines represent valuable new monogenic genetic sources in resistance breeding to CBB.

• Self-incompatibility in pears (Pyrus communis L., Pyrus serotina Rehd. and Pyrus ussuriensis) Review

  87-91.

  J. Halász

  A. Hegedűs

  Views:

  459

  Self-incompatibility system and allele pool of three different pear species, European pear (Pyrus communis), Japanese pear (P. serotina) and Chinese pear (P ussuriensis) are displayed. Several inconsistencies and the absence of the harmonization of three different allele series are revealed in the European pears. By collecting data from several reports eight incompatibility groups of Japanese pear cultivars could be established. A self-compatible genotype is analysed in details and shown to be a stylar-part mutant. As Japanese pear was the first fruit tree species from which S-ribonucleases were identified, the history of S-genotyping from the beginning to the latest achievements and technical developments can be also monitored from the experiments enumerated. In Chinese pears, seven S-alleles and one incompatibility group could be identified.

• The brown rot fungi of fruit crops (Monilinia spp.): I. Important features of their biology (Review paper)

  23-36.

  I. J. Holb

  Views:

  536

  The brown rot fungi of fruit crops (Monilinia spp.): Important features of their biology (Review paper)

• Anatomical structure of stem in the above- and underground portions of Rosa rugosa suckers

  89-91.

  R. Fouda

  Views:

  313

  Comparitive histological studies were made on the underground and the aboveground stem parts of Rosa rugosa taken from one year old suckers. The underground stem parts were characterized with thicker primary cortex, phloem and pith, weaker phloem fibers, wider cambial zone, medullary rays, xylem and phloem rays as compared with the aboveground stem parts. The most marked differences in the underground stem parts were in the wide cambial zone and in the development of some adventitious roots.

   

• Mechanical and physical control in apple orchards as preventative fungal disease management

  19-21.

  I. J. Holb

  Views:

  731

  In this minreview, mechanical and physical control against apple fungal diseases among non-chemical control approaches were summarized. This overview listed five groups of mechanical and physical control methods:  pruning, removal of inoculum sources, shredding of leaf litter, burying of inoculum sources and flaming of leaf litter. These methods were shown to reduce succesfully infection potential of inoculum sources in orchards and these non-chemical control measures are one of the most essential approaches for preventative fungal disease management. However, most of these methods are not widely spread in the apple-growing practice due to relatively low control efficacy, medium to high labour costs and/or time limits during the season.

• Preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard

  35-37.

  I. J. Holb

  Views:

  568

  In this study, the objective was to report a preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 11 and 20 on shoot and between 9 and 24 on fruit. Number of asymptomatic plant part ranged between 85 and 109 on shoot and between 133 and 206 on fruit. Disease incidence ranged between 13.8 and 17.6% on shoot and between 9.1 and 11.3% on fruit. Disease aggregation index ranged between 0.098 and 0.228 on shoot and between 0.043 and 0.108 on fruit. One of the four trees showed significant within canopy aggregation of disease for shoot powdery mildew symptoms in both years. For leaf powdery mildew, all tree exhibited random patterns in both years.

• Effects of integrated and ecological growing technologies on the growth and development of fruiting structures in new apple plantations

  31-33.

  P. Dremák

  Views:

  375

  On the Horticultural Station of the Debrecen University, Pallag, two year old apple plantations of two varieties (‘Pinova’, ‘Golden Reinders’) have been studied, in autumn 2011. The growth of the cross section area of the trunk and the central axis were measured and compared to evaluate the effect of two alternative growing technologies: integrated and/or ecological. ‘Pinova’ proved to grow more intensely than ‘Golden Reinders’ independently from the growing technology. The effect of the technology, however, was expressed in ‘Pinova’, where the integrated technology produced more vigorous growth than the ecological one. The effect of varieties on growing intensity was more distinct than the effect of growing technologies. Under the ecological growing system, the difference between the varieties studied is more accentuated regarding growing intensity. By that reason, in ecological growing plantations of varieties with moderate vigour ought to be stimulated by all possible techniques (as pruning, nutrition etc.).

• Investment analysis of Hungarian apple-orchard and fruit storage projects

  157-162.

  F. Apáti

  Z. Lakner

  Views:

  630

  There are profound, long-term changes in world apple production and trade. The former hegemony of Europe in apple production doest not exists any more, among the most important apple exporters the emerging economies have a growing importance. The globalising apple market means new challenges of traditional producers. This is especially true for Hungary, which has been the most important apple exporter in terms of quantity thirty years ago, but now its production hardly covers the domestic demand. A necessary precondition of the modernisation is the re-construction of plantations and the cold-storage system. Analysing the economic effi ciency of apple production and cold storage, it is obvious, that a necessary precondition for the modern, competitive apple production is the availability of cold-storage facilities. Neither the apple-production, nor the cold-storage can not evaluated separately from each other. Under current Hungarian conditions there is a need for active state support for the establishment of cold-storage facilities.

• Variation between some apricot varieties in regard to flowering phenology in Boldogkôváralja, Hungary

  7-9.

  A. Ezzat

  L. Amriskó

  G. G.

  T. Mikita

  J. Nyéki

  M. Soltész

  Z. Szabó

  Views:

  998

  The aim of this study was the estimation of blossoming of 14 apricot cultivars in Boldogkôváralja in 2009, 2010 and 2011 seasons. And this will help growers to select appropriate varieties to their weather conditions. For this target the blooming period of 19 apricot varieties of different origin was observed in three subsequent years. There was no large difference in the beginning of blooming in the different years, and the greatest variation between the start date of flowering was about 1 to 3 days as the place of experiment site near to northern border and also, length of flowering period of apricot trees is also inversely related to date when blooming started. The little differences in flowering dates and flowering periods due to the high temperature through the three seasons of study.

• A study of processes active in regeneration of different sweet cherry varieties

  55-57.

  B. Vaszily

  Views:

  392

  Cherries belong to the group of fruit trees, which require little attention in pruning. The process of getting bald branches is less accentuated, which is associated with the light requirement of organs changing along with the aging process. The growing habits of the varieties (length and number of growing shoots, etc.) is closely related with the productivity of fruiting parts. The ability of regeneration and the functional effectiveness of fruiting structures are expressed by the distribution according to their age. The differences registered justify the necessity to develop pruning policies for each particular type of variety. For that purpose, we have to examine the age dependency of the processes related to regeneration, which may serve as starting point of a pruning strategy. Growth of some parts should be stimulated in order to develop or regenerate fruiting parts. Some branches need to be rejuvenated, partially, as an antidote to progressive senescence. Every intervention is aimed to strengthen the development of the most productive constructions. Reactions induced by rejuvenation should be expressed in fruiting intensity. A pruning program adapted to the particular variety as well as to the actual phase of a rotating pruning system should anticipate results.

• International competitive analysis of wine producer small and midsize companies on enterprise resource management

  23-25.

  Zs. Sümegi

  A. Molnár

  Views:

  311

  In our research using primary data sources we are searching for satisfactory evidences, that the impressive economical growth of China has a strong impact on Chinese grape and wine production, wine market. However grape cultivation has long tradition in China, modem grape wine and wine production has only started 20 years ago. During my research I have collected the very rare and unreliable information on this theme to make a picture of Chinese grape and wine production. Using primary sources describe the present situation of Chinese grape industry, verify the supposition that the rise of Chinese grape crop land includes the growth of the total size of wine grape plantations. After this verification I make a comparative analysis of Chinese and Hungarian grape cultivation costs, to confirm, that grape and wine producing is a more requital activity in China than in Hungary.

• Impact of sodium-selenate on the growth of radish (Raphanus sativus L.) seedlings in vitro

  113-115.

  É. Domokos-Szabolcsy

  I. J. Holb

  J. Prokisch

  B. Kovács

  Zs. Veres

  M. G. Fári

  Views:

  652

  Selenium (Se) is an essential trace element for animals, microorganisms and some other Eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although it is well known that some species among higher plants are able to accumulate selenium in their tissues, but others are not able to do so, and there is evidence that selenium is needed for the growth of algae, meanwhile the question of essentiality of Se in vascular plants is unresolved. We aimed to study the in vitro growing and to characterise some physiological properties in radish (Raphanus sativus L.) seedlings treated with 0 to 200 mg/1 sodium-selenate. The results showed that lower (2 mg/1) concentration sodium-selenate increased the biomass as well as the total antioxidant capacity of seedlings. The seedling's selenium content showed linear correlation with the sodium-selenate content of the medium.