Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to devel...op a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
European perch (Perca fluviatilis) is a native predatory fish in Hungary, and a promising new species of fresh water aquaculture nowadays. The European perch can be characterized by a high stress sensitivity during the intensive rearing, thus the optimization of environmental conditions has significant importance in the early life...stages of fish. The aim of our study was to determine whether the light intensity and darkening of the water by humic acid affect the survival and growth performance of European perch juveniles. The experiment lasted for 28 days. Rectangular aquariums were arranged in 3 lines, the test environment consisted of 24 units. The experiment was set up with 10–10 fish per aquarium, total of 240 individuals. The average wet body weight of the fish at the start of the experiment was 1.69 grams. During the experiment, 6 treatments in 4 replicates were set up. In the first row, we set up 305.6±66.0 LUX (L), in the second row 118.0±24.4 LUX (C), and in the third row (D) 17.0±8.6 LUX illumination was set up. In each row the water of four aquariums were darkened by the addition of humic acid (H) (L, LH, C, CH, D, and DH). At the end of the experiment high survival rate was observed in all treatments. Examining the survival rate, the best results were found in the strongly illuminated treatments (L; LH- SR%=100±0.00). The observed mortality was caused by cannibalism. Regarding the individual body weight, the best results were shown by the treatments where the aquariums were illuminated with lower light intensity (C: 4.66±0.33 grams) and the aquarium water was darkened by humic acid (CH: 4.93±0.15 grams). The results of the C and CH groups were significantly better compared to the other treatments, however, they did not differ statistically from each other. The darkening of the water had a positive effect on the individual body weight of fish reared under full light (L: 3.73±0.28 grams; LH: 4.33±0.28 grams), whereas in the case of fish reared in the lowest illumination, the addition of humic acid did not affect the results (D: 3.78±0.15 grams; DH: 3.80±0.26 grams). In case of SGR (C: 3.64%/day and CH: 3.74%/day) and FCR (C: 0.84 g/g and CH: 0.78g/g) the best results were obtained by the C and CH treatments, also.
A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-l
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees.
The environmental factors to which surplus water can be assigned (topography, soil, groundwater, vegetation etc.) can be subject to special analysis and the randomness of the occurrences can be limited. The results of these procedures are surplus water risk maps of the areas, which can be utilised in land use planning. The risk map of the resea...rch site was created with overlaying digital category maps of the determining factors (hydraulic conductivity, convexity, critical probability of ground water level and land use).
The effects of crop rotation, nutrien supply and crop protection technologies, as well as the appearance of the main ear- and leafdiseases
(powdery mildew, helminthosporium leaf spot, leaf rust, fusarium) were studied on the crop yields of winter wheat variety MV
Pálma during the 2009/2010 crop year. The experiments were conducted in tri
five nutrition levels, with the use of three crop protection technologies (extensive, conventional and intensive) at the Látókép Research Site of
the University of Debrecen, Centre of Agricultural Sciences. Our results proved that the appearance of leaf- and ear-diseases were
significant in the wheat cultures during the 2009/2010 crop year, because of the rainy, warmer than usual weather, the lodging, and the huge
vegetative mass developed. The most severe infections by the four examined diseases after pea and corn pre-crops were observed at
extensive crop protection levels, when fertilizers were used at the highest dose.
Following corn pre-crop, in the case of all the three crop protection technologies the maximum rate of wheat yield results were achieved
at N150+PK level. The highest yield was reached at intensive crop protection level (6079 kg ha-1). In triculture, in case of all the three crop
protection technologies the maximum yields were achieved at N50+PK level; in extensive technology 5041 kg·ha-1 yield, in conventional
technology 6190 kg ha-1 yield was realised, while in the intensive technological model the yield was 7228 kg ha-1.
The relationship between yield and fertilizer amounts, the rate of pathogen contaminations, crop protection technologies and pre-crops
was defined with correlation analysis in case of different crop rotations during the 2009/2010 crop year. Based on the results of the
experiment, we found that in stands after corn pre-crop strong positive correlation was established between the crop protection level and the
crop yield (0.543), the nutrient levels and the emergence of the four examined pathogens, and between the nutrient levels and the yield
(0.639). Extremly strong positive correlation was observed between crop protection and yield (0.843) in triculture. Strong positive
correlation was detected between the nutrient levels and the presence of the four examined pathogens, as well as between nutrient and
lodging (0.688). Strong negative correlation was between the crop protection level and the four examined diseases both in biculture and
In the past decade, the population of the Hooded Crow has shown significant growth in Debrecen, Hungary. The aim of this study was to become acquainted with the nest-site selection behavior of Hooded Crows in urban spaces. While our research revealed that the Hooded Crow is not particular when it comes to selecting among tree species for nestin...g, we did notice differences regarding nesting height. Hooded Crows living in the city build their nests higher up than those living outside Debrecen’s city limits. We also discovered a slight, insignificant difference between nesting heights and tree species, which is probably due to the different characteristics of the given tree species. As for nesting heights, we found that in typical urban habitats there were no relevant differences. However, when we compared these habitats in pairs, it came to light that nesting heights -when comparing solitary trees - wood segments and tree rows - wooded segments- did show significant differences, which can be explained by the various conditions provided by the habitats mentioned.
In summary, the following results emerged from our research:
1. The Hooded Crow prefers approximately the same nesting heights in all kinds of habitats, urban environment and tree species.
2. The nesting height does not significantly depend on the habitat itself or on the tree species.
Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.
The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yi...eld and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NO3¯N were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).
Laboratory methods of semen evaluation are used to select males for artificial insemination. The current review describes several techniques that have been recently used for sperm analysis. Conventional microscopic methods in combination with the objective computerassisted sperm motility and morphology analyzers and flow cytometry, allows to ob...tain more precise information about the membrane and functional status of spermatozoa. By using several methods to detect motility, viability, acrosomal and capacitation status besides DNA integrity sperm biology and some of the mechanism involved in sperm cry injury can be better understood. The number of possible targets related to sperm quality is increasing, and possible that some of them could enable sperm analysis for predicting freezability and fertility to be improved.
From a nutritional point of view, sheep milk is more valuable than cow and goat milk and the interest for sheep milk is increasing in many countries. However, sheep milk is easily contaminated during milking, handling, and transport and it is an ideal medium for bacterial propagation. Consequently, sheep milk spoils quite quickly. The proper, c...lean handling of milk is not only of sanitarian interest, but it also serves the farmers’ interests, because contaminated milk may not be distributed, and is unsuitable for producing good quality products. Following this technological trend, this review addresses the bacterial composition of sheep milk with and without mastitis. Even though sheep milk contains a lot of bacteria, this review article highlighted total plate count, Enterobacteriaceae, coliform, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Campylobacter, Salmonella spp. and Streptococcus spp. Mastitis in sheep is a vital cause of mortality, reduction in milk production and early culling. The reported risk factors for mastitis in sheep were age, a case of mastitis, breed, husbandry systems, and location. The main priority should be implementation of programs to minimize human pathogenic bacteria and mastitis in raw ewe milk.
Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression... from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments.
The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obviou...s results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum... basilicum).
Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.
In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments.
Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated... water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
The biggest problem of Hungarian crop farming is mass production and the simple crop rotation based on cereals. There was a decrease in sowing area of protein crops which raises crucial issues in crop rotation and land use. Therefore, growing papilionaceous plants, which are now considered to be alternative plants, should be taken under clo...se examination. Hairy vetch (Vicia villosa Roth.) belongs to the family of papilionaceous plants and it can be grown in light weak soils.
In Hungary, hairy vetch was used as green forage at first, but it later became a green manure plant. Nowadays, it is used as a cover crop and its sowing seed has a good export market. In low fertile soils it is able to produce a big amount of green yield (25–40 t ha-1) even in spring while its seed yield could be 0.4–0.5 t ha-1 at farm level. In addition to its morphological characteristics hairy vetch is grown mainly with a supporting plant, i.e. triticale in many cases.
Our purpose was to test the harvest index and its agrotechnical and botanical factors of hairy vetch in different cropping systems.
Point sampling, which is also known as angle-count sampling (ACS), can be considered an efficient way of estimating the basal area and volume of forest stands. It is possible to use it in forest management: providing more accurate estimates (precision <10%) of site and stand characteristics needed for management planning. 20 black locu...st (Robinina pseudoacacia L.) stands were selected at final cutting age to determine the regeneration criteria based on their total volume. It was verified that at P=5% there was no difference between the main volume values of stands indicated in the relevant forest plans as well as calculated by the ACS method.
Protection of natural resources and sustainable natural resources management are essential for the long-term survival of humanity. This makes necessary nowadays the development of environmentally conscious living and spread of that in the future. The amount of organic waste materials, produced during human activities, could be decreased by comp...osting instead of dispose them in landfills. Applying appropriate treatment technology and additives, the compost could be used as fertilizer for horticultural crops and it could increase the easily available nutrient content of soils. Compost utilization prevents nutrient deficiencies and by using the optimal rate, we could reach significant yield increases.
In Hungary, black locust (Robinia pseudoacacia L.) is considered as an important exotic stand-forming tree species growing mostly under unfavourable ecological conditions for forest management. Due to climate change effects, its importance is increasing in many other countries, too. As a result of a selection programme, new black locust clones...were tested in clone trials. Juvenile growth of 12 micropropagated black locust clones in two plots series established at different dates were evaluated in central Hungary under marginal site conditions. At age of 7 the clone R. p. ‘Bácska’ (‘KH 56A2/5’), at age of 10 the clones R.p. ‘Homoki’ (‘MB17D3/4’) and ‘PV201E2/4’ appeared to be especially promising for mass production. Based on the data obtained from the performed trials, it can also be concluded that tissue culture can be considered as a suitable tool for propagating superior individuals and offers new prospects for the rapid cloning of selected genotypes used for plantation forestry.
Grapevine trunk diseases (GTDs) are among the most severe problems in viticulture worldwide. The exact etiology and the role of endophytic microorganisms is not known yet and there is no adequate protection or curative treatment against the disease. Hungarian wine regions are also affected by the disease, and there is restricted information... about the rate of infection nation-wide and about the susceptibility of the Hungarian cultivars.
The main objectives of our research are to measure the symptom expression and the damage caused by GTDs, to understand the epidemiology and etiology of the disease to establish a foundation of a proper disease management.
Cultivar susceptibility groups were created with the aim to allocate some Hungarian cultivars and the role of vineyard age was also examined in symptom expression.
The goal of research planning is to optimize human and material resources while maximizing efficiency. If there are databases that can be used as a substitute for own data collection, effective research can be facilitated by reallocating resources. In the case of environmental studies, the knowledge of the climatic conditions of the study p...eriod is known to be a key factor in research planning process.
In the present study, the data of our meteorological station in our research area (known as “Szamárháti Tanya”, Kesznyéten, Hungary) was compared with the measurements of meteorological stations operated by the competent water authority. Stations were taken into the study within a 10 and 20 km radius over a period of 21 months, to determine which provided more relevant data from the area.
During the evaluation of results, the relationship between the local and regional weather stations were determined, and deductions were made from the obtained results to support the decision which of the targeted investigations could be of greater benefit.
In the growing season of 2019, we analysed stress resulting from climatic factors on maize hybrids of different genotypes, with the aim of gaining a better understanding of the physiological responses of each hybrid, which might support the elaboration of a cost-effective irrigation plan.
Our experiments were carried out at... the Látókép Experimental Station of the University of Debrecen on calcareous chernozem soil in a small-plot long-term field trial with strip plot design. In the scope of the experiment, N-fertilizer doses were applied as basic fertilizer and top-dressing in addition to the non-fertilized (control) treatment. The 60 and 120 kg N/ha doses applied as basic fertilizers in the spring were followed by top-dressing in the V6 phenophase with a +30 kg N/ha dose. Measurements were carried out with the involvement of the Renfor early (FAO 320) and Fornad (FAO 420) late maturity hybrids-
The stomata of the plants became more and more closed with the progression of the phenological phases; their stomatal conductance decreased. However, the hybrids responded differently to environmental stress. In the case of the Renfor hybrid, the highest conductance (669 mmol/m2-s) was recorded in the V12 phenophase with the 150 kg N/ha treatment. The stomata were more open due to the high turgor pressure, allowing plants to evaporate properly. The plant was in its worst physiological condition on 2nd July, at the time of the appearance of the last leaf in the case of the 120 kg N dose (224 mmol/m2-s). The value measured in the V12 phenophase has already shown that the stomata were closing due to the self-regulating system of the plant. It would have been necessary to dispense irrigation water following the measurement. This confirms the finding that water stress can be prevented by measuring stomatal conductance.
In the case of the Fornad hybrid, stomatal conductance was the highest on 12th June (630 mmol/m2-s) in the 90 kg N/ha treatment and it was the lowest (183 mmol/m2-s) in VT (emergence of the last leaf) phenophase in the 60 kg N/ha treatment. In this case, the appropriate time for applying irrigation water would have been early July, when the conditions for the plants were still adequate. Subsequently, the stomata began to close due to a reduction of the water resources available to them.
There was a significant correlation between soil moisture and stomatal conductance, as well as between temperature and stomatal conductance.
Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland... vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
Host-specificity is an important characteristic of fungal pathogens. Changing climate could create more appropriate environmental conditions for phytopathogens, thus formerly host-specify fungi could be able to colonize new hosts. Noxious plant pathogen fungi, which can infect several plant species are well-known worldwide. These genera may... expand their range of hosts because of the appearance in new geographic areas due to climate change. This new exposure can result in serious problems in agriculture because of the lack of immunity. The susceptibility of apple tree was studied through testing pathogenicity in vitro with species isolated from walnut twigs and nuts, and identified by ITS sequences. Three of four tested species, Botryosphaeria dothidea, Diaporthe eres and Diplodia seriata colonized and necrotized the infected apple branches, while Juglanconis juglandina was not able to infect the twigs. Members of Botryosphaeriaceae were the most virulent, causing the largest lesions in the fastest way. This experiment draws attention to the threat of new host-pathogen connections, which can arise because of the favourable weather conditions and can spread between neighbouring cultures.
The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in u...ndeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.