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Impact of chronic heat stress on digestibility of nutrients and performance of meat type ducks
51-55Views:385The aim of the study is to determine the effect of vitamin and mineral supplement under permanently high environmental temperature (30±1 °C) on the digestibility of nutrients, performance and furthermore the composition of duck meat in the growing period. A total of four hundred mixed sex 14 days old Cherry Valley type hybrid ducks were used for the study. Two experimental diets were formulated in the study (control and vitamin E, C and zinc supplemented diet). Based on the results the following conclusions were drown: the antioxidant defence system plays an important role in the reduction of heat stress generated lipid peroxidation process. Feed additives which have direct or indirect antioxidant effects can reduce the negative effects of heat stress on the ducks performance and meat composition. Digestibility of nutrients (Dry Matter, Crude Protein, Crude Fat) was not affected by antioxidant supplementation under chronic heat stress (30±1 °C). The performance was affected significantly by Vitamin C and E and zinc supplementation under heat stress (P<0.05). In the treated group the daily weight gain (dWG) increased and the feed conversion ratio (FCR) was improved significantly (P<0.05). The energy and protein conversion was decreased also significantly (P<0.05).
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Evaluation of the correlation between SPAD readings and absolute chlorophyll content of maize under different nitrogen supply conditions
121-126Views:915Currently, one of the most important objectives of agriculture is to maintain the principles of the sustainability. The use of precision technologies in agriculture belongs to this topic. The use of precision technologies is increasingly widespread in the cultivation of various agricultural crops, including maize. Sensing is an important part of these techniques. In our experiment we compared two methods: measuring relative chlorophyll content and the method of determine the extractable chlorophylls. The experimental plant was maize (Zea mays L.) and the measurements were performed at an early development stage (V8) of three genotypes. Three levels of nitrogen (0; 80; 160 kg ha-1) were applied during the experiment. The relative chlorophyll content was measured by SPAD-502 (Minolta, Japan) and a handheld GreenSeeker (Trimble, USA) device. The extractable total chlorophyll content decreased in parallel with the increased nitrogen level. The obtained SPAD values were diversified furthermore the NDVI values have not been changed for the effect of different nitrogen fertilization. In the early stages of development of maize, these parameters need to be complemented with other measurements to provide reliable information about the crops nitrogen status.
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Daily behaviour of Hungarian Grey Cattle under range grazing conditions
45-48Views:396Cattle behaviour on rangeland depends on external factors, such as grass allowance and quality, temperature, net solar radiation, distance from water-source, wind speed and direction, air pressure changes and the applied breeding technology. Our research is based on previous empirical observations and modern methods to analyze the behaviour of the Hungarian Grey Cattle. Today it is crucial to use cost-effective solutions in modern beef cattle farming therefore we introduce a cost-efficient method to study and follow cattle herds. We are studying relation between traveled daily distance and air pressure. The various weather fronts influence behavioural characteristics and traveled daily distance. According to our hypothesis, the pressure-change and the wind direction has significant effect on cattle activity on pasture. As the different air masses alter the barometric conditions and unbalance the neuroendocrine system, indirectly cause relaxed or agitated behaviour
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Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)
127-131Views:776Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and yield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:503Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:364Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Leaf reflectance characteristics and yield of spring oat varieties as influenced by varietal divergences and nutritional supply
29-34Views:306Inadequacy of nutrients in the soil and sufficient inaccessibility to nutrients is caused by factors that affect production and productivity of spring oat varieties. Exogenous application of nutrient and real time nutrient assessment can therefore reverse these associated negative consequences. A field experiment was conducted to evaluate the response of spring oat varieties to sulphur and silicon based fertilisation. Eight spring oat varieties, four level of nutrient application was arranged in a split-plot design with three replications. The obtained results showed that foliar application of sulphur improves the grain yield of most nutrients responsive varieties by about 34.7%. However, application of silicon had shown a diminishing return association to grain yield of variety GK Kormorán, GK Pillangó, Lota, Panni. LAI, thousands grain weight, SPAD, NDVI was significantly (p < 0.05) influenced by genetic difference of the tested varieties, developmental plasticity, and nutrient application. Significantly higher grain yield was obtained from the variety Mv Pehely than the other tested varieties. Therefore, it could be inferred that a combined use of nutrient responsive spring oat varieties and sulphur containing fertilisers could be important agronomic practice to improve grain yield and to develop climate resilient oat varieties.
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Evaluation of water balance in apple and pear trees
193-198Views:683A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:582An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:373The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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Evaluation of population parameters and biometric data of an Eurasian Collared Dove (Streptopelia decaocto Friv.) population in the Great Plain of Hungary
89-92Views:625The Eurasian Collared Dove shows different patterns in density all over Hungary. In some cases, the population has decreased to a lower level, but there are habitats where the population remained stable. Several factors were taken into consideration during the evaluation of this phenomenon. These publications state that the increased urbanization of corvids, the lack of food sources, and the changes in nesting sites caused the decrease in numbers. During our research, we intend to contrast the differences in population structure of Collared Doves that differ from each other in the density of predators presented at nesting sites. As a first step, in this paper, numerous samples (n=118) were taken from a population that is less influenced by corvid species’ nest predation. Our prediction was that the population parameters remained at the level of 1980’s, in which the population of Eurasian Collared Dove has reached its maximum, according to further publications.
During the evaluation process, our data were collected from hunting bags near Nyíregyháza. Results were compared with former publications. We stated that the population structure, such as age and gender distribution remained stable in the evaluated population. In addition, morphometric factors of the collected individuals were also measured, such as weight, wing length, 3rd primary feather, and tail length, which were compared with former surveys. During statistical tests no significant difference was revealed among population parameters, but there were significant differences between most of the morphometric data, (p>0.05).
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Data on the bumblebee assemblages (Apidae: Bombus spp.) lives in lands under agri-environment commitment
31-35Views:510The goal of agri-environmental schemes (AES) and greening programs are protecting and increasing biodiversity in agricultural lands. The evaluation of effectiveness of AES needs further investigations. For the purpose of investigations, species and species groups should be selected which can indicate the effects of changes in landscape use on biodiversity. Bumblebees are good indicators for this purpose.
The role of bumblebees in pollination is well studied but in the case of different crops, much less detailed data are available. In 2018, bumblebee assemblages of 44 sites belonged to 8 different agricultural and semi-natural habitat types were studied in the surroundings of Sajószöged, Tiszaújváros and Derecske.
This study provides new distribution data of 8 bumblebee species in three 10×10 km UTM cells covering the sampling area. According to our results, the alfalfa and red clover fields and semi-natural grasslands has more species rich and abundant bumblebee assemblages than different crop fields (sunflower, oilseed radish and vegetable morrow) and can help protect bumblebee assemblages of agricultural lands. Based on the collected distribution and abundance data, the role of the bumblebees in pollination of the studied crops should be re-evaluated.
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Promising Leuce poplar clones in sandy ridges between the rivers Danube and Tisza in Hungary: a case study
111-113Views:493An intensive integrated research and development work has been carried out on the improvement of Leuce poplars including primarily the native white poplar (Populus alba L.) and its natural hybrid grey poplar (Populus × canescens). More than 70 percent of the Leuce poplar stands can be found on calcareous sandy sites in the Danube–Tisza region, so they play a significant role in the poplar management of this part of the country. The most important task ahead of Hungarian poplar growers is to improve the quality of poplar stands and plantations based on selecting new clones and cultivars. The growth and yield of four promising Leuce poplar clones was evaluated on a marginal site in central Hungary. The clones ‘H 425-4’ (Populus alba L. × Populus alba L.), and ‘H 758’ (Populus alba L. Mosonmagyaróvár 124) seem to be suitable for wood production, while the ‘H 427-3’ (Populus alba L. × Populus alba L. cv. Bolleana) and ‘H 422-9’ (Populus alba L. × Populus grandidentata (Michx)) clones (with decorative stem form) could be better used for tree lines and ornamental plantations.
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Investment funding programs on dairy farms in Hajdú-Bihar County
11-16Views:314The dairy farming sector needs constant modernization of its buildings, machines and technology in order to maintain its competitiveness and productivity. For the greater scale investments, these companies try to apply for state funds in addition to their own financial sources. Therefore, the investment support system plays a great role by improving the competitiveness and modernity of these farms. In the last 10 years, 7 funding programs were available for dairy farms. In this research, 25 dairy farms were interviewed in Hajdú-Bihar County. The examined farms cover more than 50% of the milk production in Hajdú-Bihar County. The survey questions focused on the investment funding program use of farms between 2007 and 2017 and renewable energy investments including all programs. The results show how many tenders were submitted, how many were successfully evaluated and completed. All farms had at least two successfully evaluated projects. A correlation was found between completed projects by farm size and completed projects. The connection of the modernity index of dairy farms and the completed projects was also shown.
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Classification of a diffuse heavy metal polluted mining site using a spectral angle mapper
119-123Views:922Characterization of heavy metal polluted abandoned mining sites is complicated, as the spatial distribution of pollutants often changes dramatically.
In our study, a hyperspectral data analysis of the Gyöngyösoroszi abandoned Pb-Zn mine, located in northern Hungary, where Záray (1991) reported serious heavy metal contamination, was carried out using ENVI 4.3. In this area, galena (PbS), goethite (FeO(OH)), jarosite (KFe3(SO4)2(OH)6), sphalerite ((Zn, Fe)S) and pyrite (FeS2) were the predominant minerals in the alteration zones was chosen as the target mineral.
Spectral angle mapper (SAM) and BandMax classification techniques were applied to obtain rule mineral images. Each pixel in these rule images represents the similarity between the corresponding pixels in the hyperspectral image to a reference spectrum.
As a result of hyperspectral imagery the distribution of pyritic minerals (sphalerite, galena) in the area was defined. Both of the mineral formations occur, especially in mine tailings, the area of the ore preparatory, and the Szárazvölgyi flotation sludge reservoir. According to the results, jarosite and goethite have similar distributions to sphalerite and galena. The results showed that hyperspectral remote sensing is an effective tool for the
characterization of Pb, Zn and Fe containing minerals at the examined polluted sites and for modelling the distribution of heavy metals and minerals in extensive areas.
This classification method is a basis of further detailed investigations, based on field measurements, to map the heavy metal distribution of the studied area and to quantify the environmental risks caused by erosion, which include DEM (digital elevation model) and climatic and hydrological data sources. Furthermore, it can be used primarily to support the potentially applicable phytostabilization technique and to isolate hot spots where only ex-situ remediation techniques can be applied. -
The effect of crop protection and agrotechnical factors on sunflower in the Hajdúság region
39-46Views:296Extreme weather conditions are becoming more and more frequent in the crop years, thus increase the risk of sunflower production.
The objective of researches into plant production is to minimize these effects as much as possible. In this sense, the optimization of
agrotechnological factors is of high importance. Within these factors, the appropriate crop technology (sowing time, crop density)
and optimized, rational crop protection technologies are important, especially in the highly sensitive sunflower cultures. The effect of
sowing time, crop density, and fungicide treatments on the yield of sunflower hybrids was analysed in different crop years in 2008
and 2009. In each case, the infection was highest with the early sowing time and at the highest crop density level (65000 ha-1). When
one fungicide treatment was applied, the rate of infection decreased compared to the control treatment. The further decrease of the
infection rate was less after the second fungicide treatment.
In the humid year of 2008 the crop yield was the highest at 45000 ha-1 crop density level in the control treatment and at 55000 crop
ha-1 crop density level when fungicides were applied. In the draughty year of 2009 the maximum yield was gained at 55000 ha-1 crop
density level in the control treatment and at 65000 crop ha-1 when fungicides were applied. In 2008 and 2009 as regards the crop
yield, the difference between the optimal and minimal crop density levels was higher in the fungicide treatments than in the control
treatment (in 2008: control: 517 kg ha-1; one application of fungicides: 865 kg ha-1; two applications of fungicides: 842 kg ha-1), (in
2009: control: 577 kg ha-1; one application of fungicides: 761 kg ha-1; two applications of fungicides: 905 kg ha-1).
In each and every case, the first treatment with fungicides was more effective than the second. In 2008, the highest yield was
obtained with the third, late sowing time in each fungicide treatment. The differences between the crop yields with different sowing
times was less than in 2009, when the results of the second treatment exceeded those of the first and third treatment in each case. -
Effect of the media on morphology of Cryphonectria parasitica (Murr.) Barr isolates and their Vegetative Compatibility Groups
38-42Views:527The most dangerous pathogen for the European and American chestnuts is the blight fungus Cryphonectria parasitica (Murrill) Barr. Short after its introduction a big number of chestnut trees were destroyed on the infested area. The control could be really complicated, because of the numerous vegetative compatibility groups of the fungus. There is a type that carries a mycovirus viz. hypovirus in the cytoplasm. We are able to control effectively this pathogen by using mycovirus-carrying strains (called hypovirulent fungal strains also). In laboratory it is easy to multiply the virulent and the hypovirulent strains of the fungus but do not easy to differentiate colonies visually on simple PDA medium. During our research, we tested different types of media, based on potato and chestnut bark extract respectively. It was observed that on potato medium the virulent strains produce more orange pigments. So it is more easy to differentiate virulent or hypovirulent isolates of chestnut blight fungus based on colony colour and morphology.
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Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:290The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Comparative study of a winter wheat variety and hybrid sown after different pre-crops on chernozem soil
63-69Views:549Wheat production is a determining branch within Hungarian crop production (produced on nearly one million hectares). Weather anomalies caused by climatic change confirmed the importance of the biological background (variety, hybrid) in wheat production. The adapting ability and reaction of different wheat genotypes towards nutrient supply were studied in a long-term field experiment on chernozem soil type in the case of different pre-crops (sunflower and maize). According to the experimental results of the vegetation of 2017/2018, the yield of the variety Ingenio sown after the sunflower as previous crop ranged between 4168 and 8734 kg ha-1, while in the case of maize as previous crop, this value ranged between 2084 and 7782kg ha-1, depending on the applied nutrient supply level. The studied genotypes produced rather significant yield surplus as a response to the application of mineral fertilization (4.6–5.1 t ha-1 after sunflower and 5.7–6.3 t ha-1 after maize). Optimal mineral fertilizer dosage was determined by both the genotype and the pre-crop. N-optimum values of wheat genotypes was determined using regression analysis. In the case of the variety Ingenio sown after sunflower, the optimum range was N144-150+PK, while after maize, it was
N123-150+PK, respectively. For the hybrid Hyland, these optimum ranges were N114-120+PK, just as N150-153+PK, resp. The application of optimal mineral fertilizer dosages improved water utilization of the studied wheat genotypes to a significant extent. WUE values of the control, unfertilized treatments ranged between 4.1–8.3 kg mm-1, while in optimal fertilizer treatment, it ranged between 15.5 and 17.4 kg mm-1. -
Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:636The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:286Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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Analysis of different fertilization settings’ effect in the case of the summer savory’s (Satureja hortensis L.) yield and active agents
101-105Views:324During our research we investigated the Mediterranean summer savory’s (Satureja hortensis L.) nutrient requirement with different fertilization settings in a small plot trial. We measured the medicinal plant’s raw and dry drug mass, and the presence of the main essential oil active agents under the different fertilization settings using SPME (Solid phase microextraction) and GC-MS (Gas Chromatograph mass spectrometer).From the drug’s raw and dry mass perspective, based on the obtained results, in our opinion, the N30P40K60 nutrient requirements were ideal in 2015, while the N60P80K120 nutrient requirement showed the best values in 2016. A significant relationship is assumed between the different fertilization settings and the essential oil active agents’ distribution. During the analysis of data, we compared three of the savory’s essential oil active agents’ percentage of presence in the nutrient requirement samples. -
Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:645Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:523Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Evaluation of technologies and resources from the spatial and social aspect
73-75Views:562The basic functioning of the world is mostly led by negative trends. One of the fundamental reasons for this phenomenon is that the different technologies (hardware, software, resources) do not act properly and in the proper structure by means of the produced products and services. Therefore, the examination of technologies and resources is a key question of our world and development from various aspects. Technologies convey all positive and negative impacts which can be regarded as the fundamental reasons and causes of harmonious or disharmonious development. For this reason, the role of technologies in development and harmonious functioning is of paramount importance.
As a final conclusion, we established that the different technologies (hardware, software, resources) are interpreted as impact conveyors which predominate by means of products and services in a way that their relation system is determined. By using the thermodynamic interpretation which is the basis of the functioning of the world, we built a system consisting of 14 elements to evaluate different technologies, to interpret its functioning and to develop positive interactions and trends.