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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:244Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:179Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Evaluation of technologies and resources from the spatial and social aspect
73-75Views:175The basic functioning of the world is mostly led by negative trends. One of the fundamental reasons for this phenomenon is that the different technologies (hardware, software, resources) do not act properly and in the proper structure by means of the produced products and services. Therefore, the examination of technologies and resources is a key question of our world and development from various aspects. Technologies convey all positive and negative impacts which can be regarded as the fundamental reasons and causes of harmonious or disharmonious development. For this reason, the role of technologies in development and harmonious functioning is of paramount importance.
As a final conclusion, we established that the different technologies (hardware, software, resources) are interpreted as impact conveyors which predominate by means of products and services in a way that their relation system is determined. By using the thermodynamic interpretation which is the basis of the functioning of the world, we built a system consisting of 14 elements to evaluate different technologies, to interpret its functioning and to develop positive interactions and trends.
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White rust species (Chromista, Peronosporomycetes, Albuginales, Albuginaceae) on common weeds in Hungary
30-33Views:206The obligate plant parasite fungi in the family Albuginaceae are responsible for causing white rust diseases on weeds and they are rather common worldwide. Weedy plants with characteristic symptoms have been collected in 2014 and 2015 on location Hajdú-Bihar and Jász-Nagykun-Szolnok counties in Hungary. The determination of the species were based on the morphological characters both pathogens and hosts. Albugo candida was determined on shepherd’s purse (Capsella bursa-pastoris). Common purslane (Portulaca oleracea) is a host for Wilsoniana portulacae. The fungus Wilsonia bliti (syn.: Albugo bliti), the causal agent of white rust disease was found on redroot pigweed (Amaranthus retroflexus).
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Impact of nitrogen and sulphur fertilization on the growth and micronutrient content of spring wheat (Triticum aestivum L.)
211-219Views:166Micronutrients are as important as macronutrients for crops. Each micronutrient has its own function in plant growth. Zinc is important for membrane integrity and phytochrome activities. Copper is an essential micronutrient required for the growth of wheat. Manganese is required for enzyme activation, in electron transport, and in disease resistance. The pot experiment was set up in greenhouse on calcareous chernozem soil Debrecen-Látókép with a spring wheat. In certain development stages (according to BBCH growth scale of wheat), at the beginning of stem elongation (29–30), at the heading (51–59), at the flowering (61–69) stage three average plants were removed from all pots for analysis. Fresh and dry weight of the plant samples were measured. Plant leaves after drying were digested by HNO3-H2O2 methods and manganese, zinc and copper contents of plant were quantified by atomic absorption spectrophotometry. At the flowering stage, when the nutrient uptake of plants is the most intensive, the weight of wheat ranged between 0.94–1.57 g plant-1. In this development stage, the NS2 treatment produced the highest weight of wheat, and compared to this the NS3 treatment decreased that value already. The results show unfavourable effect of NS3 treatment. On the basis of microelement content of wheat and the weight of a plant, nutrient uptake by plant were calculated. At the beginning of growth the starter treatment had positive effect on Cu-uptake compared to the NS1 treatment, where the same dose of fertilizer was stirred into the soil. Wheat is very sensitive to copper deficiency, so copper dissolved by starter treatment could be favourable to the early development of wheat. At flowering stage the Zn-uptake of wheat became the highest and it was between 133.7–234.6 mg plant-1. The Mn-uptake of wheat plant was higher than the Cu- and Zn-uptake of wheat.
This phenomenon can be explained by the fact that the untreated soil had higher Mn-content, than Cu- and Zn-content. To summarize the results, it can be stated, that the copper uptake of wheat was more affected by the different treatments in the stage of stem elongation, while Mn- and Zn-uptake of wheat were influenced primarily in the stage of heading and flowering.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:91Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Leaf reflectance characteristics and yield of spring oat varieties as influenced by varietal divergences and nutritional supply
29-34Views:89Inadequacy of nutrients in the soil and sufficient inaccessibility to nutrients is caused by factors that affect production and productivity of spring oat varieties. Exogenous application of nutrient and real time nutrient assessment can therefore reverse these associated negative consequences. A field experiment was conducted to evaluate the response of spring oat varieties to sulphur and silicon based fertilisation. Eight spring oat varieties, four level of nutrient application was arranged in a split-plot design with three replications. The obtained results showed that foliar application of sulphur improves the grain yield of most nutrients responsive varieties by about 34.7%. However, application of silicon had shown a diminishing return association to grain yield of variety GK Kormorán, GK Pillangó, Lota, Panni. LAI, thousands grain weight, SPAD, NDVI was significantly (p < 0.05) influenced by genetic difference of the tested varieties, developmental plasticity, and nutrient application. Significantly higher grain yield was obtained from the variety Mv Pehely than the other tested varieties. Therefore, it could be inferred that a combined use of nutrient responsive spring oat varieties and sulphur containing fertilisers could be important agronomic practice to improve grain yield and to develop climate resilient oat varieties.
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Evaluation of water balance in apple and pear trees
193-198Views:102A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:227An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
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Data on the bumblebee assemblages (Apidae: Bombus spp.) lives in lands under agri-environment commitment
31-35Views:199The goal of agri-environmental schemes (AES) and greening programs are protecting and increasing biodiversity in agricultural lands. The evaluation of effectiveness of AES needs further investigations. For the purpose of investigations, species and species groups should be selected which can indicate the effects of changes in landscape use on biodiversity. Bumblebees are good indicators for this purpose.
The role of bumblebees in pollination is well studied but in the case of different crops, much less detailed data are available. In 2018, bumblebee assemblages of 44 sites belonged to 8 different agricultural and semi-natural habitat types were studied in the surroundings of Sajószöged, Tiszaújváros and Derecske.
This study provides new distribution data of 8 bumblebee species in three 10×10 km UTM cells covering the sampling area. According to our results, the alfalfa and red clover fields and semi-natural grasslands has more species rich and abundant bumblebee assemblages than different crop fields (sunflower, oilseed radish and vegetable morrow) and can help protect bumblebee assemblages of agricultural lands. Based on the collected distribution and abundance data, the role of the bumblebees in pollination of the studied crops should be re-evaluated.
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The effect of crop protection and agrotechnical factors on sunflower in the Hajdúság region
39-46Views:79Extreme weather conditions are becoming more and more frequent in the crop years, thus increase the risk of sunflower production.
The objective of researches into plant production is to minimize these effects as much as possible. In this sense, the optimization of
agrotechnological factors is of high importance. Within these factors, the appropriate crop technology (sowing time, crop density)
and optimized, rational crop protection technologies are important, especially in the highly sensitive sunflower cultures. The effect of
sowing time, crop density, and fungicide treatments on the yield of sunflower hybrids was analysed in different crop years in 2008
and 2009. In each case, the infection was highest with the early sowing time and at the highest crop density level (65000 ha-1). When
one fungicide treatment was applied, the rate of infection decreased compared to the control treatment. The further decrease of the
infection rate was less after the second fungicide treatment.
In the humid year of 2008 the crop yield was the highest at 45000 ha-1 crop density level in the control treatment and at 55000 crop
ha-1 crop density level when fungicides were applied. In the draughty year of 2009 the maximum yield was gained at 55000 ha-1 crop
density level in the control treatment and at 65000 crop ha-1 when fungicides were applied. In 2008 and 2009 as regards the crop
yield, the difference between the optimal and minimal crop density levels was higher in the fungicide treatments than in the control
treatment (in 2008: control: 517 kg ha-1; one application of fungicides: 865 kg ha-1; two applications of fungicides: 842 kg ha-1), (in
2009: control: 577 kg ha-1; one application of fungicides: 761 kg ha-1; two applications of fungicides: 905 kg ha-1).
In each and every case, the first treatment with fungicides was more effective than the second. In 2008, the highest yield was
obtained with the third, late sowing time in each fungicide treatment. The differences between the crop yields with different sowing
times was less than in 2009, when the results of the second treatment exceeded those of the first and third treatment in each case. -
Nitrogen Supplying Capacity of Brown Forest Soil under Different Cropping Practices and 0.01 M CaCl2 Soluble Organic Nitrogen
17-23Views:86The best known and most remarkable example of continuous production in Hungary is the Westsik’s crop rotation experiment, which was established in 1929, and is still in use to study the effects of organic manure treatment, to develop models, and predict the likely effects of different cropping systems on soil properties and crop yields. In this respect, Westsik’s crop rotation experiment provides data of immediate value to farmers concerning the applications of green, straw and farmyard manure, as well as data sets for scientific research.
Although commonly ignored, the release of nitrogen by root and green manure crops has a significant impact on soil organic matter turnover. The design of sustainable nitrogen management strategies requires a better understanding of the processes influencing nitrogen supplying capacity, as the effects of soil organic matter on soil productivity and crop yield are still very uncertain and require further research. In the treatments of Westsik’s crop rotation experiment, nutrients removed from soil through plant growth and harvesting are replaced either by fertilisers and/or organic manure. Data can be used to study the nitrogen supplying capacity of soil under different cropping systems and its effect on the 0.01 M CaCl2 soluble organic nitrogen content of soil.
The aim of this paper is to present data on the nitrogen supplying capacity of brown forest soil from Westsik’s crop rotation experiment and to study its correlation with hundredth molar calcium-chloride soluble organic nitrogen. The main objective is to determine the effects of root and green manure crops on the nitrogen supplying capacity of soil under different cropping systems. The nitrogen supplying capacity was calculated as a difference of plant uptake, organic manure and fertiliser supply.
The 0.01 M CaCl2 soluble organic nitrogen test has proved reliable for determining the nitrogen supplying capacity of soils. Brown forest soils are low in organic matter and in the F-1 fallow-rye-potato rotation, the nitrogen supplying capacity was 15.6 kg/ha/year. 0.01 M CaCl2 soluble organic nitrogen content was as low as 1.73 mg/kg soil. Roots and green manure increased the nitrogen supplying capacity of soil by more than 100%. This increase is caused by lupine, a legumes crop, which is very well adapted to the acidic soil conditions of the Nyírség region, and cultivated as a green or root manure crop to increase soil fertility. -
Leaf and root evaluation of bioactive compounds of different beetroot varieties
135-139Views:195The importance of beetroot lies in the numerous health benefits, attributed to its wide-ranging array of minerals, vitamins and bioactive compounds which play a key role in the human diet. Generally, beetroot is consumed for its root, however, its leaves also contain a good amount of bioactive compounds. The experiment was carried out with four beetroot varieties in order to compare the amount of bioactive compounds in the root and the leaf. The analyzed data were total dry matter content, total polyphenol, flavonoid and vitamin C.Results have shown that roots contain the highest amount of bioactive compounds. Nevertheless, leaves can be also considered a significant source. Therefore, consumption of the young leaves is highly recommended due to their availability after thinning or at maturity stage (60 days after the germination). Of the examined varieties, ‘Libero’ demonstrated the highest total polyphenol, flavonoid and vitamin C values, although it was found to be statistically insignificant. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:72The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Evaluation of mineral element content of beetroot during the different stages of the growing season
459-469Views:249In modern nutrition, bioactive materials of different vegetables are especially important to be researched. The experiment was carried out on March 30, 2016 (sowing date). Mineral element content (B, Ca, Cu, Fe, K, Mg, Na, P, S, Zn) was evaluated on the 60th, 85th and the 105th day of the vegetation period. The data are presented as the mean of five varieties which can give the real state of these parameters of beetroot grown on lowland chernozem soil.
In the young beetroot leaves (60 days) the mineral element content was higher than in the older ones (85 days). The calcium and magnesium content of the leaves was much more favourable (10 times higher) than in the root. The potassium content of leaves has reached the amount of 5000 mg kg-1, but at the same time more than 3000 mg kg-1 was detected in the improved root. This value is favourable for the potassium supply of the human organism.
The sulphur content (1300 mg kg-1) of the leaves was the highest on the 85th day of vegetation period. Similar tendency was detected for boron content (2.45 mg kg-1), while for iron content it was higher (28.23 mg kg-1) in the younger leaves (60 days).
Finally, it can be concluded that the increased element content of beetroot leaves will be a favourable source of mineral element supply of the human organism.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:215The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Basic Research for the Development of Fertiliser Spreaders
52-57Views:79The knowledge of the physical characteristics of fertiliser particles is essential for the constructors and operators of fertiliser distributors. Among physical characteristics, the most important are the frictional and aerodynamic properties for the description of particle movement. Adjustable angled slopes, shearing boxes and various rotating disks are used to identify frictional properties. We have developed a high precision shearing box with digital force measuring cells and a distance signaller (incremental transducer) that we use for slide tests efficiently. We measured the frictional characteristics of 6 different fertilisers: the inner coefficient of friction and the coefficient of friction on ten test surfaces most commonly used in machinery, and we specified the relationship between displacement, loading and the coefficient of friction. We can conclude that the material of the frictional surface significantly influences the force of friction.
However, our experience tells us that the shearing box is not suitable for the measurement of the inner friction, since the examined particles slide on the metal surface of the shearing box in a growing extent in the course of displacement, so it does not measure the real inner friction. Therefore, in our experiment we have developed rotating shearing equipment with a constant shearing surface to identify the inner friction. We tested the equipment with fertilisers and we identified the inner frictional characteristics of 6 different fertilisers. With the developed rotating shearing apparatus we could measure the real inner friction of the particles.
To identify the aerodynamic characteristics of granules, wind tunnels and free-fall tests are used. An elutriator have been developed for our investigation. We have used fertilisers for testing the measuring equipment and we have identified the aerodynamic characteristics of 6 different fertilisers. -
Correlation between the weather in 2017 and the productivity of maize
89-93Views:152In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize. The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2; therefore, this experiment was half-industrial. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. The yield increasing effect of the fertilizer also depended on the number of plants per hectare to a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants ha-1.In Hajdúszoboszló in 2017, up to October, 445.8 mm of rain fell, which is in line with the average values of 30 years, and is only 45.7 mm less than those. In 2017, the effect of increasing the plant number was slighter. Averaged over the observed fertilizer treatments and hybrids, the yield was 9.10 t ha-1 with 60 thousand plants ha-1, 9.11 t ha-1 with 70 thousand plants ha-1 and 9.12 t ha-1 with 80 thousand plants ha-1. Without fertilization, in most cases, increasing the plant number from 60 thousand plants ha-1 to 70-80 thousand plants ha-1 does not increased the yield but decreased it. With N80+PK treatment the yield changed between 8.90 and 11.27 t ha-1. The effect of increasing the plant number was just slightly observable and did not show a clear tendency. The effect of changing the plant number, even with the highest dosage of fertilizers, could not be detected adequately. In contrast with the plant number, the effect of the different fertilizer treatments was expressly traceable. Compared to the control treatment (treatment without fertilization), with N80+PK fertilizer dosage with 60 thousand plants ha-1 the yield increased by 3.36–4.99 t ha-1. The smallest demonstrable proof, i.e. the LSD5% was 0.22 t ha-1, which means that fertilization, in each case, significantly increased the yield. When analysing the effect of fertilization in the average of the hybrids and the different plant numbers, a yield of 5.61 t ha-1 could be detected, which value was 10.12 t ha-1 with N80+PK treatment and 11.61 t ha-1 with N160+PK treatment. Thus, it can be calculated that compared to the treatment without fertilization, the N80+PK treatment increased the yield by 4.51 t ha-1, while compared to the N80+PK treatment, the N160+PK treatment increased the yield by 1.49 t ha-1. In addition to agrotechnical factors, in maize production, the impact of the crop year is specifically of high importance.The average yield of hybrids (in the average of the different fertilizer treatments) was 6.81 t ha-1 in 2015, 11.86 t ha-1 in 2016 and 9.11 t ha-1 in 2017. When comparing the yield results against the precipitation data, it is clearly visible that the amount of rain fell in the January– October period is directly proportional to the average yield of maize. The effect of the crop year can be defined in a 5.05 t ha-1 difference in the yield. -
Improvement of black locust (Robinia pseudoacacia L.) growing under marginal site conditions in Hungary: case studies
129-133Views:317In Hungary, black locust (Robinia pseudoacacia L.) is considered as an important exotic stand-forming tree species growing mostly under unfavourable ecological conditions for forest management. Due to climate change effects, its importance is increasing in many other countries, too. As a result of a selection programme, new black locust clones were tested in clone trials. Juvenile growth of 12 micropropagated black locust clones in two plots series established at different dates were evaluated in central Hungary under marginal site conditions. At age of 7 the clone R. p. ‘Bácska’ (‘KH 56A2/5’), at age of 10 the clones R.p. ‘Homoki’ (‘MB17D3/4’) and ‘PV201E2/4’ appeared to be especially promising for mass production. Based on the data obtained from the performed trials, it can also be concluded that tissue culture can be considered as a suitable tool for propagating superior individuals and offers new prospects for the rapid cloning of selected genotypes used for plantation forestry.
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The influence of fertilization on the soil characteristics of a calcareous chernozem in a long term experiment
47-52Views:76In the long term fertilization experiment of the University of Debrecen, Centre for Agricultural and Applied Economic Science(CAAEC) (Debrecen Látókép), the effects of a 25-year-long fertilization were examined in terms of some chemical and microbiological properties of soil. With the growing doses of fertilizers, the available nutrient content of soil increased. At the same time the pH significantly decreased, while the hidden acidity increased. Moreover, the ratio between the soil bacteria and microscopic fungi, and the occurrence of microbes also changed. The number of sensitive physiological bacteria groups decreased dramatically. These changes indicate the reactions of living organisms; they correspond to the „resistance stage” of stress effects, but in the case of nitrifying bacteria, they reach the „exhaustion stage”.
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The role of green manure crops in Hungarian plant production
49-53Views:205According to the data of KSH (Hungarian Central Statistical Office), the sowing area of cereals in the crop year 2016 was over 2.56 million ha, on which winter wheat and maize were produced in a rate of around 50–50%. Regarding these data it is obvious that the domestic cropping structure has been simplified and become unilateral. This unfavourable crop rotation system causes several problems. The number of Hungarian livestock decreased in the past decades. The amount of manure was 24 million tons in 1960, but only hardly 4.5 million tons of organic manure was applied in 2016. Therefore, the importance of other possibilities, alternatives for organic matter recovery have become enhanced. This is especially important from the aspect of sustainable plant production. The fact that the European Union has introduced new directives for subsidiaries in 2015 has to be noted as well.
The objective of the set experiment is to find new technological solutions that are suitable for the execution of sustainable plant production by inadequate crop rotation, organic substance recovery conditions or under more unfavourable climatic conditions.
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The examination of flesh firmness in different apple varieties
103-107Views:176Of the different physical features of fruits, one important quality feature is flesh hardness. This is an important parameter to be considered when selling fresh apples, for the manufacturing industry and when entering into storage. In our research, we examined 7 apple varieties, in 2016 and 2017, from the establishment of KITE Zrt. in Derecske and the Horticultural Experimentation Site of Pallag (Pallagi Kertészeti Kísérleti Telep). The plantations took place in accordance with intensive apple production. In both years, samples were collected at the time ripening of the given apple variety. Of the examined apple varieties, 5 of them are resistant to scab (Gaia, Isaaq, Modí, Smeralda, Fujion), 1 is not resistant (Golden Reinders) and 1 (Pinova) is moderately susceptible to scab. The scab resistant apple varieties were correlated to the non-resistant Golden Reinders. Flesh firmness was measured with a fruit penetrometer in each of the seven apple varieties. In the first year a two-month storage experiment was carried out, where the flash firmness after harvest, and after one-month and two-month storage was measured. The results were assessed with the help of the IBM SPSS Statistics 20 program. The goal is to compare the apple varieties based on their flesh firmness, in particular with regard to the resistant varieties.
The Isaaq 8.81–8.82 N/cm2 , the Modí 9.18–9.59 N/cm2 and the Fujion 8.66–9.13 N/cm2 , all of which are scab resistant apple varieties, showed a significant initial flesh firmness. During storage, the Pinova apple variety flesh firmness changed the most favourably.
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Effects of different crop years and sowing date on maize yield
93-96Views:123We carried out the tests in the flood meadow soil formed on the alluvial cone of Nagykereki, Sebes-Körös belonging to the Bihar plane small region. The aim of the study was to analyse the effect of the different sowing date of maize on the yield trend based on a comprehensive study conducted for 6 years (2007–2012).
The sowing date of maize hybrids is a factor that significantly influences yield, however, its effect is not significant in each crop year. In the years when the date of sowing has a modifying effect, the reliable yield level can be reached with optimal sowing date management (24 April).
The advantage of early sowing (10 April) proved to be dominant in the year of 2012, the seeds were placed into the still wet soil therefore shooting was more balanced. Maize seeds sown at the time of optimal (24 April) and late (10 May) sowing dates were placed into the already dry soil, which deteriorated germination and the strength of early initial development that had an effect on the yield.
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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
81-85Views:123The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.
Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.
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Advancement of a Common Wheat (Triticum aestivum L.) Selection System
8-11Views:84All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect.