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Technologies developed to avoid frost damages caused by late frost during bloom in the fruit growing regions of Siófok and Debrecen
99-105.Views:1829The aim of the study was to fi nd out which of the methods used to avoid damages of late frosts would be the most effective for the fruit growing practice. Three technologies have been tested in the regions of Siófok and Debrecen-Pallag. The antifrost irrigation proved to be the most advantageous. For that purpose microjet sprayers are used, which are thrifty and do not need for that purpose large containers. With the aim to secure an even distribution of water, the sprayers are distributed on three levels: above and inside of the crown as well as on the level of trunks. On a large scale, a single microjet above the crown level would be suffi cient. By means of a detailed analysis served to set the optimum intervals between spraying phases: with each 15, 10, 5 and 3 minutes during half a minute. The synchronous presence of water and ice below the freezing point, the released freezing heat plus the water used much above the freezing point (9–10 °C) altogether maintains the temperature above around 0°C near the fl owers or growing fruitlets, meanwhile, the surrounding air cools down to –8 °C. The effectiveness of the generally used anti-frost would be increased substantially by the former application of cooling irrigation, which delays the blooming date. The Frostbuster represents a new technology developed for the same purpose of frost defence. It uses butane burning and produces high (80-90°C) temperature with a strong blast of air. On the protected area sensors have been distributed for measuring temperature and relative humidity. The means of the measurements proved a rise of temperature, which was suffi cient to save the fruit trees until the difference is less than –3–3.5 °C. Our analyses stated that paraffi n candles avert the frost until –4 °C. Its success depends largely on the intensity of air movements. A small wind would be enough to frustrate the effect. The results show clearly the utility of Frostbuster and paraffi n candle combined is approved.
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Applicability of 3D laser scanning in precision horticulture
55-58.Views:6251Due to the technological development, remote sensing instruments and methods have become widespread in all segments of life (from precision agriculture through architecture to medicine). Among the innovative development of remote sensing instruments the 3D laser scanner is of outstanding importance. Horticultural applicability of terrestrial laser scanning technique is a new innovation in the precision agriculture. The structure of trees and branches, the canopy extension, the fruit yield, which can help to recognize some biophysical parameters, can be determined. The examination was carried out with Leica ScanStation C10 terrestrial laser scanner in the Study and Regional Research Farm of the University of Debrecen near Pallag. In this article the measuring principle, the parameters and horticulture applicability of the terrestrial laser scanner are presented.
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Cytokinins affect the stomatal conductance and CO2 exchange of in vitro apple leaves
25-28.Views:562Effects of different cytokinin supplies including two types of aromatic cytokinins, such as benzyl-adenine, and 3-hydroxybenzyladenine applied at two different concentrations (2.0 and 6.0 μM) were studied on water and gas exchange parameters in in vitro apple leaves of ‘Royal Gala’ and ‘Freedom’ scions after 3 weeks of culture. Cytokinin supply affected the stomatal conductance of water vapour, transpiration rate and the sub-stomatal CO2 concentration of leaves. Effect of cytokinin depended on its applied type and concentration, moreover on the apple scion. According to the results, the rate of CO2 exchange itself is not usable for characterization of function of photosynthetic apparatus of in vitro leaves. However, measurements of stomatal conductance of water vapour and transpiration rate seemed to be good indicators for stomatal behaviour of in vitro apple leaves.
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Interaction impact of drought stress, nutrient-deficient water, and seed-borne pathogen (Alternaria alternata) on germination and vigor of two tomato varietiesination and vigor of two tomato varieties
48-53.Views:382Tomato (Solanum lycopersicum) is considered one of the leading vegetable plants in the world. This study evaluated the germination and vigor capabilities of ‘Marmande’ and ‘Kecskeméti Jubileum’ varieties under different conditions, including drought stress, nutrient-deficient water, and the effect of seed-borne disease caused by Alternaria alternata when prime and non-prime with salicylic acid. The experiment was conducted in the laboratory in 2023 at Agricultural and Food Sciences and Environmental Management Faculty of University of Debrecen. Results indicated that the ‘Kecskeméti Jubileum’ variety exhibited a superior strength to ‘Marmande’ in the seedling’s dry weight, seedling growth rate, and vigor index under nutrient-deficient water. Nevertheless, when applying levels of drought, the ‘Marmande’ variety had a higher viability rate of 62.5% in comparison with another variety, and the germination rate of the two tomato varieties is at 85.5% in 5% concentration but decreased progressively when exposed to a higher drought concentration of 10%. When using 3 ml of salicylic acid during germination stages, the seedling vigor index of ‘Marmande’ shows a greater index at 165 compared to ‘Kecskeméti Jubileum’, just 108 under the infection of Alternaria alternata. The results of the examination of drought stress, and the effect of Alternaria alternata, one cause of seed-borne pathogens, showed that the percentage germination and vigor ability of the ‘Marmande’ variety performed better than ‘Kecskeméti Jubileum’ under the same conditions.
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Ecological diversity of Hungarian medicinal and aromatic plant flora and its regional consequences
10-19.Views:401During the last century the medicinal and aromatic plant sector has became a successful part of the Hungarian Agriculture. Some of the national products have been accepted as a special Hungarian ones ("Hungaricum"), evaluated on the world market, respectfully. By the estimates the cultivation area of medicinal and aromatic plants increased up to 37,000-42,000 hectares and considerable amount — about 10, 000-15, 000 tonnes of dry biomass — are produced by utilisation of Hungarian indigenous flora, year by year.
In the present work ecological requirements of 97 collected and 55 cultivated medicinal and aromatic plants are characterised. Based on the analysis of -Ts (temperature regime values) about 63 per cent of cultivated species came from Submediterranean and Mediterranean type of habitat, originally, while the majority of collected plants (61.8 per cent of them) prefer the deciduous forest conditions. The differences between collected and cultivated species are appreciable too, if the distributions of their characteristic water regime ('W' values) are compared. The majority of cultivated species require dry (moderate dry) and fresh (moderate fresh) habitats, while the amplitude of water requirement of collected species is much more wide-ranging.
The regional specialisation of Hungary according to production of medicinal and aromatic plants is known from the beginnings of the 20th century. As a result of spontaneous process seven well-defined production areas were developed. The relationship between regions, their climatic conditions and spectrum of species produced there are analysed.
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The utilisation of nutrient elements in soilless cultures of vegetable production
47-49.Views:447Vegetable production in greenhouses may impair the ecological balance of the environment substantially as far as being uncontrolled. Soilless cultures especially should be handled thoughtfully. A fraction of the nutrients administered, more than 25-30%, is doomed to be lost in an open system, and the resulting ecological risk is accompanied with increasing costs of the production. Experiments have been conducted with the purpose of estimate the amount of nutrients involved. According to the results, as a mean, 30-80 per cent of the main nutrients was utilised. The rate of nutrient utilisation is influenced by the plant species involved as well as by the circumstances of production. One of its most important components is the irrigation, which determines the amount of overflow and of its salt content.
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Effects of salinity stress induced by hot spring water on tomato growth, yield and fruit quality under hydroponic cultivation in Japan
23-28.Views:381The objective of this research was to test hot spring water as a source of salt to improve tomato quality under the hydroponic system. This research was conducted at Yamagata University, in Japan from February to July 2017. Salt stress was induced using salts of hot spring wastewater collected from Yupoka Onsen (Tsuruoka, Japan). The treatments were EC 2, 4, 8 12, and 16 mS/cm which were arranged in a Randomized Complete Block Design (RCBD) with five replications. Tomato plants were grown at EC 2 until flowering and then subjected to different EC concentrations until harvesting. The data were collected on plant growth parameters and fruit quality. Fruits were harvested at the red stage until the 5th truss. The results showed that fruits' Soluble Solids Content, organic acid, Nitrate contents and Sugar: Acid ratio increased significantly at EC 16 mS/cm and in the upper trusses compared to EC 2 and in the lower trusses. In contrast, fruit weight significantly decreased at EC 16 mS/cm and in upper trusses. Leaf thickness, size and SPAD, and specific leaf weight significantly declined at EC 16 mS/cm and upper leaves compared to EC 2 and in lower leaves. Plant height started to decline significantly after three weeks of treatment at EC 16 compared to EC 2.
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The atmospherical drought as a decisive factor of yield in the main sour cherry varieties of Hungary
121-125.Views:1231Atmospheric drought causes heavy diffi culties of water supply in most fruit species grown in Hungary, although the modern, intensive plantations are already equipped with irrigation. The use a dripping systems are widely applied, therefore nothing was done to avert the risk of atmospheric drought. In excessively dry seasons the reduction of yields is often due to atmospheric drought. Present study aims to utilise measured data of meteorological parameters (relative air humidity and temperature) to develop an index to characterise drought and measure its effect on fruit yield. Causes influencing yields are multiple. Phytosanitary problems are combined with defi cits of water supply. Water deficit of the soil is avoided by dripping irrigation, but the atmosphere is infl uenced by sprinklers only. Atmospherical drought increases the transpiration of the trees intensely and causes reduction of photosynthetic activity, consequently impairs the yield. Applying the index developed in a plantation of 6 sour cherry varieties grown in Hungary (Meteor, Nefris, Pándy, Újfehértói fürtös, Kántorjánosi, Debreceni bôtermô), we measured the specific yields (yield per volume of tree crown) during the period 1989–2011 using the meteorological database of the growing site. Additionally, other parameters characterising the drought are compared and searched for a method most reliable for judging the specific yielding capacity of sour cherry varieties. The results proved convincingly the utility of the index, especially for the varieties Pándy and Újfehértói fürtös. Comparison with other indices expressing the effects of drought revealed the superiority of our index, which will be applied in the future to express the risk of atmospheric drought.
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Influence of time of planting on yam performance and pest infestation in humid rainforest of southwestern Nigeria
77-84.Views:87Yams (Dioscorea spp.) are annual or perennial tuber-bearing and climbing plants that belong to the family Dioscoreaceae. Only six principal species are grown for human consumption, while several others are produced for medicinal purposes. This study was designed to determine the influence of time of planting on yam performance and pest infestation in humid rainforest of southwestern Nigeria. The experiment was conducted in Randomized Complete Block Design (RCBD) with three treatments replicated five times. The planting materials are dipped into wood ash and allowed to dry up. The number of yam setts required for this work is 4 pieces, each of white yam sett, seed yam, and 4 pieces of water yam setts for the different planting times. The planting is done in five (5) phases. In each phase 12 heaps are planted, 4 white yams (seed yam), 4 yam setts (white yam) and 4 water yam setts. No significant difference was observed in 50% sprouting both in treatments and replication. There is significant difference in yield both in treatment and block. Yam planted in April (R5) has the highest yield with 3.7 kg, followed by those planted in ending March (R4) with 2.83 kg, followed by those planted in mid-February (R1) with 1.43 kg, then those planted in February ending (R2) with 1.2 kg and those planted in mid-March (R3) with 1.17 kg respectively. The highest level of destruction was witnessed in white seed yam planted in Mid-March and Mid-April as well as white yam sett planted in Mid-February. The lowest level of destruction was found in water yam sett planted in February ending. Shifting planting date can reduce larval survival and pest populations in yam tuber.
Keywords: Dioscorea spp., pest infestation, destruction, sprouting, yam performance.
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Effect of Foliar Nutrition on Post-Harvest of Onion Seed under Sandy Soil and Saline Irrigation Water Conditions
85-92Views:512Foliar application has been determined to be an effective nutrients delivery strategy in vegetable and fruits. The enhancement of vegetable and fruit yields affected by foliar nutrients application has been recognized in previously conducted studies with perennial tree crops. The efficiency of foliar nutrition is dependent on soil, climate, fertilizer and the amount of nitrogen used. There is no sufficient information concerning cooperation of foliar nutrition with all nutrients form as well as the rates of these nutrients fertilization in vegetable
and fruit crops. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on inflorescences diameter, flower stalk length, number of seed stem /plant, weight of 1000 seed, germination percentage, seed yield, moisture content, catalase , peroxidase activity and malondialdehyde content of onion seeds. The plants
sprayed with union Zn, union Mn, union feer, shams k, elga 600, boron, and amino x had the highest vegetative growth parameter, germination percent and enzyme activity. The plants sprayed with union Zn, union feer, shams K, magnesium, caboron, hummer and amino X had the highest seed yield ha-1. The seeds were stored for one year to study the effect of different commercial compounds and storage temperatures on germination, moisture content and change in antioxidant enzymes activities of onion seeds during the storage period. Storage at cold temperature showed higher germination percent, moisture content and lower malondialdehyde content than storage at room temperature. The treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer and amino x had the highest germination percent. -
Structural differences arise between fruit cuticles of two apple cultivars during long term cold storage
26-29Views:681Apple fruits are covered by hydrophobic cuticle that provides protection against desiccation, pathogens, excessive water absorption and radiation. The features of cuticle affect the quality and storability of the fruits. It was aimed to evaluate the correlation between peel ultrastructure and weight loss in fruits covered by waxy bloom (cv. Florina) and without bloom (cv. Red Rome van Well) during 4-month cold storage. SEM (scanning electron microscopy) was used for visualization of the fruit surface and fruit weight was also measured. Several studies have revealed that there may be correlation between the ultrastructure of cuticle and its water permeability. Our results confirmed this phenomenon in case of two cultivars. The weight loss per fruit surface area unit of ‘Florina’ was found significantly higher than that of ‘Red Rome van Well’. At the same time the cuticular surface of the fruits contained more micro-cracks in case of the former cultivar. These data confirmed the relationship between the density of cuticular micro-cracks and the water vapour permeability. We concluded that this feature is more significant than the amount of natural waxy bloom on fruit surface.
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Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
69-77.Views:398The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.
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Composition and storage of pear cultivars from Nagykanizsa
63-68.Views:1521The composition of five pear varieties (‘Abate Fètel’, ‘Bosc’, ‘Williams’, ‘Conference’, ‘Packham’s Triumph’) grown in Nagykanizsa was investigated in three consecutive years (2008, 2009, 2010). A storage experiment was performed in 2008. Four winter pear cultivars were kept in an ULO store for four months and their parameters measured after two and four months. The parameters tested were: size, weight, water soluble solids, titratable acidity, glucose, fructose, sucrose, water soluble pectin, total polyphenols, free radical scavenging capacity, copper and zinc content. The fruits of ‘Conference’ and ‘Bosc’ varieties were found to contain the highest sucrose and total sugar content, while ‘Abate Fétel’ had the lowest sucrose and highest glucose levels among cultivars tested. ‘Williams’ pear was the most acidic. Brix, total sugar, sucrose and water soluble pectin were decreased during storage. Titratable acidity slightly decreased in fruits of Conference pear. Polyphenols and free radical scavenging capacity did not show a significant change during storage.
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Efficacy of selected botanical extracts against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) on tomato
31-36.Views:675Tuta absoluta Meyrick, also known as the tomato leaf miner, is a destructive pest that feeds on tomato plants, causing significant economic losses to tomato farmers globally. Traditional methods of controlling Tuta absoluta involve the use of synthetic pesticides, but these methods have drawbacks such as environmental pollution, development of resistance by the pest, and negative effects on non-target organisms. As a result, there is a growing interest in the use of botanical extracts as alternative and safer pest control options. Three bioassay experiments were conducted to test the insecticidal, antifeedant and repellency effects of botanical extracts on Tuta absoluta. The experiments were conducted as completely randomized design (CRD) with three (3) replicates. There were eleven treatments; pyrethrum, neem, warbugia, each at different concentration levels of (8%, 10%, 12%) and controls positive (Indoxacarb 150g/L) and negative (distilled water). In all the bioassay conducted, the effects of all treatments increased over time, except for distilled water control. Synthetic chemical Indoxacarb exhibited significantly stronger insecticidal properties compared to neem, warburgia and pyrethrum extracts. Application of neem at 8%, 10% and 12% and pyrethrum at 12% had higher antifeeding effect on Tuta absoluta compared to the other treatments while warburgia at 8% concentration and indoxacarb had the lowest repellency effect compared to the other plant extracts.
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The effect of cooling irrigation on the blooming dynamic of plum
57-59.Views:1211The objective of the present study is to explore the effect of cooling irrigation (aspersion) on the beginning of bloom and on the micro-climate of the plantation. The results show that the water sprayed in the orchard by micro-jet influenced decisively the temperature of the plantation. At higher temperatures (around 20 °C), the drop of temperature may attain 5–7 °C. A low relative humidity of the air may increase the relative effect, The frequent repetition (20 minute intervals) may keep the temperature low also in the buds. The beginning of bloom may delayed for more than ten days. The dynamics of blooming was characterised by a logistic curve in the treated as well as in the control plot. In the treated plot, the curve was steeper than in the control in spite of equal temperatures measured in the plots. Under our (Hungarian) climatic conditions, the method is successfully used to delay blooming dates. The main result is the diminution of the frost damage in the spring and the security of yield. The costs and water requirement should be calculated later.
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Composted and natural organic materials as potential peat-substituting media in green pepper growing
31-35.Views:291Peat is the most favourable and usable medium in vegetable and ornamental plant forcing but because of the intensive exploitation peat resources decreased significantly all around the world. As peat-reserves run out the use of pine bark, composts and other organic materials spread in horticultural growing. In this study we compared the suitability of peat-based media to pine bark and two types of composts. We examined the effect of different organic materials on the growth and yield of green pepper (Capsicum annum L., variety Danubia). We found that the most developed plants were grown in peat-based media and pine bark. The average fruit weight was the highest in low moor-high moor peat mixture and pine bark. The plants which were grown in composts fell short of our expectations.
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Summer pruning of sweet cherry trees and an inquiry of winter frost damages
41-44.Views:10637One of the most demanded research projects is the intensification of fruit production. The use of dwarfing stocks is a moderate solution as their effect is scarcely satisfactory. Climatic conditions of Hungary are continental in Eastern Europe, where Atlantic and Mediterranean effects are interacting with the continentals in a kind of basin with characters of its own. Capricious meteorological episodes are often disturbing the security of development and fruiting of trees:
• winter frosts are damaging the cambium and fruiting structures of trees
• late spring frosts destroy cambium and flowers
• early autumn frosts hurt the leaves
• excessive precipitation impairs the growing fruits
• drought periods during the summer caused water stress disturbing water husbandry.Vigorous stocks still prevail in the practice, and they ought to withstand challenges of weather hazards. The strong vigour of plants delaysthe process of senescence and the tendency of getting bald, and regeneration of plants is a sign of vitality. In present research, the trees have been trained on vigorous Prunus mahaleb stocks. Summer pruning was one of the important tools of intensive growing techniques. They were compared with traditional techniques and with plastic foil protected trees observing the vegetative as well as generative growth of them.
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The effect of soil coverage on the soil quality
77-80.Views:320In this study we carried out experiments with soil samples originated from the Haschberg variety elderberry orchard and wild-growing plants from Szigetcsép Experimental Farm.
During the examinations we wanted to know how the black polyethene foliar soil coverage influences the quality of soil and the distribution of different nitrogen forms.
We studied the individual covered (foliar soil coverage for every plant), the full covered (foliar soil coverage was laid in the rows) and the uncovered threatments.
During the experiments we pointed out that the foliar coverage influences the condition of the soil unfavourably. Because of the coverage there is less air flow in soils, the lack of oxygen leads to degradating of soil life and results humus materials of poor quality.
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Study of genetic resources for Powdery mildew resistance by biochemical and physiological parameters on peaches and nectarines in Romania
57-61.Views:281During 1997-2002, studies on the physiological and biochemical changes due to powdery mildew attack in peach and nectarine cultivars and hybrids belonging to different classes of resistance had been performed at the Research Station for Fruit Tree Growing (RSFTG) Baneasa Bucharest, located in the southern part of Romania. The study was based on disease assessments in the field and on other diagnosis criteria.
The southern part of Romania has favourable ecological conditions for growing peach and nectarine but trees have been attacked by various pathogens such as Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. persicae Woronichin fungus for a long time. The presence of the pathogen causes important changes in the quantity and quality of fruits and affects the metabolism of trees.
This study was focused on several physiological and biochemical parameters such as photosynthesis and respiration intensity along with catalase activity; and changes in some biochemical compounds such as free, total and linked water content, dry matter, nitrogen and protein content, tannin levels. The aim was to find possible correlation among the above parameters and the biotic stress induced by the powdery mildew fungus calculated as attack degree (AD%).
Results showed that photosynthesis diminished significantly in the case of the attacked phenotypes, the respiration was more intense in the infected cultivars and catalase proved to be less intense in the affected plants. The total water content significantly increased in contrast to the dry matter and tannin contents, which greatly reduced in the case of resistant cultivars such as 'Victoria', Vectared 7', 'KB 11-40'. The total nitrogen and protein levels showed less influence on resistance to powdery mildew on all cultivars. All genetic material found resistant to powdery mildew is currently used in peach and nectarine breeding programs in Romania.
Further studies focused on isolation and characterization of resistance genes for powdery mildew resistance will be done in the future based on data collected during several years.
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Research of the elasticity of transplant - growing substrates after watering
67-69.Views:337It gets more and more popular to grow transplants in different trays for the field vegetable growing. The best transplant-growing substrates in the world are made of peat. The peat is applied to provide an optimal supply of plants with water and air. To improve the water regulation and the structure of the mixtures there are often mineral matters used in different amounts. By measuring the physical properties of soil mixtures based on peat, the flexibility can be measured by Stable Micro System type table penetrometer. Our measuring confirmed the increase of bulk and flexibility of different kinds of peat by watering.
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Utilisation of nutrients by cucumber plants oil rockwool substrate
117-121.Views:243Cucumber production by soil less culture on rockwool substrate in Hungary is an open system regarding its water and nutrient supply. Environmental and economical reasons require the recycling of nutrients of the system. Experiments have been planned in order to estimate the utilisation of individual nutrient elements applied. All around the vegetation period, chemical analyses monitored the depletion of the elements initially administered by sampling the solutions at successive phases from the injection to the overflow. The results have been compared with earlier experiences refering to general rules. It has been stated that the intensity of photosynthesis is decisive in determining the composition of the overflow. The less changes are observed during the period of frequent watering.
The absorption of the nutrient elements varied between 25-51% deperiding on the individual elements. The differences are significant. Further examinations are needed in order to clear:
- which are the main elements of technology, which are decisive in utilisation of nutrients
- what are the possibilities of the secondary utilisation of nutrients.
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Effects of Foliar Nutrition on Onion Seed Storage under Modified Atmosphere Packages
93-100Views:474Modified atmosphere packaging (MAP) and controlled atmosphere storage techniques to reduce the oxygen around the food are largely used for the preservation of fresh produce. There have been great technological advances in this area of preservation, particularly as it refers to improving the quality and shelf-stability of highly perishable food products, such as produce. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on onion seeds storage under modified atmosphere packages. Germination percent of seeds decreased in 5°C than storage in room temperature. Germination percent of seeds was gradually decreased with increasing the storage period. Packaging treatments had a significant effect on germination percent of seeds. All the packaging treatments had the higher germination percent than the paper package (control). The highest germination percent after 12 months of storage was recorded for the treatment with non perforated polypropylene in room temperature and polyethylene and non perforated polypropylene in 5°C. Catalase activity decreased with the prolongation of storage period. The non perforated polypropylene package had the highest catalase activity. The treatment with non perforated polypropylene had the highest catalase activity after 12 months of storage in both room and 5°C temperatures. Peroxidase activity of seeds was gradually decreased with increasing the storage period. The highest peroxidase activity after 12 months of storage was recorded in non perforated polypropylene in both storage temperatures.
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Micropropagation of Rudbeckia hirta L. from seedling explants
105-108Views:502We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strenght solid Murashige and Skoog (1962) culture medium containing 10 mg/1 of kinetin or 2 mg/I of kinetin + 0.1 mg/1 of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/1 of IAA. After four weeks of incubation we obtained elongated shoots which we separated and inoculated into a new culture medium and we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respect of breeding.
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The necessity and possibilities of irrigation in fruit growing under conditions of Hungary
93-94.Views:1349Climatic and soil conditions are highly suitable for most temperate fruit species and promise profitable yields with good quality. An accurate choice of the growing site is, however, decisive because of the wide range agro-climatic variation an soils within the country. One of the most important factors is the annual precipitation which does not exceed, in general, 700 mm. The aims of irrigation practices are, succinctly speaking, the improvement of quantity and security of yields and the guarantee of quality. The relative importance of those criteria changes according to the fruit species. In up to date apple, pear and cherry production, micro-irrigation systems are mainly considered. According to recent experiences, the micro-jet type of water distribution should be preferred to the dripping system. In cherries, the choice of the method is motivated by the need to prevent fruit cracking. Most of the peach and apricot plantations are located on the dry and moderately dry regions of the country. Because of the late freezes, the improvement of security is crucial. There the investment of irrigation systems should concentrate to the possibility of anti-freeze sprays. High water requirements of plums are met in Hungary by irrigation where the method should be decided at the plantation and adapted to the harvesting procedure which could be mechanised or (in high density plantations) picked by hand. Sour cherries are perhaps the less dependent on watering under Hungarian conditions. Yields in small fruits: currents, gooseberries, raspberries and strawberries could be increased by irrigation to 40-50 % and may improve quality too. In those cultures the system of moving flexible wing tubes are considered to be the best irrigation technique.
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In vitro comparative study of two Arundo donax L. ecotypes’ selenium tolerance
119-122.Views:641Selenium tolerance of two somatic embryo-derived Arundo donax L. ecotypes (Blossom, 20SZ) were compared in in vitro culture. Sodium-selenate (1 – 100 mg L-1) as known the most phytoavailable selenium form and the less studied red elemental nanoselenium (100 mg L-1) were applied as selenium treatments. Basis on the results Blossom ecotype seemed to be more sensitive to the sodium-selenate than 20SZ. Inhibiting effect of selenate was effectuated above 10 mg L-1 in case of Blossom, which was manifested in decreased survival rate and growing parameters. Contrast to this 20SZ could tolerate the selenate ≤ 20 mg L-1 without any toxic symptoms. Lower selenate tolerance of Blossom could be explained with higher selenium accumulation. Both of two ecotypes could also uptake and accumulate the red elemental nanoselenium however in much less extent compared to selenate.
